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The map label for this gene is nifU [H]

Identifier: 188584944

GI number: 188584944

Start: 320661

End: 321074

Strand: Direct

Name: nifU [H]

Synonym: Nther_0303

Alternate gene names: 188584944

Gene position: 320661-321074 (Clockwise)

Preceding gene: 188584943

Following gene: 188584945

Centisome position: 10.13

GC content: 35.27

Gene sequence:

>414_bases
ATGTATAATGAGAAAGTGATGGACCATTTTCAAAATCCGAGGAATGTGGGAGAGGTAGAAAGCCCTGATGGGGTTGGTGA
AGTAGGAAATGTAACTTGTGGAGATATTATGAGGATTAGCATTAAAGTTAATAACAATGAAGAAATAGAAGATATTAAAT
TTAAAACTTTTGGATGCGGAGCAGCTATTGCTACAAGCAGCATAGTAACTGAAATGGCAAAAGGAAAGCATATTAATGAA
GCTAGAGATATTAAGAATAAGGATGTAGCTGATGAATTAGATGGTTTACCAAAAAATAAGCTCCACTGTTCTAATTTGGC
TGCTGATGCTCTAACTAAGGCCATTAAAAATTATTTGGGAGAAGAAGATGACGAAGAACAAGAACATGAAGACGAATCCA
ATGAAACCCAGTAA

Upstream 100 bases:

>100_bases
TCATCGAAGCTGTTCCTGAAATAGTCAACAGATTAAGGGATATGTCTTCCATTTGGAAGGGTAAAGAAGCTAATTACAGT
AATTAATGGAGGGATGATAG

Downstream 100 bases:

>100_bases
AATCCATAAATTAATCTGAGTCAATATAAAATGAGATAGTATATGATAAATTAATTTCCATATTTTCCACTAAATACAAG
GAGTCCCTGCCTAAAAAGCA

Product: FeS cluster assembly scaffold protein NifU

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 137; Mature: 137

Protein sequence:

>137_residues
MYNEKVMDHFQNPRNVGEVESPDGVGEVGNVTCGDIMRISIKVNNNEEIEDIKFKTFGCGAAIATSSIVTEMAKGKHINE
ARDIKNKDVADELDGLPKNKLHCSNLAADALTKAIKNYLGEEDDEEQEHEDESNETQ

Sequences:

>Translated_137_residues
MYNEKVMDHFQNPRNVGEVESPDGVGEVGNVTCGDIMRISIKVNNNEEIEDIKFKTFGCGAAIATSSIVTEMAKGKHINE
ARDIKNKDVADELDGLPKNKLHCSNLAADALTKAIKNYLGEEDDEEQEHEDESNETQ
>Mature_137_residues
MYNEKVMDHFQNPRNVGEVESPDGVGEVGNVTCGDIMRISIKVNNNEEIEDIKFKTFGCGAAIATSSIVTEMAKGKHINE
ARDIKNKDVADELDGLPKNKLHCSNLAADALTKAIKNYLGEEDDEEQEHEDESNETQ

Specific function: May be involved in the formation or repair of [Fe-S] clusters present in iron-sulfur proteins (Potential) [H]

COG id: COG0822

COG function: function code C; NifU homolog involved in Fe-S cluster formation

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifU family [H]

Homologues:

Organism=Homo sapiens, GI56699456, Length=135, Percent_Identity=47.4074074074074, Blast_Score=131, Evalue=2e-31,
Organism=Homo sapiens, GI24307953, Length=132, Percent_Identity=46.969696969697, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI1788878, Length=120, Percent_Identity=55.8333333333333, Blast_Score=137, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI17543474, Length=120, Percent_Identity=53.3333333333333, Blast_Score=129, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6325122, Length=122, Percent_Identity=52.4590163934426, Blast_Score=128, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6324800, Length=122, Percent_Identity=50.8196721311475, Blast_Score=124, Evalue=5e-30,
Organism=Drosophila melanogaster, GI21355597, Length=121, Percent_Identity=53.7190082644628, Blast_Score=133, Evalue=3e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002871 [H]

Pfam domain/function: PF01592 NifU_N [H]

EC number: NA

Molecular weight: Translated: 15153; Mature: 15153

Theoretical pI: Translated: 4.27; Mature: 4.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYNEKVMDHFQNPRNVGEVESPDGVGEVGNVTCGDIMRISIKVNNNEEIEDIKFKTFGCG
CCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCHHEEEECCCCC
AAIATSSIVTEMAKGKHINEARDIKNKDVADELDGLPKNKLHCSNLAADALTKAIKNYLG
HHHHHHHHHHHHHCCCCCCHHHHCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHC
EEDDEEQEHEDESNETQ
CCCCCHHHHHCCCCCCC
>Mature Secondary Structure
MYNEKVMDHFQNPRNVGEVESPDGVGEVGNVTCGDIMRISIKVNNNEEIEDIKFKTFGCG
CCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCHHEEEECCCCC
AAIATSSIVTEMAKGKHINEARDIKNKDVADELDGLPKNKLHCSNLAADALTKAIKNYLG
HHHHHHHHHHHHHCCCCCCHHHHCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHC
EEDDEEQEHEDESNETQ
CCCCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]