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The map label for this gene is cfrBIM [H]

Identifier: 188527117

GI number: 188527117

Start: 313182

End: 314291

Strand: Reverse

Name: cfrBIM [H]

Synonym: HPSH_01620

Alternate gene names: 188527117

Gene position: 314291-313182 (Counterclockwise)

Preceding gene: 188527120

Following gene: 188527116

Centisome position: 19.54

GC content: 35.05

Gene sequence:

>1110_bases
ATGCAGGATTATAGAACTCTTACACTAGAAAATATTTATGCGCCAGATGAGATAACAGACGCGCTTTCATTGTTGCAATC
TTGCGGTATTGAATCGATTGTTAATCCTTCAAATATCACGCTTAATTTTGACATTGTGGATTTAAAAATGCTTGAATCTA
CTACACAAAATACGCTAGTTCAAAAGACATTAGAGGAGTTGTATAAAATTCGCTCATTTTCTAGTCAAAATGGCAAAATC
GTATTTAAGAGCTTCAACAAAGCCAAAAAAGTCGCCAACAAAAAGCAAAACGCAAAGGCAAGATTGGCTTACGAATCTTA
CAAAAAAGAGTTTAGCAAGGAGACAAATGAGATTCAAAGCTGTCTTAATCAAATTTACTGCGAAGATAGCTTGGAGTTTT
TAAAAAAGCTCCCGAATAATTGCATAGATATAGTGCTAACTTCGCCACCTTACAACTTTGGTATCAATTACAGTGCAACG
CAGGATGCAAATCTTTGGCAAGAGTATTTCAACACGCTTTTTGCCATTTTTAAAGAGTGTATTCGTGTATTAAAAAGCGG
AGGGCGAATCATTGTCAATATCCAGCCTATGTTTAGCGATTATATCCCTACACACCATTTTATCAGCAAATTTTTTATTG
ATGAAGGGCTTATTTGGAAAGGCGAGATTTTATGGGAAAAGAATAACTACAACTGCAAATACTGCACTTGGGGAAGCTGG
AAAAGCCCTGCTGCACCATATTTAAAATATTCGTGGGAGTTTATTGAAATTTTTTGCAAAAATAATCTTAAAAAAGAGGG
CGATAAAGACAGCATTGACATAACCGATGATGAATTTAAAAAGTGGGTTTATGGAAAGTGGAACTTTGCCCCAGAACGCA
ACATGAAACAATATGGGCATGATGCAATGTTTCCAGAAGAATTAGTAAAGCGGTGTTTAAAACTATTTTCCTATCAAAAT
GATATTATATTAGATCCATTTAATGGTGCAGGGACAACGACAAAAGTCGCCAAACAACTAGGGCGCAGATTCATAGGTAT
AGACATTAGTGAAAAGTATTGTGAAGTAGCAAGAGCAAGACTAAAAGAAGTAACAAATTTATTTAATTGA

Upstream 100 bases:

>100_bases
CACATCTTTCAACCTTTTAAATCTAAATCATGCTACAATTTTACTGAAAAAACAAGAATAAATCTCGTGCTGTTTAATTG
TTTATTGATTAGGAAATTTA

Downstream 100 bases:

>100_bases
GAGATGTGATTTGAGTTCTATAGAAGATATTATACAAGTCTATGGAAGCGTGGTTAAAGATATTGATAAAGATGCTCAGG
GGCAAGATAGCAGGGCGTAT

Product: DNA methylase

Products: NA

Alternate protein names: M.CfrBI; N(4)- cytosine-specific methyltransferase CfrBI [H]

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MQDYRTLTLENIYAPDEITDALSLLQSCGIESIVNPSNITLNFDIVDLKMLESTTQNTLVQKTLEELYKIRSFSSQNGKI
VFKSFNKAKKVANKKQNAKARLAYESYKKEFSKETNEIQSCLNQIYCEDSLEFLKKLPNNCIDIVLTSPPYNFGINYSAT
QDANLWQEYFNTLFAIFKECIRVLKSGGRIIVNIQPMFSDYIPTHHFISKFFIDEGLIWKGEILWEKNNYNCKYCTWGSW
KSPAAPYLKYSWEFIEIFCKNNLKKEGDKDSIDITDDEFKKWVYGKWNFAPERNMKQYGHDAMFPEELVKRCLKLFSYQN
DIILDPFNGAGTTTKVAKQLGRRFIGIDISEKYCEVARARLKEVTNLFN

Sequences:

>Translated_369_residues
MQDYRTLTLENIYAPDEITDALSLLQSCGIESIVNPSNITLNFDIVDLKMLESTTQNTLVQKTLEELYKIRSFSSQNGKI
VFKSFNKAKKVANKKQNAKARLAYESYKKEFSKETNEIQSCLNQIYCEDSLEFLKKLPNNCIDIVLTSPPYNFGINYSAT
QDANLWQEYFNTLFAIFKECIRVLKSGGRIIVNIQPMFSDYIPTHHFISKFFIDEGLIWKGEILWEKNNYNCKYCTWGSW
KSPAAPYLKYSWEFIEIFCKNNLKKEGDKDSIDITDDEFKKWVYGKWNFAPERNMKQYGHDAMFPEELVKRCLKLFSYQN
DIILDPFNGAGTTTKVAKQLGRRFIGIDISEKYCEVARARLKEVTNLFN
>Mature_369_residues
MQDYRTLTLENIYAPDEITDALSLLQSCGIESIVNPSNITLNFDIVDLKMLESTTQNTLVQKTLEELYKIRSFSSQNGKI
VFKSFNKAKKVANKKQNAKARLAYESYKKEFSKETNEIQSCLNQIYCEDSLEFLKKLPNNCIDIVLTSPPYNFGINYSAT
QDANLWQEYFNTLFAIFKECIRVLKSGGRIIVNIQPMFSDYIPTHHFISKFFIDEGLIWKGEILWEKNNYNCKYCTWGSW
KSPAAPYLKYSWEFIEIFCKNNLKKEGDKDSIDITDDEFKKWVYGKWNFAPERNMKQYGHDAMFPEELVKRCLKLFSYQN
DIILDPFNGAGTTTKVAKQLGRRFIGIDISEKYCEVARARLKEVTNLFN

Specific function: This methylase recognizes the double-stranded sequence CCWWGG, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the CfrBI endonuclease [H]

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR017985
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.113 [H]

Molecular weight: Translated: 43023; Mature: 43023

Theoretical pI: Translated: 8.05; Mature: 8.05

Prosite motif: PS00093 N4_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDYRTLTLENIYAPDEITDALSLLQSCGIESIVNPSNITLNFDIVDLKMLESTTQNTLV
CCCCCEEEHHHCCCCHHHHHHHHHHHHCCHHHCCCCCCEEEEEEEEEHHHHHHHHHHHHH
QKTLEELYKIRSFSSQNGKIVFKSFNKAKKVANKKQNAKARLAYESYKKEFSKETNEIQS
HHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
CLNQIYCEDSLEFLKKLPNNCIDIVLTSPPYNFGINYSATQDANLWQEYFNTLFAIFKEC
HHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
IRVLKSGGRIIVNIQPMFSDYIPTHHFISKFFIDEGLIWKGEILWEKNNYNCKYCTWGSW
HHHHHCCCEEEEEEEHHHHHCCCHHHHHHHHHHCCCCEEECEEEEECCCCCEEEEECCCC
KSPAAPYLKYSWEFIEIFCKNNLKKEGDKDSIDITDDEFKKWVYGKWNFAPERNMKQYGH
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHCCC
DAMFPEELVKRCLKLFSYQNDIILDPFNGAGTTTKVAKQLGRRFIGIDISEKYCEVARAR
CCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHCHHEECCCCHHHHHHHHHHH
LKEVTNLFN
HHHHHHHCC
>Mature Secondary Structure
MQDYRTLTLENIYAPDEITDALSLLQSCGIESIVNPSNITLNFDIVDLKMLESTTQNTLV
CCCCCEEEHHHCCCCHHHHHHHHHHHHCCHHHCCCCCCEEEEEEEEEHHHHHHHHHHHHH
QKTLEELYKIRSFSSQNGKIVFKSFNKAKKVANKKQNAKARLAYESYKKEFSKETNEIQS
HHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
CLNQIYCEDSLEFLKKLPNNCIDIVLTSPPYNFGINYSATQDANLWQEYFNTLFAIFKEC
HHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
IRVLKSGGRIIVNIQPMFSDYIPTHHFISKFFIDEGLIWKGEILWEKNNYNCKYCTWGSW
HHHHHCCCEEEEEEEHHHHHCCCHHHHHHHHHHCCCCEEECEEEEECCCCCEEEEECCCC
KSPAAPYLKYSWEFIEIFCKNNLKKEGDKDSIDITDDEFKKWVYGKWNFAPERNMKQYGH
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHCCC
DAMFPEELVKRCLKLFSYQNDIILDPFNGAGTTTKVAKQLGRRFIGIDISEKYCEVARAR
CCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHCHHEECCCCHHHHHHHHHHH
LKEVTNLFN
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8335262 [H]