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The map label for this gene is trpB [H]

Identifier: 188528067

GI number: 188528067

Start: 1302615

End: 1303796

Strand: Reverse

Name: trpB [H]

Synonym: HPSH_06620

Alternate gene names: 188528067

Gene position: 1303796-1302615 (Counterclockwise)

Preceding gene: 188528068

Following gene: 188528066

Centisome position: 81.05

GC content: 43.4

Gene sequence:

>1182_bases
ATGAATAAAAAAGCGTATTTTGGGGAGTTTGGAGGGAGTTTTGTTTCAGAATTGTTAGTGCCTGCATTAAGAGAGTTAGA
ACAGGCGTTTGATGCGTGTTTGAAAGATGAGGAATTCCAAAAAGAATATTTCCATCTTTTAAAAGATTTTGTGGGCCGTC
CTAGCCCCTTAACCTTGTGTCAAAATATCGTTTCTAACCCTAAAGTTAAGCTTTATCTAAAACGAGAAGATTTAATCCAT
GGCGGGGCGCATAAGACTAATCAAGCCTTAGGGCAAGCCCTTTTAGCGAAAAAAATGGGTAAAACAAGGATTATTGCTGA
AACAGGTGCTGGGCAGCATGGCGTGGCGACGGCTATCGCTTGCGCGTTGTTGGGTTTAAAATGCGTGATTTTTATGGGGG
CTAAAGACATCAAGCGCCAGGAAATGAATGTTTTTAGAATGCGTTTATTAGGTGCTGAAGTAGAAGAGGTTAATTCAGGG
AGCGCGACGCTTAAAGACGCTGTGAATGAAGCCTTAAGGGATTGGGCGAGCAGTTACAAGGACACGCATTATTTGCTAGG
CACAGCCGCCGGGCCACACCCTTACCCCACAATGGTTAAAACCTTTCAAAAAATGATGGGCGATGAGGTTAAAAGCCAAA
TTTTAGAAAAAGAAAACCGCTTGCCTGATTATGTTATCGCATGCGTTGGAGGGGGGTCTAACGCTATAGGGGTATTTAGT
GCGTTTTTAAACGATAAAGAGGTTAAACTCATAGGCGTAGAGCCGGCGGGTTTAGGGCTAGAAACCAATAAGCATGGGGC
GACTTTGAATAAGGGGCGTGTGGGGATTTTGCATGGGAATAAAACCTATCTTTTACAAGATGATGAAGGCCAGATTATAG
AAAGCCATAGCATTAGCGCCGGGCTTGATTATCCAGGAGTGGGGCCAGAACACAGCTATTTAAAAGAGAGCGCTCGTGCG
ATTTATGAAAGCGCAAGCGATTTTGAAGCGCTAGAAGCCTTTAGTTTGTTGTGCCAAAAAGAAGGCATTATCCCAGCACT
AGAAAGCTCACACGCCTTAGCGTATGCCTTAAAACTCGCTCAAAAATGCGAAGAAGAAAGCATCATGGTAGTGAATTTAA
GCGGTCGGGGGGATAAGGATTTAAGCACCGTTTATAACGCTTTAAAAGGAGGTTTAAAATGA

Upstream 100 bases:

>100_bases
GCGTTGGGTTTGGATTTCAATTCGGGTTTGGAAACAAGCCCCGGGATTAAAAATAAGGATAAAATCAAGCAATTAGCCCG
AATTTTAAGAGAGTATTAAA

Downstream 100 bases:

>100_bases
GATATAAAAACATGTTTGAAACATTAAAAAAACAAGAAAAAATGGCGTTTATCCCATTTGTAACCTTGGGCGATCCTAAT
TATGCATTGAGTTTTGAAAT

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MNKKAYFGEFGGSFVSELLVPALRELEQAFDACLKDEEFQKEYFHLLKDFVGRPSPLTLCQNIVSNPKVKLYLKREDLIH
GGAHKTNQALGQALLAKKMGKTRIIAETGAGQHGVATAIACALLGLKCVIFMGAKDIKRQEMNVFRMRLLGAEVEEVNSG
SATLKDAVNEALRDWASSYKDTHYLLGTAAGPHPYPTMVKTFQKMMGDEVKSQILEKENRLPDYVIACVGGGSNAIGVFS
AFLNDKEVKLIGVEPAGLGLETNKHGATLNKGRVGILHGNKTYLLQDDEGQIIESHSISAGLDYPGVGPEHSYLKESARA
IYESASDFEALEAFSLLCQKEGIIPALESSHALAYALKLAQKCEEESIMVVNLSGRGDKDLSTVYNALKGGLK

Sequences:

>Translated_393_residues
MNKKAYFGEFGGSFVSELLVPALRELEQAFDACLKDEEFQKEYFHLLKDFVGRPSPLTLCQNIVSNPKVKLYLKREDLIH
GGAHKTNQALGQALLAKKMGKTRIIAETGAGQHGVATAIACALLGLKCVIFMGAKDIKRQEMNVFRMRLLGAEVEEVNSG
SATLKDAVNEALRDWASSYKDTHYLLGTAAGPHPYPTMVKTFQKMMGDEVKSQILEKENRLPDYVIACVGGGSNAIGVFS
AFLNDKEVKLIGVEPAGLGLETNKHGATLNKGRVGILHGNKTYLLQDDEGQIIESHSISAGLDYPGVGPEHSYLKESARA
IYESASDFEALEAFSLLCQKEGIIPALESSHALAYALKLAQKCEEESIMVVNLSGRGDKDLSTVYNALKGGLK
>Mature_393_residues
MNKKAYFGEFGGSFVSELLVPALRELEQAFDACLKDEEFQKEYFHLLKDFVGRPSPLTLCQNIVSNPKVKLYLKREDLIH
GGAHKTNQALGQALLAKKMGKTRIIAETGAGQHGVATAIACALLGLKCVIFMGAKDIKRQEMNVFRMRLLGAEVEEVNSG
SATLKDAVNEALRDWASSYKDTHYLLGTAAGPHPYPTMVKTFQKMMGDEVKSQILEKENRLPDYVIACVGGGSNAIGVFS
AFLNDKEVKLIGVEPAGLGLETNKHGATLNKGRVGILHGNKTYLLQDDEGQIIESHSISAGLDYPGVGPEHSYLKESARA
IYESASDFEALEAFSLLCQKEGIIPALESSHALAYALKLAQKCEEESIMVVNLSGRGDKDLSTVYNALKGGLK

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine [H]

COG id: COG0133

COG function: function code E; Tryptophan synthase beta chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family [H]

Homologues:

Organism=Escherichia coli, GI1787515, Length=385, Percent_Identity=66.7532467532468, Blast_Score=536, Evalue=1e-154,
Organism=Saccharomyces cerevisiae, GI6321412, Length=384, Percent_Identity=53.90625, Blast_Score=429, Evalue=1e-121,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR006653
- InterPro:   IPR006654
- InterPro:   IPR023026 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 42727; Mature: 42727

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKAYFGEFGGSFVSELLVPALRELEQAFDACLKDEEFQKEYFHLLKDFVGRPSPLTLC
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHH
QNIVSNPKVKLYLKREDLIHGGAHKTNQALGQALLAKKMGKTRIIAETGAGQHGVATAIA
HHHHCCCCEEEEEEHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHH
CALLGLKCVIFMGAKDIKRQEMNVFRMRLLGAEVEEVNSGSATLKDAVNEALRDWASSYK
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHHHHHHCC
DTHYLLGTAAGPHPYPTMVKTFQKMMGDEVKSQILEKENRLPDYVIACVGGGSNAIGVFS
CCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHH
AFLNDKEVKLIGVEPAGLGLETNKHGATLNKGRVGILHGNKTYLLQDDEGQIIESHSISA
HHHCCCCEEEEEECCCCCCCCCCCCCCEECCCEEEEEECCEEEEEECCCCCEEECCCCCC
GLDYPGVGPEHSYLKESARAIYESASDFEALEAFSLLCQKEGIIPALESSHALAYALKLA
CCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
QKCEEESIMVVNLSGRGDKDLSTVYNALKGGLK
HHCCCCCEEEEEECCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNKKAYFGEFGGSFVSELLVPALRELEQAFDACLKDEEFQKEYFHLLKDFVGRPSPLTLC
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHH
QNIVSNPKVKLYLKREDLIHGGAHKTNQALGQALLAKKMGKTRIIAETGAGQHGVATAIA
HHHHCCCCEEEEEEHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHH
CALLGLKCVIFMGAKDIKRQEMNVFRMRLLGAEVEEVNSGSATLKDAVNEALRDWASSYK
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHHHHHHCC
DTHYLLGTAAGPHPYPTMVKTFQKMMGDEVKSQILEKENRLPDYVIACVGGGSNAIGVFS
CCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHH
AFLNDKEVKLIGVEPAGLGLETNKHGATLNKGRVGILHGNKTYLLQDDEGQIIESHSISA
HHHCCCCEEEEEECCCCCCCCCCCCCCEECCCEEEEEECCEEEEEECCCCCEEECCCCCC
GLDYPGVGPEHSYLKESARAIYESASDFEALEAFSLLCQKEGIIPALESSHALAYALKLA
CCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
QKCEEESIMVVNLSGRGDKDLSTVYNALKGGLK
HHCCCCCEEEEEECCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]