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The map label for this gene is bioF [C]

Identifier: 188527553

GI number: 188527553

Start: 757738

End: 758859

Strand: Direct

Name: bioF [C]

Synonym: HPSH_03875

Alternate gene names: 188527553

Gene position: 757738-758859 (Clockwise)

Preceding gene: 188527552

Following gene: 188527554

Centisome position: 47.11

GC content: 38.41

Gene sequence:

>1122_bases
ATGTTTTCTAAATCCTTAGAAGCCCTACACCATGCCAAACGATACCGCAAAAGAGAATTGTTTGACCCTTTATTAAAGGA
TTACGCTTCTAATGATTATTTGGGTTTGAGCGTTAAAAAAGATTTGCTTCAAAACGCTTTTAACAAGCTCCAATCCTATA
ACGCTCATTCCCCCAAGGCTTCCATGCTAGTGAATGGTTACCACCCTTTGCATGCAGAATTAGAAGAACGATTAGCGGAT
TTGTTGGGGTTTGAAAGCGCTCTTTTAGTGGGGAGTGGTTTTTTGGGTAATCTGGCTTTAATAGACACCCTTTTAGTCAA
AAACGCCCTCTTATTCATGGACGAACACTACCATGCAAGCGGGATTTTTAGCACCAAAGCCAAGCCTAATCAAGTGGTTT
TTTTCTCGCACAATGACGCTAAAGATTTGCAACAAAAACTCTTTAACGCCCCTAAAAATAAACTCAAATTCATAGCCATT
GAAGGGGTTTATTCTATGGATGCGAGCGTCGCTCCTTATGATTTTTATGCAATCACTCAAGAGATTCCTAACGCTTTTTT
AATCGTTGATGAAGCCCATAGTTTTGGGACTATCGGCGAGAATTTGTTGGGTTTTTTAGAATATTATCGCATCAAAGGAA
AAGACAAAATCATTAAGCTCAGCACTTTCTCTAAAGCGCTTGCGAGCTATGGGGCGTGTATTTTAGCCCCTTTACAAATC
ATAGAGTTTTTAACCAATCGCGCTAAAAGCGTGATTTACACCACCGCTTTAAGCCTGTTAGACACCGCTTTAACTTTGGC
TCATTTAGAATACTTTATCGTGCAAAAACAAGAATTAAAAAATGAGCTTAGCAAACACCAACAGATTATTTTTGAAACCT
TAGGTATTAGAACGCCCACAGGATTTTTCACTTTAGAATTTGAAAACAATCTTGCTCTTTTAAACGCTCATCGTTTTTTG
AAAGAAAAGGGGTTTTTAGTGGGAGCTATCCGCCCCCCCACGGTTTCTAAACCCCTTTTACGCATCTCTTTATCCCTCAA
AAACAGCTTACAAGACACTAAAGAGCTTGCGAACACCCTTTTAAATTATTCTAAAATACAATCTTCTTTTAAGAGTGATT
AA

Upstream 100 bases:

>100_bases
GAAAATATTGATAAGATGTTTTACGAAAAACAAGACGAACTCAATAAAATCATTGAAAAAATTCAAAAAGGCGAATGATT
CCATTCAATGAAGTGTTTTT

Downstream 100 bases:

>100_bases
TGCTAAAAAAAATTTTTTATGGTTTTATCGTTTTATTTTTGATTATCGTAGGGTTGTTAGCCATTCTTATCGCTCAAGTT
TGGGTAACTACAGATAAGGA

Product: 8-amino-7-oxononanoate synthase (bioF)

Products: NA

Alternate protein names: AONS/AKB ligase; 7-keto-8-amino-pelargonic acid synthase; 7-KAP synthase; KAPA synthase; 8-amino-7-ketopelargonate synthase; Alpha-oxoamine synthase; L-alanine--pimeloyl-CoA ligase [H]

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MFSKSLEALHHAKRYRKRELFDPLLKDYASNDYLGLSVKKDLLQNAFNKLQSYNAHSPKASMLVNGYHPLHAELEERLAD
LLGFESALLVGSGFLGNLALIDTLLVKNALLFMDEHYHASGIFSTKAKPNQVVFFSHNDAKDLQQKLFNAPKNKLKFIAI
EGVYSMDASVAPYDFYAITQEIPNAFLIVDEAHSFGTIGENLLGFLEYYRIKGKDKIIKLSTFSKALASYGACILAPLQI
IEFLTNRAKSVIYTTALSLLDTALTLAHLEYFIVQKQELKNELSKHQQIIFETLGIRTPTGFFTLEFENNLALLNAHRFL
KEKGFLVGAIRPPTVSKPLLRISLSLKNSLQDTKELANTLLNYSKIQSSFKSD

Sequences:

>Translated_373_residues
MFSKSLEALHHAKRYRKRELFDPLLKDYASNDYLGLSVKKDLLQNAFNKLQSYNAHSPKASMLVNGYHPLHAELEERLAD
LLGFESALLVGSGFLGNLALIDTLLVKNALLFMDEHYHASGIFSTKAKPNQVVFFSHNDAKDLQQKLFNAPKNKLKFIAI
EGVYSMDASVAPYDFYAITQEIPNAFLIVDEAHSFGTIGENLLGFLEYYRIKGKDKIIKLSTFSKALASYGACILAPLQI
IEFLTNRAKSVIYTTALSLLDTALTLAHLEYFIVQKQELKNELSKHQQIIFETLGIRTPTGFFTLEFENNLALLNAHRFL
KEKGFLVGAIRPPTVSKPLLRISLSLKNSLQDTKELANTLLNYSKIQSSFKSD
>Mature_373_residues
MFSKSLEALHHAKRYRKRELFDPLLKDYASNDYLGLSVKKDLLQNAFNKLQSYNAHSPKASMLVNGYHPLHAELEERLAD
LLGFESALLVGSGFLGNLALIDTLLVKNALLFMDEHYHASGIFSTKAKPNQVVFFSHNDAKDLQQKLFNAPKNKLKFIAI
EGVYSMDASVAPYDFYAITQEIPNAFLIVDEAHSFGTIGENLLGFLEYYRIKGKDKIIKLSTFSKALASYGACILAPLQI
IEFLTNRAKSVIYTTALSLLDTALTLAHLEYFIVQKQELKNELSKHQQIIFETLGIRTPTGFFTLEFENNLALLNAHRFL
KEKGFLVGAIRPPTVSKPLLRISLSLKNSLQDTKELANTLLNYSKIQSSFKSD

Specific function: Catalyzes the decarboxylative condensation of pimeloyl- CoA and L-alanine to produce 8-amino-7-oxononanoate (AON), coenzyme A and/or converts 2-amino-3-ketobutyrate to glycine and acetyl-CoA [H]

COG id: COG0156

COG function: function code H; 7-keto-8-aminopelargonate synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI4758668, Length=240, Percent_Identity=30, Blast_Score=108, Evalue=8e-24,
Organism=Homo sapiens, GI83977442, Length=350, Percent_Identity=29.1428571428571, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI83977440, Length=350, Percent_Identity=29.1428571428571, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI83977444, Length=350, Percent_Identity=29.1428571428571, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI7657118, Length=353, Percent_Identity=25.7790368271955, Blast_Score=101, Evalue=9e-22,
Organism=Homo sapiens, GI284448556, Length=353, Percent_Identity=25.7790368271955, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI119220554, Length=254, Percent_Identity=25.5905511811024, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI4502025, Length=354, Percent_Identity=25.7062146892655, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI40316939, Length=354, Percent_Identity=25.7062146892655, Blast_Score=94, Evalue=3e-19,
Organism=Escherichia coli, GI1786993, Length=345, Percent_Identity=29.8550724637681, Blast_Score=128, Evalue=8e-31,
Organism=Escherichia coli, GI1790046, Length=336, Percent_Identity=27.6785714285714, Blast_Score=105, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI32566772, Length=240, Percent_Identity=28.3333333333333, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI71994529, Length=238, Percent_Identity=28.1512605042017, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17560912, Length=197, Percent_Identity=29.4416243654822, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17560914, Length=197, Percent_Identity=29.4416243654822, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI71982617, Length=176, Percent_Identity=27.2727272727273, Blast_Score=68, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI71982625, Length=180, Percent_Identity=27.2222222222222, Blast_Score=68, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6320438, Length=378, Percent_Identity=26.984126984127, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6320267, Length=254, Percent_Identity=27.1653543307087, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24662918, Length=293, Percent_Identity=29.3515358361775, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI17136286, Length=196, Percent_Identity=29.5918367346939, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI17137420, Length=252, Percent_Identity=29.7619047619048, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24653280, Length=262, Percent_Identity=25.1908396946565, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24653276, Length=262, Percent_Identity=25.1908396946565, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24653278, Length=262, Percent_Identity=25.1908396946565, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001917
- InterPro:   IPR004839
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: =2.3.1.29; =2.3.1.47 [H]

Molecular weight: Translated: 41990; Mature: 41990

Theoretical pI: Translated: 9.34; Mature: 9.34

Prosite motif: PS00599 AA_TRANSFER_CLASS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSKSLEALHHAKRYRKRELFDPLLKDYASNDYLGLSVKKDLLQNAFNKLQSYNAHSPKA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCH
SMLVNGYHPLHAELEERLADLLGFESALLVGSGFLGNLALIDTLLVKNALLFMDEHYHAS
HEEECCCCCHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
GIFSTKAKPNQVVFFSHNDAKDLQQKLFNAPKNKLKFIAIEGVYSMDASVAPYDFYAITQ
CEEECCCCCCEEEEEECCCHHHHHHHHHHCCCHHEEEEEEECEEECCCCCCCHHHHHHHH
EIPNAFLIVDEAHSFGTIGENLLGFLEYYRIKGKDKIIKLSTFSKALASYGACILAPLQI
HCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHCCHHHHHHHHH
IEFLTNRAKSVIYTTALSLLDTALTLAHLEYFIVQKQELKNELSKHQQIIFETLGIRTPT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GFFTLEFENNLALLNAHRFLKEKGFLVGAIRPPTVSKPLLRISLSLKNSLQDTKELANTL
CEEEEEECCCEEEHHHHHHHHHCCCEEEECCCCCCCCCHHHEEHHHHHHHHHHHHHHHHH
LNYSKIQSSFKSD
HHHHHHHHHHCCC
>Mature Secondary Structure
MFSKSLEALHHAKRYRKRELFDPLLKDYASNDYLGLSVKKDLLQNAFNKLQSYNAHSPKA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCH
SMLVNGYHPLHAELEERLADLLGFESALLVGSGFLGNLALIDTLLVKNALLFMDEHYHAS
HEEECCCCCHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
GIFSTKAKPNQVVFFSHNDAKDLQQKLFNAPKNKLKFIAIEGVYSMDASVAPYDFYAITQ
CEEECCCCCCEEEEEECCCHHHHHHHHHHCCCHHEEEEEEECEEECCCCCCCHHHHHHHH
EIPNAFLIVDEAHSFGTIGENLLGFLEYYRIKGKDKIIKLSTFSKALASYGACILAPLQI
HCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHCCHHHHHHHHH
IEFLTNRAKSVIYTTALSLLDTALTLAHLEYFIVQKQELKNELSKHQQIIFETLGIRTPT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GFFTLEFENNLALLNAHRFLKEKGFLVGAIRPPTVSKPLLRISLSLKNSLQDTKELANTL
CEEEEEECCCEEEHHHHHHHHHCCCEEEECCCCCCCCCHHHEEHHHHHHHHHHHHHHHHH
LNYSKIQSSFKSD
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]