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The map label for this gene is murD

Identifier: 188527667

GI number: 188527667

Start: 869042

End: 870310

Strand: Reverse

Name: murD

Synonym: HPSH_04445

Alternate gene names: 188527667

Gene position: 870310-869042 (Counterclockwise)

Preceding gene: 188527668

Following gene: 188527664

Centisome position: 54.11

GC content: 37.35

Gene sequence:

>1269_bases
ATGAAAATCTCTTTATTGGGGCATGGCAAAACCACTCTAGCCCTAGGGCGTTTTTTTAGAAAAAACCATAACGAAGTCAA
ATTTTTTGACGACCAATTCACTTCATCTTTTAAAGATAGAGAGGGTTTTGTTTGCTATCCCAGTAAGGATTTCAACCCTA
ATGATTCCCAACTAGAGATAGTCAGCCCTGGCATTAGTTTCACGCACCCTTTAGTGATAAAAGCCAAGCATTTAGTGAGC
GAATACGATTATATTGATAGCTTGTTTGATTTGGTTTTCACGCCTACTATAATAAGTATTAGCGGCACTAATGGTAAAAC
CACCACGACAGAAATGCTCACCATGCTTTTAGAAGATTTTAAGGCTGTGAGTGGGGGGAATATCGGCACGCCCTTGATTG
AATTGTTTGAAAAGCAATCGCCCTTGTGGGTGTTAGAAACAAGCTCCTTTTCTTTGCATTACACCAATAAGGCTTACCCT
TTAATCTACCTGCTCATCAATGTGGAAGCCGATCATTTGACCTGGCATTGCAATTTTGAAAATTATTTGAACGCTAAACT
CAAGGTTTTAACATTGATGCCTAAAACTTCGCTCGCTATCCTCCCTTTAAAATTCAAAGAACATTCAAGCGTTCAAAATT
CGCAAGTGCAAAAAATCTTTTTTGACAAAAGCGAAGAGGTTTTAGAGCGTTTAAAAATCCCTTCTAACGCCCTTTTGTTT
AAGGGAGCGTTTTTATTAGACGCTGCCTTAGCCCTTTTAGTTTATGAGCAATTTTTAAAAATAAAGAATTTAAAATGGCA
AGATTATAGAGAAAACGCCCTTAAAAAACTGAACGCTTTTAAAGTCGGCTCGCATAAAATGGAAGAATTTAGGGATAAAC
AAGGGCGTTTGTGGGTAGATGACAGCAAAGCCACGAACATTGATGCTACCTTGCAAGCCCTAAAAACCTTTAAAAACCAA
AAAATCCATTTGATTTTAGGGGGCGATATTAAAGGGGTCAATTTAACCCCCCTTTTTGAAGAATTTAAAAATTATGAAAT
AAGCCTTTATGCGATAGGATCAAGCGCTTCTATCATCCAAGCCTTAGCGTTAGAATTTAATGTTTCTTGTCAGGTTTGTT
TGGAGTTAGAAAAAGCGGTTCAAGAAATTAAAAGCGTTTTATCGCAAAATGAAATCGCTTTGCTTTCCCCAAGCGCGGCC
AGTTTGGATCAATTTTCTTCGTATAAAGAAAGGGGTGAAAGATTTAAGGCGTTTGTTTTAAAAGATTAA

Upstream 100 bases:

>100_bases
GAGCAAAAGGGTTGGGCAGAAAACAAGGTGATCGTGCGTTTTTGGATCATTTCTATGCTGAGTAATTTAGTCGCTCTTTT
AAGCTTGAAGGTGCGTTAAA

Downstream 100 bases:

>100_bases
AGCGCATGCACCACTTTGTCTAATTGTGAAATTTTTTGAAAAATCTCGTTCCGTTCTGCTTCGTTTGAAACTTCCATAGA
AACATTAAAGCTATAAAATT

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MKISLLGHGKTTLALGRFFRKNHNEVKFFDDQFTSSFKDREGFVCYPSKDFNPNDSQLEIVSPGISFTHPLVIKAKHLVS
EYDYIDSLFDLVFTPTIISISGTNGKTTTTEMLTMLLEDFKAVSGGNIGTPLIELFEKQSPLWVLETSSFSLHYTNKAYP
LIYLLINVEADHLTWHCNFENYLNAKLKVLTLMPKTSLAILPLKFKEHSSVQNSQVQKIFFDKSEEVLERLKIPSNALLF
KGAFLLDAALALLVYEQFLKIKNLKWQDYRENALKKLNAFKVGSHKMEEFRDKQGRLWVDDSKATNIDATLQALKTFKNQ
KIHLILGGDIKGVNLTPLFEEFKNYEISLYAIGSSASIIQALALEFNVSCQVCLELEKAVQEIKSVLSQNEIALLSPSAA
SLDQFSSYKERGERFKAFVLKD

Sequences:

>Translated_422_residues
MKISLLGHGKTTLALGRFFRKNHNEVKFFDDQFTSSFKDREGFVCYPSKDFNPNDSQLEIVSPGISFTHPLVIKAKHLVS
EYDYIDSLFDLVFTPTIISISGTNGKTTTTEMLTMLLEDFKAVSGGNIGTPLIELFEKQSPLWVLETSSFSLHYTNKAYP
LIYLLINVEADHLTWHCNFENYLNAKLKVLTLMPKTSLAILPLKFKEHSSVQNSQVQKIFFDKSEEVLERLKIPSNALLF
KGAFLLDAALALLVYEQFLKIKNLKWQDYRENALKKLNAFKVGSHKMEEFRDKQGRLWVDDSKATNIDATLQALKTFKNQ
KIHLILGGDIKGVNLTPLFEEFKNYEISLYAIGSSASIIQALALEFNVSCQVCLELEKAVQEIKSVLSQNEIALLSPSAA
SLDQFSSYKERGERFKAFVLKD
>Mature_422_residues
MKISLLGHGKTTLALGRFFRKNHNEVKFFDDQFTSSFKDREGFVCYPSKDFNPNDSQLEIVSPGISFTHPLVIKAKHLVS
EYDYIDSLFDLVFTPTIISISGTNGKTTTTEMLTMLLEDFKAVSGGNIGTPLIELFEKQSPLWVLETSSFSLHYTNKAYP
LIYLLINVEADHLTWHCNFENYLNAKLKVLTLMPKTSLAILPLKFKEHSSVQNSQVQKIFFDKSEEVLERLKIPSNALLF
KGAFLLDAALALLVYEQFLKIKNLKWQDYRENALKKLNAFKVGSHKMEEFRDKQGRLWVDDSKATNIDATLQALKTFKNQ
KIHLILGGDIKGVNLTPLFEEFKNYEISLYAIGSSASIIQALALEFNVSCQVCLELEKAVQEIKSVLSQNEIALLSPSAA
SLDQFSSYKERGERFKAFVLKD

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=391, Percent_Identity=26.8542199488491, Blast_Score=130, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR005762 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 47899; Mature: 47899

Theoretical pI: Translated: 7.65; Mature: 7.65

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKISLLGHGKTTLALGRFFRKNHNEVKFFDDQFTSSFKDREGFVCYPSKDFNPNDSQLEI
CEEEEECCCCHHHHHHHHHHCCCCCEEEECHHHHHCCCCCCCEEEECCCCCCCCCCEEEE
VSPGISFTHPLVIKAKHLVSEYDYIDSLFDLVFTPTIISISGTNGKTTTTEMLTMLLEDF
ECCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHH
KAVSGGNIGTPLIELFEKQSPLWVLETSSFSLHYTNKAYPLIYLLINVEADHLTWHCNFE
HHCCCCCCCCHHHHHHHCCCCEEEEECCCEEEEECCCCCEEEEEEEEEEECEEEEEECHH
NYLNAKLKVLTLMPKTSLAILPLKFKEHSSVQNSQVQKIFFDKSEEVLERLKIPSNALLF
HHCCCEEEEEEECCCCCEEEEEEEECCCCCCCHHHHHHHHCCCHHHHHHHHCCCCCCEEE
KGAFLLDAALALLVYEQFLKIKNLKWQDYRENALKKLNAFKVGSHKMEEFRDKQGRLWVD
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCEEEE
DSKATNIDATLQALKTFKNQKIHLILGGDIKGVNLTPLFEEFKNYEISLYAIGSSASIIQ
CCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHCCCEEEEEEECCCHHHHH
ALALEFNVSCQVCLELEKAVQEIKSVLSQNEIALLSPSAASLDQFSSYKERGERFKAFVL
HHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHCCCEEEEEE
KD
CC
>Mature Secondary Structure
MKISLLGHGKTTLALGRFFRKNHNEVKFFDDQFTSSFKDREGFVCYPSKDFNPNDSQLEI
CEEEEECCCCHHHHHHHHHHCCCCCEEEECHHHHHCCCCCCCEEEECCCCCCCCCCEEEE
VSPGISFTHPLVIKAKHLVSEYDYIDSLFDLVFTPTIISISGTNGKTTTTEMLTMLLEDF
ECCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHH
KAVSGGNIGTPLIELFEKQSPLWVLETSSFSLHYTNKAYPLIYLLINVEADHLTWHCNFE
HHCCCCCCCCHHHHHHHCCCCEEEEECCCEEEEECCCCCEEEEEEEEEEECEEEEEECHH
NYLNAKLKVLTLMPKTSLAILPLKFKEHSSVQNSQVQKIFFDKSEEVLERLKIPSNALLF
HHCCCEEEEEEECCCCCEEEEEEEECCCCCCCHHHHHHHHCCCHHHHHHHHCCCCCCEEE
KGAFLLDAALALLVYEQFLKIKNLKWQDYRENALKKLNAFKVGSHKMEEFRDKQGRLWVD
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCEEEE
DSKATNIDATLQALKTFKNQKIHLILGGDIKGVNLTPLFEEFKNYEISLYAIGSSASIIQ
CCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHCCCEEEEEEECCCHHHHH
ALALEFNVSCQVCLELEKAVQEIKSVLSQNEIALLSPSAASLDQFSSYKERGERFKAFVL
HHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHCCCEEEEEE
KD
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA