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The map label for this gene is nfo

Identifier: 188584796

GI number: 188584796

Start: 178376

End: 179230

Strand: Direct

Name: nfo

Synonym: Nther_0154

Alternate gene names: 188584796

Gene position: 178376-179230 (Clockwise)

Preceding gene: 188584795

Following gene: 188584797

Centisome position: 5.63

GC content: 39.3

Gene sequence:

>855_bases
ATGAGATTGGGTTTTCACATGCCCCTTTCGGGTGGTGTGGGTAAACAATTAAATCGTGCTAGTGAATTAGGTATGGAATG
TATACAAATTTTTTCAGGGAATCCAACTAGCTGGAAGCCAGGCAAAATCACACCAAAATCTCGAGATTTATTTATAAAAA
AACAGGAGGAATTACAGATAAAACCTTTAGTGTTTCATACTCCCTACTTGATTAACCTGGCTTCTCCTAAAAATGATATT
AGGGAAAAATCAATCTACTTATTGAATGCTGCCTTGGAAAAAGCCAAAGCTTACGATGCTCCTTATGTGGTAACTCATAT
CGGTAGTCATGTGGGTGAAGGAGTTGAAAAGGGAATTGATCTGGTGAGTTATAGTTTAGAAAAGATTATGGAGGATTGGC
CTGAGGGGGTAGAATTACTACTGGAAAACACTTCAGGAGCTGGATCTACTCTAGGTGGTAGTTTAACCGAACTTAAAAAA
ATTATAGATAAATTTTCAGGAACCCAGGTGCTCGGGTGCTGTTTTGATACTGCTCATGCTTGGGGTGCCGGTTACGACAT
TTCAAATGTCAAGGAGGTAGAGACCACTCTGGAACTGGTTAATGAACAGCTGGGCCTGGATTTGATCAAAGTTTGCCATG
CCAATGATACCAATGTCCCTTTAGGATCTACTAAAGATAGGCATCAACATATTGGTGAAGGTAATATCACAGATGAGGGA
TTCGGTGCTTTGTTAACTCATGATTCATTTACACCAAAGGCAGTTATTATGGAAACACCAAAAAATGGTACAGACTGTGA
CCAAATAAACTTACAACGATTAAAAAAGGTGGTTGGCAGGCATGAAGAAGAATGA

Upstream 100 bases:

>100_bases
AAATTTTAAAAACAATCCACCGTATCTAATGGCAGTTTTAAAAAACAAGTAAAAACTAGACAGTGAATTGCGGTTTGAAC
ATACGATAGGGGGGAAAGTT

Downstream 100 bases:

>100_bases
TTTTAAAATGACAGAACAGAATATTTATTCATATATGTCCCCCGATAATATTAAAGTGGAGGTGTTTTTATCACCTCTAT
TATTTGAGTACTTAGAAGAG

Product: apurinic endonuclease Apn1

Products: NA

Alternate protein names: Endodeoxyribonuclease IV; Endonuclease IV

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MRLGFHMPLSGGVGKQLNRASELGMECIQIFSGNPTSWKPGKITPKSRDLFIKKQEELQIKPLVFHTPYLINLASPKNDI
REKSIYLLNAALEKAKAYDAPYVVTHIGSHVGEGVEKGIDLVSYSLEKIMEDWPEGVELLLENTSGAGSTLGGSLTELKK
IIDKFSGTQVLGCCFDTAHAWGAGYDISNVKEVETTLELVNEQLGLDLIKVCHANDTNVPLGSTKDRHQHIGEGNITDEG
FGALLTHDSFTPKAVIMETPKNGTDCDQINLQRLKKVVGRHEEE

Sequences:

>Translated_284_residues
MRLGFHMPLSGGVGKQLNRASELGMECIQIFSGNPTSWKPGKITPKSRDLFIKKQEELQIKPLVFHTPYLINLASPKNDI
REKSIYLLNAALEKAKAYDAPYVVTHIGSHVGEGVEKGIDLVSYSLEKIMEDWPEGVELLLENTSGAGSTLGGSLTELKK
IIDKFSGTQVLGCCFDTAHAWGAGYDISNVKEVETTLELVNEQLGLDLIKVCHANDTNVPLGSTKDRHQHIGEGNITDEG
FGALLTHDSFTPKAVIMETPKNGTDCDQINLQRLKKVVGRHEEE
>Mature_284_residues
MRLGFHMPLSGGVGKQLNRASELGMECIQIFSGNPTSWKPGKITPKSRDLFIKKQEELQIKPLVFHTPYLINLASPKNDI
REKSIYLLNAALEKAKAYDAPYVVTHIGSHVGEGVEKGIDLVSYSLEKIMEDWPEGVELLLENTSGAGSTLGGSLTELKK
IIDKFSGTQVLGCCFDTAHAWGAGYDISNVKEVETTLELVNEQLGLDLIKVCHANDTNVPLGSTKDRHQHIGEGNITDEG
FGALLTHDSFTPKAVIMETPKNGTDCDQINLQRLKKVVGRHEEE

Specific function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by ble

COG id: COG0648

COG function: function code L; Endonuclease IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AP endonuclease 2 family

Homologues:

Organism=Escherichia coli, GI1788483, Length=264, Percent_Identity=31.4393939393939, Blast_Score=129, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17531193, Length=261, Percent_Identity=29.8850574712644, Blast_Score=126, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6322735, Length=263, Percent_Identity=28.5171102661597, Blast_Score=117, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): END4_NATTJ (B2A499)

Other databases:

- EMBL:   CP001034
- RefSeq:   YP_001916341.1
- ProteinModelPortal:   B2A499
- SMR:   B2A499
- GeneID:   6314728
- GenomeReviews:   CP001034_GR
- KEGG:   nth:Nther_0154
- HOGENOM:   HBG565018
- OMA:   QIALETM
- GO:   GO:0005622
- HAMAP:   MF_00152
- InterPro:   IPR001719
- InterPro:   IPR013022
- InterPro:   IPR012307
- Gene3D:   G3DSA:3.20.20.150
- PANTHER:   PTHR21445
- SMART:   SM00518
- TIGRFAMs:   TIGR00587

Pfam domain/function: PF01261 AP_endonuc_2; SSF51658 Xyl_isomerase-like_TIM-brl

EC number: =3.1.21.2

Molecular weight: Translated: 31181; Mature: 31181

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS00729 AP_NUCLEASE_F2_1; PS00730 AP_NUCLEASE_F2_2; PS00731 AP_NUCLEASE_F2_3; PS51432 AP_NUCLEASE_F2_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLGFHMPLSGGVGKQLNRASELGMECIQIFSGNPTSWKPGKITPKSRDLFIKKQEELQI
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCEE
KPLVFHTPYLINLASPKNDIREKSIYLLNAALEKAKAYDAPYVVTHIGSHVGEGVEKGID
CEEEEECCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
LVSYSLEKIMEDWPEGVELLLENTSGAGSTLGGSLTELKKIIDKFSGTQVLGCCFDTAHA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEHHHHHHHH
WGAGYDISNVKEVETTLELVNEQLGLDLIKVCHANDTNVPLGSTKDRHQHIGEGNITDEG
CCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCCCCCC
FGALLTHDSFTPKAVIMETPKNGTDCDQINLQRLKKVVGRHEEE
CCEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MRLGFHMPLSGGVGKQLNRASELGMECIQIFSGNPTSWKPGKITPKSRDLFIKKQEELQI
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCEE
KPLVFHTPYLINLASPKNDIREKSIYLLNAALEKAKAYDAPYVVTHIGSHVGEGVEKGID
CEEEEECCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
LVSYSLEKIMEDWPEGVELLLENTSGAGSTLGGSLTELKKIIDKFSGTQVLGCCFDTAHA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEHHHHHHHH
WGAGYDISNVKEVETTLELVNEQLGLDLIKVCHANDTNVPLGSTKDRHQHIGEGNITDEG
CCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCCCCCC
FGALLTHDSFTPKAVIMETPKNGTDCDQINLQRLKKVVGRHEEE
CCEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA