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The map label for this gene is gltX

Identifier: 188584810

GI number: 188584810

Start: 191302

End: 192768

Strand: Direct

Name: gltX

Synonym: Nther_0168

Alternate gene names: 188584810

Gene position: 191302-192768 (Clockwise)

Preceding gene: 188584809

Following gene: 188584812

Centisome position: 6.04

GC content: 37.7

Gene sequence:

>1467_bases
TTGACTAATAAAGCGAGGTTGCGATTTGCTCCAAGCCCTACTGGACAGATCCATATCGGCAACATTCGTACCGCATTATT
TAACTGGTTGTACTCTAGACATATTGATGGAGAATTTATATTAAGAGTAGAAGATACTGATATGAACAGATCAGTTGAAG
AATACGAACAGATAATCTTTAGAGCGTTAAGTTGGCTGGGATTAGATTGGGATGAAGGCCCCCAAAAAGGTGGAGATTTC
GGTCCCTACAGACAGAGTGAGAGAAAAGATATTTATCACAAATATGCCAATAAATTATTAGAAGCTGGAAAGGCTTATTA
TTGCTACTGTACCGAAGAAGAGCTGGAAGAGATGAGAGAAGCTCAGAGGGCTAGAGGTGAAATGCCTAGATATAGCGGCA
AATGTGCCGATCTCAGCTCCGAGGAACGAGCAGAATTAGAAAATGAAGGGCGTAAGTCAGTGATTAGATTTAAAGTTCCA
GAAAATCAGACTATTCGTGTAAACGATCTGGTAAAAGGTGAAGTAGACTTTGAAAGTGATGGGATCGGAGATTTTATATT
AATAAAAAGTGATGACATGGCTTCATATAATTTTGCATGTGTAGTTGATGATTACTTAATGAACATTACCCATGTTTTAA
GGGGAGAAGATCACCTTTCCAATACTCCCAAACAAGTAATGATTTATGAGGCTCTGGGCTTTGAGACTCCTGAATTTGGA
CATTTATCTTTGATTTTAGGTCCTGATAAAGCAAAACTAAGTAAACGTCATGGTGACACCTTTATAGGGGAATATCGAGA
AAAAGGTTATCTACCTGAAGCAATGGTAAACTTTTTAGCTTTATTAGGTTGGTCTCCGCCTGGAGAAGATGAACTTTTTA
CCCAAGATGAACTAATTAGATTATTTGATATAGGTAGAGTTTCGAAAAGTGCCGCAGTCTTTGATGTGGATAAATTAAAT
TGGATGAACTCACACTATATTAAAGAGGCGGATACAGAAAGATTGCTTAACCTTTCTAAAGAATATTTAATTAATTCCGA
CCTAGTGTCGCAAGAACAGTTAGAGCACGATTGGGAATGGTTCGTTTCTGCTGTGGATTTAGTCAAGGAAAAAATTGATA
TGCTATCGGAATTACCTCCACAGTTAAAAATCTTTTATGGAGACGATGTAGACCTTAAAGGTGAGGAAGTAGAATTTTTA
GAGAAGGACCACGTTCCTGAATTACTTTCAGAAGTTATTAATCAATTTAATGATTTAGACTCCTTTGAGGATCCTAAAGC
TATCAAGAAAGCAGTTAATAAAGCCGGTAAGCAGGTTGGTGTCAAAGGTAAGCAGCTTTTTATGCCTGTAAGAATTGCAG
TGAGTGGACAAATGCACGGCCCTGAGCTTGATCAGCTAATTTCTTTATTGGGCCGAGAACGAGCGATTAATAGAGTGAAT
AGCACTCTTTCACAAATTCAAAGTTAA

Upstream 100 bases:

>100_bases
TTTTAAATTGATGGCTGCTGTATTAAAGTTGAATGTTGATAACTGAAATTTCAATTTCAAAACTAAACAGCGCCAAATTG
AAAAAGAGGGGGAATACCAA

Downstream 100 bases:

>100_bases
AATATTGACAAGAGGTATGTAATAGTTTATATTCATAAATAATAATTATTATTGACATAAAAACGAACTGAATCTAAGAT
AATTTTATGCGGAGGAATCA

Product: glutamate--tRNA(Gln) ligase, glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 488; Mature: 487

Protein sequence:

>488_residues
MTNKARLRFAPSPTGQIHIGNIRTALFNWLYSRHIDGEFILRVEDTDMNRSVEEYEQIIFRALSWLGLDWDEGPQKGGDF
GPYRQSERKDIYHKYANKLLEAGKAYYCYCTEEELEEMREAQRARGEMPRYSGKCADLSSEERAELENEGRKSVIRFKVP
ENQTIRVNDLVKGEVDFESDGIGDFILIKSDDMASYNFACVVDDYLMNITHVLRGEDHLSNTPKQVMIYEALGFETPEFG
HLSLILGPDKAKLSKRHGDTFIGEYREKGYLPEAMVNFLALLGWSPPGEDELFTQDELIRLFDIGRVSKSAAVFDVDKLN
WMNSHYIKEADTERLLNLSKEYLINSDLVSQEQLEHDWEWFVSAVDLVKEKIDMLSELPPQLKIFYGDDVDLKGEEVEFL
EKDHVPELLSEVINQFNDLDSFEDPKAIKKAVNKAGKQVGVKGKQLFMPVRIAVSGQMHGPELDQLISLLGRERAINRVN
STLSQIQS

Sequences:

>Translated_488_residues
MTNKARLRFAPSPTGQIHIGNIRTALFNWLYSRHIDGEFILRVEDTDMNRSVEEYEQIIFRALSWLGLDWDEGPQKGGDF
GPYRQSERKDIYHKYANKLLEAGKAYYCYCTEEELEEMREAQRARGEMPRYSGKCADLSSEERAELENEGRKSVIRFKVP
ENQTIRVNDLVKGEVDFESDGIGDFILIKSDDMASYNFACVVDDYLMNITHVLRGEDHLSNTPKQVMIYEALGFETPEFG
HLSLILGPDKAKLSKRHGDTFIGEYREKGYLPEAMVNFLALLGWSPPGEDELFTQDELIRLFDIGRVSKSAAVFDVDKLN
WMNSHYIKEADTERLLNLSKEYLINSDLVSQEQLEHDWEWFVSAVDLVKEKIDMLSELPPQLKIFYGDDVDLKGEEVEFL
EKDHVPELLSEVINQFNDLDSFEDPKAIKKAVNKAGKQVGVKGKQLFMPVRIAVSGQMHGPELDQLISLLGRERAINRVN
STLSQIQS
>Mature_487_residues
TNKARLRFAPSPTGQIHIGNIRTALFNWLYSRHIDGEFILRVEDTDMNRSVEEYEQIIFRALSWLGLDWDEGPQKGGDFG
PYRQSERKDIYHKYANKLLEAGKAYYCYCTEEELEEMREAQRARGEMPRYSGKCADLSSEERAELENEGRKSVIRFKVPE
NQTIRVNDLVKGEVDFESDGIGDFILIKSDDMASYNFACVVDDYLMNITHVLRGEDHLSNTPKQVMIYEALGFETPEFGH
LSLILGPDKAKLSKRHGDTFIGEYREKGYLPEAMVNFLALLGWSPPGEDELFTQDELIRLFDIGRVSKSAAVFDVDKLNW
MNSHYIKEADTERLLNLSKEYLINSDLVSQEQLEHDWEWFVSAVDLVKEKIDMLSELPPQLKIFYGDDVDLKGEEVEFLE
KDHVPELLSEVINQFNDLDSFEDPKAIKKAVNKAGKQVGVKGKQLFMPVRIAVSGQMHGPELDQLISLLGRERAINRVNS
TLSQIQS

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=517, Percent_Identity=31.1411992263056, Blast_Score=252, Evalue=5e-67,
Organism=Homo sapiens, GI62241042, Length=267, Percent_Identity=26.5917602996255, Blast_Score=81, Evalue=3e-15,
Organism=Escherichia coli, GI1788743, Length=489, Percent_Identity=39.2638036809816, Blast_Score=354, Evalue=7e-99,
Organism=Escherichia coli, GI226510925, Length=250, Percent_Identity=32.8, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI1786895, Length=212, Percent_Identity=27.8301886792453, Blast_Score=62, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI71993394, Length=485, Percent_Identity=34.020618556701, Blast_Score=277, Evalue=1e-74,
Organism=Caenorhabditis elegans, GI17511007, Length=276, Percent_Identity=24.2753623188406, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6324540, Length=514, Percent_Identity=34.0466926070039, Blast_Score=276, Evalue=7e-75,
Organism=Saccharomyces cerevisiae, GI6324742, Length=314, Percent_Identity=26.4331210191083, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6321192, Length=213, Percent_Identity=25.8215962441315, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24665321, Length=501, Percent_Identity=36.3273453093812, Blast_Score=289, Evalue=3e-78,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_NATTJ (B2A4B3)

Other databases:

- EMBL:   CP001034
- RefSeq:   YP_001916355.1
- ProteinModelPortal:   B2A4B3
- SMR:   B2A4B3
- GeneID:   6316550
- GenomeReviews:   CP001034_GR
- KEGG:   nth:Nther_0168
- HOGENOM:   HBG628189
- OMA:   WINNQYV
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 55985; Mature: 55854

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 255-255

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNKARLRFAPSPTGQIHIGNIRTALFNWLYSRHIDGEFILRVEDTDMNRSVEEYEQIIF
CCCCCEEEECCCCCCEEEECHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHH
RALSWLGLDWDEGPQKGGDFGPYRQSERKDIYHKYANKLLEAGKAYYCYCTEEELEEMRE
HHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHH
AQRARGEMPRYSGKCADLSSEERAELENEGRKSVIRFKVPENQTIRVNDLVKGEVDFESD
HHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCCEEEEECCCCCEEEEECEEECCCCCCCC
GIGDFILIKSDDMASYNFACVVDDYLMNITHVLRGEDHLSNTPKQVMIYEALGFETPEFG
CCCCEEEEECCCCCCCEEEEEHHHHHHHHHHHHCCCHHHCCCCCCEEEEHHHCCCCCCCC
HLSLILGPDKAKLSKRHGDTFIGEYREKGYLPEAMVNFLALLGWSPPGEDELFTQDELIR
CEEEEECCCHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
LFDIGRVSKSAAVFDVDKLNWMNSHYIKEADTERLLNLSKEYLINSDLVSQEQLEHDWEW
HHHHCCCCCCCEEEEHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
FVSAVDLVKEKIDMLSELPPQLKIFYGDDVDLKGEEVEFLEKDHVPELLSEVINQFNDLD
HHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHCCC
SFEDPKAIKKAVNKAGKQVGVKGKQLFMPVRIAVSGQMHGPELDQLISLLGRERAINRVN
CCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHH
STLSQIQS
HHHHHHCC
>Mature Secondary Structure 
TNKARLRFAPSPTGQIHIGNIRTALFNWLYSRHIDGEFILRVEDTDMNRSVEEYEQIIF
CCCCEEEECCCCCCEEEECHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHH
RALSWLGLDWDEGPQKGGDFGPYRQSERKDIYHKYANKLLEAGKAYYCYCTEEELEEMRE
HHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHH
AQRARGEMPRYSGKCADLSSEERAELENEGRKSVIRFKVPENQTIRVNDLVKGEVDFESD
HHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCCEEEEECCCCCEEEEECEEECCCCCCCC
GIGDFILIKSDDMASYNFACVVDDYLMNITHVLRGEDHLSNTPKQVMIYEALGFETPEFG
CCCCEEEEECCCCCCCEEEEEHHHHHHHHHHHHCCCHHHCCCCCCEEEEHHHCCCCCCCC
HLSLILGPDKAKLSKRHGDTFIGEYREKGYLPEAMVNFLALLGWSPPGEDELFTQDELIR
CEEEEECCCHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
LFDIGRVSKSAAVFDVDKLNWMNSHYIKEADTERLLNLSKEYLINSDLVSQEQLEHDWEW
HHHHCCCCCCCEEEEHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
FVSAVDLVKEKIDMLSELPPQLKIFYGDDVDLKGEEVEFLEKDHVPELLSEVINQFNDLD
HHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHCCC
SFEDPKAIKKAVNKAGKQVGVKGKQLFMPVRIAVSGQMHGPELDQLISLLGRERAINRVN
CCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHH
STLSQIQS
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA