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The map label for this gene is tenA [H]

Identifier: 188528076

GI number: 188528076

Start: 1311446

End: 1312102

Strand: Direct

Name: tenA [H]

Synonym: HPSH_06665

Alternate gene names: 188528076

Gene position: 1311446-1312102 (Clockwise)

Preceding gene: 188528074

Following gene: 188528077

Centisome position: 81.53

GC content: 36.53

Gene sequence:

>657_bases
ATGCAAGTTTCACAATATCTGTATCAAAATGCGCAATCTATTTGGGGGGATTGTATTTCCCATCCGTTTGTTCAAGGCAT
AGGGCATGGGACTTTAGAAAGAGATAAATTTCGTTTTTATATCATTCAGGATTATTTGTTTCTTTTAGAATACGCTAAGG
TGTTTGCTTTAGGCGTAATTAAAGCTCATGATGAAATTGTGATGAGAGAGTTTTCTAACGCTATACAAGATATTTTGAAT
AACGAGATGAGTATCCATAACCATTACATTAGAGAACTTCAAATCACTCAAATAGAATTGCAAAACGCGTGCCCCACCCT
AGCGAATAAATCCTATACAAGCTACATGCTCGCTGAAGGGATTAAGGGCTCTATTAAAGAAGTTACGGCGGCTGTTTTGG
CTTGTGGCTGGAGCTATTTAGTGATCGCGCAAAATTTAAGCCAAATCTCAAACGCTTTAGAACATGCCTTTTATGGGCAT
TGGATTAAGGGCTATAGTTCCAAAGAATTTCAAGCGTGTGTGTCATGGAATATCAATTTGCTTGATTCCCTCACTCACGC
TTCTTCAAAACAAGAAATTGAGAAGTTAAAGGACATTTTTATCACTACAAGTAAATATGAATACATGTTTTGGGATATGG
CGTATCAAAAAAACTAA

Upstream 100 bases:

>100_bases
AAGCAAGATCCTACCCACCAACCTTTTTAAAAACAAATTTTTATACCATATCACAAGTTTTCGTGTTAGAATGCGTTAAA
TGGATTTTAGGAGTGTAAAA

Downstream 100 bases:

>100_bases
TTCTCATTGATTCATGTTACAATCAAACTAAAGGGGTGCTTTTTAAAAACGCTCAAGTTTTTAAAAGGCTGAGATCAAAC
CCATAGAACTTGTCAAGGTA

Product: putative transcriptional regulator

Products: NA

Alternate protein names: Thiaminase II; Transcriptional activator tenA [H]

Number of amino acids: Translated: 218; Mature: 218

Protein sequence:

>218_residues
MQVSQYLYQNAQSIWGDCISHPFVQGIGHGTLERDKFRFYIIQDYLFLLEYAKVFALGVIKAHDEIVMREFSNAIQDILN
NEMSIHNHYIRELQITQIELQNACPTLANKSYTSYMLAEGIKGSIKEVTAAVLACGWSYLVIAQNLSQISNALEHAFYGH
WIKGYSSKEFQACVSWNINLLDSLTHASSKQEIEKLKDIFITTSKYEYMFWDMAYQKN

Sequences:

>Translated_218_residues
MQVSQYLYQNAQSIWGDCISHPFVQGIGHGTLERDKFRFYIIQDYLFLLEYAKVFALGVIKAHDEIVMREFSNAIQDILN
NEMSIHNHYIRELQITQIELQNACPTLANKSYTSYMLAEGIKGSIKEVTAAVLACGWSYLVIAQNLSQISNALEHAFYGH
WIKGYSSKEFQACVSWNINLLDSLTHASSKQEIEKLKDIFITTSKYEYMFWDMAYQKN
>Mature_218_residues
MQVSQYLYQNAQSIWGDCISHPFVQGIGHGTLERDKFRFYIIQDYLFLLEYAKVFALGVIKAHDEIVMREFSNAIQDILN
NEMSIHNHYIRELQITQIELQNACPTLANKSYTSYMLAEGIKGSIKEVTAAVLACGWSYLVIAQNLSQISNALEHAFYGH
WIKGYSSKEFQACVSWNINLLDSLTHASSKQEIEKLKDIFITTSKYEYMFWDMAYQKN

Specific function: Stimulates the production of several extracellular degradative enzymes at the transcriptional level, probably by interfering with degS and degU, which are required for this effect. Is not an essential protein, but affects the sporulation frequency [H]

COG id: COG0819

COG function: function code K; Putative transcription activator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiaminase-2 family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6325378, Length=205, Percent_Identity=25.8536585365854, Blast_Score=74, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324517, Length=205, Percent_Identity=25.8536585365854, Blast_Score=73, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6324997, Length=205, Percent_Identity=25.3658536585366, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016084
- InterPro:   IPR004305 [H]

Pfam domain/function: PF03070 TENA_THI-4 [H]

EC number: =3.5.99.2 [H]

Molecular weight: Translated: 25194; Mature: 25194

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVSQYLYQNAQSIWGDCISHPFVQGIGHGTLERDKFRFYIIQDYLFLLEYAKVFALGVI
CCHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KAHDEIVMREFSNAIQDILNNEMSIHNHYIRELQITQIELQNACPTLANKSYTSYMLAEG
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHCCCCHHHHHHHHC
IKGSIKEVTAAVLACGWSYLVIAQNLSQISNALEHAFYGHWIKGYSSKEFQACVSWNINL
CCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHH
LDSLTHASSKQEIEKLKDIFITTSKYEYMFWDMAYQKN
HHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEEHHHCCC
>Mature Secondary Structure
MQVSQYLYQNAQSIWGDCISHPFVQGIGHGTLERDKFRFYIIQDYLFLLEYAKVFALGVI
CCHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KAHDEIVMREFSNAIQDILNNEMSIHNHYIRELQITQIELQNACPTLANKSYTSYMLAEG
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHCCCCHHHHHHHHC
IKGSIKEVTAAVLACGWSYLVIAQNLSQISNALEHAFYGHWIKGYSSKEFQACVSWNINL
CCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHH
LDSLTHASSKQEIEKLKDIFITTSKYEYMFWDMAYQKN
HHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEEHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1898926; 9384377 [H]