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The map label for this gene is yfkN [H]

Identifier: 188526909

GI number: 188526909

Start: 98036

End: 99781

Strand: Reverse

Name: yfkN [H]

Synonym: HPSH_00520

Alternate gene names: 188526909

Gene position: 99781-98036 (Counterclockwise)

Preceding gene: 188526910

Following gene: 188526908

Centisome position: 6.2

GC content: 39.23

Gene sequence:

>1746_bases
ATGAAAAAATTGGTTTTAATCGTCTTTTTAACGCTAACGCTTTCAATATCTGCAAAAGAAGTGAAAATCGTGTTTTTAGA
AACTTCAGACATTCATGGGCGGCTTTTTTCGTATGATTATGCGACTGGCGAGCAAAAACCCAATAATGGCTTGACAAGGA
TTGCGGCTTTAATCAAAAAGCAGAGGGCTGAAAATAAAAATGTGGTTTTGATTGACAGCGGGGATTTGTTGCAGGGTAAT
AGCGCGGAGTTGTTTAATGATGAGCCTATCCACCCATTAGTTTTAGCCGAAAATGATTTGAAATTTGATATTCGTGTGCT
TGGGAATCACGAGTTTAATTTCAGTAAAGATTTTTTAGAGAAAAATATCAAGGGGTTTAATGGCGATGTCGTGAATGCAA
ATATCATCAAAACTGCGGACAATCAGCCGTTTGTAAAGCCTTATGCGATCAAGAAAATTGATGGCGTGAGGGTGGCAATT
GTGGGGTATGTGGTGCCACACATCCCCACTTGGGAAGCCTCTACGCCTGAACATTTTGCGGGCTTAGAGTTTTTGGACGC
TGAAGAAGCGTTAAAAAAGACCTTAAAAGAGCTAAAAGGGAAGTATGATATTTTGATTGGCGCTTTTCATTTGGGGCGAG
AAGATGAGAAAGGTGGCGATGGAGTCCCTGATCTGGCGAAAAAATTCCCGCAATTTGACATCATTTTTGCAGGGCATGAG
CATGCGGTTTATAACACCAAAATAGGAAAGGTGCACACCATTGAGCCTGGAGCGTATGGGGCTTATCTGGCAAAAGGCGT
GGTAATGTTTGATACCAAAACGAAGAAAAAAATCGTAACGACTGAAAATTTACCCACAAAAGGCGTGCCAGAAGATGAAG
AATTAGCGAAAAAATACGAATATGTGGATAAAAAATCAAAAGAATACGCTAATGAAGTTGTCGGCGAAGTTACAAAAACC
TTTATTGACAGGCCTGATTTTATCACAGGAGAAGAAAAAATCACCACGATGCCCACCGCCGCATTGCAAGAAACACCGGT
GATAGAATTGATTAATATTGTGCAAAAATATTATGCAAAAGCCGATGTTTCAGCGGCAGCCTTATTCAATTTTGGCGCCA
ATTTGAAAAAAGGGCCTTTCAAAAGAAAAGATGTCGCCTATATTTACAAGTTCGCTAATACGCTCATTGGAGTGAAAATA
ACGGGTGAAAATCTGTTGAAATACATGGAATGGTCGTATCAATTCTACAATCAGTTGCAACCCGGAGATTTGACGATCAG
CTTTAATGAAAATATTCGCGGCTATAACTTTGATATGTTTTCTGGCGTGAAATACCAAGTTGATGTTACAAAACCCGCCG
GATCAAGGATTATTAATCCGACAATCAACAACAAACCCATTGACCCCAAAGCGGTCTATAAATTAGCGATCAACAATTAC
CGATTTGGGACATTATCCAAGACATTAAATTTGGTTACAGACGCTAATAGGTATTATAATTCTTACGATGGATTGCAAGA
TAATGGGCAGATACGAGATTTGATCATCAAATACATCACGGAAGAAAAAGGCGGGAAGGTAACCCCTGAACTGGAACATA
ACTGGGAAATCATCAACTATAATTTCAAAAACCCGTTGCTGGAAAAATTGAGAGAAAAATTAAAAGAGGGGAGCATCAAA
ATCCCCACTTCAAAAGACGGGAGGACTTTGAATGTCAAATCCATTAAGGAAAGTGAAGTTAAATAA

Upstream 100 bases:

>100_bases
TTACCCGTTTTAGCGTTACATTCCAATCAAAAACCATCGTATCAGTGTTAATATTATGTAAAAATTAATGTTATGAATTT
CTTGTATTAGAAGGATTTCA

Downstream 100 bases:

>100_bases
GATTTTTTATTTTTATTATTTTACTCTTAAGTTTAATTTAAAAAAGGGTGTTCTATAATCCTAGATTGTTGGCTTTTTAC
TTTTTTGGCGGTTTTCAAAC

Product: 2',3'-cyclic-nucleotide 2'-phosphodiesterase

Products: NA

Alternate protein names: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase; 5'-nucleotidase [H]

Number of amino acids: Translated: 581; Mature: 581

Protein sequence:

>581_residues
MKKLVLIVFLTLTLSISAKEVKIVFLETSDIHGRLFSYDYATGEQKPNNGLTRIAALIKKQRAENKNVVLIDSGDLLQGN
SAELFNDEPIHPLVLAENDLKFDIRVLGNHEFNFSKDFLEKNIKGFNGDVVNANIIKTADNQPFVKPYAIKKIDGVRVAI
VGYVVPHIPTWEASTPEHFAGLEFLDAEEALKKTLKELKGKYDILIGAFHLGREDEKGGDGVPDLAKKFPQFDIIFAGHE
HAVYNTKIGKVHTIEPGAYGAYLAKGVVMFDTKTKKKIVTTENLPTKGVPEDEELAKKYEYVDKKSKEYANEVVGEVTKT
FIDRPDFITGEEKITTMPTAALQETPVIELINIVQKYYAKADVSAAALFNFGANLKKGPFKRKDVAYIYKFANTLIGVKI
TGENLLKYMEWSYQFYNQLQPGDLTISFNENIRGYNFDMFSGVKYQVDVTKPAGSRIINPTINNKPIDPKAVYKLAINNY
RFGTLSKTLNLVTDANRYYNSYDGLQDNGQIRDLIIKYITEEKGGKVTPELEHNWEIINYNFKNPLLEKLREKLKEGSIK
IPTSKDGRTLNVKSIKESEVK

Sequences:

>Translated_581_residues
MKKLVLIVFLTLTLSISAKEVKIVFLETSDIHGRLFSYDYATGEQKPNNGLTRIAALIKKQRAENKNVVLIDSGDLLQGN
SAELFNDEPIHPLVLAENDLKFDIRVLGNHEFNFSKDFLEKNIKGFNGDVVNANIIKTADNQPFVKPYAIKKIDGVRVAI
VGYVVPHIPTWEASTPEHFAGLEFLDAEEALKKTLKELKGKYDILIGAFHLGREDEKGGDGVPDLAKKFPQFDIIFAGHE
HAVYNTKIGKVHTIEPGAYGAYLAKGVVMFDTKTKKKIVTTENLPTKGVPEDEELAKKYEYVDKKSKEYANEVVGEVTKT
FIDRPDFITGEEKITTMPTAALQETPVIELINIVQKYYAKADVSAAALFNFGANLKKGPFKRKDVAYIYKFANTLIGVKI
TGENLLKYMEWSYQFYNQLQPGDLTISFNENIRGYNFDMFSGVKYQVDVTKPAGSRIINPTINNKPIDPKAVYKLAINNY
RFGTLSKTLNLVTDANRYYNSYDGLQDNGQIRDLIIKYITEEKGGKVTPELEHNWEIINYNFKNPLLEKLREKLKEGSIK
IPTSKDGRTLNVKSIKESEVK
>Mature_581_residues
MKKLVLIVFLTLTLSISAKEVKIVFLETSDIHGRLFSYDYATGEQKPNNGLTRIAALIKKQRAENKNVVLIDSGDLLQGN
SAELFNDEPIHPLVLAENDLKFDIRVLGNHEFNFSKDFLEKNIKGFNGDVVNANIIKTADNQPFVKPYAIKKIDGVRVAI
VGYVVPHIPTWEASTPEHFAGLEFLDAEEALKKTLKELKGKYDILIGAFHLGREDEKGGDGVPDLAKKFPQFDIIFAGHE
HAVYNTKIGKVHTIEPGAYGAYLAKGVVMFDTKTKKKIVTTENLPTKGVPEDEELAKKYEYVDKKSKEYANEVVGEVTKT
FIDRPDFITGEEKITTMPTAALQETPVIELINIVQKYYAKADVSAAALFNFGANLKKGPFKRKDVAYIYKFANTLIGVKI
TGENLLKYMEWSYQFYNQLQPGDLTISFNENIRGYNFDMFSGVKYQVDVTKPAGSRIINPTINNKPIDPKAVYKLAINNY
RFGTLSKTLNLVTDANRYYNSYDGLQDNGQIRDLIIKYITEEKGGKVTPELEHNWEIINYNFKNPLLEKLREKLKEGSIK
IPTSKDGRTLNVKSIKESEVK

Specific function: Catalyzes the release of inorganic phosphate from 2',3'- cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'- nucleotidase activity. Does not catalyze the release of inorganic phospha

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Secreted, cell wall; Peptidoglycan-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Escherichia coli, GI1790658, Length=616, Percent_Identity=28.5714285714286, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI1786687, Length=527, Percent_Identity=21.8216318785579, Blast_Score=80, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24641187, Length=505, Percent_Identity=20.5940594059406, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR001899 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16; =3.1.3.5 [H]

Molecular weight: Translated: 65536; Mature: 65536

Theoretical pI: Translated: 9.04; Mature: 9.04

Prosite motif: PS00786 5_NUCLEOTIDASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLVLIVFLTLTLSISAKEVKIVFLETSDIHGRLFSYDYATGEQKPNNGLTRIAALIKK
CCCEEEEEEEHHHHEEECCEEEEEEEECCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHH
QRAENKNVVLIDSGDLLQGNSAELFNDEPIHPLVLAENDLKFDIRVLGNHEFNFSKDFLE
HHCCCCCEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCEEEEEEECCCCCCCCHHHHH
KNIKGFNGDVVNANIIKTADNQPFVKPYAIKKIDGVRVAIVGYVVPHIPTWEASTPEHFA
HHCCCCCCCEEECEEEEECCCCCCCCCEEEEECCCEEEEEEEHHHCCCCCCCCCCCHHHC
GLEFLDAEEALKKTLKELKGKYDILIGAFHLGREDEKGGDGVPDLAKKFPQFDIIFAGHE
CCEEECHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCHHHHHCCCEEEEEECCC
HAVYNTKIGKVHTIEPGAYGAYLAKGVVMFDTKTKKKIVTTENLPTKGVPEDEELAKKYE
CEEEECCCCEEEEECCCCCHHHHHCCEEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHH
YVDKKSKEYANEVVGEVTKTFIDRPDFITGEEKITTMPTAALQETPVIELINIVQKYYAK
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCHHHHCCCCHHHHHHHHHHHHHH
ADVSAAALFNFGANLKKGPFKRKDVAYIYKFANTLIGVKITGENLLKYMEWSYQFYNQLQ
CCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHEEEEEECHHHHHHHHHHHHHHHCCCC
PGDLTISFNENIRGYNFDMFSGVKYQVDVTKPAGSRIINPTINNKPIDPKAVYKLAINNY
CCCEEEEECCCCCCCCEEHCCCEEEEEEEECCCCCEEECCCCCCCCCCHHHEEEEEECCE
RFGTLSKTLNLVTDANRYYNSYDGLQDNGQIRDLIIKYITEEKGGKVTPELEHNWEIINY
ECCHHHHHHHHHHCCHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEE
NFKNPLLEKLREKLKEGSIKIPTSKDGRTLNVKSIKESEVK
ECCCHHHHHHHHHHHCCCEECCCCCCCCEEEECCCCCCCCC
>Mature Secondary Structure
MKKLVLIVFLTLTLSISAKEVKIVFLETSDIHGRLFSYDYATGEQKPNNGLTRIAALIKK
CCCEEEEEEEHHHHEEECCEEEEEEEECCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHH
QRAENKNVVLIDSGDLLQGNSAELFNDEPIHPLVLAENDLKFDIRVLGNHEFNFSKDFLE
HHCCCCCEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCEEEEEEECCCCCCCCHHHHH
KNIKGFNGDVVNANIIKTADNQPFVKPYAIKKIDGVRVAIVGYVVPHIPTWEASTPEHFA
HHCCCCCCCEEECEEEEECCCCCCCCCEEEEECCCEEEEEEEHHHCCCCCCCCCCCHHHC
GLEFLDAEEALKKTLKELKGKYDILIGAFHLGREDEKGGDGVPDLAKKFPQFDIIFAGHE
CCEEECHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCHHHHHCCCEEEEEECCC
HAVYNTKIGKVHTIEPGAYGAYLAKGVVMFDTKTKKKIVTTENLPTKGVPEDEELAKKYE
CEEEECCCCEEEEECCCCCHHHHHCCEEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHH
YVDKKSKEYANEVVGEVTKTFIDRPDFITGEEKITTMPTAALQETPVIELINIVQKYYAK
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCHHHHCCCCHHHHHHHHHHHHHH
ADVSAAALFNFGANLKKGPFKRKDVAYIYKFANTLIGVKITGENLLKYMEWSYQFYNQLQ
CCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHEEEEEECHHHHHHHHHHHHHHHCCCC
PGDLTISFNENIRGYNFDMFSGVKYQVDVTKPAGSRIINPTINNKPIDPKAVYKLAINNY
CCCEEEEECCCCCCCCEEHCCCEEEEEEEECCCCCEEECCCCCCCCCCHHHEEEEEECCE
RFGTLSKTLNLVTDANRYYNSYDGLQDNGQIRDLIIKYITEEKGGKVTPELEHNWEIINY
ECCHHHHHHHHHHCCHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEE
NFKNPLLEKLREKLKEGSIKIPTSKDGRTLNVKSIKESEVK
ECCCHHHHHHHHHHHCCCEECCCCCCCCEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]