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The map label for this gene is proP [H]

Identifier: 188527759

GI number: 188527759

Start: 979596

End: 980765

Strand: Reverse

Name: proP [H]

Synonym: HPSH_04930

Alternate gene names: 188527759

Gene position: 980765-979596 (Counterclockwise)

Preceding gene: 188527760

Following gene: 188527747

Centisome position: 60.97

GC content: 37.69

Gene sequence:

>1170_bases
ATGGCCCACTTTGGGGATAAATTCGGCCGTAAAAACATGTTCATGCTCTCTATTTTATTGATGGTAATCCCAACCTTTGC
ACTCGCTTTGATGCCAACTTTTAATCATTTTGTGAGTTTTGGCGTTGATAGCATGGGACTTACCCCAAAAAACGCTCATT
ATCTTGGTTACATAGCTCCTGTTTTTTTGATATTTGTTAGGATCTGTCAAGGCGTTGCTGTGGGTGGTGAATTGCCTGGT
GCTTGGGTTTTTGTTTATGAACATGCTCCGCAAGGTCAAAAAAACACTTATATTGGTTTTTTAAGCGCTTCTGTAGTTTC
TGGGATTTTGCTTGGGAGTTTGGTTTATATTGGGATTTACATGGTTTTTGACAAGCCTGTTGTTGAAGCTTGGGCTTGGC
GGGTTGCTTTTGGGCTTGGAGGGATTTTTGGTATCGTTTCTGTCTATTTGAGACGCTTTTTAGAAGAAACTCCTGTTTTT
CAGCAAATGAAGCAAGACAATGCATTAGTTAAATTCCCGCTTAAAGAGGTGTTTAAGAATTCTTTCTTTGGTATATTAAT
CTCCATGCTTATCACTTGGGTTTTAACCGCTTGTATTTTGATTTTTATCCTTTTTGTTCCAAATTTTACCCTCATGCATC
CCAATTTTAATTTCACTCCTTTTGAAAAAACCTATTTTCAAATTTTAGGGCTTGTTGGCATTGTAAGTTCTATTATTTTA
ACCGGGTTTTTGGCCGATAAAATCAAACCGCACAAAGTTTGCATGGCTTTTAGCACGGCCTTTGCCTTTTTTGGCTTTTT
ATTCTTTAAAGAATTTTATTCTAACGCGCCGAGTTTAGTGAATACCATAGTTTTATACTTTTTAGCTTGCTTTTGTGCGG
GTATTATGAATTTTTGCCCTATTTTTATGAGCGATGTGTTTAGTGCTAGAATCCGTTTTAGCGGGATTTCCTTCGCTTAT
AACATAGCCTATGCTATAACCGCTGGCTTTACCCCTCAACTTTCAAGCTGGTTAAATGCAAAAGCTATAGCCGCACCTGA
AAGTTTGCAAAGTTATGGTTTAAGCTTTTATATTTTTGTGGTTGCTTTAATTGCTTTTATCGTATCGCTTTTAATGGCGC
CAATTTACAATCAATCTAACCCCCAACACGAAGTGCCGCCCATAACATGA

Upstream 100 bases:

>100_bases
ACACTTTTTCCCAAACACTCTCAGCCCTATTTGGTCTGAAATCAACACTTATGGTATCTTTGCTGCAGGTTATCTGGCAC
GCCCGCTTGGCGGCATAGTG

Downstream 100 bases:

>100_bases
AAGAAGCGCAAACCTTTAGGTAATTCAATCAAGATTTAGCCGATAGTGTTGGTGGGTTTTTAGTGGTGATGAAATCCTTA
ACCCCGCGCATCACAATAAA

Product: proline and betaine transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 389; Mature: 388

Protein sequence:

>389_residues
MAHFGDKFGRKNMFMLSILLMVIPTFALALMPTFNHFVSFGVDSMGLTPKNAHYLGYIAPVFLIFVRICQGVAVGGELPG
AWVFVYEHAPQGQKNTYIGFLSASVVSGILLGSLVYIGIYMVFDKPVVEAWAWRVAFGLGGIFGIVSVYLRRFLEETPVF
QQMKQDNALVKFPLKEVFKNSFFGILISMLITWVLTACILIFILFVPNFTLMHPNFNFTPFEKTYFQILGLVGIVSSIIL
TGFLADKIKPHKVCMAFSTAFAFFGFLFFKEFYSNAPSLVNTIVLYFLACFCAGIMNFCPIFMSDVFSARIRFSGISFAY
NIAYAITAGFTPQLSSWLNAKAIAAPESLQSYGLSFYIFVVALIAFIVSLLMAPIYNQSNPQHEVPPIT

Sequences:

>Translated_389_residues
MAHFGDKFGRKNMFMLSILLMVIPTFALALMPTFNHFVSFGVDSMGLTPKNAHYLGYIAPVFLIFVRICQGVAVGGELPG
AWVFVYEHAPQGQKNTYIGFLSASVVSGILLGSLVYIGIYMVFDKPVVEAWAWRVAFGLGGIFGIVSVYLRRFLEETPVF
QQMKQDNALVKFPLKEVFKNSFFGILISMLITWVLTACILIFILFVPNFTLMHPNFNFTPFEKTYFQILGLVGIVSSIIL
TGFLADKIKPHKVCMAFSTAFAFFGFLFFKEFYSNAPSLVNTIVLYFLACFCAGIMNFCPIFMSDVFSARIRFSGISFAY
NIAYAITAGFTPQLSSWLNAKAIAAPESLQSYGLSFYIFVVALIAFIVSLLMAPIYNQSNPQHEVPPIT
>Mature_388_residues
AHFGDKFGRKNMFMLSILLMVIPTFALALMPTFNHFVSFGVDSMGLTPKNAHYLGYIAPVFLIFVRICQGVAVGGELPGA
WVFVYEHAPQGQKNTYIGFLSASVVSGILLGSLVYIGIYMVFDKPVVEAWAWRVAFGLGGIFGIVSVYLRRFLEETPVFQ
QMKQDNALVKFPLKEVFKNSFFGILISMLITWVLTACILIFILFVPNFTLMHPNFNFTPFEKTYFQILGLVGIVSSIILT
GFLADKIKPHKVCMAFSTAFAFFGFLFFKEFYSNAPSLVNTIVLYFLACFCAGIMNFCPIFMSDVFSARIRFSGISFAYN
IAYAITAGFTPQLSSWLNAKAIAAPESLQSYGLSFYIFVVALIAFIVSLLMAPIYNQSNPQHEVPPIT

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=390, Percent_Identity=26.1538461538462, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1788292, Length=381, Percent_Identity=26.246719160105, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1789941, Length=387, Percent_Identity=23.5142118863049, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1788942, Length=386, Percent_Identity=24.6113989637306, Blast_Score=82, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 43415; Mature: 43284

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHFGDKFGRKNMFMLSILLMVIPTFALALMPTFNHFVSFGVDSMGLTPKNAHYLGYIAP
CCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
VFLIFVRICQGVAVGGELPGAWVFVYEHAPQGQKNTYIGFLSASVVSGILLGSLVYIGIY
HHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
MVFDKPVVEAWAWRVAFGLGGIFGIVSVYLRRFLEETPVFQQMKQDNALVKFPLKEVFKN
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCEEECCHHHHHHH
SFFGILISMLITWVLTACILIFILFVPNFTLMHPNFNFTPFEKTYFQILGLVGIVSSIIL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHH
TGFLADKIKPHKVCMAFSTAFAFFGFLFFKEFYSNAPSLVNTIVLYFLACFCAGIMNFCP
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
IFMSDVFSARIRFSGISFAYNIAYAITAGFTPQLSSWLNAKAIAAPESLQSYGLSFYIFV
HHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHCCHHHCCHHHHHHHCHHHHHHH
VALIAFIVSLLMAPIYNQSNPQHEVPPIT
HHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
AHFGDKFGRKNMFMLSILLMVIPTFALALMPTFNHFVSFGVDSMGLTPKNAHYLGYIAP
CCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
VFLIFVRICQGVAVGGELPGAWVFVYEHAPQGQKNTYIGFLSASVVSGILLGSLVYIGIY
HHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
MVFDKPVVEAWAWRVAFGLGGIFGIVSVYLRRFLEETPVFQQMKQDNALVKFPLKEVFKN
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCEEECCHHHHHHH
SFFGILISMLITWVLTACILIFILFVPNFTLMHPNFNFTPFEKTYFQILGLVGIVSSIIL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHH
TGFLADKIKPHKVCMAFSTAFAFFGFLFFKEFYSNAPSLVNTIVLYFLACFCAGIMNFCP
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
IFMSDVFSARIRFSGISFAYNIAYAITAGFTPQLSSWLNAKAIAAPESLQSYGLSFYIFV
HHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHCCHHHCCHHHHHHHCHHHHHHH
VALIAFIVSLLMAPIYNQSNPQHEVPPIT
HHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]