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The map label for this gene is ogt [H]

Identifier: 188527477

GI number: 188527477

Start: 666524

End: 667030

Strand: Direct

Name: ogt [H]

Synonym: HPSH_03485

Alternate gene names: 188527477

Gene position: 666524-667030 (Clockwise)

Preceding gene: 188527476

Following gene: 188527498

Centisome position: 41.44

GC content: 37.28

Gene sequence:

>507_bases
ATGTTTTTATACCACTACTATTTTAAGACCCCTAAGAGTTTCCCTTTAGAGTATTTGCATTTGTGCGCTAATGAGAGCCA
TTTATTAAGATTGGATTTTGATGCGACCAATTTTTCTCATAACACCCCTATGAATACCCCATTAAAACTCAGCGTTCAAG
CCTTGGAGCGTTATTTCTTGGGGCAACTTTTTGAATTTAACGCGCCTTTAGATTTGATAGGGACTTTTTTTCAAAAACAA
GTTTGGTCTGCGTTAATGACTATCCCTTATGGCAAGACAAAAAGTTACGATGAAATCGCAAAGCTCATTAATAACCCTAG
ATCTTGCAGGGCCATTGGCAACGCTAATCGCAATAACCCTATTTCTTTGATCGTGCCTTGTCATAGAGTGGTGCGTAAAA
ATGGCGCTTTAGGGGGGTATAATGGGGGCATAGAAGTCAAAAAGTGGCTGTTAGAATTTGAAAGTAAAATTTTAAACCAG
CAAGCTAAAAACTTTTTAATTTCTTAA

Upstream 100 bases:

>100_bases
GATCATGGGGCTTTTGAATGAAAAAATGCATAAAGCTTTGATTTTGGGGGTGTATGGTTTGTCGCTATTGATTGTTTTAT
ACAAACTCTTTTTTTAATTG

Downstream 100 bases:

>100_bases
AAAATGGGGCTTTGATTATTTTATGGTAAAAATCATTTTTTTAAGGGAGTAGGGGGGTGGTTTTAATTTTCTTCCCAAAT
GCTCGCCGCTTCTTCTAAAC

Product: methylated-DNA--protein-cysteine methyltransferase (dat1)

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 168; Mature: 168

Protein sequence:

>168_residues
MFLYHYYFKTPKSFPLEYLHLCANESHLLRLDFDATNFSHNTPMNTPLKLSVQALERYFLGQLFEFNAPLDLIGTFFQKQ
VWSALMTIPYGKTKSYDEIAKLINNPRSCRAIGNANRNNPISLIVPCHRVVRKNGALGGYNGGIEVKKWLLEFESKILNQ
QAKNFLIS

Sequences:

>Translated_168_residues
MFLYHYYFKTPKSFPLEYLHLCANESHLLRLDFDATNFSHNTPMNTPLKLSVQALERYFLGQLFEFNAPLDLIGTFFQKQ
VWSALMTIPYGKTKSYDEIAKLINNPRSCRAIGNANRNNPISLIVPCHRVVRKNGALGGYNGGIEVKKWLLEFESKILNQ
QAKNFLIS
>Mature_168_residues
MFLYHYYFKTPKSFPLEYLHLCANESHLLRLDFDATNFSHNTPMNTPLKLSVQALERYFLGQLFEFNAPLDLIGTFFQKQ
VWSALMTIPYGKTKSYDEIAKLINNPRSCRAIGNANRNNPISLIVPCHRVVRKNGALGGYNGGIEVKKWLLEFESKILNQ
QAKNFLIS

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=86, Percent_Identity=41.8604651162791, Blast_Score=90, Evalue=1e-18,
Organism=Escherichia coli, GI1787596, Length=133, Percent_Identity=39.8496240601504, Blast_Score=103, Evalue=6e-24,
Organism=Escherichia coli, GI1788542, Length=134, Percent_Identity=41.044776119403, Blast_Score=102, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI115533068, Length=84, Percent_Identity=46.4285714285714, Blast_Score=80, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI115533070, Length=84, Percent_Identity=46.4285714285714, Blast_Score=80, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6320001, Length=103, Percent_Identity=37.8640776699029, Blast_Score=78, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 19349; Mature: 19349

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLYHYYFKTPKSFPLEYLHLCANESHLLRLDFDATNFSHNTPMNTPLKLSVQALERYFL
CEEEEEEECCCCCCCHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHH
GQLFEFNAPLDLIGTFFQKQVWSALMTIPYGKTKSYDEIAKLINNPRSCRAIGNANRNNP
HHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHCCCCCCCC
ISLIVPCHRVVRKNGALGGYNGGIEVKKWLLEFESKILNQQAKNFLIS
EEEEECHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFLYHYYFKTPKSFPLEYLHLCANESHLLRLDFDATNFSHNTPMNTPLKLSVQALERYFL
CEEEEEEECCCCCCCHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHH
GQLFEFNAPLDLIGTFFQKQVWSALMTIPYGKTKSYDEIAKLINNPRSCRAIGNANRNNP
HHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHCCCCCCCC
ISLIVPCHRVVRKNGALGGYNGGIEVKKWLLEFESKILNQQAKNFLIS
EEEEECHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]