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The map label for this gene is dnaG

Identifier: 188526822

GI number: 188526822

Start: 9663

End: 11342

Strand: Direct

Name: dnaG

Synonym: HPSH_00060

Alternate gene names: 188526822

Gene position: 9663-11342 (Clockwise)

Preceding gene: 188526818

Following gene: 188526823

Centisome position: 0.6

GC content: 39.4

Gene sequence:

>1680_bases
ATGATTCTTAAAAGTTCCATTGATCGCCTTTTGCAAACGATAGATATTGTAGAAGTCATTAGCTCCTATGTGGATTTGAG
GAAATCAGGTTCTAGTTACATGGCTTGTTGCCCTTTTCATGAAGAAAGGAGTGCGAGTTTTAGCGTCAATCAAATTAAAG
GGTTTTACCATTGCTTTGGGTGTGGGGCGAGCGGGGATAGCATTAAATTTGTGATGGAGTTTGAAAAGCTTTCGTTTGTG
GAAGCATTAGAGAAATTAGCCCATCGATTCAATGTGGCTTTAGAGTATGACAAAGGCGTTTATTACGATCATAAAGAAGA
CTACCACCTTTTAGAAATGGTGAGCTCGTTGTATCAAGAAGAGCTTTTTAACGCCCCGTTTTTTTTGAATTATTTGCAAA
AAAGAGGGCTTAGCATAGAGAGTATAAGAGCGTTTAAATTAGGCTTATGCACGAATAGAATTGATTACGACATTGAAAAT
AAAGGCTTGAATAAGGACAAGCTCATTGAATTAGGCGTGTTGGGCAAGAGCGATAAAAAGGATAGAACCTATTTGCGCTT
TTTGGATCGCATCATGTTCCCTATTTATAGCCCTAGCGCTCAAGTGGTGGGCTTTGGAGGGCGCACCTTAAAAGAAAAAG
CGGCCAAGTATATCAATTCGCCCCAAAATAAGCTTTTTGATAAATCCAGTTTGCTCTATGGCTACCATTTGGCTAAAGAA
CACATCTATAAACAAAAGCAAGTCATTGTAACAGAGGGGTATTTGGATGTGATTTTATTGCACCAGGCGGGTTTTAAAAA
CGCCATAGCCACGCTTGGGACAGCTTTAACGCCATCGCATTTGCCCTTGCTTAAAAAAGGCGAGCCAGAAATTCTTTTGA
GCTATGATGGGGATAATGCAGGGCGAAACGCGGCTTATAAAGCGAGCTTGATGTTGGCTAAAGAGCAAAGGAGGGGAGGG
GTGATTCTATTTGAAAACAACCTGGATCCAGCGGATATGATCGCTAATGGCCAGATTGAAACCTTAAAAAATTGGCTATC
GCGCCCCATGGCTTTTATTGAATTTGTTTTAAGGCACATGGCGGGTTCTTATCTTTTAGACGATCCTTTAGAAAAAGATA
AGGCCCTTAAAGAAATGTTAGGATTTTTAAAAAACTTTTCCTTGCTTTTGCAAAGCGAATACAAGCCCTTAATCGCTTCT
CTTTTGCAAGTGCCTTTGCATGTTTTAGGGATTAGAGAGCGAGCCTCTTTTCAGCCTTTTTACCCCAAAACAGAAAAACC
TAATCATACTCAAAAGTTGGTTAATGCTTCTAGCACGCTCAGTTTGGAATTTTTAGAAAAATTAGTGATCCGCTATCTTT
TAGAAGACAGAAGCTTGTTGGATTTGGCGGTGGGTTATATCCATAGTGGGGTATTCTTGCATAAAAAACAAGAATTTGAC
GCTTTGTGTCAAGAAAAATTGGACGACCCTAAATTAGTTGCGTTATTATTAGATGCGAATTTACCCCTAAAAAAAGGGGG
TTTTGAAAAGGAATTGCGTTTGTTGATTTTGCGCTATTTTGAGCGGCAACTCAAAGAAATCCCTAAAAGCTCGCTCCCTT
TTAGCGAAAAAATGATCTTTTTAAAAAAGGCTCGCCAGGCCATTATGAAATTAAAACAAGGAGAATTAGTCGCCATATGA

Upstream 100 bases:

>100_bases
AGGGGGCGTTATCAATAAACCTTTTTTAAGATTTTATTTATTGAGTATAATTCATTCAAGCGAGTTACAACAAGAAAGCA
AGATGAAGAAAGCATGAAAA

Downstream 100 bases:

>100_bases
AACCAATAAAAGCTTTAGCCTTATTTAGTGGGGGGTTGGATAGTTTGTTGTCTATGAAATTACTCATTGATCAAGGCATT
GAAGTGAGTGCTTTACACTT

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 559; Mature: 559

Protein sequence:

>559_residues
MILKSSIDRLLQTIDIVEVISSYVDLRKSGSSYMACCPFHEERSASFSVNQIKGFYHCFGCGASGDSIKFVMEFEKLSFV
EALEKLAHRFNVALEYDKGVYYDHKEDYHLLEMVSSLYQEELFNAPFFLNYLQKRGLSIESIRAFKLGLCTNRIDYDIEN
KGLNKDKLIELGVLGKSDKKDRTYLRFLDRIMFPIYSPSAQVVGFGGRTLKEKAAKYINSPQNKLFDKSSLLYGYHLAKE
HIYKQKQVIVTEGYLDVILLHQAGFKNAIATLGTALTPSHLPLLKKGEPEILLSYDGDNAGRNAAYKASLMLAKEQRRGG
VILFENNLDPADMIANGQIETLKNWLSRPMAFIEFVLRHMAGSYLLDDPLEKDKALKEMLGFLKNFSLLLQSEYKPLIAS
LLQVPLHVLGIRERASFQPFYPKTEKPNHTQKLVNASSTLSLEFLEKLVIRYLLEDRSLLDLAVGYIHSGVFLHKKQEFD
ALCQEKLDDPKLVALLLDANLPLKKGGFEKELRLLILRYFERQLKEIPKSSLPFSEKMIFLKKARQAIMKLKQGELVAI

Sequences:

>Translated_559_residues
MILKSSIDRLLQTIDIVEVISSYVDLRKSGSSYMACCPFHEERSASFSVNQIKGFYHCFGCGASGDSIKFVMEFEKLSFV
EALEKLAHRFNVALEYDKGVYYDHKEDYHLLEMVSSLYQEELFNAPFFLNYLQKRGLSIESIRAFKLGLCTNRIDYDIEN
KGLNKDKLIELGVLGKSDKKDRTYLRFLDRIMFPIYSPSAQVVGFGGRTLKEKAAKYINSPQNKLFDKSSLLYGYHLAKE
HIYKQKQVIVTEGYLDVILLHQAGFKNAIATLGTALTPSHLPLLKKGEPEILLSYDGDNAGRNAAYKASLMLAKEQRRGG
VILFENNLDPADMIANGQIETLKNWLSRPMAFIEFVLRHMAGSYLLDDPLEKDKALKEMLGFLKNFSLLLQSEYKPLIAS
LLQVPLHVLGIRERASFQPFYPKTEKPNHTQKLVNASSTLSLEFLEKLVIRYLLEDRSLLDLAVGYIHSGVFLHKKQEFD
ALCQEKLDDPKLVALLLDANLPLKKGGFEKELRLLILRYFERQLKEIPKSSLPFSEKMIFLKKARQAIMKLKQGELVAI
>Mature_559_residues
MILKSSIDRLLQTIDIVEVISSYVDLRKSGSSYMACCPFHEERSASFSVNQIKGFYHCFGCGASGDSIKFVMEFEKLSFV
EALEKLAHRFNVALEYDKGVYYDHKEDYHLLEMVSSLYQEELFNAPFFLNYLQKRGLSIESIRAFKLGLCTNRIDYDIEN
KGLNKDKLIELGVLGKSDKKDRTYLRFLDRIMFPIYSPSAQVVGFGGRTLKEKAAKYINSPQNKLFDKSSLLYGYHLAKE
HIYKQKQVIVTEGYLDVILLHQAGFKNAIATLGTALTPSHLPLLKKGEPEILLSYDGDNAGRNAAYKASLMLAKEQRRGG
VILFENNLDPADMIANGQIETLKNWLSRPMAFIEFVLRHMAGSYLLDDPLEKDKALKEMLGFLKNFSLLLQSEYKPLIAS
LLQVPLHVLGIRERASFQPFYPKTEKPNHTQKLVNASSTLSLEFLEKLVIRYLLEDRSLLDLAVGYIHSGVFLHKKQEFD
ALCQEKLDDPKLVALLLDANLPLKKGGFEKELRLLILRYFERQLKEIPKSSLPFSEKMIFLKKARQAIMKLKQGELVAI

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=377, Percent_Identity=35.8090185676393, Blast_Score=246, Evalue=3e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013264
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 63814; Mature: 63814

Theoretical pI: Translated: 9.12; Mature: 9.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILKSSIDRLLQTIDIVEVISSYVDLRKSGSSYMACCPFHEERSASFSVNQIKGFYHCFG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHEEE
CGASGDSIKFVMEFEKLSFVEALEKLAHRFNVALEYDKGVYYDHKEDYHLLEMVSSLYQE
CCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCHHHHHHHHHHHHH
ELFNAPFFLNYLQKRGLSIESIRAFKLGLCTNRIDYDIENKGLNKDKLIELGVLGKSDKK
HHCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHEEEEECCCCCCCC
DRTYLRFLDRIMFPIYSPSAQVVGFGGRTLKEKAAKYINSPQNKLFDKSSLLYGYHLAKE
HHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHH
HIYKQKQVIVTEGYLDVILLHQAGFKNAIATLGTALTPSHLPLLKKGEPEILLSYDGDNA
HHHHHCCEEEECCCHHHHEEHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCC
GRNAAYKASLMLAKEQRRGGVILFENNLDPADMIANGQIETLKNWLSRPMAFIEFVLRHM
CCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
AGSYLLDDPLEKDKALKEMLGFLKNFSLLLQSEYKPLIASLLQVPLHVLGIRERASFQPF
CCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHCCCCCC
YPKTEKPNHTQKLVNASSTLSLEFLEKLVIRYLLEDRSLLDLAVGYIHSGVFLHKKQEFD
CCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEECHHHHH
ALCQEKLDDPKLVALLLDANLPLKKGGFEKELRLLILRYFERQLKEIPKSSLPFSEKMIF
HHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
LKKARQAIMKLKQGELVAI
HHHHHHHHHHHCCCCEEEC
>Mature Secondary Structure
MILKSSIDRLLQTIDIVEVISSYVDLRKSGSSYMACCPFHEERSASFSVNQIKGFYHCFG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHEEE
CGASGDSIKFVMEFEKLSFVEALEKLAHRFNVALEYDKGVYYDHKEDYHLLEMVSSLYQE
CCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCHHHHHHHHHHHHH
ELFNAPFFLNYLQKRGLSIESIRAFKLGLCTNRIDYDIENKGLNKDKLIELGVLGKSDKK
HHCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHEEEEECCCCCCCC
DRTYLRFLDRIMFPIYSPSAQVVGFGGRTLKEKAAKYINSPQNKLFDKSSLLYGYHLAKE
HHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHH
HIYKQKQVIVTEGYLDVILLHQAGFKNAIATLGTALTPSHLPLLKKGEPEILLSYDGDNA
HHHHHCCEEEECCCHHHHEEHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCC
GRNAAYKASLMLAKEQRRGGVILFENNLDPADMIANGQIETLKNWLSRPMAFIEFVLRHM
CCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
AGSYLLDDPLEKDKALKEMLGFLKNFSLLLQSEYKPLIASLLQVPLHVLGIRERASFQPF
CCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHCCCCCC
YPKTEKPNHTQKLVNASSTLSLEFLEKLVIRYLLEDRSLLDLAVGYIHSGVFLHKKQEFD
CCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEECHHHHH
ALCQEKLDDPKLVALLLDANLPLKKGGFEKELRLLILRYFERQLKEIPKSSLPFSEKMIF
HHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
LKKARQAIMKLKQGELVAI
HHHHHHHHHHHCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]