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The map label for this gene is nth [H]

Identifier: 187935458

GI number: 187935458

Start: 3182601

End: 3183227

Strand: Reverse

Name: nth [H]

Synonym: CLL_A3076

Alternate gene names: 187935458

Gene position: 3183227-3182601 (Counterclockwise)

Preceding gene: 187932810

Following gene: 187934805

Centisome position: 83.76

GC content: 28.71

Gene sequence:

>627_bases
ATGAAAGTAAGGACACAAAAGATTTTAGATATTCTAAAAGAAACTTACCCGGATGCTAAATGTGAATTAAATTATGAAAC
TTCTTTTCAGTTGTTAGTAGCTACAATTTTATCAGCGCAGACCACTGATAAAAAGGTTAATGAGATTACAAAAACTTTAT
TTGAGGATTATCCAGATTTAGATGCTTTTTTAAAGATAACAAATGAAGAATTAGAAGACAGAATAAAACAAATAGGGTTA
TATCGTAACAAATCTAAAAATTTAATACTTATGTTTAGGCAGTTAAAAGAGAATTTTAATGGTGAAGTTCCAGGAACTAT
GGAAGGAATAACTTCTCTTTCAGGAGCAGGTAGAAAAACTGCAAATGTAGTTTTATCAAATGCCTTTGGTGTGCCTTCAA
TAGCTGTAGACACTCATGTTTTTAGAGTATCAAATAGATTAGAACTTGCAAATTCTGAAAATGTATTAGAAGTTGAAATG
CAGTTACAAAAAGAGTTACCTAAAAGTGAGTGGTCGTTAACTCATCATTTGTTAATATTTCATGGAAGAAGATGTTGCAA
ATCTAGGAATCCTAAATGTAAAGAATGTCCATTAAATAATATATGTAAATATGATAATATTAAATAA

Upstream 100 bases:

>100_bases
ATATGATGATAGATGTCTCCATATATTAATGAAGAGTATAGCAAACTTATTACCTGAAGAATACAGGGGAGTTTACAAAT
AAGAGGAGATTATAAACAAT

Downstream 100 bases:

>100_bases
AAAGATAGTTAGAACCACTAGTAATTTAAAGGTTATTATTAGTGGTTCTTTAATTTTTGCAAAAAATTTATTAATATATA
ATTATAAGATATATTAAAAT

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 208; Mature: 208

Protein sequence:

>208_residues
MKVRTQKILDILKETYPDAKCELNYETSFQLLVATILSAQTTDKKVNEITKTLFEDYPDLDAFLKITNEELEDRIKQIGL
YRNKSKNLILMFRQLKENFNGEVPGTMEGITSLSGAGRKTANVVLSNAFGVPSIAVDTHVFRVSNRLELANSENVLEVEM
QLQKELPKSEWSLTHHLLIFHGRRCCKSRNPKCKECPLNNICKYDNIK

Sequences:

>Translated_208_residues
MKVRTQKILDILKETYPDAKCELNYETSFQLLVATILSAQTTDKKVNEITKTLFEDYPDLDAFLKITNEELEDRIKQIGL
YRNKSKNLILMFRQLKENFNGEVPGTMEGITSLSGAGRKTANVVLSNAFGVPSIAVDTHVFRVSNRLELANSENVLEVEM
QLQKELPKSEWSLTHHLLIFHGRRCCKSRNPKCKECPLNNICKYDNIK
>Mature_208_residues
MKVRTQKILDILKETYPDAKCELNYETSFQLLVATILSAQTTDKKVNEITKTLFEDYPDLDAFLKITNEELEDRIKQIGL
YRNKSKNLILMFRQLKENFNGEVPGTMEGITSLSGAGRKTANVVLSNAFGVPSIAVDTHVFRVSNRLELANSENVLEVEM
QLQKELPKSEWSLTHHLLIFHGRRCCKSRNPKCKECPLNNICKYDNIK

Specific function: Has Both An Apurinic And/Or Apyrimidinic Endonuclease Activity And A DNA N-Glycosylase Activity. Incises Damaged DNA At Cytosines, Thymines And Guanines. Acts On A Damaged Strand, 5' From The Damaged Site. Required For The Repair Of Both Oxidative DNA Da

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=177, Percent_Identity=28.8135593220339, Blast_Score=94, Evalue=1e-19,
Organism=Escherichia coli, GI1787920, Length=203, Percent_Identity=44.8275862068966, Blast_Score=174, Evalue=4e-45,
Organism=Caenorhabditis elegans, GI17554540, Length=177, Percent_Identity=35.5932203389831, Blast_Score=113, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6324530, Length=193, Percent_Identity=29.0155440414508, Blast_Score=86, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6319304, Length=179, Percent_Identity=29.0502793296089, Blast_Score=77, Evalue=2e-15,
Organism=Drosophila melanogaster, GI45550361, Length=177, Percent_Identity=31.0734463276836, Blast_Score=89, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 23824; Mature: 23824

Theoretical pI: Translated: 8.36; Mature: 8.36

Prosite motif: PS00764 ENDONUCLEASE_III_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVRTQKILDILKETYPDAKCELNYETSFQLLVATILSAQTTDKKVNEITKTLFEDYPDL
CCCCHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCH
DAFLKITNEELEDRIKQIGLYRNKSKNLILMFRQLKENFNGEVPGTMEGITSLSGAGRKT
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCHH
ANVVLSNAFGVPSIAVDTHVFRVSNRLELANSENVLEVEMQLQKELPKSEWSLTHHLLIF
HHHHEECCCCCCCHHHHHHHHHHHCHHEECCCCCEEHHHHHHHHHCCCCHHHHHHHHHHH
HGRRCCKSRNPKCKECPLNNICKYDNIK
HHHHHHHCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKVRTQKILDILKETYPDAKCELNYETSFQLLVATILSAQTTDKKVNEITKTLFEDYPDL
CCCCHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCH
DAFLKITNEELEDRIKQIGLYRNKSKNLILMFRQLKENFNGEVPGTMEGITSLSGAGRKT
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCHH
ANVVLSNAFGVPSIAVDTHVFRVSNRLELANSENVLEVEMQLQKELPKSEWSLTHHLLIF
HHHHEECCCCCCCHHHHHHHHHHHCHHEECCCCCEEHHHHHHHHHCCCCHHHHHHHHHHH
HGRRCCKSRNPKCKECPLNNICKYDNIK
HHHHHHHCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7704260; 8760912; 9384377 [H]