Click here to switch to the map view.

The map label for this gene is phoB [H]

Identifier: 188584893

GI number: 188584893

Start: 266468

End: 266848

Strand: Direct

Name: phoB [H]

Synonym: Nther_0251

Alternate gene names: 188584893

Gene position: 266468-266848 (Clockwise)

Preceding gene: 188584892

Following gene: 188584894

Centisome position: 8.42

GC content: 31.5

Gene sequence:

>381_bases
ATGCCAAAAATATTAGTTATTGAAGATGAAAAAAATATAGTTATCCCTTTAAAGCTATATCTGCAAAAACAAGGTTTTGA
GGTTCTAGTAGCCACAAATGGAGTTGACGGAGTTTCTTTAGCACAAGAAGAAAAGCCGGATGTGATTTTATTAGATATTA
TATTGCCTAAAATGAATGGTTATTTAGTATGTGAAGCTTTGAAGGAAAATGAAGAGACGAAACACATTCCAATTATTTTC
ATAACAGCTAAATCAAATGAAACAGAAATTGAAAAAGCGTTAGCGATGGGTGGGGATGATTACCTTATTAAGCCTTTTAC
CCATACGAAGTTATATGAAGTTATCAAGAAATATTTAAAGGAGGAGAATAGCCATGAATAA

Upstream 100 bases:

>100_bases
TATGTGGGTTGACAGTGAACTTGGTGAAGGTAGTACATTCTATTTCACTTTAAATATGTAATTAAATGATATTTGTAAAT
TGCGAGAGGGGAGGCTTTAT

Downstream 100 bases:

>100_bases
AAAGCTATTAATTGTTGATGATGAAAAGAATATTAGAATGACTCTGAAAAACTGTTTATCTTATGAGGGCTATGAATTAG
AGATTGCATTGAATGGTGAA

Product: response regulator receiver protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 126; Mature: 125

Protein sequence:

>126_residues
MPKILVIEDEKNIVIPLKLYLQKQGFEVLVATNGVDGVSLAQEEKPDVILLDIILPKMNGYLVCEALKENEETKHIPIIF
ITAKSNETEIEKALAMGGDDYLIKPFTHTKLYEVIKKYLKEENSHE

Sequences:

>Translated_126_residues
MPKILVIEDEKNIVIPLKLYLQKQGFEVLVATNGVDGVSLAQEEKPDVILLDIILPKMNGYLVCEALKENEETKHIPIIF
ITAKSNETEIEKALAMGGDDYLIKPFTHTKLYEVIKKYLKEENSHE
>Mature_125_residues
PKILVIEDEKNIVIPLKLYLQKQGFEVLVATNGVDGVSLAQEEKPDVILLDIILPKMNGYLVCEALKENEETKHIPIIFI
TAKSNETEIEKALAMGGDDYLIKPFTHTKLYEVIKKYLKEENSHE

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786784, Length=119, Percent_Identity=36.9747899159664, Blast_Score=79, Evalue=9e-17,
Organism=Escherichia coli, GI1786599, Length=119, Percent_Identity=34.453781512605, Blast_Score=76, Evalue=7e-16,
Organism=Escherichia coli, GI1790860, Length=118, Percent_Identity=32.2033898305085, Blast_Score=76, Evalue=8e-16,
Organism=Escherichia coli, GI87082012, Length=122, Percent_Identity=30.327868852459, Blast_Score=75, Evalue=1e-15,
Organism=Escherichia coli, GI1789809, Length=120, Percent_Identity=33.3333333333333, Blast_Score=74, Evalue=4e-15,
Organism=Escherichia coli, GI1786911, Length=124, Percent_Identity=32.258064516129, Blast_Score=70, Evalue=4e-14,
Organism=Escherichia coli, GI1788550, Length=121, Percent_Identity=38.8429752066116, Blast_Score=70, Evalue=4e-14,
Organism=Escherichia coli, GI1788394, Length=120, Percent_Identity=30, Blast_Score=69, Evalue=8e-14,
Organism=Escherichia coli, GI1787229, Length=120, Percent_Identity=34.1666666666667, Blast_Score=68, Evalue=2e-13,
Organism=Escherichia coli, GI1790863, Length=110, Percent_Identity=32.7272727272727, Blast_Score=67, Evalue=3e-13,
Organism=Escherichia coli, GI2367329, Length=126, Percent_Identity=34.1269841269841, Blast_Score=67, Evalue=5e-13,
Organism=Escherichia coli, GI1787487, Length=103, Percent_Identity=33.0097087378641, Blast_Score=66, Evalue=6e-13,
Organism=Escherichia coli, GI1789402, Length=123, Percent_Identity=30.0813008130081, Blast_Score=64, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6322000, Length=119, Percent_Identity=28.5714285714286, Blast_Score=61, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR016032
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 14325; Mature: 14194

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKILVIEDEKNIVIPLKLYLQKQGFEVLVATNGVDGVSLAQEEKPDVILLDIILPKMNG
CCEEEEEECCCCEEEEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCEEEEEEEECCCCC
YLVCEALKENEETKHIPIIFITAKSNETEIEKALAMGGDDYLIKPFTHTKLYEVIKKYLK
EEEEEEECCCCCCCCEEEEEEEECCCCHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHH
EENSHE
CCCCCC
>Mature Secondary Structure 
PKILVIEDEKNIVIPLKLYLQKQGFEVLVATNGVDGVSLAQEEKPDVILLDIILPKMNG
CEEEEEECCCCEEEEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCEEEEEEEECCCCC
YLVCEALKENEETKHIPIIFITAKSNETEIEKALAMGGDDYLIKPFTHTKLYEVIKKYLK
EEEEEEECCCCCCCCEEEEEEEECCCCHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHH
EENSHE
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]