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The map label for this gene is trpA

Identifier: 188528066

GI number: 188528066

Start: 1301830

End: 1302618

Strand: Reverse

Name: trpA

Synonym: HPSH_06615

Alternate gene names: 188528066

Gene position: 1302618-1301830 (Counterclockwise)

Preceding gene: 188528067

Following gene: 188528043

Centisome position: 80.98

GC content: 40.56

Gene sequence:

>789_bases
ATGAGATATAAAAACATGTTTGAAACATTAAAAAAACAAGAAAAAATGGCGTTTATCCCATTTGTAACCTTGGGCGATCC
TAATTATGCATTGAGTTTTGAAATCGTTAAAACTTTAATTGCTAGCGGGGTGAGCGCTTTAGAATTGGGGTTTGCTTTCT
CTGATCCGGTAGCGGATGGCGTTACCATACAAGCGAGCCATTTAAGGGCGTTAAAACACGCTAGCATGGCTAAAAATTTC
CAGCTTTTAAGGGCGATCAGAGATTACAATCCCCACATTCCCATAGGGCTTTTAGCGTATGCGAATCTCATTTTTTCTTA
TGGCGTGGATAATTTTTACGCCCAAATCAAAGAATGCGGCGTGGATAGCGTTTTAATAGCGGACATGCCCCTAATAGAAA
AAGAATTAGTCCTAAAATCCGCTCAAAAACACCAGATTAAACAAATCTTTATCGCCAGCCCCAATGCGAGTAATAAAGAT
TTAGAACGAGCCGCTATGCATTCGCAGGGCTATATCTACACTCTGGCTAGGAGTGGGGTTACAGGAGCGAGCCGTATTTT
AGAAAATGACGCGAGCGCTATTATTAAAACCTTAAAAACTTTTAGCCCCACACCGGCCTTACTGGGCTTTGGCATTTCTA
AAAAAGAACACATAAAAAACGCTAAAGGCATGGGCGCTGATGGCGTGATTTGCGGCTCAGCGTTAGTCAAAATCATAGAA
GAAAATTTGAACGATGAAAACGCCATGCTAGAAAGAATTAAAGGGTTTATAGGAGAAATGATCTCTTAA

Upstream 100 bases:

>100_bases
CTCAAAAATGCGAAGAAGAAAGCATCATGGTAGTGAATTTAAGCGGTCGGGGGGATAAGGATTTAAGCACCGTTTATAAC
GCTTTAAAAGGAGGTTTAAA

Downstream 100 bases:

>100_bases
AGGCTTTTATTGTTTCAAAAGACTAAAAATCATCAGGCTTTATTGCATGCACCTTATTATGGCGTTAGCGTAGAAAATGC
CGCCATAAACCTAACAACAA

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MRYKNMFETLKKQEKMAFIPFVTLGDPNYALSFEIVKTLIASGVSALELGFAFSDPVADGVTIQASHLRALKHASMAKNF
QLLRAIRDYNPHIPIGLLAYANLIFSYGVDNFYAQIKECGVDSVLIADMPLIEKELVLKSAQKHQIKQIFIASPNASNKD
LERAAMHSQGYIYTLARSGVTGASRILENDASAIIKTLKTFSPTPALLGFGISKKEHIKNAKGMGADGVICGSALVKIIE
ENLNDENAMLERIKGFIGEMIS

Sequences:

>Translated_262_residues
MRYKNMFETLKKQEKMAFIPFVTLGDPNYALSFEIVKTLIASGVSALELGFAFSDPVADGVTIQASHLRALKHASMAKNF
QLLRAIRDYNPHIPIGLLAYANLIFSYGVDNFYAQIKECGVDSVLIADMPLIEKELVLKSAQKHQIKQIFIASPNASNKD
LERAAMHSQGYIYTLARSGVTGASRILENDASAIIKTLKTFSPTPALLGFGISKKEHIKNAKGMGADGVICGSALVKIIE
ENLNDENAMLERIKGFIGEMIS
>Mature_262_residues
MRYKNMFETLKKQEKMAFIPFVTLGDPNYALSFEIVKTLIASGVSALELGFAFSDPVADGVTIQASHLRALKHASMAKNF
QLLRAIRDYNPHIPIGLLAYANLIFSYGVDNFYAQIKECGVDSVLIADMPLIEKELVLKSAQKHQIKQIFIASPNASNKD
LERAAMHSQGYIYTLARSGVTGASRILENDASAIIKTLKTFSPTPALLGFGISKKEHIKNAKGMGADGVICGSALVKIIE
ENLNDENAMLERIKGFIGEMIS

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate [H]

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family [H]

Homologues:

Organism=Escherichia coli, GI1787514, Length=260, Percent_Identity=46.5384615384615, Blast_Score=226, Evalue=8e-61,
Organism=Saccharomyces cerevisiae, GI6321412, Length=237, Percent_Identity=29.957805907173, Blast_Score=125, Evalue=7e-30,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028 [H]

Pfam domain/function: PF00290 Trp_syntA [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 28700; Mature: 28700

Theoretical pI: Translated: 8.96; Mature: 8.96

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYKNMFETLKKQEKMAFIPFVTLGDPNYALSFEIVKTLIASGVSALELGFAFSDPVADG
CCHHHHHHHHHHHCCEEEEEEEEECCCCCEEHHHHHHHHHHCCHHHHHHCCCCCCCCCCC
VTIQASHLRALKHASMAKNFQLLRAIRDYNPHIPIGLLAYANLIFSYGVDNFYAQIKECG
EEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHCC
VDSVLIADMPLIEKELVLKSAQKHQIKQIFIASPNASNKDLERAAMHSQGYIYTLARSGV
CCEEEECCCCHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHCCCEEEEEECCCC
TGASRILENDASAIIKTLKTFSPTPALLGFGISKKEHIKNAKGMGADGVICGSALVKIIE
CHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHH
ENLNDENAMLERIKGFIGEMIS
HCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRYKNMFETLKKQEKMAFIPFVTLGDPNYALSFEIVKTLIASGVSALELGFAFSDPVADG
CCHHHHHHHHHHHCCEEEEEEEEECCCCCEEHHHHHHHHHHCCHHHHHHCCCCCCCCCCC
VTIQASHLRALKHASMAKNFQLLRAIRDYNPHIPIGLLAYANLIFSYGVDNFYAQIKECG
EEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHCC
VDSVLIADMPLIEKELVLKSAQKHQIKQIFIASPNASNKDLERAAMHSQGYIYTLARSGV
CCEEEECCCCHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHCCCEEEEEECCCC
TGASRILENDASAIIKTLKTFSPTPALLGFGISKKEHIKNAKGMGADGVICGSALVKIIE
CHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHH
ENLNDENAMLERIKGFIGEMIS
HCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA