Click here to switch to the map view.

The map label for this gene is alr1 [H]

Identifier: 188584948

GI number: 188584948

Start: 324660

End: 325832

Strand: Direct

Name: alr1 [H]

Synonym: Nther_0307

Alternate gene names: 188584948

Gene position: 324660-325832 (Clockwise)

Preceding gene: 188584947

Following gene: 188584949

Centisome position: 10.26

GC content: 36.06

Gene sequence:

>1173_bases
ATGTCTGAATCTATTCCTGTAAGACCGACTTTTTCTGAAATTAACTTGGACAATCTTTCTTATAACTTTAATCGGATAAG
AGAGCATGTGAACAATAAAAAAATCATGGGTATCATTAAAGCTGATGGTTATGGTCATGGAGCTGTTGAAATTGCCGAAG
AACTATTGAATCAAGGTGTAGATTATTTAGGTGTAGCTATTTTAGACGAAGCTGTGGAACTAAGGAAAGCAGGAATCGCT
GCTCCTATTTTAATTCTTGGCTATACACCTGTAGAGCATGTTGATGTATGTTTGAAATATGGTATCACTCCCACAATTTA
TACCTATGAAATGGCAGAAGCCCTTTCTCAAACCGCTAGAAAAATGGGGAAAACTGCCTTTGTTCACGTAAAAATTGATA
CAGGCATGGGTCGGATAGGAATAGCTCCAGAAAATAGTGCTGAGTTTGTCAAAAATATTAGCAAATTGCCATCCTTAGAA
GTGGAAGGTGTTTTCACGCATTTTTCAGTAGCAGATGAGGAAGATAAGACTTATACACAACAGCAAATCAATAGCTTTGA
AAAAGCCTTAAAGATGTTAGAAGATGAAGGTATTGAAATTCCTCTAAAACATGCTGCTAACAGTCCTGCTACTATTGACT
TGCCAGATGCATATTTTGATATGGTTAGGGTTGGACTAGCCTTATTCGGTTTATATCCTTCTCCGCACATGAAGGAAAAA
ATTTCACTAAAACCAGTAATGAGTCTTAAAACTCAAGTATCCCATGTGAAACAAGTTCCCAAGGGAACTTCTATAAGTTA
TGGTAGGAAGTTTATTACTGAAGATGAAAGTATTATTGGCACATTACCAGTTGGTTATGCAGATGGCTTTACACGATTAC
TATCTAATATAGGTGAAGTCATAGTTAATGGTAAAAGAGTGCCCATTGTAGGAGCCATATGTATGGACCAATGCATGTTC
CTAGGTGACGATGTACCGGAGATTGGAATCGGAGATGAAGTAGTTATTATTGGTGAACAAGATGGTACTCAAATTACAGT
TGAGGATATAGCTGATAAACTAGGAACTATCAACTATGAAATAGTTACTATGGTGGACAAACGAGTGCCACGTTTATATC
TAAAAAATGGCAAATTAATCAAAAAGAAATCTTTAAATAGCAAAATGTATTAG

Upstream 100 bases:

>100_bases
TAATTTTCTTTTACATTGAATATATTATTTAGGTGTAATTAATTTGATTATTTACAGGACTACCTTTATAATTGTGCATA
ATGAGGTGTGAGGTGACAAT

Downstream 100 bases:

>100_bases
TTTGTAACAAAATGTGAATGCTAGTCTGTATAAGGCGCTATATTTTGTTGCATAATGATAATGAGCTAGTTATAATATTC
CTTGTCCTTGTTTTGTATTT

Product: alanine racemase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 390; Mature: 389

Protein sequence:

>390_residues
MSESIPVRPTFSEINLDNLSYNFNRIREHVNNKKIMGIIKADGYGHGAVEIAEELLNQGVDYLGVAILDEAVELRKAGIA
APILILGYTPVEHVDVCLKYGITPTIYTYEMAEALSQTARKMGKTAFVHVKIDTGMGRIGIAPENSAEFVKNISKLPSLE
VEGVFTHFSVADEEDKTYTQQQINSFEKALKMLEDEGIEIPLKHAANSPATIDLPDAYFDMVRVGLALFGLYPSPHMKEK
ISLKPVMSLKTQVSHVKQVPKGTSISYGRKFITEDESIIGTLPVGYADGFTRLLSNIGEVIVNGKRVPIVGAICMDQCMF
LGDDVPEIGIGDEVVIIGEQDGTQITVEDIADKLGTINYEIVTMVDKRVPRLYLKNGKLIKKKSLNSKMY

Sequences:

>Translated_390_residues
MSESIPVRPTFSEINLDNLSYNFNRIREHVNNKKIMGIIKADGYGHGAVEIAEELLNQGVDYLGVAILDEAVELRKAGIA
APILILGYTPVEHVDVCLKYGITPTIYTYEMAEALSQTARKMGKTAFVHVKIDTGMGRIGIAPENSAEFVKNISKLPSLE
VEGVFTHFSVADEEDKTYTQQQINSFEKALKMLEDEGIEIPLKHAANSPATIDLPDAYFDMVRVGLALFGLYPSPHMKEK
ISLKPVMSLKTQVSHVKQVPKGTSISYGRKFITEDESIIGTLPVGYADGFTRLLSNIGEVIVNGKRVPIVGAICMDQCMF
LGDDVPEIGIGDEVVIIGEQDGTQITVEDIADKLGTINYEIVTMVDKRVPRLYLKNGKLIKKKSLNSKMY
>Mature_389_residues
SESIPVRPTFSEINLDNLSYNFNRIREHVNNKKIMGIIKADGYGHGAVEIAEELLNQGVDYLGVAILDEAVELRKAGIAA
PILILGYTPVEHVDVCLKYGITPTIYTYEMAEALSQTARKMGKTAFVHVKIDTGMGRIGIAPENSAEFVKNISKLPSLEV
EGVFTHFSVADEEDKTYTQQQINSFEKALKMLEDEGIEIPLKHAANSPATIDLPDAYFDMVRVGLALFGLYPSPHMKEKI
SLKPVMSLKTQVSHVKQVPKGTSISYGRKFITEDESIIGTLPVGYADGFTRLLSNIGEVIVNGKRVPIVGAICMDQCMFL
GDDVPEIGIGDEVVIIGEQDGTQITVEDIADKLGTINYEIVTMVDKRVPRLYLKNGKLIKKKSLNSKMY

Specific function: Provides the D-alanine required for cell wall biosynthesis [H]

COG id: COG0787

COG function: function code M; Alanine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alanine racemase family [H]

Homologues:

Organism=Escherichia coli, GI1790487, Length=366, Percent_Identity=30.0546448087432, Blast_Score=168, Evalue=5e-43,
Organism=Escherichia coli, GI1787439, Length=368, Percent_Identity=28.5326086956522, Blast_Score=167, Evalue=9e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000821
- InterPro:   IPR009006
- InterPro:   IPR011079
- InterPro:   IPR001608
- InterPro:   IPR020622 [H]

Pfam domain/function: PF00842 Ala_racemase_C; PF01168 Ala_racemase_N [H]

EC number: =5.1.1.1 [H]

Molecular weight: Translated: 43000; Mature: 42869

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS00395 ALANINE_RACEMASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSESIPVRPTFSEINLDNLSYNFNRIREHVNNKKIMGIIKADGYGHGAVEIAEELLNQGV
CCCCCCCCCCHHHCCCCCCCCCHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHCCH
DYLGVAILDEAVELRKAGIAAPILILGYTPVEHVDVCLKYGITPTIYTYEMAEALSQTAR
HHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHCCCCEEEHHHHHHHHHHHHH
KMGKTAFVHVKIDTGMGRIGIAPENSAEFVKNISKLPSLEVEGVFTHFSVADEEDKTYTQ
HCCCEEEEEEEEECCCCEEECCCCCHHHHHHHHHHCCCCEEEEEEEEEEECCCCHHHHHH
QQINSFEKALKMLEDEGIEIPLKHAANSPATIDLPDAYFDMVRVGLALFGLYPSPHMKEK
HHHHHHHHHHHHHHHCCCEEEHHHCCCCCCEEECCHHHHHHHHHHHHHHCCCCCCCCCCC
ISLKPVMSLKTQVSHVKQVPKGTSISYGRKFITEDESIIGTLPVGYADGFTRLLSNIGEV
CCCCHHHHHHHHHHHHHHCCCCCCCHHCCHHCCCCCCEEEECCCCHHHHHHHHHHHHHHH
IVNGKRVPIVGAICMDQCMFLGDDVPEIGIGDEVVIIGEQDGTQITVEDIADKLGTINYE
HCCCCCCEEEHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCEEEHHHHHHHHCCCCEE
IVTMVDKRVPRLYLKNGKLIKKKSLNSKMY
EEEEHHHHCCEEEECCCCEEEECCCCCCCC
>Mature Secondary Structure 
SESIPVRPTFSEINLDNLSYNFNRIREHVNNKKIMGIIKADGYGHGAVEIAEELLNQGV
CCCCCCCCCHHHCCCCCCCCCHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHCCH
DYLGVAILDEAVELRKAGIAAPILILGYTPVEHVDVCLKYGITPTIYTYEMAEALSQTAR
HHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHCCCCEEEHHHHHHHHHHHHH
KMGKTAFVHVKIDTGMGRIGIAPENSAEFVKNISKLPSLEVEGVFTHFSVADEEDKTYTQ
HCCCEEEEEEEEECCCCEEECCCCCHHHHHHHHHHCCCCEEEEEEEEEEECCCCHHHHHH
QQINSFEKALKMLEDEGIEIPLKHAANSPATIDLPDAYFDMVRVGLALFGLYPSPHMKEK
HHHHHHHHHHHHHHHCCCEEEHHHCCCCCCEEECCHHHHHHHHHHHHHHCCCCCCCCCCC
ISLKPVMSLKTQVSHVKQVPKGTSISYGRKFITEDESIIGTLPVGYADGFTRLLSNIGEV
CCCCHHHHHHHHHHHHHHCCCCCCCHHCCHHCCCCCCEEEECCCCHHHHHHHHHHHHHHH
IVNGKRVPIVGAICMDQCMFLGDDVPEIGIGDEVVIIGEQDGTQITVEDIADKLGTINYE
HCCCCCCEEEHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCEEEHHHHHHHHCCCCEE
IVTMVDKRVPRLYLKNGKLIKKKSLNSKMY
EEEEHHHHCCEEEECCCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]