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The map label for this gene is ddeIR [H]

Identifier: 188527116

GI number: 188527116

Start: 312479

End: 313171

Strand: Reverse

Name: ddeIR [H]

Synonym: HPSH_01615

Alternate gene names: 188527116

Gene position: 313171-312479 (Counterclockwise)

Preceding gene: 188527117

Following gene: 188527101

Centisome position: 19.47

GC content: 32.76

Gene sequence:

>693_bases
TTGAGTTCTATAGAAGATATTATACAAGTCTATGGAAGCGTGGTTAAAGATATTGATAAAGATGCTCAGGGGCAAGATAG
CAGGGCGTATGGTGGAGTTATAAGAAGTGCTAAAGGCAAATTACTAGAAGATATCAGCGAAGCGATTGTAAAAATAGCGT
GGCAAAATATTGGCGCTAAAGCTAATAGGCTTGAAATAAATTCTAAAAAAATAAAGATTCCTATCAAAGACAGCTACATA
GAAAATATAGCTAACAAACAAGTAAGGGCATACATATTAGAGCGTAAGAAAGATTATTATTACGGATTGAGTGTTGATAA
ACATATATTTGTGGATAATCAATTGGTTATGGGGATAGAGTGTAAAAACTATACGGAAAATGCTATGCTAAAGCGAATAT
TAGTAGATTTTCATCTCCTTAAAACTTTATATCCAAATATATCTTGTTATTTATTTCAGCTAGAAAGTCAATTAGGAGGG
GATTATTCAGCTTTACCAGAAACCCCATTGGGATCAAAATCCACGCATGCTATAATGAGCTACTTTGAAAGCGTGAATCT
GAATATAGTAACCTTACTCAAAGGTGAAAGAGACATAAACCAACCCATACACAAAAATTTCAAACCTTTAGATGAACAGA
TTCTCATTAAAGCAATCAAACTAATGGAGAATGAATTGAAAGTTTATTTGTAG

Upstream 100 bases:

>100_bases
GGGCGCAGATTCATAGGTATAGACATTAGTGAAAAGTATTGTGAAGTAGCAAGAGCAAGACTAAAAGAAGTAACAAATTT
ATTTAATTGAGAGATGTGAT

Downstream 100 bases:

>100_bases
CAGTTGGCGTGAATCCCACTAAATTCACACCAACTTAGGTACAATTTCAACCGCTATTTTTTTCTGGGGCTTCGTTTTAA
GAAATCGTCAGCGATTTCGT

Product: type II restriction enzyme

Products: NA

Alternate protein names: R.DdeI; Endonuclease DdeI; Type II restriction enzyme DdeI [H]

Number of amino acids: Translated: 230; Mature: 229

Protein sequence:

>230_residues
MSSIEDIIQVYGSVVKDIDKDAQGQDSRAYGGVIRSAKGKLLEDISEAIVKIAWQNIGAKANRLEINSKKIKIPIKDSYI
ENIANKQVRAYILERKKDYYYGLSVDKHIFVDNQLVMGIECKNYTENAMLKRILVDFHLLKTLYPNISCYLFQLESQLGG
DYSALPETPLGSKSTHAIMSYFESVNLNIVTLLKGERDINQPIHKNFKPLDEQILIKAIKLMENELKVYL

Sequences:

>Translated_230_residues
MSSIEDIIQVYGSVVKDIDKDAQGQDSRAYGGVIRSAKGKLLEDISEAIVKIAWQNIGAKANRLEINSKKIKIPIKDSYI
ENIANKQVRAYILERKKDYYYGLSVDKHIFVDNQLVMGIECKNYTENAMLKRILVDFHLLKTLYPNISCYLFQLESQLGG
DYSALPETPLGSKSTHAIMSYFESVNLNIVTLLKGERDINQPIHKNFKPLDEQILIKAIKLMENELKVYL
>Mature_229_residues
SSIEDIIQVYGSVVKDIDKDAQGQDSRAYGGVIRSAKGKLLEDISEAIVKIAWQNIGAKANRLEINSKKIKIPIKDSYIE
NIANKQVRAYILERKKDYYYGLSVDKHIFVDNQLVMGIECKNYTENAMLKRILVDFHLLKTLYPNISCYLFQLESQLGGD
YSALPETPLGSKSTHAIMSYFESVNLNIVTLLKGERDINQPIHKNFKPLDEQILIKAIKLMENELKVYL

Specific function: Recognizes the double-stranded sequence CTNAG and cleaves after C-1 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =3.1.21.4 [H]

Molecular weight: Translated: 26219; Mature: 26088

Theoretical pI: Translated: 8.56; Mature: 8.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSIEDIIQVYGSVVKDIDKDAQGQDSRAYGGVIRSAKGKLLEDISEAIVKIAWQNIGAK
CCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
ANRLEINSKKIKIPIKDSYIENIANKQVRAYILERKKDYYYGLSVDKHIFVDNQLVMGIE
CCEEEECCCEEEEEECHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCEEEECCEEEEEEE
CKNYTENAMLKRILVDFHLLKTLYPNISCYLFQLESQLGGDYSALPETPLGSKSTHAIMS
CCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHHCCCCCCCCCCCCCCCHHHHHHH
YFESVNLNIVTLLKGERDINQPIHKNFKPLDEQILIKAIKLMENELKVYL
HHHHCCCEEEEEEECCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SSIEDIIQVYGSVVKDIDKDAQGQDSRAYGGVIRSAKGKLLEDISEAIVKIAWQNIGAK
CHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
ANRLEINSKKIKIPIKDSYIENIANKQVRAYILERKKDYYYGLSVDKHIFVDNQLVMGIE
CCEEEECCCEEEEEECHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCEEEECCEEEEEEE
CKNYTENAMLKRILVDFHLLKTLYPNISCYLFQLESQLGGDYSALPETPLGSKSTHAIMS
CCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHHCCCCCCCCCCCCCCCHHHHHHH
YFESVNLNIVTLLKGERDINQPIHKNFKPLDEQILIKAIKLMENELKVYL
HHHHCCCEEEEEEECCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2823226 [H]