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The map label for this gene is pnp [H]

Identifier: 188528001

GI number: 188528001

Start: 1238368

End: 1240434

Strand: Reverse

Name: pnp [H]

Synonym: HPSH_06280

Alternate gene names: 188528001

Gene position: 1240434-1238368 (Counterclockwise)

Preceding gene: 188528002

Following gene: 188528000

Centisome position: 77.12

GC content: 40.98

Gene sequence:

>2067_bases
ATGGATTTTATCACCATCACTTCTAGTAACAAAACCGAAGAGTTCGCTCTCAAACAAGTGGCCAAACAAGCCACCAGCTC
TCTTATGTATCGCTTAGGAAAAACCATCATTTTAGCGAGCGTGTGCGTAGAAAGAGAGCCTGTGGGTGAAGATTTTCTGC
CTTTAGTGGTGCAGTTTTTAGAAAAATCTTATGCAGCCGGAAAGATCCCGGGCGGCTTTGTTAAAAGAGAAGGCAGGGCG
CAAGATTTTGAAATCTTAACCTCTAGGCTTATAGACAGGACTTTACGCCCTTTATTCCCTAAAGATTACCGCTACCCCAC
ACAGATCACTTTAATGGTTTTAAGCCATGATGTTGAAAATGACTTGCAAGTTTCTGCTTTAAACGCTGCTTCAGCCGCTC
TCTTTTTATCCCATATCGCTCCCATTAAAAGCGTGAGCGCTTGCAGGATCGCTAGGATTGGTAACGAATTTATCATTAAC
CCTAACACAAGCCTTTTGAATCAATCCAGTTTGGATTTGTTCGTGTCCGGCACAAAAGAGAGTTTGAACATGATAGAAAT
GCGCTCTTTGGGGCAACAATTAAACGCTTTAGAAGAGCCTTTAATGCTAAAAGCTTTAGAATTGGCTCAAAAAAGTTTGA
AAGAAACTTGCACGCTTTATGAAGAGATTTTCACGCCCCACCAAAACGAGCTGCTTTTCAAAGAGAGCCAAGGAATAATT
TTTAATGAAAGGCTGTTAGATTTATTAAAAAATCAGTATTTTGATGAAATCATCAAAGGCATTGAAAGTTCTGCTTTGAG
CGAGCGAGAAAATGTTTTCAATGAAATTGCCAGAAAAATCAGTGAAGCCCACTCAGAATTCAGTTTAAAAGAAATTGAAT
TGTCTTTAGAAAAAGTGAAAAAAACTGAGATAAGGTGCATGATTATTAAGGATAAAATCCGCCCGGATAAGCGCGCGTTA
GAAGAAGTGCGGCCCATTTTGATAGAGAGCGATTTGCTCCCTATGGCGCATAGCTCCATTTTATTCACTAGGGGGCAAAC
GCAAAGCTTAGTGGTAGGGGTTTTAGGCACGGATAATGACGCTCAAACCCATGAGAGTTTGGAGCATAAAGCCCCTATTA
AAGAGCGCTTCATGTTTCATTATAATTTCCCTCCTTTTTGCGTGGGCGAAGCGAGTTCTATTGGCGCGACTTCAAGGCGC
GAATTAGGGCATGGGAATTTGGCTAAAAGAGCCTTAGAAACGAGCATTAAAAATAAAGATCAGGTGATACGATTGGTTTC
TGAGATTTTAGAAAGCAATGGTTCAAGCTCAATGGCGAGCGTGTGCGCAGGCTCTTTAGCCCTTTATGCAAGCGGTGTGG
AAATTTATGATCTAATCGCTGGGGTGGCTATGGGCATGGTGAGCGAAGGGCAAGATCACGCCATTTTAAGCGATATTAGC
GGTTTAGAAGACGCAGAAGGCGATATGGATTTTAAGATTGCCGGGAATTTAGAAGGCATTACGGCCATGCAAATGGATAC
CAAAATGAGCGGTATCCAATTAGAGGTTTTATACCAAGCCTTACTCCAAGCTAAAAAGGCGCGAGAGCATATTTTAAAAA
TCATGCATGAAGCGAAAGAAAAGATCGTGATCAATTTTTCCCATTTGCCCACAACGGAGATTTTTAATGTCGCGCCCGAT
AAAATTGTAGAAATTATCGGTCAAGGGGGGCGTGTGATCAGAGAGATAGTAGAAAAGTTTGAGGTTAAAATTGATTTGAA
TAAACCGAGCGGTGAAGTGAAAATCATGGGGAATAAAGAACGGGTTTTGAAAACGAAGGAATTTATTTTAAACTACTTGC
ATTCTTTGGATCAAGAATTGGAACAATACGCTATTGATGAGGTGTTAGAAGCTCAAGTGAAGAGAATCGTGGATTTTGGG
GCGTTTTTAAGCTTGCCTAAGGGAGGCGAAGGCTTGTTAAGAAAGCAAAACATGGACAGGTGTCAAGTGGTTTTAAAAGA
AGGCGATAGCATCAAATGTAGGGTGATTAGTTTCAATAAGGGTAAAATCGCTTTGGATTTGGCTTAA

Upstream 100 bases:

>100_bases
TGGAGCATCATTATAAAGAACTCAAGCGATTAAGCAATGAAGAAATTGAAAAATACTTGGGCGCCAACAACGCGCCTAAT
TTAAAAAAGGAACATTAAAT

Downstream 100 bases:

>100_bases
AATTTTAAAAAGCGTTTTTTAAAAGAGATTTTAAGCTAGTTTGTATTAAAATAAATTCTTGCTTGTATTTTTGAAAGTAG
AGGAACGATTCGAAATGAAA

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 688; Mature: 688

Protein sequence:

>688_residues
MDFITITSSNKTEEFALKQVAKQATSSLMYRLGKTIILASVCVEREPVGEDFLPLVVQFLEKSYAAGKIPGGFVKREGRA
QDFEILTSRLIDRTLRPLFPKDYRYPTQITLMVLSHDVENDLQVSALNAASAALFLSHIAPIKSVSACRIARIGNEFIIN
PNTSLLNQSSLDLFVSGTKESLNMIEMRSLGQQLNALEEPLMLKALELAQKSLKETCTLYEEIFTPHQNELLFKESQGII
FNERLLDLLKNQYFDEIIKGIESSALSERENVFNEIARKISEAHSEFSLKEIELSLEKVKKTEIRCMIIKDKIRPDKRAL
EEVRPILIESDLLPMAHSSILFTRGQTQSLVVGVLGTDNDAQTHESLEHKAPIKERFMFHYNFPPFCVGEASSIGATSRR
ELGHGNLAKRALETSIKNKDQVIRLVSEILESNGSSSMASVCAGSLALYASGVEIYDLIAGVAMGMVSEGQDHAILSDIS
GLEDAEGDMDFKIAGNLEGITAMQMDTKMSGIQLEVLYQALLQAKKAREHILKIMHEAKEKIVINFSHLPTTEIFNVAPD
KIVEIIGQGGRVIREIVEKFEVKIDLNKPSGEVKIMGNKERVLKTKEFILNYLHSLDQELEQYAIDEVLEAQVKRIVDFG
AFLSLPKGGEGLLRKQNMDRCQVVLKEGDSIKCRVISFNKGKIALDLA

Sequences:

>Translated_688_residues
MDFITITSSNKTEEFALKQVAKQATSSLMYRLGKTIILASVCVEREPVGEDFLPLVVQFLEKSYAAGKIPGGFVKREGRA
QDFEILTSRLIDRTLRPLFPKDYRYPTQITLMVLSHDVENDLQVSALNAASAALFLSHIAPIKSVSACRIARIGNEFIIN
PNTSLLNQSSLDLFVSGTKESLNMIEMRSLGQQLNALEEPLMLKALELAQKSLKETCTLYEEIFTPHQNELLFKESQGII
FNERLLDLLKNQYFDEIIKGIESSALSERENVFNEIARKISEAHSEFSLKEIELSLEKVKKTEIRCMIIKDKIRPDKRAL
EEVRPILIESDLLPMAHSSILFTRGQTQSLVVGVLGTDNDAQTHESLEHKAPIKERFMFHYNFPPFCVGEASSIGATSRR
ELGHGNLAKRALETSIKNKDQVIRLVSEILESNGSSSMASVCAGSLALYASGVEIYDLIAGVAMGMVSEGQDHAILSDIS
GLEDAEGDMDFKIAGNLEGITAMQMDTKMSGIQLEVLYQALLQAKKAREHILKIMHEAKEKIVINFSHLPTTEIFNVAPD
KIVEIIGQGGRVIREIVEKFEVKIDLNKPSGEVKIMGNKERVLKTKEFILNYLHSLDQELEQYAIDEVLEAQVKRIVDFG
AFLSLPKGGEGLLRKQNMDRCQVVLKEGDSIKCRVISFNKGKIALDLA
>Mature_688_residues
MDFITITSSNKTEEFALKQVAKQATSSLMYRLGKTIILASVCVEREPVGEDFLPLVVQFLEKSYAAGKIPGGFVKREGRA
QDFEILTSRLIDRTLRPLFPKDYRYPTQITLMVLSHDVENDLQVSALNAASAALFLSHIAPIKSVSACRIARIGNEFIIN
PNTSLLNQSSLDLFVSGTKESLNMIEMRSLGQQLNALEEPLMLKALELAQKSLKETCTLYEEIFTPHQNELLFKESQGII
FNERLLDLLKNQYFDEIIKGIESSALSERENVFNEIARKISEAHSEFSLKEIELSLEKVKKTEIRCMIIKDKIRPDKRAL
EEVRPILIESDLLPMAHSSILFTRGQTQSLVVGVLGTDNDAQTHESLEHKAPIKERFMFHYNFPPFCVGEASSIGATSRR
ELGHGNLAKRALETSIKNKDQVIRLVSEILESNGSSSMASVCAGSLALYASGVEIYDLIAGVAMGMVSEGQDHAILSDIS
GLEDAEGDMDFKIAGNLEGITAMQMDTKMSGIQLEVLYQALLQAKKAREHILKIMHEAKEKIVINFSHLPTTEIFNVAPD
KIVEIIGQGGRVIREIVEKFEVKIDLNKPSGEVKIMGNKERVLKTKEFILNYLHSLDQELEQYAIDEVLEAQVKRIVDFG
AFLSLPKGGEGLLRKQNMDRCQVVLKEGDSIKCRVISFNKGKIALDLA

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=691, Percent_Identity=32.4167872648336, Blast_Score=306, Evalue=4e-83,
Organism=Escherichia coli, GI145693187, Length=690, Percent_Identity=36.3768115942029, Blast_Score=397, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI115534063, Length=661, Percent_Identity=30.4084720121029, Blast_Score=270, Evalue=2e-72,
Organism=Drosophila melanogaster, GI281362905, Length=597, Percent_Identity=35.1758793969849, Blast_Score=310, Evalue=3e-84,
Organism=Drosophila melanogaster, GI24651641, Length=597, Percent_Identity=35.1758793969849, Blast_Score=310, Evalue=3e-84,
Organism=Drosophila melanogaster, GI24651643, Length=597, Percent_Identity=35.1758793969849, Blast_Score=310, Evalue=3e-84,
Organism=Drosophila melanogaster, GI161079377, Length=541, Percent_Identity=36.0443622920518, Blast_Score=285, Evalue=5e-77,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 76822; Mature: 76822

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFITITSSNKTEEFALKQVAKQATSSLMYRLGKTIILASVCVEREPVGEDFLPLVVQFL
CCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
EKSYAAGKIPGGFVKREGRAQDFEILTSRLIDRTLRPLFPKDYRYPTQITLMVLSHDVEN
HHHCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCC
DLQVSALNAASAALFLSHIAPIKSVSACRIARIGNEFIINPNTSLLNQSSLDLFVSGTKE
CCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHCCCCCCEEEEECCHH
SLNMIEMRSLGQQLNALEEPLMLKALELAQKSLKETCTLYEEIFTPHQNELLFKESQGII
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCE
FNERLLDLLKNQYFDEIIKGIESSALSERENVFNEIARKISEAHSEFSLKEIELSLEKVK
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
KTEIRCMIIKDKIRPDKRALEEVRPILIESDLLPMAHSSILFTRGQTQSLVVGVLGTDND
HHCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCHHCCCEEEECCCCCEEEEEEEECCCC
AQTHESLEHKAPIKERFMFHYNFPPFCVGEASSIGATSRRELGHGNLAKRALETSIKNKD
HHHHHHHHHCCCHHHCEEEEECCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHCCHH
QVIRLVSEILESNGSSSMASVCAGSLALYASGVEIYDLIAGVAMGMVSEGQDHAILSDIS
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHC
GLEDAEGDMDFKIAGNLEGITAMQMDTKMSGIQLEVLYQALLQAKKAREHILKIMHEAKE
CCCCCCCCCCEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KIVINFSHLPTTEIFNVAPDKIVEIIGQGGRVIREIVEKFEVKIDLNKPSGEVKIMGNKE
EEEEEECCCCCHHHHCCCHHHHHHHHCCCCHHHHHHHHHHEEEEEECCCCCCEEEECCHH
RVLKTKEFILNYLHSLDQELEQYAIDEVLEAQVKRIVDFGAFLSLPKGGEGLLRKQNMDR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCHHH
CQVVLKEGDSIKCRVISFNKGKIALDLA
HHHHHHCCCCEEEEEEEECCCEEEEEEC
>Mature Secondary Structure
MDFITITSSNKTEEFALKQVAKQATSSLMYRLGKTIILASVCVEREPVGEDFLPLVVQFL
CCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
EKSYAAGKIPGGFVKREGRAQDFEILTSRLIDRTLRPLFPKDYRYPTQITLMVLSHDVEN
HHHCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCC
DLQVSALNAASAALFLSHIAPIKSVSACRIARIGNEFIINPNTSLLNQSSLDLFVSGTKE
CCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHCCCCCCEEEEECCHH
SLNMIEMRSLGQQLNALEEPLMLKALELAQKSLKETCTLYEEIFTPHQNELLFKESQGII
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCE
FNERLLDLLKNQYFDEIIKGIESSALSERENVFNEIARKISEAHSEFSLKEIELSLEKVK
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
KTEIRCMIIKDKIRPDKRALEEVRPILIESDLLPMAHSSILFTRGQTQSLVVGVLGTDND
HHCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCHHCCCEEEECCCCCEEEEEEEECCCC
AQTHESLEHKAPIKERFMFHYNFPPFCVGEASSIGATSRRELGHGNLAKRALETSIKNKD
HHHHHHHHHCCCHHHCEEEEECCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHCCHH
QVIRLVSEILESNGSSSMASVCAGSLALYASGVEIYDLIAGVAMGMVSEGQDHAILSDIS
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHC
GLEDAEGDMDFKIAGNLEGITAMQMDTKMSGIQLEVLYQALLQAKKAREHILKIMHEAKE
CCCCCCCCCCEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KIVINFSHLPTTEIFNVAPDKIVEIIGQGGRVIREIVEKFEVKIDLNKPSGEVKIMGNKE
EEEEEECCCCCHHHHCCCHHHHHHHHCCCCHHHHHHHHHHEEEEEECCCCCCEEEECCHH
RVLKTKEFILNYLHSLDQELEQYAIDEVLEAQVKRIVDFGAFLSLPKGGEGLLRKQNMDR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCHHH
CQVVLKEGDSIKCRVISFNKGKIALDLA
HHHHHHCCCCEEEEEEEECCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA