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The map label for this gene is murE [H]

Identifier: 188584682

GI number: 188584682

Start: 53936

End: 55492

Strand: Reverse

Name: murE [H]

Synonym: Nther_0040

Alternate gene names: 188584682

Gene position: 55492-53936 (Counterclockwise)

Preceding gene: 188584684

Following gene: 188584678

Centisome position: 1.75

GC content: 37.51

Gene sequence:

>1557_bases
ATGCCTGGTTCATCCGAAAACTCCCGAAAAATCAGTTTAAAGAAGTTATTACAAAACACCTCACCTGAGCTGTTTACTGA
ATTTGCCGACTTAGGCACTACTGGCACAGATATTGAAGGTGTAACTATTTCCGGGATTACCGACAACTCAAAAGAAGTAA
CTCCAGGCAAGATCTTTGTAGCTTGTCAGGGCACTAAGGTAGACGCCCACGACTATATAGAAGAGGCGATTGCAAATGGA
GCTTCAACAGTCATTCTAGAGAGAGCCGATTACATTGACAAGGTCAAGGATAGCGCCATTCCTTATTTTAAAGTAAGGAA
TGGTAGGAAGACCCTGGCAAAGTTAGCTTCCACTTTTTACGATCATCCTTCTCACAGATTAATAGTTACAGGTATTACCG
GTACCAATGGCAAAACAACTACTACAACATTAATTGATTATATTATAAACCAGCTAGGAGTAAAGTCAGGACTGATTGGA
ACTGTAAACTGTAAAATTAACGGTGAAGAATTTGATTCCAAATTAACAACACCGCCAGCTACTGAGCTATATAGTTTAAT
CGAAAAAATGGTTCAAAAGGAAGTAAAGTACCTTACAAAAGAAGTATCTTCCCATGGTTTAAAGCAGGATAGAGTTTCAG
CCATTAATTTCGATATTGCTGGAATTACAAATATTTCTTACGATCATATGGATTTTCACGATTCCTGGGATGACTATTTT
AAAAGCAAGACAAAATTTTTCCAAATGTTGTCTCCTGATAGCATTTCAGTTTTAAATGCAGATGATCCCAATGCAATCTC
CATGGGGGATAAAACCAGGAGTAAAGTGATTACCTACGGAATTTCTTCACCGGGAGCTATGATAACTGCTGAAAATGTAG
ACCTAAAACCTTTTGAAACTAGTTTTACCTATCAAATCAAAGAAGAGTTGCCTACAGTGACCAATAAACCTGTTAGGCCC
CAATCACACAATTTGAAGATCAGAATGCCAGGAAAGCACAATATTTATAATACTTTACTTGCGATTTCAGTATGTATAAT
GGCAGGGTTTGACCCGGAACAGGTCATCAAAACAGCTGAACAATTTCCGGGCCTATTTCGCCGCTTAGAAGTAATTTATA
AAAACAGCTTTACTATTATAGATGATTGCGCACATAACCCTGCCAGTATTCGTTCTGTATTTGAAACAATATCACAACTT
CCCTTTAACAGATTAGTTATTGTCTTTGCAATTCGGGGAAGTCGCGGGGATCAAATAAATCTAGAAAATAGCCAGAGACT
AGTAAAGTGGGTAAATCACTTACCAGTAAGAGAGCTAATAATTACCACCAGTAAGTCGCACGCTGCCCCAACAAATCAAG
TTCATTCATCAGAACAGACCGCTTTTTTATCACCATTGAAAACATCAGGGACACCTTTTAAATTCATTCACAATCTAGAA
GAAACAATTGAAACAGCTTTAAATGAAGTTAAACAGGGTGACTTATTAATGATCCTCGGTCCCTATGCAATTAATGAAGG
GCAAGAAATAGTCAGAAACTATCTATCAAATTCTTAA

Upstream 100 bases:

>100_bases
CATAGTGTCACCTCTATTCTACTTTTTCCCAGAGAATAATATACTATACAAGGGCAAAACTAAAGCTAAACTAATATCAC
CTTCTAAAGGAGCATCATTA

Downstream 100 bases:

>100_bases
GTTAGTGTATAACTTCTTATATTAATCTTCTAACTTTCTAAGCTCATCTTCAATATCTTTTTCCAGTTGATTATTGTGGC
AGTGATCTTTAGAATGACAC

Product: UDP-N-acetylmuramyl-tripeptide synthetase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 518; Mature: 517

Protein sequence:

>518_residues
MPGSSENSRKISLKKLLQNTSPELFTEFADLGTTGTDIEGVTISGITDNSKEVTPGKIFVACQGTKVDAHDYIEEAIANG
ASTVILERADYIDKVKDSAIPYFKVRNGRKTLAKLASTFYDHPSHRLIVTGITGTNGKTTTTTLIDYIINQLGVKSGLIG
TVNCKINGEEFDSKLTTPPATELYSLIEKMVQKEVKYLTKEVSSHGLKQDRVSAINFDIAGITNISYDHMDFHDSWDDYF
KSKTKFFQMLSPDSISVLNADDPNAISMGDKTRSKVITYGISSPGAMITAENVDLKPFETSFTYQIKEELPTVTNKPVRP
QSHNLKIRMPGKHNIYNTLLAISVCIMAGFDPEQVIKTAEQFPGLFRRLEVIYKNSFTIIDDCAHNPASIRSVFETISQL
PFNRLVIVFAIRGSRGDQINLENSQRLVKWVNHLPVRELIITTSKSHAAPTNQVHSSEQTAFLSPLKTSGTPFKFIHNLE
ETIETALNEVKQGDLLMILGPYAINEGQEIVRNYLSNS

Sequences:

>Translated_518_residues
MPGSSENSRKISLKKLLQNTSPELFTEFADLGTTGTDIEGVTISGITDNSKEVTPGKIFVACQGTKVDAHDYIEEAIANG
ASTVILERADYIDKVKDSAIPYFKVRNGRKTLAKLASTFYDHPSHRLIVTGITGTNGKTTTTTLIDYIINQLGVKSGLIG
TVNCKINGEEFDSKLTTPPATELYSLIEKMVQKEVKYLTKEVSSHGLKQDRVSAINFDIAGITNISYDHMDFHDSWDDYF
KSKTKFFQMLSPDSISVLNADDPNAISMGDKTRSKVITYGISSPGAMITAENVDLKPFETSFTYQIKEELPTVTNKPVRP
QSHNLKIRMPGKHNIYNTLLAISVCIMAGFDPEQVIKTAEQFPGLFRRLEVIYKNSFTIIDDCAHNPASIRSVFETISQL
PFNRLVIVFAIRGSRGDQINLENSQRLVKWVNHLPVRELIITTSKSHAAPTNQVHSSEQTAFLSPLKTSGTPFKFIHNLE
ETIETALNEVKQGDLLMILGPYAINEGQEIVRNYLSNS
>Mature_517_residues
PGSSENSRKISLKKLLQNTSPELFTEFADLGTTGTDIEGVTISGITDNSKEVTPGKIFVACQGTKVDAHDYIEEAIANGA
STVILERADYIDKVKDSAIPYFKVRNGRKTLAKLASTFYDHPSHRLIVTGITGTNGKTTTTTLIDYIINQLGVKSGLIGT
VNCKINGEEFDSKLTTPPATELYSLIEKMVQKEVKYLTKEVSSHGLKQDRVSAINFDIAGITNISYDHMDFHDSWDDYFK
SKTKFFQMLSPDSISVLNADDPNAISMGDKTRSKVITYGISSPGAMITAENVDLKPFETSFTYQIKEELPTVTNKPVRPQ
SHNLKIRMPGKHNIYNTLLAISVCIMAGFDPEQVIKTAEQFPGLFRRLEVIYKNSFTIIDDCAHNPASIRSVFETISQLP
FNRLVIVFAIRGSRGDQINLENSQRLVKWVNHLPVRELIITTSKSHAAPTNQVHSSEQTAFLSPLKTSGTPFKFIHNLEE
TIETALNEVKQGDLLMILGPYAINEGQEIVRNYLSNS

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=380, Percent_Identity=28.4210526315789, Blast_Score=105, Evalue=1e-23,
Organism=Escherichia coli, GI1786274, Length=444, Percent_Identity=23.6486486486486, Blast_Score=89, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 57526; Mature: 57395

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: PS00259 GASTRIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGSSENSRKISLKKLLQNTSPELFTEFADLGTTGTDIEGVTISGITDNSKEVTPGKIFV
CCCCCCCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCEEEE
ACQGTKVDAHDYIEEAIANGASTVILERADYIDKVKDSAIPYFKVRNGRKTLAKLASTFY
EEECCCCCHHHHHHHHHHCCCCEEEEEHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHH
DHPSHRLIVTGITGTNGKTTTTTLIDYIINQLGVKSGLIGTVNCKINGEEFDSKLTTPPA
CCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEECCHHHCCCCCCCCH
TELYSLIEKMVQKEVKYLTKEVSSHGLKQDRVSAINFDIAGITNISYDHMDFHDSWDDYF
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCEEEEEEEEEECCCCCCCCCCCCCHHHHH
KSKTKFFQMLSPDSISVLNADDPNAISMGDKTRSKVITYGISSPGAMITAENVDLKPFET
HHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCEEEECCCCCCCCCC
SFTYQIKEELPTVTNKPVRPQSHNLKIRMPGKHNIYNTLLAISVCIMAGFDPEQVIKTAE
CEEEEEHHHCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHH
QFPGLFRRLEVIYKNSFTIIDDCAHNPASIRSVFETISQLPFNRLVIVFAIRGSRGDQIN
HHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCEEC
LENSQRLVKWVNHLPVRELIITTSKSHAAPTNQVHSSEQTAFLSPLKTSGTPFKFIHNLE
CCHHHHHHHHHHCCCHHHHEEECCCCCCCCCHHHCCCCCHHHHCCCCCCCCHHHHHHHHH
ETIETALNEVKQGDLLMILGPYAINEGQEIVRNYLSNS
HHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHCCC
>Mature Secondary Structure 
PGSSENSRKISLKKLLQNTSPELFTEFADLGTTGTDIEGVTISGITDNSKEVTPGKIFV
CCCCCCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCEEEE
ACQGTKVDAHDYIEEAIANGASTVILERADYIDKVKDSAIPYFKVRNGRKTLAKLASTFY
EEECCCCCHHHHHHHHHHCCCCEEEEEHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHH
DHPSHRLIVTGITGTNGKTTTTTLIDYIINQLGVKSGLIGTVNCKINGEEFDSKLTTPPA
CCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEECCHHHCCCCCCCCH
TELYSLIEKMVQKEVKYLTKEVSSHGLKQDRVSAINFDIAGITNISYDHMDFHDSWDDYF
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCEEEEEEEEEECCCCCCCCCCCCCHHHHH
KSKTKFFQMLSPDSISVLNADDPNAISMGDKTRSKVITYGISSPGAMITAENVDLKPFET
HHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCEEEECCCCCCCCCC
SFTYQIKEELPTVTNKPVRPQSHNLKIRMPGKHNIYNTLLAISVCIMAGFDPEQVIKTAE
CEEEEEHHHCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHH
QFPGLFRRLEVIYKNSFTIIDDCAHNPASIRSVFETISQLPFNRLVIVFAIRGSRGDQIN
HHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCEEC
LENSQRLVKWVNHLPVRELIITTSKSHAAPTNQVHSSEQTAFLSPLKTSGTPFKFIHNLE
CCHHHHHHHHHHCCCHHHHEEECCCCCCCCCHHHCCCCCHHHHCCCCCCCCHHHHHHHHH
ETIETALNEVKQGDLLMILGPYAINEGQEIVRNYLSNS
HHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8436954; 9384377; 8289242 [H]