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The map label for this gene is tyrS [H]

Identifier: 187933845

GI number: 187933845

Start: 3283324

End: 3284544

Strand: Reverse

Name: tyrS [H]

Synonym: CLL_A3166

Alternate gene names: 187933845

Gene position: 3284544-3283324 (Counterclockwise)

Preceding gene: 187933120

Following gene: 187934630

Centisome position: 86.43

GC content: 31.29

Gene sequence:

>1221_bases
ATGGCAAATGTATTAGATGAGTTACTTGATCGTGGGTACATAAAACAATTTACTCATGAAGAGGAAACAAGAAAATTATT
AGAAAATGAGAAGGTAACTTTTTATATAGGATTTGATCCAACTGCAGACAGTTTACATGTAGGTCACTTTATAGCGATGA
TGTTTATGGCTCATATGCAAAGAGCTGGACATAGACCGATAGCTTTATTAGGCGGTGGAACAGCGATGGTTGGAGATCCA
AGTGGTAAGACTGATATGAGAAAAATGCTTACTAAGGAACAAATACAACATAATGTTGATTCTATAAAAAAACAAATGGA
AAGATTTATAGACTTTTCTGATGATAAAGCCTTAATAGTAAACAATGCAGATTGGTTATTAGATCTTAATTATGTTGATT
TTTTAAGAGAAGTAGGAGTTCATTTCTCAGTTAATAGAATGTTAAGTGCAGAATGTTTTAAACAAAGATTAGAAAAAGGA
TTATCATTTTTAGAATTTAACTATATGTTAATGCAAGGATATGATTTTTATGTTTTAAATCAAAAATATGGATGTAAGAT
GGAACTTGGTGGAGATGATCAATGGTCTAACATGATTGCTGGTGTTGAGCTTGTAAGAAGAAAAGCACAAGGCGATGCGA
TGGCTATGACTTGTACATTATTAACTAATAGCCAAGGACAAAAAATGGGTAAAACTGTTGGTGGAGCATTATGGCTTGAT
GCTGATAAAGTATCTCCATTTGACTTTTATCAATATTGGAGAAATGTTGATGATGCAGATGTAGAAAAATGTTTAGCATT
ATTAACTTTCTTACCAATGGATGAAGTAAGAAGGTTAGGTGCTTTAGAAGGTGCTGAAATTAATGGAGCTAAGAAAATTC
TTGCTTATGAAGTTACAAAACTTGTTCATGGAGATGAAGAAGCTAAGAAAGCAGAAGAAGCAGCTAATGCTTTATTCTCA
GGTGGTGCTGACATGTCAAATGTACCAACAGTAATTATATCTAAAGAAGATTTAGGTTCAACAGTTTTAGATGTAATAGC
TAAAGTGAAAATAGTTCCATCTAAAAAAGAAGGAAGAAGATTAATAGAACAAGGTGGTTTATCTATTAATGGAGAAAAGA
TCACTGAATTAACAAGAACATTAAATGATGATGATTTTAAAGATGGATCTGCTTTAATAAAAAGAGGTAAGAAAAATTAT
AATAAAATAGAAATACAATAG

Upstream 100 bases:

>100_bases
CAAAATATAAGGATAACGATATACTATAAATATTGTAATTTAAATGTATATATATAGTAGATAATTGAACTTAGTTCAAT
AATTTAGGAGGTTTATAGTA

Downstream 100 bases:

>100_bases
TAAAATTATTGTTTTAAAAATAACATATTGTAATTAAAAAATAGGATTTGAAAAATTATTTCAAATCCTATTTTTTTAGG
TAATTTAATGGTTATTAAGA

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 406; Mature: 405

Protein sequence:

>406_residues
MANVLDELLDRGYIKQFTHEEETRKLLENEKVTFYIGFDPTADSLHVGHFIAMMFMAHMQRAGHRPIALLGGGTAMVGDP
SGKTDMRKMLTKEQIQHNVDSIKKQMERFIDFSDDKALIVNNADWLLDLNYVDFLREVGVHFSVNRMLSAECFKQRLEKG
LSFLEFNYMLMQGYDFYVLNQKYGCKMELGGDDQWSNMIAGVELVRRKAQGDAMAMTCTLLTNSQGQKMGKTVGGALWLD
ADKVSPFDFYQYWRNVDDADVEKCLALLTFLPMDEVRRLGALEGAEINGAKKILAYEVTKLVHGDEEAKKAEEAANALFS
GGADMSNVPTVIISKEDLGSTVLDVIAKVKIVPSKKEGRRLIEQGGLSINGEKITELTRTLNDDDFKDGSALIKRGKKNY
NKIEIQ

Sequences:

>Translated_406_residues
MANVLDELLDRGYIKQFTHEEETRKLLENEKVTFYIGFDPTADSLHVGHFIAMMFMAHMQRAGHRPIALLGGGTAMVGDP
SGKTDMRKMLTKEQIQHNVDSIKKQMERFIDFSDDKALIVNNADWLLDLNYVDFLREVGVHFSVNRMLSAECFKQRLEKG
LSFLEFNYMLMQGYDFYVLNQKYGCKMELGGDDQWSNMIAGVELVRRKAQGDAMAMTCTLLTNSQGQKMGKTVGGALWLD
ADKVSPFDFYQYWRNVDDADVEKCLALLTFLPMDEVRRLGALEGAEINGAKKILAYEVTKLVHGDEEAKKAEEAANALFS
GGADMSNVPTVIISKEDLGSTVLDVIAKVKIVPSKKEGRRLIEQGGLSINGEKITELTRTLNDDDFKDGSALIKRGKKNY
NKIEIQ
>Mature_405_residues
ANVLDELLDRGYIKQFTHEEETRKLLENEKVTFYIGFDPTADSLHVGHFIAMMFMAHMQRAGHRPIALLGGGTAMVGDPS
GKTDMRKMLTKEQIQHNVDSIKKQMERFIDFSDDKALIVNNADWLLDLNYVDFLREVGVHFSVNRMLSAECFKQRLEKGL
SFLEFNYMLMQGYDFYVLNQKYGCKMELGGDDQWSNMIAGVELVRRKAQGDAMAMTCTLLTNSQGQKMGKTVGGALWLDA
DKVSPFDFYQYWRNVDDADVEKCLALLTFLPMDEVRRLGALEGAEINGAKKILAYEVTKLVHGDEEAKKAEEAANALFSG
GADMSNVPTVIISKEDLGSTVLDVIAKVKIVPSKKEGRRLIEQGGLSINGEKITELTRTLNDDDFKDGSALIKRGKKNYN
KIEIQ

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=429, Percent_Identity=37.995337995338, Blast_Score=268, Evalue=6e-72,
Organism=Escherichia coli, GI1787925, Length=421, Percent_Identity=40.855106888361, Blast_Score=336, Evalue=2e-93,
Organism=Caenorhabditis elegans, GI25145802, Length=428, Percent_Identity=34.5794392523364, Blast_Score=230, Evalue=1e-60,
Organism=Caenorhabditis elegans, GI25145805, Length=327, Percent_Identity=33.6391437308869, Blast_Score=167, Evalue=7e-42,
Organism=Saccharomyces cerevisiae, GI6325160, Length=422, Percent_Identity=32.9383886255924, Blast_Score=224, Evalue=3e-59,
Organism=Drosophila melanogaster, GI20130383, Length=443, Percent_Identity=35.2144469525959, Blast_Score=282, Evalue=3e-76,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 45655; Mature: 45524

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS50889 S4 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANVLDELLDRGYIKQFTHEEETRKLLENEKVTFYIGFDPTADSLHVGHFIAMMFMAHMQ
CCHHHHHHHCCHHHHHHCCHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHH
RAGHRPIALLGGGTAMVGDPSGKTDMRKMLTKEQIQHNVDSIKKQMERFIDFSDDKALIV
HCCCCCEEEEECCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
NNADWLLDLNYVDFLREVGVHFSVNRMLSAECFKQRLEKGLSFLEFNYMLMQGYDFYVLN
ECCCEEEECHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHCCHHHHHHHHEECCCCEEEEE
QKYGCKMELGGDDQWSNMIAGVELVRRKAQGDAMAMTCTLLTNSQGQKMGKTVGGALWLD
CCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCHHHHHHHCCEEEEE
ADKVSPFDFYQYWRNVDDADVEKCLALLTFLPMDEVRRLGALEGAEINGAKKILAYEVTK
CCCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCHHHHHHHHHHH
LVHGDEEAKKAEEAANALFSGGADMSNVPTVIISKEDLGSTVLDVIAKVKIVPSKKEGRR
HHCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEECHHHHHHHHHHHHHHEECCCHHHHHH
LIEQGGLSINGEKITELTRTLNDDDFKDGSALIKRGKKNYNKIEIQ
HHHHCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCEEEEC
>Mature Secondary Structure 
ANVLDELLDRGYIKQFTHEEETRKLLENEKVTFYIGFDPTADSLHVGHFIAMMFMAHMQ
CHHHHHHHCCHHHHHHCCHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHH
RAGHRPIALLGGGTAMVGDPSGKTDMRKMLTKEQIQHNVDSIKKQMERFIDFSDDKALIV
HCCCCCEEEEECCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
NNADWLLDLNYVDFLREVGVHFSVNRMLSAECFKQRLEKGLSFLEFNYMLMQGYDFYVLN
ECCCEEEECHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHCCHHHHHHHHEECCCCEEEEE
QKYGCKMELGGDDQWSNMIAGVELVRRKAQGDAMAMTCTLLTNSQGQKMGKTVGGALWLD
CCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCHHHHHHHCCEEEEE
ADKVSPFDFYQYWRNVDDADVEKCLALLTFLPMDEVRRLGALEGAEINGAKKILAYEVTK
CCCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCHHHHHHHHHHH
LVHGDEEAKKAEEAANALFSGGADMSNVPTVIISKEDLGSTVLDVIAKVKIVPSKKEGRR
HHCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEECHHHHHHHHHHHHHHEECCCHHHHHH
LIEQGGLSINGEKITELTRTLNDDDFKDGSALIKRGKKNYNKIEIQ
HHHHCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA