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The map label for this gene is prfB

Identifier: 188526975

GI number: 188526975

Start: 162363

End: 163454

Strand: Reverse

Name: prfB

Synonym: HPSH_00860

Alternate gene names: 188526975

Gene position: 163454-162363 (Counterclockwise)

Preceding gene: 188526976

Following gene: 188526974

Centisome position: 10.16

GC content: 41.76

Gene sequence:

>1092_bases
GTGGATAACTACACCTATAGCGAATTGTTAAAAAGCTTGCAAAACAAATGCGATAATATCGCTTTAATCATCAAGCCTGA
AAAGATCAAACAAGAATTAGAACGCATTGAAAAAGAGCAAGAAGACCCTAATTTTTGGCAAGATGTCTTAAAGGCTAGAG
ACACTAATAAAGAAAAAGTGCGCCTAAACCGCTTGCTAGAAACCTATCAAAAAACCAAAAATTCTTTAGATGAAAGCACA
GAATTGTTTGAACTCGCCCAAAACGATAACGATGAGGTTACTTTATCCTTGCTCTATGAAGAGGCTCCTGTTTTAGAGCA
CAGCGTCCAAAAAGTAGAAATTGAAATCATGTTAAGCGGTGAAAACGACGCTTCAAACGCTATTATCACCATTCAACCTG
GAGCGGGGGGGACAGAGAGCCAGGATTGGGCGAGTATTTTGTATCGCATGTATTTGAGGTGGGCAGAAAGAAGGGGCTTT
AAAAGCGAGATTTTAGATTATCAAGATGGCGAAGAAGCGGGCATTAAAGGGGTCGCCTTTATCATTAAGGGCGAAAACGC
TTATGGCTATTTGAAAAATGAAAATGGGGTGCATAGGCTTGTAAGGATTTCGCCCTTTGATGCGAACGCCAAACGGCACA
CGAGTTTTGCGAGCGTGCAAATTAGCCCTGAATTAGACGATGATATTGATATTGAAATTGATGAAAAAGATGTCCGTTAT
GATTATTATAGAGCCAGTGGGGCGGGCGGTCAGCATGTCAATAAAACAGAAAGCGCTGTTAGGATCACGCATTTCCCTAC
CGGTATTGTGGTGCAATGCCAAAACGACAGGAGCCAGCATAAAAACAAAGCGAGCGCGTTAAAAATGCTTAAATCCAAGC
TTTATGAATTGGAATTAGAAAAGCAGCAAAGCACCGCCAAAAATGAAGAAAAAAGCGAGATCGGCTGGGGGCATCAAATA
AGAAGTTATGTCCTAGCCCCCTACCAGCAAGTCAAAGACGCGCGCTCCAATACCGCTTATAGCAATGTGGAAGCGATACT
AGATGGCGATATTGATGCGATTTTAGAAGGCGTTTTGATTGCTAAAGCTTAA

Upstream 100 bases:

>100_bases
AGTTCAGGGCGAAATTGAAATTTTAAGGTTTGAAAATTAAAATGGCTTTTAACAATGCTATAATGGCAAGTTTAAAACCC
CACTCATAAAGGTTCTGTTT

Downstream 100 bases:

>100_bases
GTGCGCTAAAATGACAGCCAATAAATTATGGTTACTATAAAACGCTTTTTAAAACCAATTGGCAAGGAACAACAATGACA
CAAGAAGAATTAGACGCTTT

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MDNYTYSELLKSLQNKCDNIALIIKPEKIKQELERIEKEQEDPNFWQDVLKARDTNKEKVRLNRLLETYQKTKNSLDEST
ELFELAQNDNDEVTLSLLYEEAPVLEHSVQKVEIEIMLSGENDASNAIITIQPGAGGTESQDWASILYRMYLRWAERRGF
KSEILDYQDGEEAGIKGVAFIIKGENAYGYLKNENGVHRLVRISPFDANAKRHTSFASVQISPELDDDIDIEIDEKDVRY
DYYRASGAGGQHVNKTESAVRITHFPTGIVVQCQNDRSQHKNKASALKMLKSKLYELELEKQQSTAKNEEKSEIGWGHQI
RSYVLAPYQQVKDARSNTAYSNVEAILDGDIDAILEGVLIAKA

Sequences:

>Translated_363_residues
MDNYTYSELLKSLQNKCDNIALIIKPEKIKQELERIEKEQEDPNFWQDVLKARDTNKEKVRLNRLLETYQKTKNSLDEST
ELFELAQNDNDEVTLSLLYEEAPVLEHSVQKVEIEIMLSGENDASNAIITIQPGAGGTESQDWASILYRMYLRWAERRGF
KSEILDYQDGEEAGIKGVAFIIKGENAYGYLKNENGVHRLVRISPFDANAKRHTSFASVQISPELDDDIDIEIDEKDVRY
DYYRASGAGGQHVNKTESAVRITHFPTGIVVQCQNDRSQHKNKASALKMLKSKLYELELEKQQSTAKNEEKSEIGWGHQI
RSYVLAPYQQVKDARSNTAYSNVEAILDGDIDAILEGVLIAKA
>Mature_363_residues
MDNYTYSELLKSLQNKCDNIALIIKPEKIKQELERIEKEQEDPNFWQDVLKARDTNKEKVRLNRLLETYQKTKNSLDEST
ELFELAQNDNDEVTLSLLYEEAPVLEHSVQKVEIEIMLSGENDASNAIITIQPGAGGTESQDWASILYRMYLRWAERRGF
KSEILDYQDGEEAGIKGVAFIIKGENAYGYLKNENGVHRLVRISPFDANAKRHTSFASVQISPELDDDIDIEIDEKDVRY
DYYRASGAGGQHVNKTESAVRITHFPTGIVVQCQNDRSQHKNKASALKMLKSKLYELELEKQQSTAKNEEKSEIGWGHQI
RSYVLAPYQQVKDARSNTAYSNVEAILDGDIDAILEGVLIAKA

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=277, Percent_Identity=36.8231046931408, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI34577120, Length=324, Percent_Identity=31.4814814814815, Blast_Score=152, Evalue=7e-37,
Organism=Homo sapiens, GI166795305, Length=191, Percent_Identity=36.6492146596859, Blast_Score=120, Evalue=3e-27,
Organism=Escherichia coli, GI2367172, Length=354, Percent_Identity=49.7175141242938, Blast_Score=352, Evalue=2e-98,
Organism=Escherichia coli, GI1787462, Length=241, Percent_Identity=39.8340248962656, Blast_Score=193, Evalue=1e-50,
Organism=Caenorhabditis elegans, GI17542784, Length=280, Percent_Identity=29.6428571428571, Blast_Score=105, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6321295, Length=327, Percent_Identity=31.1926605504587, Blast_Score=141, Evalue=2e-34,
Organism=Drosophila melanogaster, GI19921226, Length=211, Percent_Identity=36.9668246445498, Blast_Score=149, Evalue=4e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 41327; Mature: 41327

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNYTYSELLKSLQNKCDNIALIIKPEKIKQELERIEKEQEDPNFWQDVLKARDTNKEKV
CCCCCHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHH
RLNRLLETYQKTKNSLDESTELFELAQNDNDEVTLSLLYEEAPVLEHSVQKVEIEIMLSG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHCCEEEEEEEEEEC
ENDASNAIITIQPGAGGTESQDWASILYRMYLRWAERRGFKSEILDYQDGEEAGIKGVAF
CCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCEEEE
IIKGENAYGYLKNENGVHRLVRISPFDANAKRHTSFASVQISPELDDDIDIEIDEKDVRY
EEECCCCEEEEECCCCCEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCEEEECCCCCEE
DYYRASGAGGQHVNKTESAVRITHFPTGIVVQCQNDRSQHKNKASALKMLKSKLYELELE
EEEECCCCCCCCCCCCCCEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
KQQSTAKNEEKSEIGWGHQIRSYVLAPYQQVKDARSNTAYSNVEAILDGDIDAILEGVLI
HHHHHHCCCHHHHCCCHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHH
AKA
CCC
>Mature Secondary Structure
MDNYTYSELLKSLQNKCDNIALIIKPEKIKQELERIEKEQEDPNFWQDVLKARDTNKEKV
CCCCCHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHH
RLNRLLETYQKTKNSLDESTELFELAQNDNDEVTLSLLYEEAPVLEHSVQKVEIEIMLSG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHCCEEEEEEEEEEC
ENDASNAIITIQPGAGGTESQDWASILYRMYLRWAERRGFKSEILDYQDGEEAGIKGVAF
CCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCEEEE
IIKGENAYGYLKNENGVHRLVRISPFDANAKRHTSFASVQISPELDDDIDIEIDEKDVRY
EEECCCCEEEEECCCCCEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCEEEECCCCCEE
DYYRASGAGGQHVNKTESAVRITHFPTGIVVQCQNDRSQHKNKASALKMLKSKLYELELE
EEEECCCCCCCCCCCCCCEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
KQQSTAKNEEKSEIGWGHQIRSYVLAPYQQVKDARSNTAYSNVEAILDGDIDAILEGVLI
HHHHHHCCCHHHHCCCHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHH
AKA
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA