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The map label for this gene is ispDF

Identifier: 188527236

GI number: 188527236

Start: 420278

End: 421498

Strand: Reverse

Name: ispDF

Synonym: HPSH_02215

Alternate gene names: 188527236

Gene position: 421498-420278 (Counterclockwise)

Preceding gene: 188527237

Following gene: 188527235

Centisome position: 26.2

GC content: 41.36

Gene sequence:

>1221_bases
ATGTCTTTGATTAGAGTGAATGGGGAAGCTTTTAAACTCTCTTTAGAAAGTTTGGAAGAAGACCCTTTTGAAAATAAAGA
AACGCTAGAAACGCTTGTCAAACAAACGAGCGTTGTTTTATTGGCCGCTGGGGAATCTAGGCGATTTTCTCAAACAATCA
AAAAACAATGGTTGCGCTCTAACCATACCCCCTTATGGCTCAGCGTTTATGAAAGCTTTAAAGAAGCCCTAAACTTTAAA
GAAATCCTTCTAGTCGTAAGCGAATTGGATTATATTTATATCAAACGCCATCACCCTGAAATCAAGCTTGTAAGAGGTGG
GGCATCAAGGCAGGAATCCGTGCGTAACGCTTTAAAAATAATTGATAGCGCTTACACGCTCACTAGCGATGTGGCTAGGG
GTTTGGCCAATATTGAAGCGCTCAAAAGTTTATTTTTAACCCTCCAACAAACCAGCCATTATTGCATCGCCCCTTACTTG
CCTTGCTATGACACGGCGATCTATTATAACGAGGTTTTAGACAGAGAAGCGATCAAACTCATTCAAACCCCGCAATTAAG
CCACACCAAAACGCTCCAATCAGCCCTAAATCAAGGGGATTTTAAAGATGAAAGCAGCGCGATTTTACAAGCTTTCCCTG
ATTTGGTGAGCTATATTGAAGGCAGTAAGGATTTGCACAAGCTCACCACGAGCGACGATTTGAAGCATTTCGCGCTCTTT
TTCAACCCAGCAAAAGACACTTTTATAGGCATGGGCTTTGATACGCATGCGTTTATTAAAGATAAGCCTATGGTTTTAGG
GGGGGTTGTTTTGGATTGCGAGTTTGGGTTAAAGGCCCATAGCGATGGCGATGCTTTGTTGCATGCGGTTATTGATGCGA
TTTTAGGGGCGATTAAAGGGGGGGATATTGGCGAATGGTTCCCTGATAATGACCCACAATACAAAAACGCCTCTTCTAAA
GAGCTTTTAAAAATCGTGTTGGATTTTTCTCAAAGCATTGGGTTTGAATTGCTTGAAATGGGAGCGACCATCTTTAGCGA
AATCCCTAAAATCACTCCTTACAAACCGACGATTTTAGAAAATTTGAGCCAACTTTTGGGTTTAGAAAAATCTCAAATCA
GCTTGAAAGCCACTACAATGGAAAAAATGGGGTTCATTGGCAAACAAGAAGGGCTGTTAGTCCAAGCGCATGTGAGCATG
CGTTATAAACAAAAACTTTAA

Upstream 100 bases:

>100_bases
AGAAAAGTATAAAGGCAAACCCAAACGATTCTTAGTTTTAGACTTGAATCAAGGTTATAGGATCATTTTGGTGAAATGAT
AGAGGTGGGTTGTTGGTCGC

Downstream 100 bases:

>100_bases
ATGTGATAAAATGTGATAAAAGGAAGAATTGATGAAAATCTTAATCATTGAAGACGATTTAGCACTAGCTAGGAGTATTT
CGCACAATTTGCATGATTTA

Product: bifunctional 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase/2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase protein

Products: NA

Alternate protein names: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase; 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT; 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase; MECDP-synthase; MECPS [H]

Number of amino acids: Translated: 406; Mature: 405

Protein sequence:

>406_residues
MSLIRVNGEAFKLSLESLEEDPFENKETLETLVKQTSVVLLAAGESRRFSQTIKKQWLRSNHTPLWLSVYESFKEALNFK
EILLVVSELDYIYIKRHHPEIKLVRGGASRQESVRNALKIIDSAYTLTSDVARGLANIEALKSLFLTLQQTSHYCIAPYL
PCYDTAIYYNEVLDREAIKLIQTPQLSHTKTLQSALNQGDFKDESSAILQAFPDLVSYIEGSKDLHKLTTSDDLKHFALF
FNPAKDTFIGMGFDTHAFIKDKPMVLGGVVLDCEFGLKAHSDGDALLHAVIDAILGAIKGGDIGEWFPDNDPQYKNASSK
ELLKIVLDFSQSIGFELLEMGATIFSEIPKITPYKPTILENLSQLLGLEKSQISLKATTMEKMGFIGKQEGLLVQAHVSM
RYKQKL

Sequences:

>Translated_406_residues
MSLIRVNGEAFKLSLESLEEDPFENKETLETLVKQTSVVLLAAGESRRFSQTIKKQWLRSNHTPLWLSVYESFKEALNFK
EILLVVSELDYIYIKRHHPEIKLVRGGASRQESVRNALKIIDSAYTLTSDVARGLANIEALKSLFLTLQQTSHYCIAPYL
PCYDTAIYYNEVLDREAIKLIQTPQLSHTKTLQSALNQGDFKDESSAILQAFPDLVSYIEGSKDLHKLTTSDDLKHFALF
FNPAKDTFIGMGFDTHAFIKDKPMVLGGVVLDCEFGLKAHSDGDALLHAVIDAILGAIKGGDIGEWFPDNDPQYKNASSK
ELLKIVLDFSQSIGFELLEMGATIFSEIPKITPYKPTILENLSQLLGLEKSQISLKATTMEKMGFIGKQEGLLVQAHVSM
RYKQKL
>Mature_405_residues
SLIRVNGEAFKLSLESLEEDPFENKETLETLVKQTSVVLLAAGESRRFSQTIKKQWLRSNHTPLWLSVYESFKEALNFKE
ILLVVSELDYIYIKRHHPEIKLVRGGASRQESVRNALKIIDSAYTLTSDVARGLANIEALKSLFLTLQQTSHYCIAPYLP
CYDTAIYYNEVLDREAIKLIQTPQLSHTKTLQSALNQGDFKDESSAILQAFPDLVSYIEGSKDLHKLTTSDDLKHFALFF
NPAKDTFIGMGFDTHAFIKDKPMVLGGVVLDCEFGLKAHSDGDALLHAVIDAILGAIKGGDIGEWFPDNDPQYKNASSKE
LLKIVLDFSQSIGFELLEMGATIFSEIPKITPYKPTILENLSQLLGLEKSQISLKATTMEKMGFIGKQEGLLVQAHVSMR
YKQKL

Specific function: Bifunctional enzyme that catalyzes the formation of 4- diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl- D-erythritol 4-phosphate (MEP) (ispD), and converts 4- diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate into 2-C- methyl-D-erythri

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the ispF family [H]

Homologues:

Organism=Escherichia coli, GI1789103, Length=148, Percent_Identity=45.2702702702703, Blast_Score=122, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001228
- InterPro:   IPR018294
- InterPro:   IPR003526
- InterPro:   IPR020555 [H]

Pfam domain/function: PF01128 IspD; PF02542 YgbB [H]

EC number: =2.7.7.60; =4.6.1.12 [H]

Molecular weight: Translated: 45542; Mature: 45411

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS01295 ISPD ; PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLIRVNGEAFKLSLESLEEDPFENKETLETLVKQTSVVLLAAGESRRFSQTIKKQWLRS
CCEEEECCCEEEEEHHHHCCCCCCCHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHC
NHTPLWLSVYESFKEALNFKEILLVVSELDYIYIKRHHPEIKLVRGGASRQESVRNALKI
CCCCCHHHHHHHHHHHHCHHHHHHHHHHCCEEEEEECCCCEEEEECCCCHHHHHHHHHHH
IDSAYTLTSDVARGLANIEALKSLFLTLQQTSHYCIAPYLPCYDTAIYYNEVLDREAIKL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHH
IQTPQLSHTKTLQSALNQGDFKDESSAILQAFPDLVSYIEGSKDLHKLTTSDDLKHFALF
HCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCHHEEHEE
FNPAKDTFIGMGFDTHAFIKDKPMVLGGVVLDCEFGLKAHSDGDALLHAVIDAILGAIKG
ECCCCCCEEECCCCCCEEECCCCEEECCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCC
GDIGEWFPDNDPQYKNASSKELLKIVLDFSQSIGFELLEMGATIFSEIPKITPYKPTILE
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
NLSQLLGLEKSQISLKATTMEKMGFIGKQEGLLVQAHVSMRYKQKL
HHHHHHCCCHHHEEEEHHHHHHHCCCCCCCCEEEEEEHHHHHHHCC
>Mature Secondary Structure 
SLIRVNGEAFKLSLESLEEDPFENKETLETLVKQTSVVLLAAGESRRFSQTIKKQWLRS
CEEEECCCEEEEEHHHHCCCCCCCHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHC
NHTPLWLSVYESFKEALNFKEILLVVSELDYIYIKRHHPEIKLVRGGASRQESVRNALKI
CCCCCHHHHHHHHHHHHCHHHHHHHHHHCCEEEEEECCCCEEEEECCCCHHHHHHHHHHH
IDSAYTLTSDVARGLANIEALKSLFLTLQQTSHYCIAPYLPCYDTAIYYNEVLDREAIKL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHH
IQTPQLSHTKTLQSALNQGDFKDESSAILQAFPDLVSYIEGSKDLHKLTTSDDLKHFALF
HCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCHHEEHEE
FNPAKDTFIGMGFDTHAFIKDKPMVLGGVVLDCEFGLKAHSDGDALLHAVIDAILGAIKG
ECCCCCCEEECCCCCCEEECCCCEEECCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCC
GDIGEWFPDNDPQYKNASSKELLKIVLDFSQSIGFELLEMGATIFSEIPKITPYKPTILE
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
NLSQLLGLEKSQISLKATTMEKMGFIGKQEGLLVQAHVSMRYKQKL
HHHHHHCCCHHHEEEEHHHHHHHCCCCCCCCEEEEEEHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]