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The map label for this gene is pcrA [H]

Identifier: 188528249

GI number: 188528249

Start: 1469096

End: 1471141

Strand: Reverse

Name: pcrA [H]

Synonym: HPSH_07575

Alternate gene names: 188528249

Gene position: 1471141-1469096 (Counterclockwise)

Preceding gene: 188528250

Following gene: 188528248

Centisome position: 91.46

GC content: 39.88

Gene sequence:

>2046_bases
ATGGATTTTGAAAAAAGCATTTTAGACAATTTAAACGAAGCGCAAAAAATCGCCGCAAGCCACATTCAAGGGCCTTTGCT
GATTTTAGCGGGAGCTGGGAGCGGTAAGACTAAGACTTTAACGAGCCGTTTAGCGTATTTGATTGGCGCTTGTGGCGTGC
CTAGCGAGAACACTTTAACGCTCACTTTCACCAATAAAGCGAGTAAAGAAATGCAAGAAAGGGCTTTGAAATTGTTGGAA
AACCAAACCCTTATCCCCCCCTTGCTTTGCACTTTCCATCGTTTTGGTTTGCTGTTTTTAAGGCAACACATGAACCTTTT
AAAAAGAGCGTGCGATTTTTCGGTGTTAGATGGCGATGAAGTAAAAACGCTGTGCAAACAGCTTAAAATTTCAAGCTTTA
GAGCCAGTATTTCTCAGATCAAAAACGGCATGGTTGATTTAAGCATGCAAGATAGCGAATGTCATAAAGCGTATGAGCTT
TACCAAAACGCGCTTAAAAAAGACAATTTAGTGGATTTTGACGACTTGCTTTTTTTAAGCCTTAAGATTTTGCAAGATAA
TGAAACCATCGCCAAAGAAACTAGCGAGCGCTACCATTACATTATGGTAGATGAGTATCAAGACACGAACGCCTTGCAAT
TAGAATTTTTAAAAAAATTGAGTTTCACGCACCATAATTTGTGCGTGGTGGGCGATGACGATCAGAGCATTTATGGTTTT
AGGGGGGCTGATATTTCTAACATTTTAAATTTTTCCAAGCATTTTAAAGGGGTTAAGGTGGTGAAATTAGAAACCAACTA
CCGCTCCAGCGCTGAAATTTTAGCGTGCGCTAACTCCCTTATTAGCCATAACCAACACCGCCACAAAAAAACGCTTCAAA
GTTTTAAAGGCTCGCATAAAAGCGTGGTTTGTAAAGAATATTTGACGCAAAAAGAAGAGAGCTTGGATGTGGCTTATCAA
ATCAAAGCCCTTTTAAAGAAGGGCGAAAATTTAGAAAATATCGCTATATTGTATCGCTTAAACGGGCTTAGCCACAGCAT
TGAAGAGAGCTTGAACGCTTTGAATATCCCCTATAGGCTTATTGGAGCGGTAAGTTTCTATGAAAGAGCAGAGGTTAAAG
ACGCTTTGGCGTTCATGCATTTAGTGGCTAAAAAAGACGATCGCTTTTTTATCAGGCGCGTTTTAAACAAGCCCTCAAGA
GGCCTTGGCAAGATCACCCAAGAATGGATTTTTTCTCTTTTAGATGATGAGGGTTTGAATTTAGAAGAAGCGCTAAAACT
TGGGGTGTTTAAAGGCAAATTAAACCCTAAAAACGAACACGCTTTGAATAAATTTATCGCTATGATAGGGCGTTTGAGGG
AGGCTTTTGAAATTTCAGTAGAGGAGTTTTGCGCTCGGTTTTTAGAAGAAACCAACCTTTTAAAAAGCTATGAAAAAGAA
GACAATTACGAAGAAAGAGAGGGCTTTGTTAAAGAGCTTTTAGCCTTAGTGAAAGAGCATTTTAAAACTAACCCCACGCA
TTCTTTATTGGATTTTTTGAATGAAAGCGTTCTAGATACCCATAATACAGAAAACGCGCAAAAAGTGAGTTGCATGAGCG
TGCATATGAGTAAGGGGTTAGAGTTTAAGCATGTGTTTGTGATCGGGTTAGAAGAAGGGTTTTTCCCGCATAGGGGGTTC
AATCAAGAAAGCGATTTAGAAGAAGAAAGGCGCTTGGCTTATGTAGCGATCACTAGGGCTAAAGAAGGATTGCAGCTCTC
TTATGTTCAAGAGCGTTCGTATTTTGGGAGGAAAATTTCTTGCTCGCCCTCTGTGTTTTTAGAAGAAGCTAAGTTGCTCA
AGCAAGACAACCCCCCTAAACAGGATCACAAAAAAGATACGCCCATTAAAGTGGGGGATTTGATCAGGCATAAGATTTTT
GGCACCGGGAGGGTTTTAGGCGTAGAAAAAGGCTTGAGCGGTTTGTGCTTGAAAATCAATTGCGGAGGGAATGTCTATGA
TAAAATCTCAGTAAAATTTGTAGAAAAAGTGGATAATGGGTTTTGA

Upstream 100 bases:

>100_bases
AAACCTCTGATTTTTCTTCGCCTTATTTTGATTTTGAAATTGACACCAACCTTTTAAAAGAGCGTTTGAACCAAGAAAAA
AAGGGCGAGTTTTTAGAATA

Downstream 100 bases:

>100_bases
AAATTTTAATCCTTTTTTTGATCTGTTTAAACGCGCTGTTCGCCCTGGATTCAAACGCGCTTAAAACAGAGATTAAAGAA
GCTTACCTTAAAGAATACAA

Product: DNA helicase II

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 681; Mature: 681

Protein sequence:

>681_residues
MDFEKSILDNLNEAQKIAASHIQGPLLILAGAGSGKTKTLTSRLAYLIGACGVPSENTLTLTFTNKASKEMQERALKLLE
NQTLIPPLLCTFHRFGLLFLRQHMNLLKRACDFSVLDGDEVKTLCKQLKISSFRASISQIKNGMVDLSMQDSECHKAYEL
YQNALKKDNLVDFDDLLFLSLKILQDNETIAKETSERYHYIMVDEYQDTNALQLEFLKKLSFTHHNLCVVGDDDQSIYGF
RGADISNILNFSKHFKGVKVVKLETNYRSSAEILACANSLISHNQHRHKKTLQSFKGSHKSVVCKEYLTQKEESLDVAYQ
IKALLKKGENLENIAILYRLNGLSHSIEESLNALNIPYRLIGAVSFYERAEVKDALAFMHLVAKKDDRFFIRRVLNKPSR
GLGKITQEWIFSLLDDEGLNLEEALKLGVFKGKLNPKNEHALNKFIAMIGRLREAFEISVEEFCARFLEETNLLKSYEKE
DNYEEREGFVKELLALVKEHFKTNPTHSLLDFLNESVLDTHNTENAQKVSCMSVHMSKGLEFKHVFVIGLEEGFFPHRGF
NQESDLEEERRLAYVAITRAKEGLQLSYVQERSYFGRKISCSPSVFLEEAKLLKQDNPPKQDHKKDTPIKVGDLIRHKIF
GTGRVLGVEKGLSGLCLKINCGGNVYDKISVKFVEKVDNGF

Sequences:

>Translated_681_residues
MDFEKSILDNLNEAQKIAASHIQGPLLILAGAGSGKTKTLTSRLAYLIGACGVPSENTLTLTFTNKASKEMQERALKLLE
NQTLIPPLLCTFHRFGLLFLRQHMNLLKRACDFSVLDGDEVKTLCKQLKISSFRASISQIKNGMVDLSMQDSECHKAYEL
YQNALKKDNLVDFDDLLFLSLKILQDNETIAKETSERYHYIMVDEYQDTNALQLEFLKKLSFTHHNLCVVGDDDQSIYGF
RGADISNILNFSKHFKGVKVVKLETNYRSSAEILACANSLISHNQHRHKKTLQSFKGSHKSVVCKEYLTQKEESLDVAYQ
IKALLKKGENLENIAILYRLNGLSHSIEESLNALNIPYRLIGAVSFYERAEVKDALAFMHLVAKKDDRFFIRRVLNKPSR
GLGKITQEWIFSLLDDEGLNLEEALKLGVFKGKLNPKNEHALNKFIAMIGRLREAFEISVEEFCARFLEETNLLKSYEKE
DNYEEREGFVKELLALVKEHFKTNPTHSLLDFLNESVLDTHNTENAQKVSCMSVHMSKGLEFKHVFVIGLEEGFFPHRGF
NQESDLEEERRLAYVAITRAKEGLQLSYVQERSYFGRKISCSPSVFLEEAKLLKQDNPPKQDHKKDTPIKVGDLIRHKIF
GTGRVLGVEKGLSGLCLKINCGGNVYDKISVKFVEKVDNGF
>Mature_681_residues
MDFEKSILDNLNEAQKIAASHIQGPLLILAGAGSGKTKTLTSRLAYLIGACGVPSENTLTLTFTNKASKEMQERALKLLE
NQTLIPPLLCTFHRFGLLFLRQHMNLLKRACDFSVLDGDEVKTLCKQLKISSFRASISQIKNGMVDLSMQDSECHKAYEL
YQNALKKDNLVDFDDLLFLSLKILQDNETIAKETSERYHYIMVDEYQDTNALQLEFLKKLSFTHHNLCVVGDDDQSIYGF
RGADISNILNFSKHFKGVKVVKLETNYRSSAEILACANSLISHNQHRHKKTLQSFKGSHKSVVCKEYLTQKEESLDVAYQ
IKALLKKGENLENIAILYRLNGLSHSIEESLNALNIPYRLIGAVSFYERAEVKDALAFMHLVAKKDDRFFIRRVLNKPSR
GLGKITQEWIFSLLDDEGLNLEEALKLGVFKGKLNPKNEHALNKFIAMIGRLREAFEISVEEFCARFLEETNLLKSYEKE
DNYEEREGFVKELLALVKEHFKTNPTHSLLDFLNESVLDTHNTENAQKVSCMSVHMSKGLEFKHVFVIGLEEGFFPHRGF
NQESDLEEERRLAYVAITRAKEGLQLSYVQERSYFGRKISCSPSVFLEEAKLLKQDNPPKQDHKKDTPIKVGDLIRHKIF
GTGRVLGVEKGLSGLCLKINCGGNVYDKISVKFVEKVDNGF

Specific function: Essential helicase. May act as a helicase in plasmid pT181 replication [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=688, Percent_Identity=32.1220930232558, Blast_Score=343, Evalue=3e-95,
Organism=Escherichia coli, GI48994965, Length=650, Percent_Identity=34, Blast_Score=313, Evalue=2e-86,
Organism=Escherichia coli, GI1787196, Length=146, Percent_Identity=32.8767123287671, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6322369, Length=463, Percent_Identity=29.8056155507559, Blast_Score=157, Evalue=7e-39,
Organism=Saccharomyces cerevisiae, GI6324477, Length=362, Percent_Identity=25.1381215469613, Blast_Score=67, Evalue=8e-12,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 77471; Mature: 77471

Theoretical pI: Translated: 7.75; Mature: 7.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFEKSILDNLNEAQKIAASHIQGPLLILAGAGSGKTKTLTSRLAYLIGACGVPSENTLT
CCCHHHHHHCHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCEEE
LTFTNKASKEMQERALKLLENQTLIPPLLCTFHRFGLLFLRQHMNLLKRACDFSVLDGDE
EEECCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
VKTLCKQLKISSFRASISQIKNGMVDLSMQDSECHKAYELYQNALKKDNLVDFDDLLFLS
HHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
LKILQDNETIAKETSERYHYIMVDEYQDTNALQLEFLKKLSFTHHNLCVVGDDDQSIYGF
HHHHCCCCHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCEEEE
RGADISNILNFSKHFKGVKVVKLETNYRSSAEILACANSLISHNQHRHKKTLQSFKGSHK
CCCCHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCH
SVVCKEYLTQKEESLDVAYQIKALLKKGENLENIAILYRLNGLSHSIEESLNALNIPYRL
HHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHCCCCHHH
IGAVSFYERAEVKDALAFMHLVAKKDDRFFIRRVLNKPSRGLGKITQEWIFSLLDDEGLN
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCC
LEEALKLGVFKGKLNPKNEHALNKFIAMIGRLREAFEISVEEFCARFLEETNLLKSYEKE
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DNYEEREGFVKELLALVKEHFKTNPTHSLLDFLNESVLDTHNTENAQKVSCMSVHMSKGL
CCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCC
EFKHVFVIGLEEGFFPHRGFNQESDLEEERRLAYVAITRAKEGLQLSYVQERSYFGRKIS
CEEEEEEEEECCCCCCCCCCCCCCCHHHHHHEEHEEEECCCCCCEEHHHHHHHHCCCEEC
CSPSVFLEEAKLLKQDNPPKQDHKKDTPIKVGDLIRHKIFGTGRVLGVEKGLSGLCLKIN
CCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCEEEEEE
CGGNVYDKISVKFVEKVDNGF
CCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDFEKSILDNLNEAQKIAASHIQGPLLILAGAGSGKTKTLTSRLAYLIGACGVPSENTLT
CCCHHHHHHCHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCEEE
LTFTNKASKEMQERALKLLENQTLIPPLLCTFHRFGLLFLRQHMNLLKRACDFSVLDGDE
EEECCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
VKTLCKQLKISSFRASISQIKNGMVDLSMQDSECHKAYELYQNALKKDNLVDFDDLLFLS
HHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
LKILQDNETIAKETSERYHYIMVDEYQDTNALQLEFLKKLSFTHHNLCVVGDDDQSIYGF
HHHHCCCCHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCEEEE
RGADISNILNFSKHFKGVKVVKLETNYRSSAEILACANSLISHNQHRHKKTLQSFKGSHK
CCCCHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCH
SVVCKEYLTQKEESLDVAYQIKALLKKGENLENIAILYRLNGLSHSIEESLNALNIPYRL
HHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHCCCCHHH
IGAVSFYERAEVKDALAFMHLVAKKDDRFFIRRVLNKPSRGLGKITQEWIFSLLDDEGLN
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCC
LEEALKLGVFKGKLNPKNEHALNKFIAMIGRLREAFEISVEEFCARFLEETNLLKSYEKE
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DNYEEREGFVKELLALVKEHFKTNPTHSLLDFLNESVLDTHNTENAQKVSCMSVHMSKGL
CCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCC
EFKHVFVIGLEEGFFPHRGFNQESDLEEERRLAYVAITRAKEGLQLSYVQERSYFGRKIS
CEEEEEEEEECCCCCCCCCCCCCCCHHHHHHEEHEEEECCCCCCEEHHHHHHHHCCCEEC
CSPSVFLEEAKLLKQDNPPKQDHKKDTPIKVGDLIRHKIFGTGRVLGVEKGLSGLCLKIN
CCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCEEEEEE
CGGNVYDKISVKFVEKVDNGF
CCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8232203 [H]