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The map label for this gene is lysS

Identifier: 188526988

GI number: 188526988

Start: 173538

End: 175043

Strand: Direct

Name: lysS

Synonym: HPSH_00925

Alternate gene names: 188526988

Gene position: 173538-175043 (Clockwise)

Preceding gene: 188526987

Following gene: 188526989

Centisome position: 10.79

GC content: 39.84

Gene sequence:

>1506_bases
ATGTTTTCTAACCAATACATCCAACAACGCATCCATAAAGCCAACAGCTTGAGAGAAGAAGGGAAAAACCCTTATAAAAA
CGGCTTGAAACGAAGCCTTACAAACGCCGCTTTTTTAGAAAAATACGCTTATGTTAAGGGTTTAGAAGAGCCTAAAGACA
AGGAAAAATGCGAAAGTATTGTAGGGAGGGTCAAGCTTTTGCGTTTAATGGGTAAGGCTTGTTTTATTAAAATTGAAGAT
GAAAGCGCGATTTTGCAAGCTTATGTTTCGCAAAATGAATTGAACGATGAATTTAAAAGCCTGAAAAAGCATTTAGAAGT
GGGCGATATTGTGTTGGTGAAAGGTTTCCCTTTTGCTACCAAAACCGGTGAATTGAGCGTTCATGCCCTAGAATTTCATA
TTTTAAGCAAAACCATTGTGCCTTTACCCGAAAAATTCCATGGACTGAGCGATATAGAATTGCGTTACCGCCAGCGCTAC
TTGGATTTGATCGTCAATCCTAGCGTTAAAGATGTGTTTAAAAAACGCAGTTTGATTGTTTCTAGCGTGCGGAAATTCTT
TGAAACGGAAGGGTTTTTAGAAGTGGAAACCCCCATGATGCACCCCATTCCTGGCGGGGCGAACGCAAGGCCTTTTATCA
CTTACCATAACGCTTTAGAAATTGAAAGGTATTTAAGAATCGCTCCAGAATTATACCTCAAGCGCTTGATTGTGGGGGGT
TTTGAAGCGGTGTTTGAAATCAATCGCAATTTCAGGAATGAGGGCATGGATCACAGCCATAACCCCGAATTTACGATGAT
TGAGTTTTATTGGGCGTATCACACTTATGAAGATTTGATTGAACTTAGTAAGAGATTATTTGACTACTTGCTAAAGACTT
TGAACTTACCTTCAAAAATCATTTATAACGATATGGAAGTGGATTTCAACCAAACGAGCGTGATTTCCTATTTGGACGCT
TTAGAAACAATAGGGGGCATTAGTAAGGATATTTTAGAAAAAGAAGACAGGCTTTTGGCTTATTTGTTAGAGCAAAGTAT
TAAAGTAGAGCCAGATCTCACTTATGGCAAATTGCTCGCTGAAGCGTTTGATCATTTTGTAGAGCACCAGCTCATTAACC
CCACTTTTGTAACCCAATACCCCATTGAGATTAGCCCCTTAGCCAGACGCAACGATAGTAACCCTAATATTGCTGACAGG
TTTGAATTGTTTATTGCCGGGAAAGAAATCGCTAACGGCTTTAGCGAGTTGAACGATCCTTTAGATCAATTAGAACGCTT
TAAAAATCAAGTGGCTGAAAAAGAAAAAGGCGATGAAGAAGCCCAATACATGGATGAAGATTATGTGTGGGCGTTAGCCC
ATGGAATGCCCCCCACTGCAGGGCAAGGCATAGGCATTGATAGATTAGTGATGCTACTCACTGGGGCTAAAAGCATTAAA
GATGTGATTTTATTCCCAGCGATGCGTCCTGTTAAAAACGATTTTAATGTGGAGAGTGAAGAATAA

Upstream 100 bases:

>100_bases
AAAGGCTAAAGACTTGCCTAAAAACATGCTAGATCCAAAAACTAACCAAACCCCACCAAACCCCACCCCATCTAATAAAG
AACCCCTATAAAGGCATAAC

Downstream 100 bases:

>100_bases
TGGCGTATTTTTTAGAACAAACGGATAGTGAAATTTTTGAGCTTATCTTTGAAGAATACAAGCGGCAAAACGAGCATTTA
GAAATGATAGCGAGCGAGAA

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 501; Mature: 501

Protein sequence:

>501_residues
MFSNQYIQQRIHKANSLREEGKNPYKNGLKRSLTNAAFLEKYAYVKGLEEPKDKEKCESIVGRVKLLRLMGKACFIKIED
ESAILQAYVSQNELNDEFKSLKKHLEVGDIVLVKGFPFATKTGELSVHALEFHILSKTIVPLPEKFHGLSDIELRYRQRY
LDLIVNPSVKDVFKKRSLIVSSVRKFFETEGFLEVETPMMHPIPGGANARPFITYHNALEIERYLRIAPELYLKRLIVGG
FEAVFEINRNFRNEGMDHSHNPEFTMIEFYWAYHTYEDLIELSKRLFDYLLKTLNLPSKIIYNDMEVDFNQTSVISYLDA
LETIGGISKDILEKEDRLLAYLLEQSIKVEPDLTYGKLLAEAFDHFVEHQLINPTFVTQYPIEISPLARRNDSNPNIADR
FELFIAGKEIANGFSELNDPLDQLERFKNQVAEKEKGDEEAQYMDEDYVWALAHGMPPTAGQGIGIDRLVMLLTGAKSIK
DVILFPAMRPVKNDFNVESEE

Sequences:

>Translated_501_residues
MFSNQYIQQRIHKANSLREEGKNPYKNGLKRSLTNAAFLEKYAYVKGLEEPKDKEKCESIVGRVKLLRLMGKACFIKIED
ESAILQAYVSQNELNDEFKSLKKHLEVGDIVLVKGFPFATKTGELSVHALEFHILSKTIVPLPEKFHGLSDIELRYRQRY
LDLIVNPSVKDVFKKRSLIVSSVRKFFETEGFLEVETPMMHPIPGGANARPFITYHNALEIERYLRIAPELYLKRLIVGG
FEAVFEINRNFRNEGMDHSHNPEFTMIEFYWAYHTYEDLIELSKRLFDYLLKTLNLPSKIIYNDMEVDFNQTSVISYLDA
LETIGGISKDILEKEDRLLAYLLEQSIKVEPDLTYGKLLAEAFDHFVEHQLINPTFVTQYPIEISPLARRNDSNPNIADR
FELFIAGKEIANGFSELNDPLDQLERFKNQVAEKEKGDEEAQYMDEDYVWALAHGMPPTAGQGIGIDRLVMLLTGAKSIK
DVILFPAMRPVKNDFNVESEE
>Mature_501_residues
MFSNQYIQQRIHKANSLREEGKNPYKNGLKRSLTNAAFLEKYAYVKGLEEPKDKEKCESIVGRVKLLRLMGKACFIKIED
ESAILQAYVSQNELNDEFKSLKKHLEVGDIVLVKGFPFATKTGELSVHALEFHILSKTIVPLPEKFHGLSDIELRYRQRY
LDLIVNPSVKDVFKKRSLIVSSVRKFFETEGFLEVETPMMHPIPGGANARPFITYHNALEIERYLRIAPELYLKRLIVGG
FEAVFEINRNFRNEGMDHSHNPEFTMIEFYWAYHTYEDLIELSKRLFDYLLKTLNLPSKIIYNDMEVDFNQTSVISYLDA
LETIGGISKDILEKEDRLLAYLLEQSIKVEPDLTYGKLLAEAFDHFVEHQLINPTFVTQYPIEISPLARRNDSNPNIADR
FELFIAGKEIANGFSELNDPLDQLERFKNQVAEKEKGDEEAQYMDEDYVWALAHGMPPTAGQGIGIDRLVMLLTGAKSIK
DVILFPAMRPVKNDFNVESEE

Specific function: Unknown

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI5031815, Length=517, Percent_Identity=39.8452611218569, Blast_Score=367, Evalue=1e-101,
Organism=Homo sapiens, GI194272210, Length=517, Percent_Identity=39.8452611218569, Blast_Score=367, Evalue=1e-101,
Organism=Homo sapiens, GI45439306, Length=462, Percent_Identity=23.3766233766234, Blast_Score=100, Evalue=5e-21,
Organism=Escherichia coli, GI1789256, Length=504, Percent_Identity=50, Blast_Score=479, Evalue=1e-136,
Organism=Escherichia coli, GI1790571, Length=507, Percent_Identity=49.112426035503, Blast_Score=474, Evalue=1e-135,
Organism=Escherichia coli, GI87082379, Length=325, Percent_Identity=32.9230769230769, Blast_Score=155, Evalue=5e-39,
Organism=Escherichia coli, GI1788173, Length=246, Percent_Identity=28.8617886178862, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17535925, Length=518, Percent_Identity=39.7683397683398, Blast_Score=351, Evalue=5e-97,
Organism=Caenorhabditis elegans, GI17535927, Length=518, Percent_Identity=39.7683397683398, Blast_Score=350, Evalue=8e-97,
Organism=Caenorhabditis elegans, GI71994340, Length=513, Percent_Identity=39.9610136452242, Blast_Score=347, Evalue=7e-96,
Organism=Caenorhabditis elegans, GI17551876, Length=350, Percent_Identity=25.1428571428571, Blast_Score=92, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI32566633, Length=266, Percent_Identity=25.187969924812, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6320242, Length=512, Percent_Identity=39.2578125, Blast_Score=347, Evalue=3e-96,
Organism=Saccharomyces cerevisiae, GI6324256, Length=478, Percent_Identity=31.5899581589958, Blast_Score=184, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6323011, Length=484, Percent_Identity=25.2066115702479, Blast_Score=116, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24640851, Length=506, Percent_Identity=40.7114624505929, Blast_Score=364, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24640849, Length=506, Percent_Identity=40.7114624505929, Blast_Score=364, Evalue=1e-101,
Organism=Drosophila melanogaster, GI17136276, Length=474, Percent_Identity=23.6286919831224, Blast_Score=99, Evalue=5e-21,

Paralogues:

None

Copy number: 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 57722; Mature: 57722

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSNQYIQQRIHKANSLREEGKNPYKNGLKRSLTNAAFLEKYAYVKGLEEPKDKEKCESI
CCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
VGRVKLLRLMGKACFIKIEDESAILQAYVSQNELNDEFKSLKKHLEVGDIVLVKGFPFAT
HHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCC
KTGELSVHALEFHILSKTIVPLPEKFHGLSDIELRYRQRYLDLIVNPSVKDVFKKRSLIV
CCCCEEEEEHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
SSVRKFFETEGFLEVETPMMHPIPGGANARPFITYHNALEIERYLRIAPELYLKRLIVGG
HHHHHHHHCCCCEEECCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHH
FEAVFEINRNFRNEGMDHSHNPEFTMIEFYWAYHTYEDLIELSKRLFDYLLKTLNLPSKI
HHHHHHHHHHHHHCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
IYNDMEVDFNQTSVISYLDALETIGGISKDILEKEDRLLAYLLEQSIKVEPDLTYGKLLA
HCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
EAFDHFVEHQLINPTFVTQYPIEISPLARRNDSNPNIADRFELFIAGKEIANGFSELNDP
HHHHHHHHHHCCCCEEEEECCCEECCHHHCCCCCCCHHHHHHHEEECHHHHHHHHHHCCH
LDQLERFKNQVAEKEKGDEEAQYMDEDYVWALAHGMPPTAGQGIGIDRLVMLLTGAKSIK
HHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHCHHHHH
DVILFPAMRPVKNDFNVESEE
HHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MFSNQYIQQRIHKANSLREEGKNPYKNGLKRSLTNAAFLEKYAYVKGLEEPKDKEKCESI
CCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
VGRVKLLRLMGKACFIKIEDESAILQAYVSQNELNDEFKSLKKHLEVGDIVLVKGFPFAT
HHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCC
KTGELSVHALEFHILSKTIVPLPEKFHGLSDIELRYRQRYLDLIVNPSVKDVFKKRSLIV
CCCCEEEEEHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
SSVRKFFETEGFLEVETPMMHPIPGGANARPFITYHNALEIERYLRIAPELYLKRLIVGG
HHHHHHHHCCCCEEECCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHH
FEAVFEINRNFRNEGMDHSHNPEFTMIEFYWAYHTYEDLIELSKRLFDYLLKTLNLPSKI
HHHHHHHHHHHHHCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
IYNDMEVDFNQTSVISYLDALETIGGISKDILEKEDRLLAYLLEQSIKVEPDLTYGKLLA
HCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
EAFDHFVEHQLINPTFVTQYPIEISPLARRNDSNPNIADRFELFIAGKEIANGFSELNDP
HHHHHHHHHHCCCCEEEEECCCEECCHHHCCCCCCCHHHHHHHEEECHHHHHHHHHHCCH
LDQLERFKNQVAEKEKGDEEAQYMDEDYVWALAHGMPPTAGQGIGIDRLVMLLTGAKSIK
HHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHCHHHHH
DVILFPAMRPVKNDFNVESEE
HHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA