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The map label for this gene is sigH [H]

Identifier: 188584818

GI number: 188584818

Start: 199476

End: 200117

Strand: Direct

Name: sigH [H]

Synonym: Nther_0176

Alternate gene names: 188584818

Gene position: 199476-200117 (Clockwise)

Preceding gene: 188584817

Following gene: 188584819

Centisome position: 6.3

GC content: 36.76

Gene sequence:

>642_bases
GTGAGTCAAAGTGCCCAAGATATTTTTGAAAAGTATGATCTCAATAGTGACGAAGAAGTTGCTAAAAGAGCCCGCGAGGG
ATGTCATGAGGCTCTTGAATTTTTGATTGGGAAATATAAAAATTTTGTTAGAGCTAAGGCGAGGTCATACTTTTTAATAG
GAGCTGACCGAGAAGATATTATTCAAGAAGGTATGATTGGCCTTTATAAAGCTATCAGGGACTTTCGCGGGGACAAGCTG
TCATCTTTTAAGGCATTTGCCGAGTTATGTATTACAAGACAGATAATTACAGCAATCAAAACGGCAACCAGGCAAAAACA
CATCCCCTTAAACTCTTATGTTTCCTTAGATAAACCAATTTACGATGAGGATTCTGATAGGACTTTACTAGATGTCATTT
CAGGTGTTAGAATTACCGATCCTGAATATTTGATGGTTAATAGGGAAGAATACAATGATATCGAGCTAAAGATGACTGAA
ATCCTGAGTAGCTTAGAATGGAAGGTGTTGATGCTGTACTTGGAAGGCAAAAGTTATCAGGAAATAGCCCAGGAATTAGA
TAGGCATGTTAAATCTATCGACAATGCATTACAGAGAGTAAAAAGAAAACTAGAGAGATATCTAAATATTAGAAGTGAAT
AG

Upstream 100 bases:

>100_bases
TTCCTTCTTTAGCTTGACGGTATATTTTAATATGCGGTATAATATGTTTATGCATAGGGGGGCTTGTTTCCCGTAGAAAA
CTAAAGCGGAGGCGATTCAT

Downstream 100 bases:

>100_bases
CAACGACTCTATCAAATATCAAGTAAGGCATTTTGCAATTCTAATCAGCTAAAATAAAATCATAAAACTTTTTAACAATA
AGGGTTGACTTTTGCAGGGA

Product: RNA polymerase factor sigma-70

Products: NA

Alternate protein names: Sigma-30 [H]

Number of amino acids: Translated: 213; Mature: 212

Protein sequence:

>213_residues
MSQSAQDIFEKYDLNSDEEVAKRAREGCHEALEFLIGKYKNFVRAKARSYFLIGADREDIIQEGMIGLYKAIRDFRGDKL
SSFKAFAELCITRQIITAIKTATRQKHIPLNSYVSLDKPIYDEDSDRTLLDVISGVRITDPEYLMVNREEYNDIELKMTE
ILSSLEWKVLMLYLEGKSYQEIAQELDRHVKSIDNALQRVKRKLERYLNIRSE

Sequences:

>Translated_213_residues
MSQSAQDIFEKYDLNSDEEVAKRAREGCHEALEFLIGKYKNFVRAKARSYFLIGADREDIIQEGMIGLYKAIRDFRGDKL
SSFKAFAELCITRQIITAIKTATRQKHIPLNSYVSLDKPIYDEDSDRTLLDVISGVRITDPEYLMVNREEYNDIELKMTE
ILSSLEWKVLMLYLEGKSYQEIAQELDRHVKSIDNALQRVKRKLERYLNIRSE
>Mature_212_residues
SQSAQDIFEKYDLNSDEEVAKRAREGCHEALEFLIGKYKNFVRAKARSYFLIGADREDIIQEGMIGLYKAIRDFRGDKLS
SFKAFAELCITRQIITAIKTATRQKHIPLNSYVSLDKPIYDEDSDRTLLDVISGVRITDPEYLMVNREEYNDIELKMTEI
LSSLEWKVLMLYLEGKSYQEIAQELDRHVKSIDNALQRVKRKLERYLNIRSE

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in the transition to post-exponential phase in the beginning of sporulation [H]

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

None

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014218
- InterPro:   IPR016371
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 24916; Mature: 24785

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS00622 HTH_LUXR_1 ; PS00715 SIGMA70_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQSAQDIFEKYDLNSDEEVAKRAREGCHEALEFLIGKYKNFVRAKARSYFLIGADREDI
CCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCHHHH
IQEGMIGLYKAIRDFRGDKLSSFKAFAELCITRQIITAIKTATRQKHIPLNSYVSLDKPI
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCC
YDEDSDRTLLDVISGVRITDPEYLMVNREEYNDIELKMTEILSSLEWKVLMLYLEGKSYQ
CCCCCCHHHHHHHCCCEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHEEEECCCCHH
EIAQELDRHVKSIDNALQRVKRKLERYLNIRSE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SQSAQDIFEKYDLNSDEEVAKRAREGCHEALEFLIGKYKNFVRAKARSYFLIGADREDI
CCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCHHHH
IQEGMIGLYKAIRDFRGDKLSSFKAFAELCITRQIITAIKTATRQKHIPLNSYVSLDKPI
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCC
YDEDSDRTLLDVISGVRITDPEYLMVNREEYNDIELKMTEILSSLEWKVLMLYLEGKSYQ
CCCCCCHHHHHHHCCCEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHEEEECCCCHH
EIAQELDRHVKSIDNALQRVKRKLERYLNIRSE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3277943; 6322121 [H]