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The map label for this gene is oppB [H]

Identifier: 187934323

GI number: 187934323

Start: 2584538

End: 2585464

Strand: Reverse

Name: oppB [H]

Synonym: CLL_A2492

Alternate gene names: 187934323

Gene position: 2585464-2584538 (Counterclockwise)

Preceding gene: 187932806

Following gene: 187935328

Centisome position: 68.03

GC content: 30.96

Gene sequence:

>927_bases
ATGCTAAAATACATTGGAAAAAGATTATTAGCAAGTATATTAACAATATGGGTTGTCATAACTTTAACATTCTTCCTTAT
GAGACTTATGCCAGGTGGGCCTTTCGATAGTGAAAAATTAACACCTGAAATTAAAGCTAATATGGAAGCAAAATATGGAA
TGGACAAACCATTAGGAGAGCAATATGCATTGTATATGAAAAACCTAGTTAAAGGGGATCTTGGTGAGTCAATGGTATTC
AAAGGAAGAAGAGTATCAGAAACAATATCATATTCATTCCCAGCATCTGCAAAAATTGGTTTAGTGAGTGTGGCTTCATC
AGTTCTTGTAGGAGTAGGATTAGGGATAGTAGCAGCATTAAAAAAAGGTACATGGGCAGATTCACTTATTATGCTCTTAG
TTACTTTAGGAATTACTATACCAAGTTTTGTAATAGGAGCTGTATTAATTTATTTCTTAACTGTTGAGCTTAGATTATTA
CCAGCAACAGGATTTAGTAGTTGGAAGCACTATATAATGCCAGTAATAGCATTATCACTAGGATCAATGGCTTTTATTAC
AAGACTTACAAGAAACAAATTATTGGATGTGTTAAAATCTGATTATATAAGAACAGCAAAAGCTAAAGGACTAAGTAAAA
GAAAAGTTATATTAAAACATGCACTTAGAAATTCATTAATACCAATAGTAACTTATGTTGGACCATTAATAGCAAGTATT
TTAACTGGTAGTTTCGTTGTAGAAAAAATATTTGCAATTCCTGGATTGGGTAATGAATTTGTTCAATCAGTTACAAATAG
AGATTACACTATGTTACTTGGAGTTACAGTATTTTTCTGTACTTTACTTATAATGTTCAACTTCATAGTTGATATTATGT
ATGTAGTAATTGATCCAAGAATAAAATTAGAGGATGCAGAAGTTTAG

Upstream 100 bases:

>100_bases
TGGTTCTAATTTATTGACAAAAATTAATATTATTATATAATTATTAAATAAATTGATATCATTAGTATATATCGAATTTT
GTAAAAAGGGAGAGGGAAAA

Downstream 100 bases:

>100_bases
TAAAGGGGAGAAAAAAGCATGGAACAAGAAATAAATTTAAAAAAGGACTTATTTACTCCTCTGACTGAAGATGAAAAAAA
GATAGAGGTTATTGTAAGAC

Product: oligopeptide transport system permease protein OppB

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: Stage 0 sporulation protein KB [H]

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MLKYIGKRLLASILTIWVVITLTFFLMRLMPGGPFDSEKLTPEIKANMEAKYGMDKPLGEQYALYMKNLVKGDLGESMVF
KGRRVSETISYSFPASAKIGLVSVASSVLVGVGLGIVAALKKGTWADSLIMLLVTLGITIPSFVIGAVLIYFLTVELRLL
PATGFSSWKHYIMPVIALSLGSMAFITRLTRNKLLDVLKSDYIRTAKAKGLSKRKVILKHALRNSLIPIVTYVGPLIASI
LTGSFVVEKIFAIPGLGNEFVQSVTNRDYTMLLGVTVFFCTLLIMFNFIVDIMYVVIDPRIKLEDAEV

Sequences:

>Translated_308_residues
MLKYIGKRLLASILTIWVVITLTFFLMRLMPGGPFDSEKLTPEIKANMEAKYGMDKPLGEQYALYMKNLVKGDLGESMVF
KGRRVSETISYSFPASAKIGLVSVASSVLVGVGLGIVAALKKGTWADSLIMLLVTLGITIPSFVIGAVLIYFLTVELRLL
PATGFSSWKHYIMPVIALSLGSMAFITRLTRNKLLDVLKSDYIRTAKAKGLSKRKVILKHALRNSLIPIVTYVGPLIASI
LTGSFVVEKIFAIPGLGNEFVQSVTNRDYTMLLGVTVFFCTLLIMFNFIVDIMYVVIDPRIKLEDAEV
>Mature_308_residues
MLKYIGKRLLASILTIWVVITLTFFLMRLMPGGPFDSEKLTPEIKANMEAKYGMDKPLGEQYALYMKNLVKGDLGESMVF
KGRRVSETISYSFPASAKIGLVSVASSVLVGVGLGIVAALKKGTWADSLIMLLVTLGITIPSFVIGAVLIYFLTVELRLL
PATGFSSWKHYIMPVIALSLGSMAFITRLTRNKLLDVLKSDYIRTAKAKGLSKRKVILKHALRNSLIPIVTYVGPLIASI
LTGSFVVEKIFAIPGLGNEFVQSVTNRDYTMLLGVTVFFCTLLIMFNFIVDIMYVVIDPRIKLEDAEV

Specific function: Part of the binding-protein-dependent transport system for oligopeptides; probably responsible for the translocation of the substrate across the membrane. Also required for sporulation and competence [H]

COG id: COG0601

COG function: function code EP; ABC-type dipeptide/oligopeptide/nickel transport systems, permease components

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787497, Length=305, Percent_Identity=44.5901639344262, Blast_Score=280, Evalue=9e-77,
Organism=Escherichia coli, GI1787053, Length=308, Percent_Identity=39.9350649350649, Blast_Score=227, Evalue=6e-61,
Organism=Escherichia coli, GI1789965, Length=338, Percent_Identity=34.0236686390533, Blast_Score=193, Evalue=1e-50,
Organism=Escherichia coli, GI1789888, Length=311, Percent_Identity=29.903536977492, Blast_Score=169, Evalue=2e-43,
Organism=Escherichia coli, GI1788504, Length=363, Percent_Identity=30.3030303030303, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1787761, Length=333, Percent_Identity=29.7297297297297, Blast_Score=150, Evalue=8e-38,
Organism=Escherichia coli, GI1787550, Length=273, Percent_Identity=26.3736263736264, Blast_Score=125, Evalue=3e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 33987; Mature: 33987

Theoretical pI: Translated: 10.23; Mature: 10.23

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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EECCCCCC
>Mature Secondary Structure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EECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1901616; 1899858; 9384377 [H]