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The map label for this gene is ilvE [H]

Identifier: 188528240

GI number: 188528240

Start: 1458281

End: 1459303

Strand: Reverse

Name: ilvE [H]

Synonym: HPSH_07520

Alternate gene names: 188528240

Gene position: 1459303-1458281 (Counterclockwise)

Preceding gene: 188528241

Following gene: 188528238

Centisome position: 90.72

GC content: 46.04

Gene sequence:

>1023_bases
ATGGCAAATTTAGAAAATTTAGACTGGAAAAATCTAGGCTTTAGTTACATTAAAACGGATTTTCGCTTCATCGCTAGTTA
TAAAAACGGCTCTTGGTCGCAAGGCGAATTGGTTAGCGAAAATGCGTTACAAATCAGCGAAGGCTCGCCGGTCTTGCACT
ACGGGCAGGCTTGTTTTGAAGGCTTGAAGGCTTACCGCTCTCAAAAGGGGAAGGCTCTACTGTTTCGCCCTTTAGAAAAC
GCCAAACGCTTGCAAACTTCATGCGAAAGACTGCTCATGCCCAAAGTGAGCGAAGAGCTGTTTTTAAGGGCATGCGCTGA
AGTGGTGAAAGCGAATCAAAAATGGCTCGCTCCTTATAAAAGTGGGGCGAGTTTGTATTTGCGCCCTTTTGTCATAGGCG
TGGGGGATAATTTGGGGGTGAAGCCGGCCAGCGAATACCTTTTTATCGTGTTTTGCGCGCCTGTGGGGGCGTATTTTAAG
GGGGGCATAGAAAAAGGAGGGGCTAGGTTTATCACTACGGCGTTTGATAGGGCCGCGCCTAAAGGCACCGGTGGGGTGAA
AGTGGGGGGGAATTATGCTGCAAGCCTGTTAGCCCACAAAATAGCCACAGAGCAAGGCTATGATGATTGCATTTATTTAG
ACCCTGCCACGCACACTAAAATTGAAGAAGTGGGGGCGGCGAATTTTTTTGGTATCACGCATGATAACGCCTTTATCACC
CCGCATTCGCCAAGCATTCTGCCAAGCATTACAAAAAAAAGCTTGATGGTTTTGGCTAAAGAATATTTGAACCTCAAAGT
AGAAGAGAGGGAAATCCTAATGGATGAGTTGGGCACGTTTAAGGAAGCTGGAGCGTGCGGGACAGCTGCGATCATTACGC
CCATTAAAGAAATCGCGCATAATGACAAGTCTTATTTTTTTGAAGCACCGGGCAACACCACTAAACAACTCTATGATTTG
CTTTTATCCATCCAACAAGGCGAACAAGAAGCCCCTAAAGATTGGATTTTTGAAGTTTGCTAA

Upstream 100 bases:

>100_bases
ATGTGCGTAGAGTCTATGCGTTTCAAATCAGTTACTTGAGGGATTTTTAACCCCTTTTTGGATACAATCACGCCTAAAAC
TATCCATTTAAAGGTGTGAA

Downstream 100 bases:

>100_bases
AAGGTTAAAATTTATAGCTGTATGCCGCATAAAATAAGGGTGAAGTCTCAAAGCGTTTGGAGCTAGAAAGCAATCTGGCG
GTGGGTAAGATTTTAAACCC

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT [H]

Number of amino acids: Translated: 340; Mature: 339

Protein sequence:

>340_residues
MANLENLDWKNLGFSYIKTDFRFIASYKNGSWSQGELVSENALQISEGSPVLHYGQACFEGLKAYRSQKGKALLFRPLEN
AKRLQTSCERLLMPKVSEELFLRACAEVVKANQKWLAPYKSGASLYLRPFVIGVGDNLGVKPASEYLFIVFCAPVGAYFK
GGIEKGGARFITTAFDRAAPKGTGGVKVGGNYAASLLAHKIATEQGYDDCIYLDPATHTKIEEVGAANFFGITHDNAFIT
PHSPSILPSITKKSLMVLAKEYLNLKVEEREILMDELGTFKEAGACGTAAIITPIKEIAHNDKSYFFEAPGNTTKQLYDL
LLSIQQGEQEAPKDWIFEVC

Sequences:

>Translated_340_residues
MANLENLDWKNLGFSYIKTDFRFIASYKNGSWSQGELVSENALQISEGSPVLHYGQACFEGLKAYRSQKGKALLFRPLEN
AKRLQTSCERLLMPKVSEELFLRACAEVVKANQKWLAPYKSGASLYLRPFVIGVGDNLGVKPASEYLFIVFCAPVGAYFK
GGIEKGGARFITTAFDRAAPKGTGGVKVGGNYAASLLAHKIATEQGYDDCIYLDPATHTKIEEVGAANFFGITHDNAFIT
PHSPSILPSITKKSLMVLAKEYLNLKVEEREILMDELGTFKEAGACGTAAIITPIKEIAHNDKSYFFEAPGNTTKQLYDL
LLSIQQGEQEAPKDWIFEVC
>Mature_339_residues
ANLENLDWKNLGFSYIKTDFRFIASYKNGSWSQGELVSENALQISEGSPVLHYGQACFEGLKAYRSQKGKALLFRPLENA
KRLQTSCERLLMPKVSEELFLRACAEVVKANQKWLAPYKSGASLYLRPFVIGVGDNLGVKPASEYLFIVFCAPVGAYFKG
GIEKGGARFITTAFDRAAPKGTGGVKVGGNYAASLLAHKIATEQGYDDCIYLDPATHTKIEEVGAANFFGITHDNAFITP
HSPSILPSITKKSLMVLAKEYLNLKVEEREILMDELGTFKEAGACGTAAIITPIKEIAHNDKSYFFEAPGNTTKQLYDLL
LSIQQGEQEAPKDWIFEVC

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI296010906, Length=346, Percent_Identity=32.6589595375723, Blast_Score=168, Evalue=8e-42,
Organism=Homo sapiens, GI296010904, Length=346, Percent_Identity=32.6589595375723, Blast_Score=168, Evalue=8e-42,
Organism=Homo sapiens, GI38176287, Length=346, Percent_Identity=32.6589595375723, Blast_Score=168, Evalue=8e-42,
Organism=Homo sapiens, GI50658084, Length=345, Percent_Identity=30.1449275362319, Blast_Score=159, Evalue=3e-39,
Organism=Homo sapiens, GI258614015, Length=298, Percent_Identity=31.2080536912752, Blast_Score=142, Evalue=3e-34,
Organism=Homo sapiens, GI296010902, Length=346, Percent_Identity=28.3236994219653, Blast_Score=125, Evalue=5e-29,
Organism=Homo sapiens, GI296010900, Length=346, Percent_Identity=28.3236994219653, Blast_Score=125, Evalue=6e-29,
Organism=Escherichia coli, GI48994963, Length=315, Percent_Identity=32.3809523809524, Blast_Score=128, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI17568601, Length=314, Percent_Identity=31.8471337579618, Blast_Score=149, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17565728, Length=284, Percent_Identity=30.9859154929577, Blast_Score=114, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6322002, Length=318, Percent_Identity=32.3899371069182, Blast_Score=149, Evalue=8e-37,
Organism=Saccharomyces cerevisiae, GI6322608, Length=334, Percent_Identity=32.3353293413174, Blast_Score=147, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24641779, Length=339, Percent_Identity=29.2035398230088, Blast_Score=153, Evalue=2e-37,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005786 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 37449; Mature: 37318

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANLENLDWKNLGFSYIKTDFRFIASYKNGSWSQGELVSENALQISEGSPVLHYGQACFE
CCCCCCCCCCCCCHHHHHHHHHHEEECCCCCCCCCCEECCCCEEECCCCCHHHHHHHHHH
GLKAYRSQKGKALLFRPLENAKRLQTSCERLLMPKVSEELFLRACAEVVKANQKWLAPYK
HHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCC
SGASLYLRPFVIGVGDNLGVKPASEYLFIVFCAPVGAYFKGGIEKGGARFITTAFDRAAP
CCCCEEEEEEEEECCCCCCCCCCCCEEEEEEECCHHHHHHCCCCCCCCEEEEHHHHHCCC
KGTGGVKVGGNYAASLLAHKIATEQGYDDCIYLDPATHTKIEEVGAANFFGITHDNAFIT
CCCCCEEECCHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHCCCCCEEEEECCCEEEC
PHSPSILPSITKKSLMVLAKEYLNLKVEEREILMDELGTFKEAGACGTAAIITPIKEIAH
CCCCCCCHHHHHHHHHHHHHHHHCCEEHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHHHC
NDKSYFFEAPGNTTKQLYDLLLSIQQGEQEAPKDWIFEVC
CCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCHHHHHCCC
>Mature Secondary Structure 
ANLENLDWKNLGFSYIKTDFRFIASYKNGSWSQGELVSENALQISEGSPVLHYGQACFE
CCCCCCCCCCCCHHHHHHHHHHEEECCCCCCCCCCEECCCCEEECCCCCHHHHHHHHHH
GLKAYRSQKGKALLFRPLENAKRLQTSCERLLMPKVSEELFLRACAEVVKANQKWLAPYK
HHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCC
SGASLYLRPFVIGVGDNLGVKPASEYLFIVFCAPVGAYFKGGIEKGGARFITTAFDRAAP
CCCCEEEEEEEEECCCCCCCCCCCCEEEEEEECCHHHHHHCCCCCCCCEEEEHHHHHCCC
KGTGGVKVGGNYAASLLAHKIATEQGYDDCIYLDPATHTKIEEVGAANFFGITHDNAFIT
CCCCCEEECCHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHCCCCCEEEEECCCEEEC
PHSPSILPSITKKSLMVLAKEYLNLKVEEREILMDELGTFKEAGACGTAAIITPIKEIAH
CCCCCCCHHHHHHHHHHHHHHHHCCEEHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHHHC
NDKSYFFEAPGNTTKQLYDLLLSIQQGEQEAPKDWIFEVC
CCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]