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The map label for this gene is ruvB

Identifier: 188527198

GI number: 188527198

Start: 385026

End: 386036

Strand: Reverse

Name: ruvB

Synonym: HPSH_02025

Alternate gene names: 188527198

Gene position: 386036-385026 (Counterclockwise)

Preceding gene: 188527199

Following gene: 188527197

Centisome position: 24.0

GC content: 42.04

Gene sequence:

>1011_bases
ATGAAAGAACGGATAGTCAATTTAGAAACTTTGGATTTTGAAACTTCTCAAGAAGCGAGTTTGCGCCCTAATCTTTGGGA
AGATTATATCGGTCAAGAAAAGATTAAAAGCAATTTGCAAGTTTCTATTTGCGCGGCTAAAAAACGCCAAGAAAGTTTGG
ATCACATGCTCTTTTTTGGCCCGCCCGGTTTGGGTAAAACTTCAATAAGCCATATCATCGCTAAAGAAATGGAAACCAAT
CTCAAAATCACCGCCGCTCCCATGATAGAAAAAAGCGGTGATTTGGCCGCCATTTTGACGAATTTGCAAGCTAAAGACAT
TCTTTTTATTGATGAAATCCACCGGCTTAGCCCAGCGATTGAAGAGGTTTTATACCCGGCTATGGAAGATTTCAGGCTGG
ATATTATCATAGGATCAGGTCCGGCGGCTCAAACCATTAAAATTGATTTGCCTCCTTTCACCCTCATCGGTGCTACCACT
AGAGCCGGAATGCTCTCTAACCCCTTAAGAGACAGATTTGGCATGAGCTTTAGAATGCAATTTTATAGCCCTAGCGAACT
GGCCCTTATCATCAAAAAAGCCGCCGCCAAACTCAACCAAGACATCAAAGAAGAAAGTGCTGATGAAATCGCTAAAAGGA
GTAGAGGCACGCCAAGGATTGCTTTAAGGCTTTTAAAAAGGGTGCGCGATTTTGCGTTAGTCAAAAATTCAAGCTTGATG
GATTTAAACATCACTTTGCATGCTTTGAATGAATTAGGCGTGAATGAATTAGGCTTTGATGAAGCGGATTTGGCGTATTT
GTCTTTGTTGGCTAACGCTCAAGGACGACCGGTGGGTTTGAACACGATTGCAGCATCTATGAGAGAAGATGAAGGCACGA
TTGAAGATGTGATTGAGCCTTTTTTACTCGCTAATGGTTATTTAGAGCGCACCGCTAAAGGCAGAATCGCCACGCCTAAA
ACCCATGCGCTTTTAAAAATCCCCACTTTAAACCCCCAAACTTTATTTTAA

Upstream 100 bases:

>100_bases
ATACCTTAAGGGGAAAGAATTGGTTCAAAACGCTATCAAGCAATACGCTGATGATGTGAAAAAGGGAAACTTTCCTAACG
AATTAGAAAGTTATCATTAA

Downstream 100 bases:

>100_bases
TCTTGTTTAGAAAGAAAATTACACTACAATAACGATAAAATTTTAAAGGGTGTAAAAATAGATTATTATGTTTGGCATGG
GCTTTTTTGAAATCCTTGTG

Product: Holliday junction DNA helicase RuvB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MKERIVNLETLDFETSQEASLRPNLWEDYIGQEKIKSNLQVSICAAKKRQESLDHMLFFGPPGLGKTSISHIIAKEMETN
LKITAAPMIEKSGDLAAILTNLQAKDILFIDEIHRLSPAIEEVLYPAMEDFRLDIIIGSGPAAQTIKIDLPPFTLIGATT
RAGMLSNPLRDRFGMSFRMQFYSPSELALIIKKAAAKLNQDIKEESADEIAKRSRGTPRIALRLLKRVRDFALVKNSSLM
DLNITLHALNELGVNELGFDEADLAYLSLLANAQGRPVGLNTIAASMREDEGTIEDVIEPFLLANGYLERTAKGRIATPK
THALLKIPTLNPQTLF

Sequences:

>Translated_336_residues
MKERIVNLETLDFETSQEASLRPNLWEDYIGQEKIKSNLQVSICAAKKRQESLDHMLFFGPPGLGKTSISHIIAKEMETN
LKITAAPMIEKSGDLAAILTNLQAKDILFIDEIHRLSPAIEEVLYPAMEDFRLDIIIGSGPAAQTIKIDLPPFTLIGATT
RAGMLSNPLRDRFGMSFRMQFYSPSELALIIKKAAAKLNQDIKEESADEIAKRSRGTPRIALRLLKRVRDFALVKNSSLM
DLNITLHALNELGVNELGFDEADLAYLSLLANAQGRPVGLNTIAASMREDEGTIEDVIEPFLLANGYLERTAKGRIATPK
THALLKIPTLNPQTLF
>Mature_336_residues
MKERIVNLETLDFETSQEASLRPNLWEDYIGQEKIKSNLQVSICAAKKRQESLDHMLFFGPPGLGKTSISHIIAKEMETN
LKITAAPMIEKSGDLAAILTNLQAKDILFIDEIHRLSPAIEEVLYPAMEDFRLDIIIGSGPAAQTIKIDLPPFTLIGATT
RAGMLSNPLRDRFGMSFRMQFYSPSELALIIKKAAAKLNQDIKEESADEIAKRSRGTPRIALRLLKRVRDFALVKNSSLM
DLNITLHALNELGVNELGFDEADLAYLSLLANAQGRPVGLNTIAASMREDEGTIEDVIEPFLLANGYLERTAKGRIATPK
THALLKIPTLNPQTLF

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG2255

COG function: function code L; Holliday junction resolvasome, helicase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvB family [H]

Homologues:

Organism=Escherichia coli, GI1788167, Length=319, Percent_Identity=52.3510971786834, Blast_Score=348, Evalue=2e-97,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR004605
- InterPro:   IPR008823
- InterPro:   IPR008824
- InterPro:   IPR011991
- ProDom:   PD005323 [H]

Pfam domain/function: PF05491 RuvB_C; PF05496 RuvB_N [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 37266; Mature: 37266

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKERIVNLETLDFETSQEASLRPNLWEDYIGQEKIKSNLQVSICAAKKRQESLDHMLFFG
CCCCEEEEEEECCCCCCCCCCCCCHHHHHCCHHHHHCCCEEEEEEHHHHHHHHCCEEEEC
PPGLGKTSISHIIAKEMETNLKITAAPMIEKSGDLAAILTNLQAKDILFIDEIHRLSPAI
CCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHH
EEVLYPAMEDFRLDIIIGSGPAAQTIKIDLPPFTLIGATTRAGMLSNPLRDRFGMSFRMQ
HHHHHHHHHCEEEEEEECCCCCCEEEEEECCCEEEEECCHHCCHHCCHHHHHHCCEEEEE
FYSPSELALIIKKAAAKLNQDIKEESADEIAKRSRGTPRIALRLLKRVRDFALVKNSSLM
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEE
DLNITLHALNELGVNELGFDEADLAYLSLLANAQGRPVGLNTIAASMREDEGTIEDVIEP
EEEEEEEEHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHH
FLLANGYLERTAKGRIATPKTHALLKIPTLNPQTLF
HHHHCCHHHHHCCCCCCCCCCCEEEEECCCCCCCCC
>Mature Secondary Structure
MKERIVNLETLDFETSQEASLRPNLWEDYIGQEKIKSNLQVSICAAKKRQESLDHMLFFG
CCCCEEEEEEECCCCCCCCCCCCCHHHHHCCHHHHHCCCEEEEEEHHHHHHHHCCEEEEC
PPGLGKTSISHIIAKEMETNLKITAAPMIEKSGDLAAILTNLQAKDILFIDEIHRLSPAI
CCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHH
EEVLYPAMEDFRLDIIIGSGPAAQTIKIDLPPFTLIGATTRAGMLSNPLRDRFGMSFRMQ
HHHHHHHHHCEEEEEEECCCCCCEEEEEECCCEEEEECCHHCCHHCCHHHHHHCCEEEEE
FYSPSELALIIKKAAAKLNQDIKEESADEIAKRSRGTPRIALRLLKRVRDFALVKNSSLM
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEE
DLNITLHALNELGVNELGFDEADLAYLSLLANAQGRPVGLNTIAASMREDEGTIEDVIEP
EEEEEEEEHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHH
FLLANGYLERTAKGRIATPKTHALLKIPTLNPQTLF
HHHHCCHHHHHCCCCCCCCCCCEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA