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The map label for this gene is purB [H]

Identifier: 188527892

GI number: 188527892

Start: 1127704

End: 1129026

Strand: Direct

Name: purB [H]

Synonym: HPSH_05715

Alternate gene names: 188527892

Gene position: 1127704-1129026 (Clockwise)

Preceding gene: 188527891

Following gene: 188527893

Centisome position: 70.11

GC content: 41.5

Gene sequence:

>1323_bases
GTGTTAGAACGCTATGCGAATGAAGAAATGAAAGCCCTATGGAATGAGCAAACCAAGTTTGAAACCTATTTAGAAGTGGA
AAAAGCTGTCGTTAGGGCGTGGAACAAGCTTGGGCAAATCCAAGATAGCGATTGTGAAAAAATCTGCTCAAAGGCGGCAT
TCAACCTTGAGCGCATCAAAGAAATTGAAAAAACCACTAAGCATGACTTAATCGCTTTCACCACTTGCGTGGCTGAAAGC
CTGGGCGAAGAATCGCGCTTTTTTCATTATGGGATCACTTCTAGCGATTGCATTGATACGGCTATGGCACTATTGATGAC
AAAAAGCTTAAAACTCATTCAAAAAGGCGTTAAAAACCTCTATGAAACCCTTAAAAACAGGGCTTTAGAGCATAAAGACA
CGCTGATGGTGGGCAGAAGCCATGGGGTGTTTGGCGAACCCATTACTTTTGGTTTAGTGTTAGCCCTTTTTGCTGATGAA
ATCAAACGGCATTTAAAAGCCTTAGATTTAACGATGGAATTTATCAGCGTGGGAGCGATCAGTGGGGCTATGGGGAATTT
CGCGCACGCCCCCTTAGAATTAGAAGAATTAGCATGCAAATTTTTAGGCTTAAAAACCGCCAATATCAATAATCAAGTCA
TTCAAAGGGATCGCTACGCTAGGCTTGCATGCGATCTGGCTCTTTTAGCGAGCAGCTGTGAAAAAATCGCTGTCAATATC
CGCCATTTGCAACGCAGTGAAGTCTATGAAGTGGAAGAAGCTTTTTCAACAGGGCAAAAAGGAAGCTCGGCGATGCCTCA
TAAAAGAAACCCTATATTGAGCGAGAATATCACCGGGCTTTGTAGGGTGATTCGCTCTTTTACTACCCCTATGCTAGAAA
ATGTCGCCTTATGGCATGAAAGGGACATGAGCCATAGCTCTGTGGAGCGTTTTGCATTACCCGATCTGTTTGTTACTAGC
GATTTTATGCTCAGCCGCTTGAATAGCGTGATTGAAAATTTGGTGGTTTATCCTAAAAACATGCTTAAAAATTTAGCTTT
GAGTGGGGGGCTAGTCTTTTCGCAACGGGTGTTATTGGAATTGCCTAAAAAAGGTTTGAGCCGAGAAGAAAGCTATTCTA
TCGTGCAAGAAAATGCGATGAAAATATGGGAGGTTTTGCAACAAGGCGCTTTTAAAAACGCTGATGAAAATTTGTTTTTA
AACGCCCTACTCAACGATGAACGCTTGAAAAAATATTTGAGCGAGGATGAAATCAAAGCATGTTTTGATTATAGTTATTA
CACTAAAAATGTGGGGGCGATTTTTAAAAGGGTGTTTGAATAG

Upstream 100 bases:

>100_bases
CAAATCAAGGCGCTTTTTGGCGTCTTGTGTGATTTTCCCCCTTGACTTTTTAAACCCCTTTAAAGTAACATAAATCCCAT
TTTGATTGTAAGGATTGTCG

Downstream 100 bases:

>100_bases
GGTGCGTTTTCACAAAAACAAAACTACAATAAGCGCTAAAAAATTAAAGTTTTAAATTAAGGGGTTTTTTGAAACGAGCG
TTATGTTTGATTTTAGGGCT

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MLERYANEEMKALWNEQTKFETYLEVEKAVVRAWNKLGQIQDSDCEKICSKAAFNLERIKEIEKTTKHDLIAFTTCVAES
LGEESRFFHYGITSSDCIDTAMALLMTKSLKLIQKGVKNLYETLKNRALEHKDTLMVGRSHGVFGEPITFGLVLALFADE
IKRHLKALDLTMEFISVGAISGAMGNFAHAPLELEELACKFLGLKTANINNQVIQRDRYARLACDLALLASSCEKIAVNI
RHLQRSEVYEVEEAFSTGQKGSSAMPHKRNPILSENITGLCRVIRSFTTPMLENVALWHERDMSHSSVERFALPDLFVTS
DFMLSRLNSVIENLVVYPKNMLKNLALSGGLVFSQRVLLELPKKGLSREESYSIVQENAMKIWEVLQQGAFKNADENLFL
NALLNDERLKKYLSEDEIKACFDYSYYTKNVGAIFKRVFE

Sequences:

>Translated_440_residues
MLERYANEEMKALWNEQTKFETYLEVEKAVVRAWNKLGQIQDSDCEKICSKAAFNLERIKEIEKTTKHDLIAFTTCVAES
LGEESRFFHYGITSSDCIDTAMALLMTKSLKLIQKGVKNLYETLKNRALEHKDTLMVGRSHGVFGEPITFGLVLALFADE
IKRHLKALDLTMEFISVGAISGAMGNFAHAPLELEELACKFLGLKTANINNQVIQRDRYARLACDLALLASSCEKIAVNI
RHLQRSEVYEVEEAFSTGQKGSSAMPHKRNPILSENITGLCRVIRSFTTPMLENVALWHERDMSHSSVERFALPDLFVTS
DFMLSRLNSVIENLVVYPKNMLKNLALSGGLVFSQRVLLELPKKGLSREESYSIVQENAMKIWEVLQQGAFKNADENLFL
NALLNDERLKKYLSEDEIKACFDYSYYTKNVGAIFKRVFE
>Mature_440_residues
MLERYANEEMKALWNEQTKFETYLEVEKAVVRAWNKLGQIQDSDCEKICSKAAFNLERIKEIEKTTKHDLIAFTTCVAES
LGEESRFFHYGITSSDCIDTAMALLMTKSLKLIQKGVKNLYETLKNRALEHKDTLMVGRSHGVFGEPITFGLVLALFADE
IKRHLKALDLTMEFISVGAISGAMGNFAHAPLELEELACKFLGLKTANINNQVIQRDRYARLACDLALLASSCEKIAVNI
RHLQRSEVYEVEEAFSTGQKGSSAMPHKRNPILSENITGLCRVIRSFTTPMLENVALWHERDMSHSSVERFALPDLFVTS
DFMLSRLNSVIENLVVYPKNMLKNLALSGGLVFSQRVLLELPKKGLSREESYSIVQENAMKIWEVLQQGAFKNADENLFL
NALLNDERLKKYLSEDEIKACFDYSYYTKNVGAIFKRVFE

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=457, Percent_Identity=25.601750547046, Blast_Score=122, Evalue=8e-28,
Organism=Homo sapiens, GI183227688, Length=406, Percent_Identity=27.0935960591133, Blast_Score=121, Evalue=1e-27,
Organism=Escherichia coli, GI1787376, Length=279, Percent_Identity=28.3154121863799, Blast_Score=81, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17508577, Length=355, Percent_Identity=26.1971830985915, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI32564234, Length=320, Percent_Identity=27.1875, Blast_Score=92, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6323391, Length=386, Percent_Identity=26.1658031088083, Blast_Score=130, Evalue=5e-31,
Organism=Drosophila melanogaster, GI24647570, Length=380, Percent_Identity=26.3157894736842, Blast_Score=117, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 50001; Mature: 50001

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLERYANEEMKALWNEQTKFETYLEVEKAVVRAWNKLGQIQDSDCEKICSKAAFNLERIK
CCCHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCHHHHH
EIEKTTKHDLIAFTTCVAESLGEESRFFHYGITSSDCIDTAMALLMTKSLKLIQKGVKNL
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
YETLKNRALEHKDTLMVGRSHGVFGEPITFGLVLALFADEIKRHLKALDLTMEFISVGAI
HHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGAMGNFAHAPLELEELACKFLGLKTANINNQVIQRDRYARLACDLALLASSCEKIAVNI
HHHHCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RHLQRSEVYEVEEAFSTGQKGSSAMPHKRNPILSENITGLCRVIRSFTTPMLENVALWHE
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDMSHSSVERFALPDLFVTSDFMLSRLNSVIENLVVYPKNMLKNLALSGGLVFSQRVLLE
CCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHH
LPKKGLSREESYSIVQENAMKIWEVLQQGAFKNADENLFLNALLNDERLKKYLSEDEIKA
HCHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCHHHHHHHHCHHHHHH
CFDYSYYTKNVGAIFKRVFE
HHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLERYANEEMKALWNEQTKFETYLEVEKAVVRAWNKLGQIQDSDCEKICSKAAFNLERIK
CCCHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCHHHHH
EIEKTTKHDLIAFTTCVAESLGEESRFFHYGITSSDCIDTAMALLMTKSLKLIQKGVKNL
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
YETLKNRALEHKDTLMVGRSHGVFGEPITFGLVLALFADEIKRHLKALDLTMEFISVGAI
HHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGAMGNFAHAPLELEELACKFLGLKTANINNQVIQRDRYARLACDLALLASSCEKIAVNI
HHHHCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RHLQRSEVYEVEEAFSTGQKGSSAMPHKRNPILSENITGLCRVIRSFTTPMLENVALWHE
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDMSHSSVERFALPDLFVTSDFMLSRLNSVIENLVVYPKNMLKNLALSGGLVFSQRVLLE
CCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHH
LPKKGLSREESYSIVQENAMKIWEVLQQGAFKNADENLFLNALLNDERLKKYLSEDEIKA
HCHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCHHHHHHHHCHHHHHH
CFDYSYYTKNVGAIFKRVFE
HHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]