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The map label for this gene is bioB [H]

Identifier: 188528173

GI number: 188528173

Start: 1396155

End: 1397003

Strand: Direct

Name: bioB [H]

Synonym: HPSH_07170

Alternate gene names: 188528173

Gene position: 1396155-1397003 (Clockwise)

Preceding gene: 188528170

Following gene: 188528174

Centisome position: 86.8

GC content: 43.46

Gene sequence:

>849_bases
ATGCAAGAAATTTTTTTATGTTCTATTTCCAATGTGCGCAGTGGGGATTGCAAGGAAGATTGCGCTTATTGCACGCAAAG
CTCACACCACCAAGGAGCGATTAAGCGCTATAAATTCAAAGATGAAAAAGTGGTTTTACAAGAGGCTAGAGCGTTAAGGG
AATTAGGGGCTTTAGGGTTTTGTCTGGTTACTTCAGGGCGCGAATTAGACGATGAAAAATGCGAATATATCGCCAAATTG
GCTAAAGCCATCAATCAAGAAGAATTGGGCTTGCATTTAATCGCATGCTGCGGGCGCGCGGATTTGGAGCAATTAGAGTT
TTTAAGAGATGCGGGCATTCATAGCTATAACCATAATTTAGAGACTTCGCAAAATTTCTTCCCTAAAATTTGTTCCACGC
ACACATGGGAAGAAAGGTTTATCACATGCGAAAACGCTTTAAGGGCGGGGTTAGGCTTGTGCAGTGGGGGGATTTTTGGG
CTTAATGAGAGCTGGGAAGACAGGATTGAAATGCTTAGGGCGTTAGCTTCGCTCTCCCCGCACACCACGCCGATTAATTT
TTTCATTAAAAACCCGGTATTGCCCATTGATACAGAAACTTTAAGCGCGGATGAAGCCCTAGAATGCGTGCTTTTAGCTA
AGGAATTTTTGCCTAACGCTAGGCTTATGGTGGCTGGGGGGCGTGAAGTGGTGTTTAAAGATAACGATAAAAAGGAAGCC
AAGCTTTTTGAATACGGCATCAATGCGGTGGTGTTAGGGGATTATTTAACCACCAAAGGCAAAGCCCCTAAAAAAGATAT
AGAAAAACTGCTCTCTTATGGCTTGACAATGGCGACAAGCTGTCATTGA

Upstream 100 bases:

>100_bases
TAATATTTAATGGAATAAAGCCCTTAAAATCAAAAGCGCTTCTTTTTCTTTGCTATAATCAGTCTAAAAAAACCAACTTT
TTTAAAACAAAGGAATGATG

Downstream 100 bases:

>100_bases
TGAGAGATTTTTTTAAAAGCGTTAGAGGGTTTTTGCACCTTCTTAGAATGATTTTCCCCAAGCACTTTCAAAACGCCTTT
TTGGGCTTGAGCGAATTGTT

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MQEIFLCSISNVRSGDCKEDCAYCTQSSHHQGAIKRYKFKDEKVVLQEARALRELGALGFCLVTSGRELDDEKCEYIAKL
AKAINQEELGLHLIACCGRADLEQLEFLRDAGIHSYNHNLETSQNFFPKICSTHTWEERFITCENALRAGLGLCSGGIFG
LNESWEDRIEMLRALASLSPHTTPINFFIKNPVLPIDTETLSADEALECVLLAKEFLPNARLMVAGGREVVFKDNDKKEA
KLFEYGINAVVLGDYLTTKGKAPKKDIEKLLSYGLTMATSCH

Sequences:

>Translated_282_residues
MQEIFLCSISNVRSGDCKEDCAYCTQSSHHQGAIKRYKFKDEKVVLQEARALRELGALGFCLVTSGRELDDEKCEYIAKL
AKAINQEELGLHLIACCGRADLEQLEFLRDAGIHSYNHNLETSQNFFPKICSTHTWEERFITCENALRAGLGLCSGGIFG
LNESWEDRIEMLRALASLSPHTTPINFFIKNPVLPIDTETLSADEALECVLLAKEFLPNARLMVAGGREVVFKDNDKKEA
KLFEYGINAVVLGDYLTTKGKAPKKDIEKLLSYGLTMATSCH
>Mature_282_residues
MQEIFLCSISNVRSGDCKEDCAYCTQSSHHQGAIKRYKFKDEKVVLQEARALRELGALGFCLVTSGRELDDEKCEYIAKL
AKAINQEELGLHLIACCGRADLEQLEFLRDAGIHSYNHNLETSQNFFPKICSTHTWEERFITCENALRAGLGLCSGGIFG
LNESWEDRIEMLRALASLSPHTTPINFFIKNPVLPIDTETLSADEALECVLLAKEFLPNARLMVAGGREVVFKDNDKKEA
KLFEYGINAVVLGDYLTTKGKAPKKDIEKLLSYGLTMATSCH

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786992, Length=278, Percent_Identity=29.4964028776978, Blast_Score=146, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6321725, Length=285, Percent_Identity=31.2280701754386, Blast_Score=148, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 31506; Mature: 31506

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
4.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQEIFLCSISNVRSGDCKEDCAYCTQSSHHQGAIKRYKFKDEKVVLQEARALRELGALGF
CCCEEEEECCCCCCCCCHHHHHHHHCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCEE
CLVTSGRELDDEKCEYIAKLAKAINQEELGLHLIACCGRADLEQLEFLRDAGIHSYNHNL
EEEECCCCCCHHHHHHHHHHHHHHCHHHHCCHHEECCCCCCHHHHHHHHHCCCCCCCCCC
ETSQNFFPKICSTHTWEERFITCENALRAGLGLCSGGIFGLNESWEDRIEMLRALASLSP
CCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCC
HTTPINFFIKNPVLPIDTETLSADEALECVLLAKEFLPNARLMVAGGREVVFKDNDKKEA
CCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCEEEEECCCCHHH
KLFEYGINAVVLGDYLTTKGKAPKKDIEKLLSYGLTMATSCH
HHHHHCCCEEEECCHHHCCCCCCHHHHHHHHHCCCHHHCCCC
>Mature Secondary Structure
MQEIFLCSISNVRSGDCKEDCAYCTQSSHHQGAIKRYKFKDEKVVLQEARALRELGALGF
CCCEEEEECCCCCCCCCHHHHHHHHCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCEE
CLVTSGRELDDEKCEYIAKLAKAINQEELGLHLIACCGRADLEQLEFLRDAGIHSYNHNL
EEEECCCCCCHHHHHHHHHHHHHHCHHHHCCHHEECCCCCCHHHHHHHHHCCCCCCCCCC
ETSQNFFPKICSTHTWEERFITCENALRAGLGLCSGGIFGLNESWEDRIEMLRALASLSP
CCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCC
HTTPINFFIKNPVLPIDTETLSADEALECVLLAKEFLPNARLMVAGGREVVFKDNDKKEA
CCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCEEEEECCCCHHH
KLFEYGINAVVLGDYLTTKGKAPKKDIEKLLSYGLTMATSCH
HHHHHCCCEEEECCHHHCCCCCCHHHHHHHHHCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA