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The map label for this gene is tuf [H]

Identifier: 188584819

GI number: 188584819

Start: 200993

End: 202195

Strand: Direct

Name: tuf [H]

Synonym: Nther_0177

Alternate gene names: 188584819

Gene position: 200993-202195 (Clockwise)

Preceding gene: 188584818

Following gene: 188584820

Centisome position: 6.35

GC content: 44.89

Gene sequence:

>1203_bases
ATGGCAAAGGAGAAGTTTGAGAGGACGAAACCGCATGTCAACATAGGTACGATAGGTCACGTAGACCACGGGAAGACAAC
GTTGACAGCAGCGATGACTACTTGCTTATCCACAGCAGGTGGTGCAGAAAAGATGGAATACGACAGTATTGATCGAGCGC
CTGAAGAGAAAGAGAGAGGTATTACGATCAGTACAGCTCACGTAGAATATGAATCAGATAATCGTCACTATGCTCACGTA
GACTGTCCAGGACACGCAGACTATGTAAAGAACATGATCACCGGAGCAGCTCAGATGGACGGTTCAATCATGGTAGTAAG
TGCAGCAGATGGTCCAATGCCACAGACGAGAGAGCACATTTTGTTGAGTCGTCAGGTAGGGGTTCCTCATATAGTAGTAT
TTTTAAACAAAGCAGATATGGTAGATGACGAAGAGCTACTAGAGCTAGTAGAGATGGAAGTAAGGGACTTGTTGAACGAA
TATGACTTCCCAGGAGACGACACACCTGTAGTAACAGGTTCAGCTTTAAAAGCAATAGAGTGTGGTTGTGGAGACAGAGA
GTGTGAAAACTGTGGACCGATTTGGGAACTAGTAGATGCCATTGATGACTACATTCCAACCCCAGATCGTGATGTAGATA
AGCCATTCTTGATGCCAGTAGAGGACGTATTCAGCATAACAGGTAGAGGTACAGTAGCGACAGGAAGAGTAGAGCGAGGA
AATATCAATGTACAAGACGAAGTAGAAATTGTAGGTATGGCAGATCGTCCTAAGAAGACAGTAGTCACAGGGGTAGAGAT
GTTCAGAAAGTCAATGGACTATGGTGAAGCAGGAGACAACATTGGAGCCTTGCTACGAGGAGTTGACCGAGAAGAGATCG
AAAGAGGTCAAGTTCTAGCCAAGCCAGGCAGCATCAATCCTCATACCAAGTTTAAAGCAGAGGTATATGTACTGAAGAAA
GAAGAGGGAGGTCGTCATACTCCATTCTTCCAAGGATACAGACCTCAGTTCTACTTCAGAACAACAGACGTAACCGGGGT
TATTGAGTTACCAGAAGGAGTAGAGATGGTAATGCCTGGTGACAACGTACAGATGGAAATCAACTTGATTACGCCTATAG
CTATAGAAGAAGGATTGAGATTCGCTATTCGTGAAGGTGGCAAAACAGTAGGTGCTGGTGTTGTTACTGGCATCATTGAG
TAG

Upstream 100 bases:

>100_bases
AAAATGTTTTGCATTAAACTGGAAGAAATAAGTGAAAGCTAGTAAAAGATATGGAAGTGAATAAAAGTGAATAAAAAATT
TAAGTAAGGAGGAGTAAGCC

Downstream 100 bases:

>100_bases
GGAGTAGCATAGCCAGGGCTTAGTCCCTGGCTTTTTTGATACATTTTTATATATAATTTAAGATGTTGACATTGACTATA
TGTTGTGATAGATTTTTAGA

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MAKEKFERTKPHVNIGTIGHVDHGKTTLTAAMTTCLSTAGGAEKMEYDSIDRAPEEKERGITISTAHVEYESDNRHYAHV
DCPGHADYVKNMITGAAQMDGSIMVVSAADGPMPQTREHILLSRQVGVPHIVVFLNKADMVDDEELLELVEMEVRDLLNE
YDFPGDDTPVVTGSALKAIECGCGDRECENCGPIWELVDAIDDYIPTPDRDVDKPFLMPVEDVFSITGRGTVATGRVERG
NINVQDEVEIVGMADRPKKTVVTGVEMFRKSMDYGEAGDNIGALLRGVDREEIERGQVLAKPGSINPHTKFKAEVYVLKK
EEGGRHTPFFQGYRPQFYFRTTDVTGVIELPEGVEMVMPGDNVQMEINLITPIAIEEGLRFAIREGGKTVGAGVVTGIIE

Sequences:

>Translated_400_residues
MAKEKFERTKPHVNIGTIGHVDHGKTTLTAAMTTCLSTAGGAEKMEYDSIDRAPEEKERGITISTAHVEYESDNRHYAHV
DCPGHADYVKNMITGAAQMDGSIMVVSAADGPMPQTREHILLSRQVGVPHIVVFLNKADMVDDEELLELVEMEVRDLLNE
YDFPGDDTPVVTGSALKAIECGCGDRECENCGPIWELVDAIDDYIPTPDRDVDKPFLMPVEDVFSITGRGTVATGRVERG
NINVQDEVEIVGMADRPKKTVVTGVEMFRKSMDYGEAGDNIGALLRGVDREEIERGQVLAKPGSINPHTKFKAEVYVLKK
EEGGRHTPFFQGYRPQFYFRTTDVTGVIELPEGVEMVMPGDNVQMEINLITPIAIEEGLRFAIREGGKTVGAGVVTGIIE
>Mature_399_residues
AKEKFERTKPHVNIGTIGHVDHGKTTLTAAMTTCLSTAGGAEKMEYDSIDRAPEEKERGITISTAHVEYESDNRHYAHVD
CPGHADYVKNMITGAAQMDGSIMVVSAADGPMPQTREHILLSRQVGVPHIVVFLNKADMVDDEELLELVEMEVRDLLNEY
DFPGDDTPVVTGSALKAIECGCGDRECENCGPIWELVDAIDDYIPTPDRDVDKPFLMPVEDVFSITGRGTVATGRVERGN
INVQDEVEIVGMADRPKKTVVTGVEMFRKSMDYGEAGDNIGALLRGVDREEIERGQVLAKPGSINPHTKFKAEVYVLKKE
EGGRHTPFFQGYRPQFYFRTTDVTGVIELPEGVEMVMPGDNVQMEINLITPIAIEEGLRFAIREGGKTVGAGVVTGIIE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=399, Percent_Identity=55.6390977443609, Blast_Score=464, Evalue=1e-131,
Organism=Homo sapiens, GI223555963, Length=445, Percent_Identity=30.561797752809, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI5729864, Length=445, Percent_Identity=30.561797752809, Blast_Score=171, Evalue=2e-42,
Organism=Homo sapiens, GI4503475, Length=447, Percent_Identity=27.5167785234899, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI4503471, Length=452, Percent_Identity=27.6548672566372, Blast_Score=143, Evalue=2e-34,
Organism=Homo sapiens, GI54607086, Length=349, Percent_Identity=30.0859598853868, Blast_Score=129, Evalue=6e-30,
Organism=Homo sapiens, GI46094014, Length=324, Percent_Identity=28.7037037037037, Blast_Score=119, Evalue=7e-27,
Organism=Homo sapiens, GI194018522, Length=339, Percent_Identity=28.3185840707965, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI194097354, Length=339, Percent_Identity=28.3185840707965, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI194018520, Length=339, Percent_Identity=28.3185840707965, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI94966754, Length=138, Percent_Identity=35.5072463768116, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI157426893, Length=256, Percent_Identity=25.390625, Blast_Score=66, Evalue=6e-11,
Organism=Homo sapiens, GI82546879, Length=431, Percent_Identity=22.737819025522, Blast_Score=65, Evalue=7e-11,
Organism=Escherichia coli, GI1790412, Length=399, Percent_Identity=73.9348370927318, Blast_Score=578, Evalue=1e-166,
Organism=Escherichia coli, GI1789737, Length=399, Percent_Identity=73.9348370927318, Blast_Score=577, Evalue=1e-166,
Organism=Escherichia coli, GI2367247, Length=376, Percent_Identity=28.7234042553192, Blast_Score=134, Evalue=8e-33,
Organism=Escherichia coli, GI48994988, Length=277, Percent_Identity=31.4079422382672, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1789108, Length=262, Percent_Identity=28.6259541984733, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1790835, Length=149, Percent_Identity=31.5436241610738, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1788922, Length=147, Percent_Identity=31.9727891156463, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=402, Percent_Identity=50, Blast_Score=386, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI25141371, Length=391, Percent_Identity=41.4322250639386, Blast_Score=318, Evalue=3e-87,
Organism=Caenorhabditis elegans, GI17552884, Length=459, Percent_Identity=28.322440087146, Blast_Score=147, Evalue=8e-36,
Organism=Caenorhabditis elegans, GI17569207, Length=459, Percent_Identity=28.322440087146, Blast_Score=147, Evalue=8e-36,
Organism=Caenorhabditis elegans, GI32566629, Length=454, Percent_Identity=27.0925110132159, Blast_Score=131, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI115532067, Length=444, Percent_Identity=26.5765765765766, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI115532065, Length=441, Percent_Identity=26.530612244898, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI32566303, Length=444, Percent_Identity=27.027027027027, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17506081, Length=303, Percent_Identity=27.0627062706271, Blast_Score=104, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI32566301, Length=159, Percent_Identity=35.8490566037736, Blast_Score=88, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17557151, Length=237, Percent_Identity=25.3164556962025, Blast_Score=78, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6324761, Length=399, Percent_Identity=64.4110275689223, Blast_Score=535, Evalue=1e-153,
Organism=Saccharomyces cerevisiae, GI6325337, Length=450, Percent_Identity=29.3333333333333, Blast_Score=150, Evalue=4e-37,
Organism=Saccharomyces cerevisiae, GI6319594, Length=450, Percent_Identity=29.3333333333333, Blast_Score=150, Evalue=4e-37,
Organism=Saccharomyces cerevisiae, GI6322937, Length=360, Percent_Identity=23.6111111111111, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6320377, Length=343, Percent_Identity=27.6967930029155, Blast_Score=94, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6320863, Length=289, Percent_Identity=25.2595155709343, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6323320, Length=243, Percent_Identity=26.7489711934156, Blast_Score=67, Evalue=3e-12,
Organism=Drosophila melanogaster, GI281363316, Length=398, Percent_Identity=54.5226130653266, Blast_Score=444, Evalue=1e-125,
Organism=Drosophila melanogaster, GI17864358, Length=398, Percent_Identity=54.5226130653266, Blast_Score=444, Evalue=1e-125,
Organism=Drosophila melanogaster, GI19921738, Length=389, Percent_Identity=47.5578406169666, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI45553807, Length=462, Percent_Identity=28.1385281385281, Blast_Score=150, Evalue=1e-36,
Organism=Drosophila melanogaster, GI45553816, Length=462, Percent_Identity=28.1385281385281, Blast_Score=150, Evalue=1e-36,
Organism=Drosophila melanogaster, GI24651721, Length=462, Percent_Identity=28.1385281385281, Blast_Score=150, Evalue=1e-36,
Organism=Drosophila melanogaster, GI17864154, Length=462, Percent_Identity=28.1385281385281, Blast_Score=150, Evalue=1e-36,
Organism=Drosophila melanogaster, GI24652838, Length=463, Percent_Identity=28.5097192224622, Blast_Score=145, Evalue=4e-35,
Organism=Drosophila melanogaster, GI17137572, Length=463, Percent_Identity=28.5097192224622, Blast_Score=145, Evalue=4e-35,
Organism=Drosophila melanogaster, GI19922690, Length=309, Percent_Identity=30.0970873786408, Blast_Score=138, Evalue=6e-33,
Organism=Drosophila melanogaster, GI45550900, Length=451, Percent_Identity=27.7161862527716, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI17137380, Length=348, Percent_Identity=28.735632183908, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI28572034, Length=165, Percent_Identity=35.1515151515151, Blast_Score=65, Evalue=9e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 43954; Mature: 43823

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEKFERTKPHVNIGTIGHVDHGKTTLTAAMTTCLSTAGGAEKMEYDSIDRAPEEKERG
CCCHHHHHCCCCEEECEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHCC
ITISTAHVEYESDNRHYAHVDCPGHADYVKNMITGAAQMDGSIMVVSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHE
LLSRQVGVPHIVVFLNKADMVDDEELLELVEMEVRDLLNEYDFPGDDTPVVTGSALKAIE
EEECCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCHHHEEE
CGCGDRECENCGPIWELVDAIDDYIPTPDRDVDKPFLMPVEDVFSITGRGTVATGRVERG
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCEEEECEEECC
NINVQDEVEIVGMADRPKKTVVTGVEMFRKSMDYGEAGDNIGALLRGVDREEIERGQVLA
CCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCHHHHCCCCEEE
KPGSINPHTKFKAEVYVLKKEEGGRHTPFFQGYRPQFYFRTTDVTGVIELPEGVEMVMPG
CCCCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCEEEECC
DNVQMEINLITPIAIEEGLRFAIREGGKTVGAGVVTGIIE
CCEEEEEEEECCHHHHHCCHHHHHCCCCEECCHHHHEECC
>Mature Secondary Structure 
AKEKFERTKPHVNIGTIGHVDHGKTTLTAAMTTCLSTAGGAEKMEYDSIDRAPEEKERG
CCHHHHHCCCCEEECEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHCC
ITISTAHVEYESDNRHYAHVDCPGHADYVKNMITGAAQMDGSIMVVSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHE
LLSRQVGVPHIVVFLNKADMVDDEELLELVEMEVRDLLNEYDFPGDDTPVVTGSALKAIE
EEECCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCHHHEEE
CGCGDRECENCGPIWELVDAIDDYIPTPDRDVDKPFLMPVEDVFSITGRGTVATGRVERG
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCEEEECEEECC
NINVQDEVEIVGMADRPKKTVVTGVEMFRKSMDYGEAGDNIGALLRGVDREEIERGQVLA
CCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCHHHHCCCCEEE
KPGSINPHTKFKAEVYVLKKEEGGRHTPFFQGYRPQFYFRTTDVTGVIELPEGVEMVMPG
CCCCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCEEEECC
DNVQMEINLITPIAIEEGLRFAIREGGKTVGAGVVTGIIE
CCEEEEEEEECCHHHHHCCHHHHHCCCCEECCHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA