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The map label for this gene is glpF [H]

Identifier: 187935597

GI number: 187935597

Start: 2193383

End: 2194093

Strand: Reverse

Name: glpF [H]

Synonym: CLL_A2097

Alternate gene names: 187935597

Gene position: 2194093-2193383 (Counterclockwise)

Preceding gene: 187934841

Following gene: 187935241

Centisome position: 57.73

GC content: 34.74

Gene sequence:

>711_bases
ATGAATATGACAACATTTTTTGCAGAAATGTTTGGAACTTTAATCTTAGTATTATTAGGAGATGCTGTTGTAGCTAACGT
AATACTTAAGAAAACAAAGGGTCAAAATTCAGGATGGATGGTTATATCAAGTGGATGGGCTGTAGCTGTTGCAATTCCAG
TATATATGTTTGCAAATATTAGTGGAGCACATTTTAACCCTGCAGTTACAATAGCATTAGCTACTGTTGGACAATTTCCA
TGGGCTGATGTTCCTATGTATATAACTGCACAATTTATAGGAGCATTTATAGGAGCTACTTTAGTTCTTATTTCTTACTA
TAATCATTTTGAAGCAACTGAAGATCAAGCTACAAAATTAGGAGTTTTTTGTACAGCTCCAGAAATAAGAAAACCTGTAT
TTAACTTTATAACTGAGTTTGTGGGAACATTTATATTAGTTTTTGCAATAATGGGAGCAACAGCACAACCATTTGTAAAT
GGAACTCAACCAGTAGCTATAGGTGTAATAATATTAGCAATGGGATTATGTTTAGGTGGTCCAACAGGATATGCGATAAA
CCCAGCTAGAGATTTAGCACCAAGACTTGTTCACTTTTTATTGCCAGTACCAAATAAAGGTACTTCAGATTGGGGCTATG
CATGGATACCAGTAGTAGCACCAATAGCAGGAGCAATAGCAGGAGCTTTAGTTTACGTAAATATATTCTAA

Upstream 100 bases:

>100_bases
TATTAGAACCCCTTTACATTTAAAGGGGTTCTTTAAATGAAATTATATAAATTCATAAGATAAATTAATAAGAAACATAA
GATTTTAGGAGGGCTAATAT

Downstream 100 bases:

>100_bases
GATTAGTTTTAGTAGAGTGTTGATGTATGCATAAGTAAAGTGAACTGAGTAATTGAACTTCACTTATTGCATATATCAAC
ACATATTTAAAATAGAGCTT

Product: glycerol uptake facilitator protein

Products: glycerol [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MNMTTFFAEMFGTLILVLLGDAVVANVILKKTKGQNSGWMVISSGWAVAVAIPVYMFANISGAHFNPAVTIALATVGQFP
WADVPMYITAQFIGAFIGATLVLISYYNHFEATEDQATKLGVFCTAPEIRKPVFNFITEFVGTFILVFAIMGATAQPFVN
GTQPVAIGVIILAMGLCLGGPTGYAINPARDLAPRLVHFLLPVPNKGTSDWGYAWIPVVAPIAGAIAGALVYVNIF

Sequences:

>Translated_236_residues
MNMTTFFAEMFGTLILVLLGDAVVANVILKKTKGQNSGWMVISSGWAVAVAIPVYMFANISGAHFNPAVTIALATVGQFP
WADVPMYITAQFIGAFIGATLVLISYYNHFEATEDQATKLGVFCTAPEIRKPVFNFITEFVGTFILVFAIMGATAQPFVN
GTQPVAIGVIILAMGLCLGGPTGYAINPARDLAPRLVHFLLPVPNKGTSDWGYAWIPVVAPIAGAIAGALVYVNIF
>Mature_236_residues
MNMTTFFAEMFGTLILVLLGDAVVANVILKKTKGQNSGWMVISSGWAVAVAIPVYMFANISGAHFNPAVTIALATVGQFP
WADVPMYITAQFIGAFIGATLVLISYYNHFEATEDQATKLGVFCTAPEIRKPVFNFITEFVGTFILVFAIMGATAQPFVN
GTQPVAIGVIILAMGLCLGGPTGYAINPARDLAPRLVHFLLPVPNKGTSDWGYAWIPVVAPIAGAIAGALVYVNIF

Specific function: Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI157266307, Length=243, Percent_Identity=37.8600823045267, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI4826645, Length=246, Percent_Identity=35.3658536585366, Blast_Score=136, Evalue=2e-32,
Organism=Homo sapiens, GI4502187, Length=243, Percent_Identity=34.156378600823, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI22538420, Length=239, Percent_Identity=33.4728033472803, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI310133356, Length=219, Percent_Identity=30.1369863013699, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI310114181, Length=219, Percent_Identity=30.1369863013699, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI1790362, Length=248, Percent_Identity=34.6774193548387, Blast_Score=107, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI71994009, Length=237, Percent_Identity=35.0210970464135, Blast_Score=129, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17544068, Length=244, Percent_Identity=34.8360655737705, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI32564052, Length=236, Percent_Identity=30.5084745762712, Blast_Score=103, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17533613, Length=236, Percent_Identity=30.5084745762712, Blast_Score=103, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17531431, Length=235, Percent_Identity=31.4893617021277, Blast_Score=101, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17531429, Length=235, Percent_Identity=31.4893617021277, Blast_Score=100, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI71992966, Length=241, Percent_Identity=32.3651452282158, Blast_Score=100, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI71992961, Length=239, Percent_Identity=29.7071129707113, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17558372, Length=233, Percent_Identity=30.0429184549356, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71993722, Length=182, Percent_Identity=30.7692307692308, Blast_Score=67, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6321054, Length=204, Percent_Identity=37.7450980392157, Blast_Score=114, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6322985, Length=283, Percent_Identity=29.6819787985866, Blast_Score=87, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24652747, Length=182, Percent_Identity=29.6703296703297, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI45551084, Length=182, Percent_Identity=29.6703296703297, Blast_Score=74, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 25123; Mature: 25123

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: PS00211 ABC_TRANSPORTER_1 ; PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMTTFFAEMFGTLILVLLGDAVVANVILKKTKGQNSGWMVISSGWAVAVAIPVYMFANI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEHHHHHHHHHCC
SGAHFNPAVTIALATVGQFPWADVPMYITAQFIGAFIGATLVLISYYNHFEATEDQATKL
CCCCCCHHHEEEEEHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHEE
GVFCTAPEIRKPVFNFITEFVGTFILVFAIMGATAQPFVNGTQPVAIGVIILAMGLCLGG
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
PTGYAINPARDLAPRLVHFLLPVPNKGTSDWGYAWIPVVAPIAGAIAGALVYVNIF
CCCCEECCHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHHHHHHEEEEEC
>Mature Secondary Structure
MNMTTFFAEMFGTLILVLLGDAVVANVILKKTKGQNSGWMVISSGWAVAVAIPVYMFANI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEHHHHHHHHHCC
SGAHFNPAVTIALATVGQFPWADVPMYITAQFIGAFIGATLVLISYYNHFEATEDQATKL
CCCCCCHHHEEEEEHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHEE
GVFCTAPEIRKPVFNFITEFVGTFILVFAIMGATAQPFVNGTQPVAIGVIILAMGLCLGG
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
PTGYAINPARDLAPRLVHFLLPVPNKGTSDWGYAWIPVVAPIAGAIAGALVYVNIF
CCCCEECCHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHHHHHHEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]