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The map label for this gene is alaS

Identifier: 188528029

GI number: 188528029

Start: 1263899

End: 1266442

Strand: Reverse

Name: alaS

Synonym: HPSH_06420

Alternate gene names: 188528029

Gene position: 1266442-1263899 (Counterclockwise)

Preceding gene: 188528031

Following gene: 188528028

Centisome position: 78.73

GC content: 42.73

Gene sequence:

>2544_bases
ATGGATATTCGCAACGAATTTTTACAATTTTTCCACAATAAGGGGCATGCCGTTTATCCTAGCATGCCTTTAGTGCCTAA
TGACGCTACCTTGCTTTTCACTAATGCCGGCATGGTGCAATTTAAAGATATTTTTACCGGGATTGTGCCACGCCCTAGCA
TTCCTAGAGCGACAAGTTCGCAATTGTGCATGCGAGCGGGTGGCAAGCATAACGATTTGGAAAATGTCGGTTACACCGCA
AGACACCACACGCTTTTTGAAATGCTAGGGAATTTCTCTTTTGGGGATTATTTCAAAGAAGAAGCGATCTTGTTTGCGTG
GGAATTTGTAACCAAAAATTTAGGGTTTAAGTCTAAAGATTTATATATCAGCGTGCATGAAAAAGACGATGAAGCCGTCA
AGCTGTGGGAAAAGTTTGTGCCTTTTGAGAGGATTAAAAAAATGGGCGATAAAGATAATTTTTGGCAAATGGGCGATAGC
GGGCCTTGCGGGCCTTGCAGTGAAATTTACATTGATCAGGGCGAAAAACACTTTAAGGGGAGCGAGGATTATTTTGGGGG
CGAGGGCGATAGGTTTTTAGAAATTTGGAATTTGGTGTTCATGCAATACGAACGCTCTAATGATGGCGTTTTGTCCCCCT
TGCCAAAGCCTAGCATTGATACAGGCATGGGATTAGAAAGGGTGCAAGCGCTATTAGAACACAAGCTCAATAATTTTGAT
TCTTCATTATTTGCGCCCTTAATGGAAGAAATCAGCGATCTTGCAAGCCTGGATTATGCGAGCGAGTTCCAACCAAGCTT
TAGGGTGGTAGCCGATCACGCAAGAGCGGTAGCGTTTTTGCTCGCTCAAGGGGTGCATTTCAATAAAGAAGGCCGTGGCT
ATGTTTTAAGGCGCATTTTAAGGCGAGCCTTAAGGCATGGGTATTTAATGGGCTTGAAAGAAGCGTTTTTATACAAAGTC
GTGGGCGTGGTGTGCGAGCAATTTTCTAACACGCATGCGTATTTGAAAGAATCTAAAGAAATGGTAATGAAAGAATGCTT
TGAAGAAGAAGAGCGCTTTTTAGAGACTTTGGAATCGGGCATGGAATTGTTTAACTTGTCTTTAGAGCATTTGAATGAAA
ATAAAATTTTTGATGGCAAGATCGCTTTCAAGCTTTATGACACTTTTGGTTTTCCTTTGGATTTAACGAACGACATGCTA
AGAAGTCATGGGGCGTGCGTGGATATGCAAGGTTTTGAATCTTGCATGCAAGAGCAAGTGAAACGCTCTAAAGCTTCATG
GAAAGGCAAACAAAATAACGCTGATTTTAGCGCTATTTTAAACGCTTATGCGCCCAATGTTTTTGTGGGGTATGAAACGA
CAGAATGTTGTGCTAAAGTTTTAGGGTTTTTTGATAGCGATTTTAAAGAAATAACCGATGCAAAGCCTAACCAAGAAGTC
TGGGTGTTGCTAGAAAAAACCCCTTTTTACGCTGAGGGTGGGGGGGCTATAGGCGATAGGGGCGCGCTTTTAAAGGACGA
TGAAGAAGCGGCTATAGTGTTGGATACAAAAAACTTTTTTGGGCTTAATTTTTCGCTCCTTGAAATCAAAAAAGCGCTAA
AAAAAGGCGATCAAGTGATCGCACAAGTGAGCGATGAGCGCTTAGAAATTGCCAAACACCATAGCGCGACGCATTTATTG
CAGAGCGCTTTAAGAGAAGTTTTAGGATCGCATGTGAGTCAAGCAGGGAGTTTAGTGGAATCCAAACGATTGCGCTTTGA
TTTTTCGCACCCTAAAGCGCTCAATGATGAAGAGTTAGAAAAAGTAGAAGATTTAGTTAATGCTCAAATCTTCAAGCACC
TAAATAGCCAGGTGGAGCATATGCCCTTAAATCAGGCTAAAGATAAGGGAGCGTTAGCGTTATTTAGTGAAAAATACGCT
GAAAATGTGCGGGTGGTGAGTTTTAAAGAAGCGTCCATTGAATTGTGTGGGGGCATTCATGTGGAAAATACCGGGCTGAT
TGGGGGGTTTAGGATTGTAAAAGAGAGCGGGGTGAGTAGTGGGGTCAGACGCATTGAAGCGGTGTGCGGGAAAGCCTTTT
ACCAACTGGCTAAAGAAGAGAATAAAGAGCTTAAAAACGCTAAGATTGCATTAAAAAATAACGATGTGATAGCCGGCATT
AACAAGCTTAAAGAGAGCGTGAAAAACAGCCAAAAAGCCCCCGTTTCTGTGGAGTTACCGGTTGAAAAAATCCATGGCGT
GAATTTAGTGGTGGGCGTAGTGGAACAGGGCGACATTAAAGAAATGATTGACCGGTTGAAAAGTAAGCATGAAAGATTGC
TCGCTATGGTGTTTAAAAAAGAAAATGAGCGAATAACTCTCGCATGCGGGGTGAAAAACGCGCCTATAAAAGCGAATGTG
TGGGCTAATGAAGTGGCGCAAATTTTAGGGGGCAAAGGAGGCGGAAGAGGCGATTTTGCGAGCGCTGGAGGCAGGGATAT
TGAAAATTTGCAAGCCGCGCTCAATTTGGCGAAAAATACCGCTCTTAAAGCTTTAGAGGGATAA

Upstream 100 bases:

>100_bases
TGGATGTTTAAAGTATAATAAACTATCTTTTTAAAAAAACAACTTAAAAGAGTTATAAATAGCCTTAAAATGCACGATAA
AACAACAAAAAGGAATACCC

Downstream 100 bases:

>100_bases
CATGGAGCTTATTTTAGGCTCTCAATCTAGCGCTAGGGCGAATCTTTTAAAAGAGCATGGGATTGAGTTTGAACAAAAAG
CGCTCTATTTTGATGAAGAA

Product: alanyl-tRNA synthetase

Products: NA

Alternate protein names: Alanine--tRNA ligase; AlaRS [H]

Number of amino acids: Translated: 847; Mature: 847

Protein sequence:

>847_residues
MDIRNEFLQFFHNKGHAVYPSMPLVPNDATLLFTNAGMVQFKDIFTGIVPRPSIPRATSSQLCMRAGGKHNDLENVGYTA
RHHTLFEMLGNFSFGDYFKEEAILFAWEFVTKNLGFKSKDLYISVHEKDDEAVKLWEKFVPFERIKKMGDKDNFWQMGDS
GPCGPCSEIYIDQGEKHFKGSEDYFGGEGDRFLEIWNLVFMQYERSNDGVLSPLPKPSIDTGMGLERVQALLEHKLNNFD
SSLFAPLMEEISDLASLDYASEFQPSFRVVADHARAVAFLLAQGVHFNKEGRGYVLRRILRRALRHGYLMGLKEAFLYKV
VGVVCEQFSNTHAYLKESKEMVMKECFEEEERFLETLESGMELFNLSLEHLNENKIFDGKIAFKLYDTFGFPLDLTNDML
RSHGACVDMQGFESCMQEQVKRSKASWKGKQNNADFSAILNAYAPNVFVGYETTECCAKVLGFFDSDFKEITDAKPNQEV
WVLLEKTPFYAEGGGAIGDRGALLKDDEEAAIVLDTKNFFGLNFSLLEIKKALKKGDQVIAQVSDERLEIAKHHSATHLL
QSALREVLGSHVSQAGSLVESKRLRFDFSHPKALNDEELEKVEDLVNAQIFKHLNSQVEHMPLNQAKDKGALALFSEKYA
ENVRVVSFKEASIELCGGIHVENTGLIGGFRIVKESGVSSGVRRIEAVCGKAFYQLAKEENKELKNAKIALKNNDVIAGI
NKLKESVKNSQKAPVSVELPVEKIHGVNLVVGVVEQGDIKEMIDRLKSKHERLLAMVFKKENERITLACGVKNAPIKANV
WANEVAQILGGKGGGRGDFASAGGRDIENLQAALNLAKNTALKALEG

Sequences:

>Translated_847_residues
MDIRNEFLQFFHNKGHAVYPSMPLVPNDATLLFTNAGMVQFKDIFTGIVPRPSIPRATSSQLCMRAGGKHNDLENVGYTA
RHHTLFEMLGNFSFGDYFKEEAILFAWEFVTKNLGFKSKDLYISVHEKDDEAVKLWEKFVPFERIKKMGDKDNFWQMGDS
GPCGPCSEIYIDQGEKHFKGSEDYFGGEGDRFLEIWNLVFMQYERSNDGVLSPLPKPSIDTGMGLERVQALLEHKLNNFD
SSLFAPLMEEISDLASLDYASEFQPSFRVVADHARAVAFLLAQGVHFNKEGRGYVLRRILRRALRHGYLMGLKEAFLYKV
VGVVCEQFSNTHAYLKESKEMVMKECFEEEERFLETLESGMELFNLSLEHLNENKIFDGKIAFKLYDTFGFPLDLTNDML
RSHGACVDMQGFESCMQEQVKRSKASWKGKQNNADFSAILNAYAPNVFVGYETTECCAKVLGFFDSDFKEITDAKPNQEV
WVLLEKTPFYAEGGGAIGDRGALLKDDEEAAIVLDTKNFFGLNFSLLEIKKALKKGDQVIAQVSDERLEIAKHHSATHLL
QSALREVLGSHVSQAGSLVESKRLRFDFSHPKALNDEELEKVEDLVNAQIFKHLNSQVEHMPLNQAKDKGALALFSEKYA
ENVRVVSFKEASIELCGGIHVENTGLIGGFRIVKESGVSSGVRRIEAVCGKAFYQLAKEENKELKNAKIALKNNDVIAGI
NKLKESVKNSQKAPVSVELPVEKIHGVNLVVGVVEQGDIKEMIDRLKSKHERLLAMVFKKENERITLACGVKNAPIKANV
WANEVAQILGGKGGGRGDFASAGGRDIENLQAALNLAKNTALKALEG
>Mature_847_residues
MDIRNEFLQFFHNKGHAVYPSMPLVPNDATLLFTNAGMVQFKDIFTGIVPRPSIPRATSSQLCMRAGGKHNDLENVGYTA
RHHTLFEMLGNFSFGDYFKEEAILFAWEFVTKNLGFKSKDLYISVHEKDDEAVKLWEKFVPFERIKKMGDKDNFWQMGDS
GPCGPCSEIYIDQGEKHFKGSEDYFGGEGDRFLEIWNLVFMQYERSNDGVLSPLPKPSIDTGMGLERVQALLEHKLNNFD
SSLFAPLMEEISDLASLDYASEFQPSFRVVADHARAVAFLLAQGVHFNKEGRGYVLRRILRRALRHGYLMGLKEAFLYKV
VGVVCEQFSNTHAYLKESKEMVMKECFEEEERFLETLESGMELFNLSLEHLNENKIFDGKIAFKLYDTFGFPLDLTNDML
RSHGACVDMQGFESCMQEQVKRSKASWKGKQNNADFSAILNAYAPNVFVGYETTECCAKVLGFFDSDFKEITDAKPNQEV
WVLLEKTPFYAEGGGAIGDRGALLKDDEEAAIVLDTKNFFGLNFSLLEIKKALKKGDQVIAQVSDERLEIAKHHSATHLL
QSALREVLGSHVSQAGSLVESKRLRFDFSHPKALNDEELEKVEDLVNAQIFKHLNSQVEHMPLNQAKDKGALALFSEKYA
ENVRVVSFKEASIELCGGIHVENTGLIGGFRIVKESGVSSGVRRIEAVCGKAFYQLAKEENKELKNAKIALKNNDVIAGI
NKLKESVKNSQKAPVSVELPVEKIHGVNLVVGVVEQGDIKEMIDRLKSKHERLLAMVFKKENERITLACGVKNAPIKANV
WANEVAQILGGKGGGRGDFASAGGRDIENLQAALNLAKNTALKALEG

Specific function: Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction:alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editin

COG id: COG0013

COG function: function code J; Alanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI109148542, Length=963, Percent_Identity=35.4101765316719, Blast_Score=479, Evalue=1e-135,
Organism=Homo sapiens, GI38569417, Length=954, Percent_Identity=32.1802935010482, Blast_Score=407, Evalue=1e-113,
Organism=Escherichia coli, GI1789048, Length=863, Percent_Identity=46.3499420625724, Blast_Score=697, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17506981, Length=968, Percent_Identity=33.6776859504132, Blast_Score=462, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI17536681, Length=737, Percent_Identity=32.5644504748982, Blast_Score=332, Evalue=5e-91,
Organism=Saccharomyces cerevisiae, GI6324911, Length=751, Percent_Identity=40.3462050599201, Blast_Score=497, Evalue=1e-141,
Organism=Drosophila melanogaster, GI24582809, Length=972, Percent_Identity=33.0246913580247, Blast_Score=460, Evalue=1e-129,
Organism=Drosophila melanogaster, GI45552267, Length=972, Percent_Identity=33.0246913580247, Blast_Score=460, Evalue=1e-129,
Organism=Drosophila melanogaster, GI24658214, Length=794, Percent_Identity=29.4710327455919, Blast_Score=283, Evalue=4e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002318
- InterPro:   IPR018162
- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR023033
- InterPro:   IPR003156
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF02272 DHHA1; PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: =6.1.1.7 [H]

Molecular weight: Translated: 94831; Mature: 94831

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIRNEFLQFFHNKGHAVYPSMPLVPNDATLLFTNAGMVQFKDIFTGIVPRPSIPRATSS
CCHHHHHHHHHHCCCCEECCCCCCCCCCCEEEEECCCCHHHHHHHHCCCCCCCCCCCCHH
QLCMRAGGKHNDLENVGYTARHHTLFEMLGNFSFGDYFKEEAILFAWEFVTKNLGFKSKD
HHHHHCCCCCCCHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
LYISVHEKDDEAVKLWEKFVPFERIKKMGDKDNFWQMGDSGPCGPCSEIYIDQGEKHFKG
EEEEEECCCHHHHHHHHHHCCHHHHHHCCCCCCCEECCCCCCCCCCCHHHCCCCCHHCCC
SEDYFGGEGDRFLEIWNLVFMQYERSNDGVLSPLPKPSIDTGMGLERVQALLEHKLNNFD
CCCCCCCCCHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
SSLFAPLMEEISDLASLDYASEFQPSFRVVADHARAVAFLLAQGVHFNKEGRGYVLRRIL
HHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
RRALRHGYLMGLKEAFLYKVVGVVCEQFSNTHAYLKESKEMVMKECFEEEERFLETLESG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MELFNLSLEHLNENKIFDGKIAFKLYDTFGFPLDLTNDMLRSHGACVDMQGFESCMQEQV
HHHHHHHHHHCCCCCEECCEEEEEEEECCCCCCCCHHHHHHHCCCEEEHHHHHHHHHHHH
KRSKASWKGKQNNADFSAILNAYAPNVFVGYETTECCAKVLGFFDSDFKEITDAKPNQEV
HHHHHHCCCCCCCCHHHHHHHHHCCCEEEECCHHHHHHHHHHHHCCHHHHHHCCCCCCEE
WVLLEKTPFYAEGGGAIGDRGALLKDDEEAAIVLDTKNFFGLNFSLLEIKKALKKGDQVI
EEEEECCCCEECCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHH
AQVSDERLEIAKHHSATHLLQSALREVLGSHVSQAGSLVESKRLRFDFSHPKALNDEELE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHH
KVEDLVNAQIFKHLNSQVEHMPLNQAKDKGALALFSEKYAENVRVVSFKEASIELCGGIH
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCHHHHCCEE
VENTGLIGGFRIVKESGVSSGVRRIEAVCGKAFYQLAKEENKELKNAKIALKNNDVIAGI
ECCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEHHH
NKLKESVKNSQKAPVSVELPVEKIHGVNLVVGVVEQGDIKEMIDRLKSKHERLLAMVFKK
HHHHHHHHCCCCCCEEEECCHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHC
ENERITLACGVKNAPIKANVWANEVAQILGGKGGGRGDFASAGGRDIENLQAALNLAKNT
CCCEEEEEECCCCCCEEECCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
ALKALEG
HHHHCCC
>Mature Secondary Structure
MDIRNEFLQFFHNKGHAVYPSMPLVPNDATLLFTNAGMVQFKDIFTGIVPRPSIPRATSS
CCHHHHHHHHHHCCCCEECCCCCCCCCCCEEEEECCCCHHHHHHHHCCCCCCCCCCCCHH
QLCMRAGGKHNDLENVGYTARHHTLFEMLGNFSFGDYFKEEAILFAWEFVTKNLGFKSKD
HHHHHCCCCCCCHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
LYISVHEKDDEAVKLWEKFVPFERIKKMGDKDNFWQMGDSGPCGPCSEIYIDQGEKHFKG
EEEEEECCCHHHHHHHHHHCCHHHHHHCCCCCCCEECCCCCCCCCCCHHHCCCCCHHCCC
SEDYFGGEGDRFLEIWNLVFMQYERSNDGVLSPLPKPSIDTGMGLERVQALLEHKLNNFD
CCCCCCCCCHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
SSLFAPLMEEISDLASLDYASEFQPSFRVVADHARAVAFLLAQGVHFNKEGRGYVLRRIL
HHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
RRALRHGYLMGLKEAFLYKVVGVVCEQFSNTHAYLKESKEMVMKECFEEEERFLETLESG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MELFNLSLEHLNENKIFDGKIAFKLYDTFGFPLDLTNDMLRSHGACVDMQGFESCMQEQV
HHHHHHHHHHCCCCCEECCEEEEEEEECCCCCCCCHHHHHHHCCCEEEHHHHHHHHHHHH
KRSKASWKGKQNNADFSAILNAYAPNVFVGYETTECCAKVLGFFDSDFKEITDAKPNQEV
HHHHHHCCCCCCCCHHHHHHHHHCCCEEEECCHHHHHHHHHHHHCCHHHHHHCCCCCCEE
WVLLEKTPFYAEGGGAIGDRGALLKDDEEAAIVLDTKNFFGLNFSLLEIKKALKKGDQVI
EEEEECCCCEECCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHH
AQVSDERLEIAKHHSATHLLQSALREVLGSHVSQAGSLVESKRLRFDFSHPKALNDEELE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHH
KVEDLVNAQIFKHLNSQVEHMPLNQAKDKGALALFSEKYAENVRVVSFKEASIELCGGIH
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCHHHHCCEE
VENTGLIGGFRIVKESGVSSGVRRIEAVCGKAFYQLAKEENKELKNAKIALKNNDVIAGI
ECCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEHHH
NKLKESVKNSQKAPVSVELPVEKIHGVNLVVGVVEQGDIKEMIDRLKSKHERLLAMVFKK
HHHHHHHHCCCCCCEEEECCHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHC
ENERITLACGVKNAPIKANVWANEVAQILGGKGGGRGDFASAGGRDIENLQAALNLAKNT
CCCEEEEEECCCCCCEEECCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
ALKALEG
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA