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The map label for this gene is gmd [H]

Identifier: 188526853

GI number: 188526853

Start: 39970

End: 41115

Strand: Direct

Name: gmd [H]

Synonym: HPSH_00215

Alternate gene names: 188526853

Gene position: 39970-41115 (Clockwise)

Preceding gene: 188526852

Following gene: 188526854

Centisome position: 2.48

GC content: 40.75

Gene sequence:

>1146_bases
ATGAAAGAAAAAATCGCTTTAATCACCGGGGTTACCGGGCAAGATGGGAGCTATCTGGCTGAATACTTGCTGAATTTGGG
TTATGAAGTGCATGGGTTAAAAAGGCGCTCTTCTAGCATCAACACTTCTAGGATCGATCATTTGTATGAAGATTTGCACA
GCGAGCACAAAAGGCGTTTTTTCTTGCACTATGGGGATATGACCGATAGCTCTAATCTTATCCATTTAATCGCTACCACT
AAGCCTACAGAGATTTATAATTTAGCCGCTCAAAGCCATGTGAAAGTCTCTTTTGAAACCCCAGAATACACCGCTAACGC
TGATGGTATTGGCACGCTAAGGATTTTAGAAGCCATGCGGATTTTAGGCTTAGAAAAGAAAACACGATTCTATCAAGCCA
GCACGAGCGAATTGTATGGCGAAGTTTTAGAAACCCCACAAAACGAAAACACCCCCTTTAACCCACGAAGCCCCTATGCG
GTCGCTAAAATGTATGCCTTTTACATCACCAAAAATTACAGAGAGGCCTATAACTTGTTTGCGGTTAATGGCATTCTTTT
TAACCATGAGAGCAGGGTAAGGGGCGAAACTTTTGTCACTCGTAAAATCACACGAGCCGCTAGCGCGATAGCGTATAACT
TAACGGATTGCTTGTATTTAGGGAATTTAGACGCTAAAAGAGATTGGGGGCATGCCAAAGATTATGTGAAAATGATGCAT
TTAATGCTCCAAGCGCCCATTCCACAAGATTATGTGATCGCTACAGGAAAGACCACAAGCGTGCGCGATTTTGTGAAAAT
GAGCTTTGAATTTATCGGTATTGGTTTGGAGTTTCAAAATACAGGGATTAAAGAAATCGGTTTGATTAAAAGCGTTGATG
AAAAAAGAGCGAACGCTTTAAGATTAAACTTAAGCCATTTAAAAAAAGGCCAAATCGTGGTGCGCATAGACGAGTGCTAT
TTCAGGCCTACTGAAGTGGATTTGCTTTTAGGCGATCCCACTAAGGCAGAAAAAGAGCTAGGCTGGGTTAGGGAATACGA
TTTAAAAGAGTTGGTTAAGGACATGTTAGAATACGATTTAAAAGAATGCCAGAAAAACCTTTACTTGCAAGATGGGGGTT
ATATTTTAAGGAATTTTTATGAATGA

Upstream 100 bases:

>100_bases
GGGCGAGTATGTCGGTGAAGACGATATTGTAAGGGTTGATGATGATTTTAACAGACAAAATCAAAACGCCTAATAAAGAA
AATTCAATAAAGGAAAAATA

Downstream 100 bases:

>100_bases
GATTATTTTAATCACCGGTGCCTATGGCATGGTGGGGCAGAACACGGCGTTGTATTTTAAAAAAAATAAGCCTGATGTGG
CGTTACTCACCCCTAAAAAG

Product: GDP-D-mannose dehydratase (rfbD)

Products: NA

Alternate protein names: GDP-D-mannose dehydratase; ORF13.7 [H]

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MKEKIALITGVTGQDGSYLAEYLLNLGYEVHGLKRRSSSINTSRIDHLYEDLHSEHKRRFFLHYGDMTDSSNLIHLIATT
KPTEIYNLAAQSHVKVSFETPEYTANADGIGTLRILEAMRILGLEKKTRFYQASTSELYGEVLETPQNENTPFNPRSPYA
VAKMYAFYITKNYREAYNLFAVNGILFNHESRVRGETFVTRKITRAASAIAYNLTDCLYLGNLDAKRDWGHAKDYVKMMH
LMLQAPIPQDYVIATGKTTSVRDFVKMSFEFIGIGLEFQNTGIKEIGLIKSVDEKRANALRLNLSHLKKGQIVVRIDECY
FRPTEVDLLLGDPTKAEKELGWVREYDLKELVKDMLEYDLKECQKNLYLQDGGYILRNFYE

Sequences:

>Translated_381_residues
MKEKIALITGVTGQDGSYLAEYLLNLGYEVHGLKRRSSSINTSRIDHLYEDLHSEHKRRFFLHYGDMTDSSNLIHLIATT
KPTEIYNLAAQSHVKVSFETPEYTANADGIGTLRILEAMRILGLEKKTRFYQASTSELYGEVLETPQNENTPFNPRSPYA
VAKMYAFYITKNYREAYNLFAVNGILFNHESRVRGETFVTRKITRAASAIAYNLTDCLYLGNLDAKRDWGHAKDYVKMMH
LMLQAPIPQDYVIATGKTTSVRDFVKMSFEFIGIGLEFQNTGIKEIGLIKSVDEKRANALRLNLSHLKKGQIVVRIDECY
FRPTEVDLLLGDPTKAEKELGWVREYDLKELVKDMLEYDLKECQKNLYLQDGGYILRNFYE
>Mature_381_residues
MKEKIALITGVTGQDGSYLAEYLLNLGYEVHGLKRRSSSINTSRIDHLYEDLHSEHKRRFFLHYGDMTDSSNLIHLIATT
KPTEIYNLAAQSHVKVSFETPEYTANADGIGTLRILEAMRILGLEKKTRFYQASTSELYGEVLETPQNENTPFNPRSPYA
VAKMYAFYITKNYREAYNLFAVNGILFNHESRVRGETFVTRKITRAASAIAYNLTDCLYLGNLDAKRDWGHAKDYVKMMH
LMLQAPIPQDYVIATGKTTSVRDFVKMSFEFIGIGLEFQNTGIKEIGLIKSVDEKRANALRLNLSHLKKGQIVVRIDECY
FRPTEVDLLLGDPTKAEKELGWVREYDLKELVKDMLEYDLKECQKNLYLQDGGYILRNFYE

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG1089

COG function: function code M; GDP-D-mannose dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=362, Percent_Identity=54.9723756906077, Blast_Score=402, Evalue=1e-112,
Organism=Escherichia coli, GI1788366, Length=371, Percent_Identity=60.1078167115903, Blast_Score=465, Evalue=1e-132,
Organism=Escherichia coli, GI48994969, Length=379, Percent_Identity=22.9551451187335, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1788353, Length=257, Percent_Identity=23.7354085603113, Blast_Score=66, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI133901788, Length=358, Percent_Identity=56.7039106145251, Blast_Score=408, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI17539422, Length=358, Percent_Identity=56.7039106145251, Blast_Score=408, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI17539424, Length=358, Percent_Identity=56.7039106145251, Blast_Score=408, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI133901786, Length=358, Percent_Identity=56.7039106145251, Blast_Score=407, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI133901790, Length=358, Percent_Identity=56.7039106145251, Blast_Score=407, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI17507723, Length=358, Percent_Identity=57.2625698324022, Blast_Score=403, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24158427, Length=365, Percent_Identity=57.8082191780822, Blast_Score=432, Evalue=1e-121,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 43800; Mature: 43800

Theoretical pI: Translated: 7.37; Mature: 7.37

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEKIALITGVTGQDGSYLAEYLLNLGYEVHGLKRRSSSINTSRIDHLYEDLHSEHKRRF
CCCCEEEEEECCCCCHHHHHHHHHHCCCEEECHHHHCCCCCHHHHHHHHHHHHHHHCEEE
FLHYGDMTDSSNLIHLIATTKPTEIYNLAAQSHVKVSFETPEYTANADGIGTLRILEAMR
EEEECCCCCCCCEEEEEEECCCCHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHHHH
ILGLEKKTRFYQASTSELYGEVLETPQNENTPFNPRSPYAVAKMYAFYITKNYREAYNLF
HHCCCHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEE
AVNGILFNHESRVRGETFVTRKITRAASAIAYNLTDCLYLGNLDAKRDWGHAKDYVKMMH
EEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCHHHHHHHHHH
LMLQAPIPQDYVIATGKTTSVRDFVKMSFEFIGIGLEFQNTGIKEIGLIKSVDEKRANAL
HHHHCCCCCCEEEECCCCCHHHHHHHHCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHEE
RLNLSHLKKGQIVVRIDECYFRPTEVDLLLGDPTKAEKELGWVREYDLKELVKDMLEYDL
EEEHHHCCCCCEEEEEHHHCCCCCCEEEEECCCCCHHHHHCCHHHCCHHHHHHHHHHHHH
KECQKNLYLQDGGYILRNFYE
HHHHHCCEECCCCHHHHCCCC
>Mature Secondary Structure
MKEKIALITGVTGQDGSYLAEYLLNLGYEVHGLKRRSSSINTSRIDHLYEDLHSEHKRRF
CCCCEEEEEECCCCCHHHHHHHHHHCCCEEECHHHHCCCCCHHHHHHHHHHHHHHHCEEE
FLHYGDMTDSSNLIHLIATTKPTEIYNLAAQSHVKVSFETPEYTANADGIGTLRILEAMR
EEEECCCCCCCCEEEEEEECCCCHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHHHH
ILGLEKKTRFYQASTSELYGEVLETPQNENTPFNPRSPYAVAKMYAFYITKNYREAYNLF
HHCCCHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEE
AVNGILFNHESRVRGETFVTRKITRAASAIAYNLTDCLYLGNLDAKRDWGHAKDYVKMMH
EEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCHHHHHHHHHH
LMLQAPIPQDYVIATGKTTSVRDFVKMSFEFIGIGLEFQNTGIKEIGLIKSVDEKRANAL
HHHHCCCCCCEEEECCCCCHHHHHHHHCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHEE
RLNLSHLKKGQIVVRIDECYFRPTEVDLLLGDPTKAEKELGWVREYDLKELVKDMLEYDL
EEEHHHCCCCCEEEEEHHHCCCCCCEEEEECCCCCHHHHHCCHHHCCHHHHHHHHHHHHH
KECQKNLYLQDGGYILRNFYE
HHHHHCCEECCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8932701 [H]