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The map label for this gene is iscS [H]

Identifier: 188584943

GI number: 188584943

Start: 319468

End: 320646

Strand: Direct

Name: iscS [H]

Synonym: Nther_0302

Alternate gene names: 188584943

Gene position: 319468-320646 (Clockwise)

Preceding gene: 188584942

Following gene: 188584944

Centisome position: 10.09

GC content: 37.07

Gene sequence:

>1179_bases
ATGAATAAAATTTATCTTGATCATGGAGCTACAACTCCTATGCGTGAAGAAGTATTTGAAACAATGAAACCATATTTACA
GGATAATTTCGGAAATCCTTCCAGTATCCACAGCTTTGGAAGAGAAGTGAGAAAGGCAGTTGACGAGGCTAGAGAACAAG
TGGCCAATGCCATTAATGCTCAACCAGAGGAGATTATTTTTACTTCTGGTGGCACAGAATCGGATAATTTAGCTATTCAA
GGTGTTATTGAAAAAATAGGAGATAAAGGAAATCATATAATTACTACTCAGATTGAGCATCATGCTGTCTTAGATACTTG
TGAAGCAATGGAGGAAAAGGGATATAAAGTCACCTACTTACCAGTTGATAAATATGGTCTGGTTAATCCCAAGGACTTAC
AAGAAGCCATCAATGAAGACACTGTTTTAATTACTATCATGCATGGTAATAACGAGGTTGGAACTATTCAGCCAATCAAA
GAACTGGCTGAAATAGCTAAAGATAATGATGTTTATTTCCATACAGATGCAGTTCAGACAGTAGGGAATATCCCAATCGA
TGTTCAAGACTTGGGGATAGATATGTTAACTCTTTCAGCCCATAAATTTTACGGACCCAAGGGAATTGGCGCTTTATATA
TCAAAAAAGGCACTAAATTACAGAGAGTAGTACACGGAGGGGCTCAAGAACGAAAAATCAGGCCAGGAACGGAAAATGTT
CCAGGAATTGTAGGTTTAGGTAAAGCAATAGAATTAGCCACTGAAGAATTAGATACTAAAGGAAAACACATTCAAAAATT
ACGAGACAAATTAATTGAAGGTCTTTCTAAAGTAGAAGATAGTCGCTTGAATGGACACCCTGAACGCAGACTTCCCAATA
ATGTCAATATGAGTTTTGAATATATAGAAGGTGAATCCCTGTTATTAAACCTTGATATGAAGGGGATTGCAGCTTCTAGT
GGATCTGCTTGTACTTCTGGTTCTCTTGATCCGTCCCATGTGTTAATGGCAATGGGTTTAAGTCATCAAACTGCTCATGG
TTCTCTTAGATTGACATTAGGTAGAGATAATACCGAAGAGGAAATTGATTATGTCATCGAAGCTGTTCCTGAAATAGTCA
ACAGATTAAGGGATATGTCTTCCATTTGGAAGGGTAAAGAAGCTAATTACAGTAATTAA

Upstream 100 bases:

>100_bases
TTGGAATTGAGAGATAGAATATCTGAAGTATTAGATGAATTCACCTTGCAAGATCTAGCCGTTACAGATAATGATATCGA
AAAATTAAGGGGGGAACATA

Downstream 100 bases:

>100_bases
TGGAGGGATGATAGATGTATAATGAGAAAGTGATGGACCATTTTCAAAATCCGAGGAATGTGGGAGAGGTAGAAAGCCCT
GATGGGGTTGGTGAAGTAGG

Product: cysteine desulfurase NifS

Products: NA

Alternate protein names: NifS protein homolog [H]

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MNKIYLDHGATTPMREEVFETMKPYLQDNFGNPSSIHSFGREVRKAVDEAREQVANAINAQPEEIIFTSGGTESDNLAIQ
GVIEKIGDKGNHIITTQIEHHAVLDTCEAMEEKGYKVTYLPVDKYGLVNPKDLQEAINEDTVLITIMHGNNEVGTIQPIK
ELAEIAKDNDVYFHTDAVQTVGNIPIDVQDLGIDMLTLSAHKFYGPKGIGALYIKKGTKLQRVVHGGAQERKIRPGTENV
PGIVGLGKAIELATEELDTKGKHIQKLRDKLIEGLSKVEDSRLNGHPERRLPNNVNMSFEYIEGESLLLNLDMKGIAASS
GSACTSGSLDPSHVLMAMGLSHQTAHGSLRLTLGRDNTEEEIDYVIEAVPEIVNRLRDMSSIWKGKEANYSN

Sequences:

>Translated_392_residues
MNKIYLDHGATTPMREEVFETMKPYLQDNFGNPSSIHSFGREVRKAVDEAREQVANAINAQPEEIIFTSGGTESDNLAIQ
GVIEKIGDKGNHIITTQIEHHAVLDTCEAMEEKGYKVTYLPVDKYGLVNPKDLQEAINEDTVLITIMHGNNEVGTIQPIK
ELAEIAKDNDVYFHTDAVQTVGNIPIDVQDLGIDMLTLSAHKFYGPKGIGALYIKKGTKLQRVVHGGAQERKIRPGTENV
PGIVGLGKAIELATEELDTKGKHIQKLRDKLIEGLSKVEDSRLNGHPERRLPNNVNMSFEYIEGESLLLNLDMKGIAASS
GSACTSGSLDPSHVLMAMGLSHQTAHGSLRLTLGRDNTEEEIDYVIEAVPEIVNRLRDMSSIWKGKEANYSN
>Mature_392_residues
MNKIYLDHGATTPMREEVFETMKPYLQDNFGNPSSIHSFGREVRKAVDEAREQVANAINAQPEEIIFTSGGTESDNLAIQ
GVIEKIGDKGNHIITTQIEHHAVLDTCEAMEEKGYKVTYLPVDKYGLVNPKDLQEAINEDTVLITIMHGNNEVGTIQPIK
ELAEIAKDNDVYFHTDAVQTVGNIPIDVQDLGIDMLTLSAHKFYGPKGIGALYIKKGTKLQRVVHGGAQERKIRPGTENV
PGIVGLGKAIELATEELDTKGKHIQKLRDKLIEGLSKVEDSRLNGHPERRLPNNVNMSFEYIEGESLLLNLDMKGIAASS
GSACTSGSLDPSHVLMAMGLSHQTAHGSLRLTLGRDNTEEEIDYVIEAVPEIVNRLRDMSSIWKGKEANYSN

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=388, Percent_Identity=48.1958762886598, Blast_Score=386, Evalue=1e-107,
Organism=Homo sapiens, GI156713448, Length=416, Percent_Identity=37.2596153846154, Blast_Score=256, Evalue=2e-68,
Organism=Escherichia coli, GI48994898, Length=386, Percent_Identity=47.9274611398964, Blast_Score=379, Evalue=1e-106,
Organism=Escherichia coli, GI1787970, Length=394, Percent_Identity=27.6649746192893, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1789175, Length=397, Percent_Identity=26.448362720403, Blast_Score=114, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI25143064, Length=386, Percent_Identity=48.7046632124352, Blast_Score=373, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI17533177, Length=331, Percent_Identity=34.441087613293, Blast_Score=176, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6319831, Length=384, Percent_Identity=49.21875, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI20129463, Length=385, Percent_Identity=47.2727272727273, Blast_Score=367, Evalue=1e-102,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR010240
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 43191; Mature: 43191

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKIYLDHGATTPMREEVFETMKPYLQDNFGNPSSIHSFGREVRKAVDEAREQVANAINA
CCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
QPEEIIFTSGGTESDNLAIQGVIEKIGDKGNHIITTQIEHHAVLDTCEAMEEKGYKVTYL
CCCEEEEECCCCCCCCHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHHHHCCCEEEEE
PVDKYGLVNPKDLQEAINEDTVLITIMHGNNEVGTIQPIKELAEIAKDNDVYFHTDAVQT
ECCCCCCCCHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEEHHHHH
VGNIPIDVQDLGIDMLTLSAHKFYGPKGIGALYIKKGTKLQRVVHGGAQERKIRPGTENV
HCCCCEEHHHCCEEEEEECHHHHCCCCCCCEEEEECCCHHHHHHHCCCCCCCCCCCCCCC
PGIVGLGKAIELATEELDTKGKHIQKLRDKLIEGLSKVEDSRLNGHPERRLPNNVNMSFE
CCCEECCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCEEE
YIEGESLLLNLDMKGIAASSGSACTSGSLDPSHVLMAMGLSHQTAHGSLRLTLGRDNTEE
EECCCEEEEEECCCCEECCCCCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCCHH
EIDYVIEAVPEIVNRLRDMSSIWKGKEANYSN
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MNKIYLDHGATTPMREEVFETMKPYLQDNFGNPSSIHSFGREVRKAVDEAREQVANAINA
CCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
QPEEIIFTSGGTESDNLAIQGVIEKIGDKGNHIITTQIEHHAVLDTCEAMEEKGYKVTYL
CCCEEEEECCCCCCCCHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHHHHCCCEEEEE
PVDKYGLVNPKDLQEAINEDTVLITIMHGNNEVGTIQPIKELAEIAKDNDVYFHTDAVQT
ECCCCCCCCHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEEHHHHH
VGNIPIDVQDLGIDMLTLSAHKFYGPKGIGALYIKKGTKLQRVVHGGAQERKIRPGTENV
HCCCCEEHHHCCEEEEEECHHHHCCCCCCCEEEEECCCHHHHHHHCCCCCCCCCCCCCCC
PGIVGLGKAIELATEELDTKGKHIQKLRDKLIEGLSKVEDSRLNGHPERRLPNNVNMSFE
CCCEECCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCEEE
YIEGESLLLNLDMKGIAASSGSACTSGSLDPSHVLMAMGLSHQTAHGSLRLTLGRDNTEE
EECCCEEEEEECCCCEECCCCCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCCHH
EIDYVIEAVPEIVNRLRDMSSIWKGKEANYSN
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10930739 [H]