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The map label for this gene is kgtP [H]

Identifier: 188527164

GI number: 188527164

Start: 351634

End: 352920

Strand: Reverse

Name: kgtP [H]

Synonym: HPSH_01855

Alternate gene names: 188527164

Gene position: 352920-351634 (Counterclockwise)

Preceding gene: 188527165

Following gene: 188527163

Centisome position: 21.94

GC content: 40.48

Gene sequence:

>1287_bases
ATGAACCCCCAAGCCCAACCAGCCACCAAAAAACCCTTAAGATCTCTTTTAGCCGCTAGTTCAGGTAATTTAGTAGAATG
GTATGACTTTTACGCTTATGCGTTCTTAGCGCCTTATTTTGCTAAGGAATTTACCCACACTAACGACCCCACTTTAGCGC
TAATCTCAGCTTTTTTAGTTTTCATGCTAGGGTTTTTCATGCGCCCTTTGGGGAGTTTGTTTTTTGGTAAATTGGGGGAT
AAAAAGGGGCGTAAAACTTCTATGGTGTATTCCATTATCCTTATGGCGCTAGGCTCTTTCATGCTCGCATTGCTCCCCAC
TAAAGAAATCGTAGGGGAATGGGCGTTCTTGTTTTTATTATTAGCCAGGCTTTTACAAGGCTTTAGCGTGGGAGGAGAAT
ACGGCGTGGTCGCTACTTATCTCTCTGAATTAGGCAAGAACGGCAAAAAAGGTTTTTATGGTTCTTTTCAATATGTAACT
TTAGTGGGAGGGCAACTCTTAGCTATTTTTTCACTCTTTATCGTTGAAAATATTTACACGCATGAGCAAATCAGCGCGTT
TGCTTGGCGTTATTTATTCGCTTTAGGGGGTATATTAGCCCTACTCTCGCTCTTTTTAAGAAATATCATGGAAGAAACTA
TGGATAGTCAAACAACTTCCAAAACCACCATTAAAGAAGAAACCCAAAGAGGCAGTTTGAAGGAGTTACTCAACCATAAA
AAAGCCTTAATGATAGTCTTTGGGCTAACTATGGGAGGGAGTTTGTGTTTTTATACTTTTACGGTGTATTTAAAAATCTT
TTTAACCAACAGCTCATCATTCAGCCCTAAAGAAAGCAGTTTTATCATGCTTTTAGCGCTCTCTTATTTTATCTTCTTAC
AACCCTTATGCGGGATGCTGGCTGATAAAATCAAACGCACCCAAATGCTGATGGTTTTTGCTATCGCAGGGCTTATTGTA
ACGCCCGTTGTCTTTTATGGTATCAAGCATGCTAGTGGCGTGTATGAAGCCCTATTTTATGAAATGCTTGCATTGAGTGC
GATGAGTTTTTACACTTGCATTGCTGGGGTCGTTAAAGCGGAATTATTCCCTGAACATGTGCGAGCGCTTGGCGTGGGTT
TAGCCTATGCGATTGCGAATGCGCTTTTTGGAGGGAGCGCGAGTTATGTAGCGTTGCAATTCAAACAACATGGTTTTGAA
TGGGGGTTTGTGGGCTATGTCATGTTTAGTATTGTTATCTTTATGGTTATGGTTATCATATTCCCTAAAAAAACCTATTT
GGAATAA

Upstream 100 bases:

>100_bases
ATACAAATGATACCAACCTTTCATCTATTAATCAAATATTATCTGTGTATTCCCTAACTCCTAGTAAAATCACCTAAAAA
TTTCATATTCAAGGATAGCC

Downstream 100 bases:

>100_bases
ATCCTGGTTTTTATTTAATTTTTGCTATAATTTTTCTTTTAAAAGGATTCTTGCATGCAAAATGGGTATTATGCGGCCAC
GGGAGCTATGGCGGCGCAAT

Product: alpha-ketoglutarate permease

Products: Proton [Cytoplasm]; 2-oxoglutarate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MNPQAQPATKKPLRSLLAASSGNLVEWYDFYAYAFLAPYFAKEFTHTNDPTLALISAFLVFMLGFFMRPLGSLFFGKLGD
KKGRKTSMVYSIILMALGSFMLALLPTKEIVGEWAFLFLLLARLLQGFSVGGEYGVVATYLSELGKNGKKGFYGSFQYVT
LVGGQLLAIFSLFIVENIYTHEQISAFAWRYLFALGGILALLSLFLRNIMEETMDSQTTSKTTIKEETQRGSLKELLNHK
KALMIVFGLTMGGSLCFYTFTVYLKIFLTNSSSFSPKESSFIMLLALSYFIFLQPLCGMLADKIKRTQMLMVFAIAGLIV
TPVVFYGIKHASGVYEALFYEMLALSAMSFYTCIAGVVKAELFPEHVRALGVGLAYAIANALFGGSASYVALQFKQHGFE
WGFVGYVMFSIVIFMVMVIIFPKKTYLE

Sequences:

>Translated_428_residues
MNPQAQPATKKPLRSLLAASSGNLVEWYDFYAYAFLAPYFAKEFTHTNDPTLALISAFLVFMLGFFMRPLGSLFFGKLGD
KKGRKTSMVYSIILMALGSFMLALLPTKEIVGEWAFLFLLLARLLQGFSVGGEYGVVATYLSELGKNGKKGFYGSFQYVT
LVGGQLLAIFSLFIVENIYTHEQISAFAWRYLFALGGILALLSLFLRNIMEETMDSQTTSKTTIKEETQRGSLKELLNHK
KALMIVFGLTMGGSLCFYTFTVYLKIFLTNSSSFSPKESSFIMLLALSYFIFLQPLCGMLADKIKRTQMLMVFAIAGLIV
TPVVFYGIKHASGVYEALFYEMLALSAMSFYTCIAGVVKAELFPEHVRALGVGLAYAIANALFGGSASYVALQFKQHGFE
WGFVGYVMFSIVIFMVMVIIFPKKTYLE
>Mature_428_residues
MNPQAQPATKKPLRSLLAASSGNLVEWYDFYAYAFLAPYFAKEFTHTNDPTLALISAFLVFMLGFFMRPLGSLFFGKLGD
KKGRKTSMVYSIILMALGSFMLALLPTKEIVGEWAFLFLLLARLLQGFSVGGEYGVVATYLSELGKNGKKGFYGSFQYVT
LVGGQLLAIFSLFIVENIYTHEQISAFAWRYLFALGGILALLSLFLRNIMEETMDSQTTSKTTIKEETQRGSLKELLNHK
KALMIVFGLTMGGSLCFYTFTVYLKIFLTNSSSFSPKESSFIMLLALSYFIFLQPLCGMLADKIKRTQMLMVFAIAGLIV
TPVVFYGIKHASGVYEALFYEMLALSAMSFYTCIAGVVKAELFPEHVRALGVGLAYAIANALFGGSASYVALQFKQHGFE
WGFVGYVMFSIVIFMVMVIIFPKKTYLE

Specific function: Uptake of alpha-ketoglutarate across the boundary membrane with the concomitant import of a cation (symport system) [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788942, Length=417, Percent_Identity=46.2829736211031, Blast_Score=345, Evalue=3e-96,
Organism=Escherichia coli, GI1790550, Length=376, Percent_Identity=28.7234042553192, Blast_Score=182, Evalue=4e-47,
Organism=Escherichia coli, GI1788292, Length=384, Percent_Identity=28.3854166666667, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI1789941, Length=399, Percent_Identity=29.0726817042606, Blast_Score=119, Evalue=5e-28,
Organism=Escherichia coli, GI87082404, Length=442, Percent_Identity=24.6606334841629, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI87082231, Length=218, Percent_Identity=28.8990825688073, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 47698; Mature: 47698

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCHHHCC
>Mature Secondary Structure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CCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 2-oxoglutarate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 2-oxoglutarate [Periplasm] = Proton [Cytoplasm] + 2-oxoglutarate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2118499; 9205837; 9278503; 1709493; 2190187; 2053984; 8419306 [H]