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The map label for this gene is ansA [H]

Identifier: 188527432

GI number: 188527432

Start: 616445

End: 617437

Strand: Direct

Name: ansA [H]

Synonym: HPSH_03250

Alternate gene names: 188527432

Gene position: 616445-617437 (Clockwise)

Preceding gene: 188527430

Following gene: 188527437

Centisome position: 38.32

GC content: 45.42

Gene sequence:

>993_bases
ATGGCTCAAAATTTACCCACGATTGCTTTATTGGCGATGGGAGGGACGATTGCAGGGAGTGGCGTTAATGCGAATTTGGG
TAGTTATAAAAGTGGCGAGTTGGGCATCAAAGAGCTTCTTAAAGCTATCCCTAGTCTTAACAAGATCGCTCGCATTCAAG
GGGAGCAGATTTCTAACATCGGCTCACAAGACATGGATGAAGAGGTGTGGTTCAAACTCGCCAAACGCGCCCAAGAATTG
CTAGATGGTAGCCGCATTCAAGGTGTGGTTATCACGCATGGCACGGACACTTTAGAAGAGAGCGCGTATTTTTTAAATTT
AGTTTTATGTTCCACAAAACCGGTTGTGCTAGTGGGAGCGATGCGTAATTTTGCTTCTTTGAGCGCGGATGGGGCTTTGA
ATTTGTATAACGCGCTGAGCGTAGCGGCCAATGAAAAAAGTGCTAATAAAGGCGTGTTGGTGGTGATGGACGATAATATT
TTTAGCGCCAGAGAAGTGGCTAAAACGCACACCACCCACACTTCCACCTTTAAAGCCCTAAATAGCGGCGCGATAGGGAG
CGTGTATTATGGTAAAACACGCTATTACATGCAACCTTTGAGAAAACACACCACAGAGAGCGAATTTTCCCTTTCACAAC
TCAAAACCCCCCTGCCTAAAGTGGATATTATTTACACGCATGCCGGCATGACCCTTGATTTATTCCAAGCGAGCCTAAAC
TCGCATGCAAAAGGCGTTGTGATAGCTGGGGTGGGTAATGGGAATGTGAGCGCTGGGTTTTTAAAAGCGATGCAAGAAGC
GAGCAAAATGGGGGTGGTTATTGTTCGTTCTAGCAGGGTAGGTAGCGGTGGAATTACTTCAGGAGAGATTGATGACAAGG
CCTTTATCACAAGCGACAATTTAAACCCCCAAAAAGCCAGGGTGCTTTTACAACTCGCTCTAACCAAAACGAATGATAAG
GCAAAAATCCAAGAAATGTTTGAAGAGTATTGA

Upstream 100 bases:

>100_bases
AAAAGGCATCCACCATTAACCCTTTTTAAATTTATTTTAACAACGATTCACTATACTACAAAAATCTTTAAATCCTTTTA
TTTTGTTTGAAGGGGAAGTT

Downstream 100 bases:

>100_bases
AACCCTCTTTAGAATGGTTTGAAACGCTGAAGCGGTTTTAGGTTTTTATTTTTTGATTGGAATTTAATCAAATCGTGGTT
GGGGTAGAAGTTGCCTTTAA

Product: L-asparaginase II (ansB)

Products: NA

Alternate protein names: L-ASNase; L-asparagine amidohydrolase [H]

Number of amino acids: Translated: 330; Mature: 329

Protein sequence:

>330_residues
MAQNLPTIALLAMGGTIAGSGVNANLGSYKSGELGIKELLKAIPSLNKIARIQGEQISNIGSQDMDEEVWFKLAKRAQEL
LDGSRIQGVVITHGTDTLEESAYFLNLVLCSTKPVVLVGAMRNFASLSADGALNLYNALSVAANEKSANKGVLVVMDDNI
FSAREVAKTHTTHTSTFKALNSGAIGSVYYGKTRYYMQPLRKHTTESEFSLSQLKTPLPKVDIIYTHAGMTLDLFQASLN
SHAKGVVIAGVGNGNVSAGFLKAMQEASKMGVVIVRSSRVGSGGITSGEIDDKAFITSDNLNPQKARVLLQLALTKTNDK
AKIQEMFEEY

Sequences:

>Translated_330_residues
MAQNLPTIALLAMGGTIAGSGVNANLGSYKSGELGIKELLKAIPSLNKIARIQGEQISNIGSQDMDEEVWFKLAKRAQEL
LDGSRIQGVVITHGTDTLEESAYFLNLVLCSTKPVVLVGAMRNFASLSADGALNLYNALSVAANEKSANKGVLVVMDDNI
FSAREVAKTHTTHTSTFKALNSGAIGSVYYGKTRYYMQPLRKHTTESEFSLSQLKTPLPKVDIIYTHAGMTLDLFQASLN
SHAKGVVIAGVGNGNVSAGFLKAMQEASKMGVVIVRSSRVGSGGITSGEIDDKAFITSDNLNPQKARVLLQLALTKTNDK
AKIQEMFEEY
>Mature_329_residues
AQNLPTIALLAMGGTIAGSGVNANLGSYKSGELGIKELLKAIPSLNKIARIQGEQISNIGSQDMDEEVWFKLAKRAQELL
DGSRIQGVVITHGTDTLEESAYFLNLVLCSTKPVVLVGAMRNFASLSADGALNLYNALSVAANEKSANKGVLVVMDDNIF
SAREVAKTHTTHTSTFKALNSGAIGSVYYGKTRYYMQPLRKHTTESEFSLSQLKTPLPKVDIIYTHAGMTLDLFQASLNS
HAKGVVIAGVGNGNVSAGFLKAMQEASKMGVVIVRSSRVGSGGITSGEIDDKAFITSDNLNPQKARVLLQLALTKTNDKA
KIQEMFEEY

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1789327, Length=329, Percent_Identity=49.2401215805471, Blast_Score=302, Evalue=2e-83,
Organism=Escherichia coli, GI1788065, Length=230, Percent_Identity=30, Blast_Score=85, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6323189, Length=329, Percent_Identity=36.1702127659575, Blast_Score=191, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6323187, Length=329, Percent_Identity=36.1702127659575, Blast_Score=191, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6323186, Length=329, Percent_Identity=36.1702127659575, Blast_Score=191, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6323184, Length=329, Percent_Identity=36.1702127659575, Blast_Score=191, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6320527, Length=334, Percent_Identity=32.3353293413174, Blast_Score=174, Evalue=2e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004550
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 35349; Mature: 35218

Theoretical pI: Translated: 9.15; Mature: 9.15

Prosite motif: PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQNLPTIALLAMGGTIAGSGVNANLGSYKSGELGIKELLKAIPSLNKIARIQGEQISNI
CCCCCCEEEEEEECCEEECCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHCCHHHHCC
GSQDMDEEVWFKLAKRAQELLDGSRIQGVVITHGTDTLEESAYFLNLVLCSTKPVVLVGA
CCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHEEEEEEEECCCCEEEEEC
MRNFASLSADGALNLYNALSVAANEKSANKGVLVVMDDNIFSAREVAKTHTTHTSTFKAL
HHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHCCCCHHHHHHH
NSGAIGSVYYGKTRYYMQPLRKHTTESEFSLSQLKTPLPKVDIIYTHAGMTLDLFQASLN
CCCCCCEEEECCHHHHHHHHHHCCCCCCCCHHHHCCCCCCEEEEEEECCCCHHHHHHHHC
SHAKGVVIAGVGNGNVSAGFLKAMQEASKMGVVIVRSSRVGSGGITSGEIDDKAFITSDN
CCCCEEEEEECCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCEEEECCC
LNPQKARVLLQLALTKTNDKAKIQEMFEEY
CCHHHHHHHHHHHHHCCCCHHHHHHHHHCC
>Mature Secondary Structure 
AQNLPTIALLAMGGTIAGSGVNANLGSYKSGELGIKELLKAIPSLNKIARIQGEQISNI
CCCCCEEEEEEECCEEECCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHCCHHHHCC
GSQDMDEEVWFKLAKRAQELLDGSRIQGVVITHGTDTLEESAYFLNLVLCSTKPVVLVGA
CCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHEEEEEEEECCCCEEEEEC
MRNFASLSADGALNLYNALSVAANEKSANKGVLVVMDDNIFSAREVAKTHTTHTSTFKAL
HHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHCCCCHHHHHHH
NSGAIGSVYYGKTRYYMQPLRKHTTESEFSLSQLKTPLPKVDIIYTHAGMTLDLFQASLN
CCCCCCEEEECCHHHHHHHHHHCCCCCCCCHHHHCCCCCCEEEEEEECCCCHHHHHHHHC
SHAKGVVIAGVGNGNVSAGFLKAMQEASKMGVVIVRSSRVGSGGITSGEIDDKAFITSDN
CCCCEEEEEECCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCEEEECCC
LNPQKARVLLQLALTKTNDKAKIQEMFEEY
CCHHHHHHHHHHHHHCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]