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The map label for this gene is folE

Identifier: 188527751

GI number: 188527751

Start: 972473

End: 973015

Strand: Direct

Name: folE

Synonym: HPSH_04890

Alternate gene names: 188527751

Gene position: 972473-973015 (Clockwise)

Preceding gene: 188527750

Following gene: 188527752

Centisome position: 60.46

GC content: 37.75

Gene sequence:

>543_bases
ATGGAAAATTTTTTCAACCAGTTTTTTGAAAATATCGGCGAAGATAAAAACAGAGAAGGTTTGAAAGAGACGCCTAAAAG
GGTTCAAGAATTATGGAAATTCTTGTATAAAGGCTATAAAGAAGATCCTAAAGTGGCTTTAAAAAGCGCGTATTTTCAAG
GCGTTTGCGATGAAATGATAGTGGCTCAAAACATTGAATTTTACTCCACTTGCGAACACCACTTGCTCCCTTTTTTGGGG
AATATCAGTTTAGGATACATTCCTAAAGAAAAGATTGTAGGCATTAGTGCGATCGCTAAACTCATTGAAATTTATAGCAG
ACGCTTGCAAATCCAAGAAAGGCTGACCACTCAAATTGCCGAAACCTTTGATGAAATCATAGAGCCAAGGGGCGTGATTG
TGGTTTGTGAAGCCAAGCATTTGTGCATGAGCATGCAAGGGGTGCAAAAGCAAAATGCGATCATTAAAACAAGCGTTCTA
AAAGGCCTCTTTAAAAAAGACCCTAAAACCAGAGCTGAATTTATGCAACTCTTAAAATCTTAG

Upstream 100 bases:

>100_bases
CTCCACTCGATCGGCAGCTTATCTTTTTAGCGCTGAAATGTTTAGCACCCACCCTAGCATTAAAAATCGTATCCAATCTT
TATCAAGGCGCGTCATTTAA

Downstream 100 bases:

>100_bases
GTTATAATTCTGTGCATGAATAACCCTAATTTATCCTTTTATTATAATGAGTGCGAGCGTTTTGAAAGCTTTTTAAACCA
CCATCATTTACACCTTGAAA

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I [H]

Number of amino acids: Translated: 180; Mature: 180

Protein sequence:

>180_residues
MENFFNQFFENIGEDKNREGLKETPKRVQELWKFLYKGYKEDPKVALKSAYFQGVCDEMIVAQNIEFYSTCEHHLLPFLG
NISLGYIPKEKIVGISAIAKLIEIYSRRLQIQERLTTQIAETFDEIIEPRGVIVVCEAKHLCMSMQGVQKQNAIIKTSVL
KGLFKKDPKTRAEFMQLLKS

Sequences:

>Translated_180_residues
MENFFNQFFENIGEDKNREGLKETPKRVQELWKFLYKGYKEDPKVALKSAYFQGVCDEMIVAQNIEFYSTCEHHLLPFLG
NISLGYIPKEKIVGISAIAKLIEIYSRRLQIQERLTTQIAETFDEIIEPRGVIVVCEAKHLCMSMQGVQKQNAIIKTSVL
KGLFKKDPKTRAEFMQLLKS
>Mature_180_residues
MENFFNQFFENIGEDKNREGLKETPKRVQELWKFLYKGYKEDPKVALKSAYFQGVCDEMIVAQNIEFYSTCEHHLLPFLG
NISLGYIPKEKIVGISAIAKLIEIYSRRLQIQERLTTQIAETFDEIIEPRGVIVVCEAKHLCMSMQGVQKQNAIIKTSVL
KGLFKKDPKTRAEFMQLLKS

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family [H]

Homologues:

Organism=Homo sapiens, GI66932968, Length=176, Percent_Identity=46.0227272727273, Blast_Score=184, Evalue=3e-47,
Organism=Homo sapiens, GI4503949, Length=176, Percent_Identity=46.0227272727273, Blast_Score=184, Evalue=3e-47,
Organism=Homo sapiens, GI66932970, Length=134, Percent_Identity=44.0298507462687, Blast_Score=134, Evalue=4e-32,
Organism=Homo sapiens, GI66932972, Length=134, Percent_Identity=44.0298507462687, Blast_Score=134, Evalue=6e-32,
Organism=Escherichia coli, GI1788476, Length=168, Percent_Identity=32.1428571428571, Blast_Score=105, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17560486, Length=178, Percent_Identity=46.6292134831461, Blast_Score=188, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6321706, Length=168, Percent_Identity=47.6190476190476, Blast_Score=167, Evalue=7e-43,
Organism=Drosophila melanogaster, GI24656782, Length=180, Percent_Identity=45.5555555555556, Blast_Score=169, Evalue=8e-43,
Organism=Drosophila melanogaster, GI24656796, Length=180, Percent_Identity=45.5555555555556, Blast_Score=169, Evalue=1e-42,
Organism=Drosophila melanogaster, GI24656791, Length=180, Percent_Identity=45.5555555555556, Blast_Score=169, Evalue=1e-42,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =3.5.4.16 [H]

Molecular weight: Translated: 20842; Mature: 20842

Theoretical pI: Translated: 8.60; Mature: 8.60

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1 ; PS00860 GTP_CYCLOHYDROL_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENFFNQFFENIGEDKNREGLKETPKRVQELWKFLYKGYKEDPKVALKSAYFQGVCDEMI
CCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
VAQNIEFYSTCEHHLLPFLGNISLGYIPKEKIVGISAIAKLIEIYSRRLQIQERLTTQIA
HHHCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ETFDEIIEPRGVIVVCEAKHLCMSMQGVQKQNAIIKTSVLKGLFKKDPKTRAEFMQLLKS
HHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHC
>Mature Secondary Structure
MENFFNQFFENIGEDKNREGLKETPKRVQELWKFLYKGYKEDPKVALKSAYFQGVCDEMI
CCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
VAQNIEFYSTCEHHLLPFLGNISLGYIPKEKIVGISAIAKLIEIYSRRLQIQERLTTQIA
HHHCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ETFDEIIEPRGVIVVCEAKHLCMSMQGVQKQNAIIKTSVLKGLFKKDPKTRAEFMQLLKS
HHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA