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The map label for this gene is rlmN [H]

Identifier: 188528191

GI number: 188528191

Start: 1417939

End: 1419012

Strand: Reverse

Name: rlmN [H]

Synonym: HPSH_07275

Alternate gene names: 188528191

Gene position: 1419012-1417939 (Counterclockwise)

Preceding gene: 188528192

Following gene: 188528190

Centisome position: 88.22

GC content: 38.18

Gene sequence:

>1074_bases
ATGAAAGCGAGTATTTATGATTTCACTCTAAAGGAATTGAGCCAGCTTTTAAAACCAAGCTTTAGGGCTAAACAGCTTTA
TTTGTGGCTCTATGCGAAGTATAAAACAAGCTTTAAAGACATGCAAAATAATTTTTCAAAAGATTTTATCGCTTATTTGG
AGCAAGAATTTACTTTGCGCACGATAGAAATCACGCATGTGAGAGAGAGCGTTGATGGCTCTAAAAAATACCTTTTTAAA
TCTTTAAGAGACAACCACACTTTTGAAGCGGTGTTGTTGAAAATGAAGGATAAAAAGATTGATGAAGAGACGAACGCTAT
TTTAGAGGGGGAAAAATACACCGTATGCGTGTCTTGTCAAATCGGCTGTCAAGTGGGTTGTGCGTTTTGTTTCACTCAAA
AAGGCGGTTTTGTAAGGAACTTAAAAGCGAGCGAGATTATCCAGCAAGCCCTACTCATTAAAGAAGACAATAACCTCCCC
ATTGAAAAAGCGCTCAACATTGTTTTTATGGGAATGGGCGAGCCTTTGAACAATTTAGATGAGGTGTGTAAAGCGATTGA
GATTTTTAATACCGGCATGCAAATTTCACCTAAAAGAATCACGATTTCCACTAGTGGCGTAGCCGATGAAATCCCTATTT
TAGCGGGCAAAAACTTAGGCGTGCAATTAGCCATATCCTTACACGCCGTAGATGACAAAACGCGCTCATCTTTAATGCCC
TTGAATAAAAAATACAATATTGAATGCGTTTTGAATGAAGTGAGGAAATGGCCTTTAGAGCAGCGCAAAAGAGTGATGTT
TGAATACCTTTTGATCAAAGATTTAAACGATAGCCTGGATTGCGCTAAAAAACTTTTAAAACTTTTAAACGGCATTAAAT
CCAAAGTGAATTTGATTTTATTCAACCCGCATGAAGGCTCTAAGTTTGAGCGCCCCAGTTTAGAGAGCGCTAGAATGTTT
GCGGATTTTTTAAACTCCAAAGGCTTATTATGCACCATTAGAGAGTCTAAAGCCTTGGATATTGAAGCGGCTTGCGGGCA
ATTGAGGGAGAAAAAACTCTCCCAACAAATTTAA

Upstream 100 bases:

>100_bases
TAGAGCGCCATAAGATCCAGCTTTTAGTGTGCGTGGATGATCGCAATAAGGTTTTAGGGGTCTTGCACTTGCACCAACTT
TTAGAATTAGGGCTTAAAGC

Downstream 100 bases:

>100_bases
AAACTTTTTTTGTGGCGTTTGTCTTTTTTCTAATGGGGGGCGTTGGTTTCACTCAAGCGATGATTCAAAAAATTAGCGTT
AAAATTCCTTCCATTAACAA

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MKASIYDFTLKELSQLLKPSFRAKQLYLWLYAKYKTSFKDMQNNFSKDFIAYLEQEFTLRTIEITHVRESVDGSKKYLFK
SLRDNHTFEAVLLKMKDKKIDEETNAILEGEKYTVCVSCQIGCQVGCAFCFTQKGGFVRNLKASEIIQQALLIKEDNNLP
IEKALNIVFMGMGEPLNNLDEVCKAIEIFNTGMQISPKRITISTSGVADEIPILAGKNLGVQLAISLHAVDDKTRSSLMP
LNKKYNIECVLNEVRKWPLEQRKRVMFEYLLIKDLNDSLDCAKKLLKLLNGIKSKVNLILFNPHEGSKFERPSLESARMF
ADFLNSKGLLCTIRESKALDIEAACGQLREKKLSQQI

Sequences:

>Translated_357_residues
MKASIYDFTLKELSQLLKPSFRAKQLYLWLYAKYKTSFKDMQNNFSKDFIAYLEQEFTLRTIEITHVRESVDGSKKYLFK
SLRDNHTFEAVLLKMKDKKIDEETNAILEGEKYTVCVSCQIGCQVGCAFCFTQKGGFVRNLKASEIIQQALLIKEDNNLP
IEKALNIVFMGMGEPLNNLDEVCKAIEIFNTGMQISPKRITISTSGVADEIPILAGKNLGVQLAISLHAVDDKTRSSLMP
LNKKYNIECVLNEVRKWPLEQRKRVMFEYLLIKDLNDSLDCAKKLLKLLNGIKSKVNLILFNPHEGSKFERPSLESARMF
ADFLNSKGLLCTIRESKALDIEAACGQLREKKLSQQI
>Mature_357_residues
MKASIYDFTLKELSQLLKPSFRAKQLYLWLYAKYKTSFKDMQNNFSKDFIAYLEQEFTLRTIEITHVRESVDGSKKYLFK
SLRDNHTFEAVLLKMKDKKIDEETNAILEGEKYTVCVSCQIGCQVGCAFCFTQKGGFVRNLKASEIIQQALLIKEDNNLP
IEKALNIVFMGMGEPLNNLDEVCKAIEIFNTGMQISPKRITISTSGVADEIPILAGKNLGVQLAISLHAVDDKTRSSLMP
LNKKYNIECVLNEVRKWPLEQRKRVMFEYLLIKDLNDSLDCAKKLLKLLNGIKSKVNLILFNPHEGSKFERPSLESARMF
ADFLNSKGLLCTIRESKALDIEAACGQLREKKLSQQI

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=364, Percent_Identity=35.4395604395604, Blast_Score=199, Evalue=2e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007197
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 40733; Mature: 40733

Theoretical pI: Translated: 8.92; Mature: 8.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKASIYDFTLKELSQLLKPSFRAKQLYLWLYAKYKTSFKDMQNNFSKDFIAYLEQEFTLR
CCCCCHHHHHHHHHHHHCCCCCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCEEE
TIEITHVRESVDGSKKYLFKSLRDNHTFEAVLLKMKDKKIDEETNAILEGEKYTVCVSCQ
EEEEEEHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHEECCCEEEEEEEEE
IGCQVGCAFCFTQKGGFVRNLKASEIIQQALLIKEDNNLPIEKALNIVFMGMGEPLNNLD
CCCCCCEEEEEECCCCCEECCCHHHHHHHHHHEECCCCCCHHHCCEEEEEECCCCCHHHH
EVCKAIEIFNTGMQISPKRITISTSGVADEIPILAGKNLGVQLAISLHAVDDKTRSSLMP
HHHHHHHHHCCCCEECCCEEEEEECCCCCCCCEEECCCCCEEEEEEEEECCCHHHHHCCC
LNKKYNIECVLNEVRKWPLEQRKRVMFEYLLIKDLNDSLDCAKKLLKLLNGIKSKVNLIL
CCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEEE
FNPHEGSKFERPSLESARMFADFLNSKGLLCTIRESKALDIEAACGQLREKKLSQQI
ECCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKASIYDFTLKELSQLLKPSFRAKQLYLWLYAKYKTSFKDMQNNFSKDFIAYLEQEFTLR
CCCCCHHHHHHHHHHHHCCCCCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCEEE
TIEITHVRESVDGSKKYLFKSLRDNHTFEAVLLKMKDKKIDEETNAILEGEKYTVCVSCQ
EEEEEEHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHEECCCEEEEEEEEE
IGCQVGCAFCFTQKGGFVRNLKASEIIQQALLIKEDNNLPIEKALNIVFMGMGEPLNNLD
CCCCCCEEEEEECCCCCEECCCHHHHHHHHHHEECCCCCCHHHCCEEEEEECCCCCHHHH
EVCKAIEIFNTGMQISPKRITISTSGVADEIPILAGKNLGVQLAISLHAVDDKTRSSLMP
HHHHHHHHHCCCCEECCCEEEEEECCCCCCCCEEECCCCCEEEEEEEEECCCHHHHHCCC
LNKKYNIECVLNEVRKWPLEQRKRVMFEYLLIKDLNDSLDCAKKLLKLLNGIKSKVNLIL
CCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEEE
FNPHEGSKFERPSLESARMFADFLNSKGLLCTIRESKALDIEAACGQLREKKLSQQI
ECCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA