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The map label for this gene is ispF

Identifier: 188584809

GI number: 188584809

Start: 190635

End: 191111

Strand: Direct

Name: ispF

Synonym: Nther_0167

Alternate gene names: 188584809

Gene position: 190635-191111 (Clockwise)

Preceding gene: 188584808

Following gene: 188584810

Centisome position: 6.02

GC content: 36.48

Gene sequence:

>477_bases
ATGAGAATAGGACATGGAGTAGATGTACATAAATTGGTTAAAGGTGAAGACTTAGTTTTAGGTGGAGTTCAGATTCCATC
AGACCTTGGCTTGAAAGGTCATTCCGATGCAGATGTGTTAATTCATGCAATTATGGATGCTATGTTAGGGTCACTGGCTC
TAGGTGATATCGGTAAACACTTTCCAGATGACGACCCTCGCTATCATCAGATAGATAGCAAAGTATTACTTAAAAAAACT
TATCAATTAATTACTTCTTATGGCTATCAGTTGATAAATTTAGATAGTACAATTATGGCAGAAAAACCAAAACTGGCACC
ATATATTGACGAAATGAGAATTACTATTAGTGATGTTTTAGATCTTTACCCTAGTCAGATTGGAATTAAAGCCACTACCT
TTGAAAAATTGGGCTTCGTGGGTTCGGGTCAAGGTATTATGGCACAAGCAGTGGTGTTAATGGAAAGACTGTATTAA

Upstream 100 bases:

>100_bases
AGGTGTCTGTAGTTCAAGGAAGTTTTGAAAACATTAAAGTAACAACTCCAGAAGACCTGTTTATGGCAGAAGAGATATTG
AAAAAACGGGAGGAGCAAAT

Downstream 100 bases:

>100_bases
AGCCTGTTCATGGCTATTGTAGATAAATAGTAGCTTTGATATAATTTACAAGAACTGATTTGAATTGATAACACACATTA
ACATAGAAGTTTTTAAATTG

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS

Number of amino acids: Translated: 158; Mature: 158

Protein sequence:

>158_residues
MRIGHGVDVHKLVKGEDLVLGGVQIPSDLGLKGHSDADVLIHAIMDAMLGSLALGDIGKHFPDDDPRYHQIDSKVLLKKT
YQLITSYGYQLINLDSTIMAEKPKLAPYIDEMRITISDVLDLYPSQIGIKATTFEKLGFVGSGQGIMAQAVVLMERLY

Sequences:

>Translated_158_residues
MRIGHGVDVHKLVKGEDLVLGGVQIPSDLGLKGHSDADVLIHAIMDAMLGSLALGDIGKHFPDDDPRYHQIDSKVLLKKT
YQLITSYGYQLINLDSTIMAEKPKLAPYIDEMRITISDVLDLYPSQIGIKATTFEKLGFVGSGQGIMAQAVVLMERLY
>Mature_158_residues
MRIGHGVDVHKLVKGEDLVLGGVQIPSDLGLKGHSDADVLIHAIMDAMLGSLALGDIGKHFPDDDPRYHQIDSKVLLKKT
YQLITSYGYQLINLDSTIMAEKPKLAPYIDEMRITISDVLDLYPSQIGIKATTFEKLGFVGSGQGIMAQAVVLMERLY

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=154, Percent_Identity=49.3506493506493, Blast_Score=152, Evalue=9e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPF_NATTJ (B2A4B2)

Other databases:

- EMBL:   CP001034
- RefSeq:   YP_001916354.1
- ProteinModelPortal:   B2A4B2
- SMR:   B2A4B2
- GeneID:   6316549
- GenomeReviews:   CP001034_GR
- KEGG:   nth:Nther_0167
- HOGENOM:   HBG630070
- OMA:   HKFGGEG
- HAMAP:   MF_00107
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF02542 YgbB; SSF69765 YgbB_synth

EC number: =4.6.1.12

Molecular weight: Translated: 17337; Mature: 17337

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGHGVDVHKLVKGEDLVLGGVQIPSDLGLKGHSDADVLIHAIMDAMLGSLALGDIGKH
CCCCCCCCHHHHHCCCCEEEECEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
FPDDDPRYHQIDSKVLLKKTYQLITSYGYQLINLDSTIMAEKPKLAPYIDEMRITISDVL
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHCCCCCCCCCHHHHHHHHHHHH
DLYPSQIGIKATTFEKLGFVGSGQGIMAQAVVLMERLY
HHCHHHCCCEEEHHHHHCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MRIGHGVDVHKLVKGEDLVLGGVQIPSDLGLKGHSDADVLIHAIMDAMLGSLALGDIGKH
CCCCCCCCHHHHHCCCCEEEECEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
FPDDDPRYHQIDSKVLLKKTYQLITSYGYQLINLDSTIMAEKPKLAPYIDEMRITISDVL
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHCCCCCCCCCHHHHHHHHHHHH
DLYPSQIGIKATTFEKLGFVGSGQGIMAQAVVLMERLY
HHCHHHCCCEEEHHHHHCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA