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The map label for this gene is ispH

Identifier: 188527835

GI number: 188527835

Start: 1066674

End: 1067498

Strand: Direct

Name: ispH

Synonym: HPSH_05405

Alternate gene names: 188527835

Gene position: 1066674-1067498 (Clockwise)

Preceding gene: 188527834

Following gene: 188527836

Centisome position: 66.31

GC content: 39.39

Gene sequence:

>825_bases
ATGGAAATTAAAATGGCTAAGGATTATGGTTTTTGTTTTGGCGTCAAAAGAGCGATACAAATCGCTGAAAAAAATCAAAA
CAGCTTGATTTTTGGCTCGCTCATTCATAACGCTAAAGAAATCAATCGTTTGGAAAAAAACTTCAATGTGAAAATTGAAG
AAGACCCTAAAAAAATCCCTAAAAATAAGAGCGTGATCATAAGAACCCACGGCATTCCTAAGCAGGATTTAGAATACTTG
AAAAATAAGGGGGTCAAAATCACTGATGCGACTTGCCCGTATGTGATCAAACCCCAGCAAATCGTGGAATCCATGAGCAA
AGAAGGGTATCAAATCGTGCTTTTTGGGGATATTAACCACCCTGAAGTCAAGGGCGTGATCAGCTATGCCACTAACCAGG
CTTTAGTCATCAATTCGTTAGAAGAATTGCAAGAAAAAAAGCTCCAACGAAAAGTGGCTTTAGTCTCCCAAACCACCAAA
CAAACCCCCCAACTCTTGCAAATCGCTTCTTACTTGGTGGAAAGATGCACTGAAGTGCGTATTTTTAACACGATTTGTAA
CGCAACTTCTTACAACCAAAAAGCCGCTTTGGATTTGAGTAAGGAAGTGGATATTATGATAGTCGTAGGCGGTAAAACTT
CTTCAAACACCAAACAGCTCTTAAGTATCGCCAAACAGCATTGTGAAGACAGCTACTTGGTAGAAGATGAAAACGAATTA
GAGTTAGCGTGGTTTAAGAATAAAAAATTGTGTGGGATTACCGCTGGGGCTTCCACGCCGGACTGGATTATAGAAAATGT
CAAGCAAAAAATCAGCACGATTTAA

Upstream 100 bases:

>100_bases
ACTTTAGCATTGCCTTTAGAAATTGACAATTTAGAATGCGCAAACATTTCTTTCCCGCAATTCAAACGCTTACTCAATCT
ATTCAAAAAAAGGAGTTTTA

Downstream 100 bases:

>100_bases
CACATTTTGAAAATTTTCTTTAAAAAAACCGCTAATTGTTAGTTTTTAAACGATTTTGTAAGCCAAAAAAGGATACAATG
CCCCTAAGGATTTTATATTT

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MEIKMAKDYGFCFGVKRAIQIAEKNQNSLIFGSLIHNAKEINRLEKNFNVKIEEDPKKIPKNKSVIIRTHGIPKQDLEYL
KNKGVKITDATCPYVIKPQQIVESMSKEGYQIVLFGDINHPEVKGVISYATNQALVINSLEELQEKKLQRKVALVSQTTK
QTPQLLQIASYLVERCTEVRIFNTICNATSYNQKAALDLSKEVDIMIVVGGKTSSNTKQLLSIAKQHCEDSYLVEDENEL
ELAWFKNKKLCGITAGASTPDWIIENVKQKISTI

Sequences:

>Translated_274_residues
MEIKMAKDYGFCFGVKRAIQIAEKNQNSLIFGSLIHNAKEINRLEKNFNVKIEEDPKKIPKNKSVIIRTHGIPKQDLEYL
KNKGVKITDATCPYVIKPQQIVESMSKEGYQIVLFGDINHPEVKGVISYATNQALVINSLEELQEKKLQRKVALVSQTTK
QTPQLLQIASYLVERCTEVRIFNTICNATSYNQKAALDLSKEVDIMIVVGGKTSSNTKQLLSIAKQHCEDSYLVEDENEL
ELAWFKNKKLCGITAGASTPDWIIENVKQKISTI
>Mature_274_residues
MEIKMAKDYGFCFGVKRAIQIAEKNQNSLIFGSLIHNAKEINRLEKNFNVKIEEDPKKIPKNKSVIIRTHGIPKQDLEYL
KNKGVKITDATCPYVIKPQQIVESMSKEGYQIVLFGDINHPEVKGVISYATNQALVINSLEELQEKKLQRKVALVSQTTK
QTPQLLQIASYLVERCTEVRIFNTICNATSYNQKAALDLSKEVDIMIVVGGKTSSNTKQLLSIAKQHCEDSYLVEDENEL
ELAWFKNKKLCGITAGASTPDWIIENVKQKISTI

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) [H]

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family [H]

Homologues:

Organism=Escherichia coli, GI1786212, Length=286, Percent_Identity=29.3706293706294, Blast_Score=132, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003451 [H]

Pfam domain/function: PF02401 LYTB [H]

EC number: =1.17.1.2 [H]

Molecular weight: Translated: 30931; Mature: 30931

Theoretical pI: Translated: 8.93; Mature: 8.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIKMAKDYGFCFGVKRAIQIAEKNQNSLIFGSLIHNAKEINRLEKNFNVKIEEDPKKIP
CCEEECCCCCHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCCCEEECCCHHHCC
KNKSVIIRTHGIPKQDLEYLKNKGVKITDATCPYVIKPQQIVESMSKEGYQIVLFGDINH
CCCEEEEEECCCCHHHHHHHHHCCCEEEECCCCEEECHHHHHHHHHHCCCEEEEEECCCC
PEVKGVISYATNQALVINSLEELQEKKLQRKVALVSQTTKQTPQLLQIASYLVERCTEVR
CHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
IFNTICNATSYNQKAALDLSKEVDIMIVVGGKTSSNTKQLLSIAKQHCEDSYLVEDENEL
HHHHHHCCCCCCCHHEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCEEECCCCCE
ELAWFKNKKLCGITAGASTPDWIIENVKQKISTI
EEEEECCCCEEEEECCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEIKMAKDYGFCFGVKRAIQIAEKNQNSLIFGSLIHNAKEINRLEKNFNVKIEEDPKKIP
CCEEECCCCCHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCCCEEECCCHHHCC
KNKSVIIRTHGIPKQDLEYLKNKGVKITDATCPYVIKPQQIVESMSKEGYQIVLFGDINH
CCCEEEEEECCCCHHHHHHHHHCCCEEEECCCCEEECHHHHHHHHHHCCCEEEEEECCCC
PEVKGVISYATNQALVINSLEELQEKKLQRKVALVSQTTKQTPQLLQIASYLVERCTEVR
CHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
IFNTICNATSYNQKAALDLSKEVDIMIVVGGKTSSNTKQLLSIAKQHCEDSYLVEDENEL
HHHHHHCCCCCCCHHEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCEEECCCCCE
ELAWFKNKKLCGITAGASTPDWIIENVKQKISTI
EEEEECCCCEEEEECCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA