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The map label for this gene is ppsA [H]

Identifier: 188526924

GI number: 188526924

Start: 117391

End: 119829

Strand: Reverse

Name: ppsA [H]

Synonym: HPSH_00605

Alternate gene names: 188526924

Gene position: 119829-117391 (Counterclockwise)

Preceding gene: 188526935

Following gene: 188526923

Centisome position: 7.45

GC content: 42.31

Gene sequence:

>2439_bases
GTGCGATATATCAAGTTTTTCAAAGAGTTGAACAATAAGAATGTGAATCTGGTTGGGGGCAAGAACGCTAGCATTGGTGA
AATGTTTCAAGAATTAGTGCCTATTGGCATTAAAGTGCCTGATGGCTTTGCGATCACAAGCGAAGCGTATTGGTATCTTT
TAGAGCAAGGGGGGGCTAAACAAAAAATCATAGAGCTTTTAGAAAATGTTGATGCCACGGAAATTGACGTGTTAAAAATC
CGCTCCAAACAAATCAGAGAGCTTATTTTTGGCACGCCTTTTCCTAGCGATTTAAGAGATGAGATTTTTCAAGCTTATGA
AATTTTAAGCCAGCAATACCACATGAAAGAAGCCGATGTGGCCGTAAGAAGCTCCGCTACCGCAGAAGATTTGCCGGACG
CTTCTTTTGCCGGGCAGCAAGACACTTATTTAAACATTAAGGGTAAAACAGAATTGATCCACTATATCAAATCCTGTTTA
GCGTCGCTTTTTACCGATAGAGCGATTAGTTATAGAGCGAGTCGTGGGTTTGATCATTTAAAAGTCGCCTTGAGCGTGGG
GGTGCAAAAAATGGTGCGAGCGGATAAAGGCAGCGCGGGCGTGATGTTTTCTATTGACACCGAAACCGGTTTTAAAGACG
CGGTGTTTATCACTTCAGCGTGGGGGTTAGGCGAAAATGTGGTGGGCGGCACGATAAACCCTGATGAATTTTATGTGTTT
AAGCCCACTTTAGAGCAAAACAAACGCCCCATTATCAAACGCCAACTCGGCAATAAAACGCAAAAAATGGTCTATGCCCC
AAGAGGTAGCGAACACCCCACCAGAAACATTAAAACCACCAAAAAAGAATGGCAATCCTTTTCATTGAGCGATGAAGATG
TGCTGATTTTAGCCAAATACGCCATTGAGATTGAAAAACATTACTCTAAAGAAGCCAAACAATACCGCCCTATGGATATA
GAATGGGCTAAAGATGGCGATAGCGGGGAAATCTTTATCGTTCAAGCGCGCCCAGAAACCGTTCAAAGCCAAAAAACCAA
AGAAGAAAGTCAAGTCTTTGAAAAATTCAAATTCAAAAACCCTAACGAAAAGAAAGAGATCATCTTACAAGGCAGAGCGA
TTGGGAGTAAAATTGGCTCAGGAAAAGTGCGCATCATCAATGATTTGGAGCACATGAATTCTTTTAAAGAGGGCGAAATT
TTAGTTACGGATAACACCGACCCAGATTGGGAGCCTTGCATGAAAAAAGCGAGCGCGGTTATCACTAATCGTGGAGGGCG
CACTTGCCATGCCGCTATTGTGGCTAGAGAAATTGGCGTGCCAGCTATTGTTGGGGTGAGCGGGGCGACTGATAGCCTTT
ATACCGGCATGGAAATCACGGTTTCTTGCGCTGAGGGTGAAGAGGGCTATGTGTATGCGGGCGTTTATGAGCATGAAATT
GAAAGGGTGGAGCTTTCTAACATGCAAGAAACTCAAACAAAAATTTACATCAATATTGGAAACCCTGAAAAAGCCTTTGG
CTTTTCTCAGCTCCCTAATCACGGCGTAGGGCTAGCTAGAATGGAAATGATTATTTTAAATCAAATCAAAGCTCACCCCT
TAGCCTTAGTGGATTTGCACCACAAAAAAAGCGTGAAAGAAAAAAATGAAATTGAAAACCTTATGGCAGGCTATGCTAAC
CCTAAAGATTTTTTTGTGAAAAAAATCGCTGAAGGCATTGGCATGATCAGTGCAGCGTTTTACCCTAAACCTGTCATTGT
GAGAACGAGCGATTTCAAATCCAATGAATACATGCGCATGCTTGGCGGCTCTAGCTATGAGCCTAATGAAGAAAACCCCA
TGCTTGGCTATAGGGGGGCTAGTCGGTATTATTCAGAGAGCTATAATGAAGCGTTTTCGTGGGAGTGTGAAGCTTTAGCG
TTAGTGAGGGAAGAAATGGGCTTGACGAACATGAAAGTGATGATCCCTTTTTTGCGAACCATTGAAGAGGGTAAAAAAGT
CCTAGAAATCTTAAGAAAAAACAATCTAGAATCCGGTAAAAACGGGCTTGAAATTTATATCATGTGCGAATTACCGGTGA
ATGTCATTTTGGCTGATGATTTCTTAAGCTTGTTTGATGGCTTTTCTATTGGCTCTAATGATTTAACCCAGCTCACTTTA
GGCGTGGATAGAGACAGCGAATTAGTCAGCCATGTCTTTGATGAAAGGAATGAAGCGATGCTAAAAATGTTTAAAAAAGC
GATTGAAGCTTGCAAAAGGCACAACAAATATTGCGGGATTTGCGGGCAAGCCCCAAGCGATTACCCTGAAGTAACAGAGT
TTTTAGTCAAAGAGGGCATCACTTCCATTTCTTTAAACCCTGATAGCGTGATCCCCACTTGGAACGCTGTAGCCAAGTTA
GAAAAAGAATTGAAAGACCATGGCTTAACTGCGCATTGA

Upstream 100 bases:

>100_bases
AAAGCTCAGTAATACAAACTAGCTAAAACTAAAGCTCTATCATGCTATCATTAATATATTTTTGTTTTAAGAAACTTAAT
GATAATTTTAAGGGAGTTTT

Downstream 100 bases:

>100_bases
TAATGAAGGGGTTGATCTAACTTGAGTGGATTTTTTCGTATTAGTTTCCACAGATTTAATTTTGATGGGTAAGATTGTTT
TTTGAAAAAAAGGGTGGTAA

Product: phosphoenolpyruvate synthase

Products: NA

Alternate protein names: PEP synthase; Pyruvate, water dikinase [H]

Number of amino acids: Translated: 812; Mature: 812

Protein sequence:

>812_residues
MRYIKFFKELNNKNVNLVGGKNASIGEMFQELVPIGIKVPDGFAITSEAYWYLLEQGGAKQKIIELLENVDATEIDVLKI
RSKQIRELIFGTPFPSDLRDEIFQAYEILSQQYHMKEADVAVRSSATAEDLPDASFAGQQDTYLNIKGKTELIHYIKSCL
ASLFTDRAISYRASRGFDHLKVALSVGVQKMVRADKGSAGVMFSIDTETGFKDAVFITSAWGLGENVVGGTINPDEFYVF
KPTLEQNKRPIIKRQLGNKTQKMVYAPRGSEHPTRNIKTTKKEWQSFSLSDEDVLILAKYAIEIEKHYSKEAKQYRPMDI
EWAKDGDSGEIFIVQARPETVQSQKTKEESQVFEKFKFKNPNEKKEIILQGRAIGSKIGSGKVRIINDLEHMNSFKEGEI
LVTDNTDPDWEPCMKKASAVITNRGGRTCHAAIVAREIGVPAIVGVSGATDSLYTGMEITVSCAEGEEGYVYAGVYEHEI
ERVELSNMQETQTKIYINIGNPEKAFGFSQLPNHGVGLARMEMIILNQIKAHPLALVDLHHKKSVKEKNEIENLMAGYAN
PKDFFVKKIAEGIGMISAAFYPKPVIVRTSDFKSNEYMRMLGGSSYEPNEENPMLGYRGASRYYSESYNEAFSWECEALA
LVREEMGLTNMKVMIPFLRTIEEGKKVLEILRKNNLESGKNGLEIYIMCELPVNVILADDFLSLFDGFSIGSNDLTQLTL
GVDRDSELVSHVFDERNEAMLKMFKKAIEACKRHNKYCGICGQAPSDYPEVTEFLVKEGITSISLNPDSVIPTWNAVAKL
EKELKDHGLTAH

Sequences:

>Translated_812_residues
MRYIKFFKELNNKNVNLVGGKNASIGEMFQELVPIGIKVPDGFAITSEAYWYLLEQGGAKQKIIELLENVDATEIDVLKI
RSKQIRELIFGTPFPSDLRDEIFQAYEILSQQYHMKEADVAVRSSATAEDLPDASFAGQQDTYLNIKGKTELIHYIKSCL
ASLFTDRAISYRASRGFDHLKVALSVGVQKMVRADKGSAGVMFSIDTETGFKDAVFITSAWGLGENVVGGTINPDEFYVF
KPTLEQNKRPIIKRQLGNKTQKMVYAPRGSEHPTRNIKTTKKEWQSFSLSDEDVLILAKYAIEIEKHYSKEAKQYRPMDI
EWAKDGDSGEIFIVQARPETVQSQKTKEESQVFEKFKFKNPNEKKEIILQGRAIGSKIGSGKVRIINDLEHMNSFKEGEI
LVTDNTDPDWEPCMKKASAVITNRGGRTCHAAIVAREIGVPAIVGVSGATDSLYTGMEITVSCAEGEEGYVYAGVYEHEI
ERVELSNMQETQTKIYINIGNPEKAFGFSQLPNHGVGLARMEMIILNQIKAHPLALVDLHHKKSVKEKNEIENLMAGYAN
PKDFFVKKIAEGIGMISAAFYPKPVIVRTSDFKSNEYMRMLGGSSYEPNEENPMLGYRGASRYYSESYNEAFSWECEALA
LVREEMGLTNMKVMIPFLRTIEEGKKVLEILRKNNLESGKNGLEIYIMCELPVNVILADDFLSLFDGFSIGSNDLTQLTL
GVDRDSELVSHVFDERNEAMLKMFKKAIEACKRHNKYCGICGQAPSDYPEVTEFLVKEGITSISLNPDSVIPTWNAVAKL
EKELKDHGLTAH
>Mature_812_residues
MRYIKFFKELNNKNVNLVGGKNASIGEMFQELVPIGIKVPDGFAITSEAYWYLLEQGGAKQKIIELLENVDATEIDVLKI
RSKQIRELIFGTPFPSDLRDEIFQAYEILSQQYHMKEADVAVRSSATAEDLPDASFAGQQDTYLNIKGKTELIHYIKSCL
ASLFTDRAISYRASRGFDHLKVALSVGVQKMVRADKGSAGVMFSIDTETGFKDAVFITSAWGLGENVVGGTINPDEFYVF
KPTLEQNKRPIIKRQLGNKTQKMVYAPRGSEHPTRNIKTTKKEWQSFSLSDEDVLILAKYAIEIEKHYSKEAKQYRPMDI
EWAKDGDSGEIFIVQARPETVQSQKTKEESQVFEKFKFKNPNEKKEIILQGRAIGSKIGSGKVRIINDLEHMNSFKEGEI
LVTDNTDPDWEPCMKKASAVITNRGGRTCHAAIVAREIGVPAIVGVSGATDSLYTGMEITVSCAEGEEGYVYAGVYEHEI
ERVELSNMQETQTKIYINIGNPEKAFGFSQLPNHGVGLARMEMIILNQIKAHPLALVDLHHKKSVKEKNEIENLMAGYAN
PKDFFVKKIAEGIGMISAAFYPKPVIVRTSDFKSNEYMRMLGGSSYEPNEENPMLGYRGASRYYSESYNEAFSWECEALA
LVREEMGLTNMKVMIPFLRTIEEGKKVLEILRKNNLESGKNGLEIYIMCELPVNVILADDFLSLFDGFSIGSNDLTQLTL
GVDRDSELVSHVFDERNEAMLKMFKKAIEACKRHNKYCGICGQAPSDYPEVTEFLVKEGITSISLNPDSVIPTWNAVAKL
EKELKDHGLTAH

Specific function: Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=800, Percent_Identity=52.5, Blast_Score=872, Evalue=0.0,
Organism=Escherichia coli, GI1788756, Length=428, Percent_Identity=26.8691588785047, Blast_Score=122, Evalue=1e-28,
Organism=Escherichia coli, GI48994992, Length=373, Percent_Identity=28.1501340482574, Blast_Score=108, Evalue=2e-24,
Organism=Escherichia coli, GI1788726, Length=376, Percent_Identity=25.2659574468085, Blast_Score=97, Evalue=4e-21,
Organism=Escherichia coli, GI1789193, Length=426, Percent_Identity=23.943661971831, Blast_Score=93, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17564524, Length=239, Percent_Identity=27.6150627615063, Blast_Score=80, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR006319
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.2 [H]

Molecular weight: Translated: 91188; Mature: 91188

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYIKFFKELNNKNVNLVGGKNASIGEMFQELVPIGIKVPDGFAITSEAYWYLLEQGGAK
CHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHCCCCEECCCCEEECCCCEEEEEECCCCH
QKIIELLENVDATEIDVLKIRSKQIRELIFGTPFPSDLRDEIFQAYEILSQQYHMKEADV
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHH
AVRSSATAEDLPDASFAGQQDTYLNIKGKTELIHYIKSCLASLFTDRAISYRASRGFDHL
HHHCCCCHHCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
KVALSVGVQKMVRADKGSAGVMFSIDTETGFKDAVFITSAWGLGENVVGGTINPDEFYVF
HHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEE
KPTLEQNKRPIIKRQLGNKTQKMVYAPRGSEHPTRNIKTTKKEWQSFSLSDEDVLILAKY
CCCCCCCCCCHHHHHHCCCCCEEEECCCCCCCCCCCCCHHHHHHHCCCCCCCCEEEEEHH
AIEIEKHYSKEAKQYRPMDIEWAKDGDSGEIFIVQARPETVQSQKTKEESQVFEKFKFKN
HHHHHHHHHHHHHHCCCCCEEECCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCC
PNEKKEIILQGRAIGSKIGSGKVRIINDLEHMNSFKEGEILVTDNTDPDWEPCMKKASAV
CCCCHHEEEECCHHCCCCCCCCEEEEECHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHH
ITNRGGRTCHAAIVAREIGVPAIVGVSGATDSLYTGMEITVSCAEGEEGYVYAGVYEHEI
EECCCCCEEHHHHHHHHCCCCEEEECCCCCCHHCCCEEEEEEECCCCCCEEEEEEEHHHH
ERVELSNMQETQTKIYINIGNPEKAFGFSQLPNHGVGLARMEMIILNQIKAHPLALVDLH
HHHHHCCCCCCCEEEEEEECCCHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEH
HKKSVKEKNEIENLMAGYANPKDFFVKKIAEGIGMISAAFYPKPVIVRTSDFKSNEYMRM
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHH
LGGSSYEPNEENPMLGYRGASRYYSESYNEAFSWECEALALVREEMGLTNMKVMIPFLRT
HCCCCCCCCCCCCCEECCCCHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHH
IEEGKKVLEILRKNNLESGKNGLEIYIMCELPVNVILADDFLSLFDGFSIGSNDLTQLTL
HHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCEEEEHHHHHHHHCCCCCCCCCCEEEEE
GVDRDSELVSHVFDERNEAMLKMFKKAIEACKRHNKYCGICGQAPSDYPEVTEFLVKEGI
ECCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHCCC
TSISLNPDSVIPTWNAVAKLEKELKDHGLTAH
CEEECCCCCCCCCHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MRYIKFFKELNNKNVNLVGGKNASIGEMFQELVPIGIKVPDGFAITSEAYWYLLEQGGAK
CHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHCCCCEECCCCEEECCCCEEEEEECCCCH
QKIIELLENVDATEIDVLKIRSKQIRELIFGTPFPSDLRDEIFQAYEILSQQYHMKEADV
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHH
AVRSSATAEDLPDASFAGQQDTYLNIKGKTELIHYIKSCLASLFTDRAISYRASRGFDHL
HHHCCCCHHCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
KVALSVGVQKMVRADKGSAGVMFSIDTETGFKDAVFITSAWGLGENVVGGTINPDEFYVF
HHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEE
KPTLEQNKRPIIKRQLGNKTQKMVYAPRGSEHPTRNIKTTKKEWQSFSLSDEDVLILAKY
CCCCCCCCCCHHHHHHCCCCCEEEECCCCCCCCCCCCCHHHHHHHCCCCCCCCEEEEEHH
AIEIEKHYSKEAKQYRPMDIEWAKDGDSGEIFIVQARPETVQSQKTKEESQVFEKFKFKN
HHHHHHHHHHHHHHCCCCCEEECCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCC
PNEKKEIILQGRAIGSKIGSGKVRIINDLEHMNSFKEGEILVTDNTDPDWEPCMKKASAV
CCCCHHEEEECCHHCCCCCCCCEEEEECHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHH
ITNRGGRTCHAAIVAREIGVPAIVGVSGATDSLYTGMEITVSCAEGEEGYVYAGVYEHEI
EECCCCCEEHHHHHHHHCCCCEEEECCCCCCHHCCCEEEEEEECCCCCCEEEEEEEHHHH
ERVELSNMQETQTKIYINIGNPEKAFGFSQLPNHGVGLARMEMIILNQIKAHPLALVDLH
HHHHHCCCCCCCEEEEEEECCCHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEH
HKKSVKEKNEIENLMAGYANPKDFFVKKIAEGIGMISAAFYPKPVIVRTSDFKSNEYMRM
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHH
LGGSSYEPNEENPMLGYRGASRYYSESYNEAFSWECEALALVREEMGLTNMKVMIPFLRT
HCCCCCCCCCCCCCEECCCCHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHH
IEEGKKVLEILRKNNLESGKNGLEIYIMCELPVNVILADDFLSLFDGFSIGSNDLTQLTL
HHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCEEEEHHHHHHHHCCCCCCCCCCEEEEE
GVDRDSELVSHVFDERNEAMLKMFKKAIEACKRHNKYCGICGQAPSDYPEVTEFLVKEGI
ECCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHCCC
TSISLNPDSVIPTWNAVAKLEKELKDHGLTAH
CEEECCCCCCCCCHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]