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The map label for this gene is nolK [H]

Identifier: 188526854

GI number: 188526854

Start: 41108

End: 42040

Strand: Direct

Name: nolK [H]

Synonym: HPSH_00220

Alternate gene names: 188526854

Gene position: 41108-42040 (Clockwise)

Preceding gene: 188526853

Following gene: 188526866

Centisome position: 2.56

GC content: 41.37

Gene sequence:

>933_bases
ATGAATGAGATTATTTTAATCACCGGTGCCTATGGCATGGTGGGGCAGAACACGGCGTTGTATTTTAAAAAAAATAAGCC
TGATGTGGCGTTACTCACCCCTAAAAAGAGCGAATTGTGTTTATTGGATAAAGACAATGTTCAAGCTTATTTGAAAGAAT
ACAAGCCTACAGGCATTATCCATTGCGCCGGGAGAGTGGGGGGCATTGTCGCTAACATGAACGATCTTTCAGCTTACATG
GTTGAGAATTTATTGATGGGCTTGTACCTCTTTTCTAGCGCTTTAGATTTGGGCGTGAAGAAAGCCATTAATTTGGCGAG
CTCTTGCGCTTACCCTAAATTCGCTCCTAACCCTTTAAAAGAGAGCGATTTATTGAACGGATCTTTAGAGCCAACAAACG
AAGGCTACGCATTAGCCAAACTCTCTGTGATGAAGTATTGCGAGTATGTGAGCGCTGAAAAAGGCGTTTTTTATAAAACC
CTAGTGCCTTGCAACCTTTATGGCGAGTTTGACAAGTTTGAAGAAAAAATAGCGCACATGATACCCGGGCTTATTGCTAG
GATGCACACCGCTAAATTAAAAAATGAAAAAGAGTTTGCGATGTGGGGCGATGGCACGGCCAGAAGAGAATATCTAAACG
CTAAAGATTTAGCCAGGTTCATTTCTCTAGCTTATGAGAATATCGATTCAATCCCTAGCGTGATGAATGTCGGCTCTGGC
GTGGATTACAGCATTGAAGAGTATTACAAAATGGTCGCTCAGGTTTTAGATTATAAGGGCGCGTTTGTGAAAGACTTGTC
CAAACCAGTGGGCATGCAGCAAAAGCTTATGGATATTTCCAAACAAAAGGCTTTAAAATGGGAATTAGAAATCCCTTTAG
AGCAGGGCATCAAAGAAGCTTATGAGTATTATTTGAAGCTTTTAGAGGTTTGA

Upstream 100 bases:

>100_bases
GATTTAAAAGAGTTGGTTAAGGACATGTTAGAATACGATTTAAAAGAATGCCAGAAAAACCTTTACTTGCAAGATGGGGG
TTATATTTTAAGGAATTTTT

Downstream 100 bases:

>100_bases
AATAAAATCAAGGCTCTTATAGGGTTATAAAAACGCTCCGCTATTAAGCATTAGGCTAGCGGTAGTTGCGATATTGTGAT
TATTTGGCTTTAAACAAGCT

Product: nodulation protein (nolK)

Products: GDP-4-dehydro-6-deoxy-D-mannose; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MNEIILITGAYGMVGQNTALYFKKNKPDVALLTPKKSELCLLDKDNVQAYLKEYKPTGIIHCAGRVGGIVANMNDLSAYM
VENLLMGLYLFSSALDLGVKKAINLASSCAYPKFAPNPLKESDLLNGSLEPTNEGYALAKLSVMKYCEYVSAEKGVFYKT
LVPCNLYGEFDKFEEKIAHMIPGLIARMHTAKLKNEKEFAMWGDGTARREYLNAKDLARFISLAYENIDSIPSVMNVGSG
VDYSIEEYYKMVAQVLDYKGAFVKDLSKPVGMQQKLMDISKQKALKWELEIPLEQGIKEAYEYYLKLLEV

Sequences:

>Translated_310_residues
MNEIILITGAYGMVGQNTALYFKKNKPDVALLTPKKSELCLLDKDNVQAYLKEYKPTGIIHCAGRVGGIVANMNDLSAYM
VENLLMGLYLFSSALDLGVKKAINLASSCAYPKFAPNPLKESDLLNGSLEPTNEGYALAKLSVMKYCEYVSAEKGVFYKT
LVPCNLYGEFDKFEEKIAHMIPGLIARMHTAKLKNEKEFAMWGDGTARREYLNAKDLARFISLAYENIDSIPSVMNVGSG
VDYSIEEYYKMVAQVLDYKGAFVKDLSKPVGMQQKLMDISKQKALKWELEIPLEQGIKEAYEYYLKLLEV
>Mature_310_residues
MNEIILITGAYGMVGQNTALYFKKNKPDVALLTPKKSELCLLDKDNVQAYLKEYKPTGIIHCAGRVGGIVANMNDLSAYM
VENLLMGLYLFSSALDLGVKKAINLASSCAYPKFAPNPLKESDLLNGSLEPTNEGYALAKLSVMKYCEYVSAEKGVFYKT
LVPCNLYGEFDKFEEKIAHMIPGLIARMHTAKLKNEKEFAMWGDGTARREYLNAKDLARFISLAYENIDSIPSVMNVGSG
VDYSIEEYYKMVAQVLDYKGAFVKDLSKPVGMQQKLMDISKQKALKWELEIPLEQGIKEAYEYYLKLLEV

Specific function: Probable nucleotide sugar epimerase/dehydrogenase [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fucose synthase family [H]

Homologues:

Organism=Homo sapiens, GI4507709, Length=306, Percent_Identity=27.1241830065359, Blast_Score=132, Evalue=3e-31,
Organism=Escherichia coli, GI1788365, Length=310, Percent_Identity=37.4193548387097, Blast_Score=217, Evalue=7e-58,
Organism=Caenorhabditis elegans, GI17554428, Length=307, Percent_Identity=26.0586319218241, Blast_Score=118, Evalue=3e-27,
Organism=Drosophila melanogaster, GI19922778, Length=311, Percent_Identity=27.9742765273312, Blast_Score=145, Evalue=4e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 1.1.1.271

Molecular weight: Translated: 34769; Mature: 34769

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEIILITGAYGMVGQNTALYFKKNKPDVALLTPKKSELCLLDKDNVQAYLKEYKPTGII
CCCEEEEEECCCCCCCCEEEEEECCCCCEEEECCCCCCEEEECCHHHHHHHHHCCCCCEE
HCAGRVGGIVANMNDLSAYMVENLLMGLYLFSSALDLGVKKAINLASSCAYPKFAPNPLK
EECCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
ESDLLNGSLEPTNEGYALAKLSVMKYCEYVSAEKGVFYKTLVPCNLYGEFDKFEEKIAHM
CHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHH
IPGLIARMHTAKLKNEKEFAMWGDGTARREYLNAKDLARFISLAYENIDSIPSVMNVGSG
HHHHHHHHHHHHHCCCCCEEEECCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
VDYSIEEYYKMVAQVLDYKGAFVKDLSKPVGMQQKLMDISKQKALKWELEIPLEQGIKEA
CCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHEEEEEECCHHHHHHHH
YEYYLKLLEV
HHHHHHHHCC
>Mature Secondary Structure
MNEIILITGAYGMVGQNTALYFKKNKPDVALLTPKKSELCLLDKDNVQAYLKEYKPTGII
CCCEEEEEECCCCCCCCEEEEEECCCCCEEEECCCCCCEEEECCHHHHHHHHHCCCCCEE
HCAGRVGGIVANMNDLSAYMVENLLMGLYLFSSALDLGVKKAINLASSCAYPKFAPNPLK
EECCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
ESDLLNGSLEPTNEGYALAKLSVMKYCEYVSAEKGVFYKTLVPCNLYGEFDKFEEKIAHM
CHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHH
IPGLIARMHTAKLKNEKEFAMWGDGTARREYLNAKDLARFISLAYENIDSIPSVMNVGSG
HHHHHHHHHHHHHCCCCCEEEECCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
VDYSIEEYYKMVAQVLDYKGAFVKDLSKPVGMQQKLMDISKQKALKWELEIPLEQGIKEA
CCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHEEEEEECCHHHHHHHH
YEYYLKLLEV
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GDP-L-fucose; NADP+

Specific reaction: GDP-L-fucose + NADP+ = GDP-4-dehydro-6-deoxy-D-mannose + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1472718 [H]