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The map label for this gene is ybjI [H]

Identifier: 187933705

GI number: 187933705

Start: 996456

End: 997244

Strand: Reverse

Name: ybjI [H]

Synonym: CLL_A0985

Alternate gene names: 187933705

Gene position: 997244-996456 (Counterclockwise)

Preceding gene: 187934320

Following gene: 187933526

Centisome position: 26.24

GC content: 27.25

Gene sequence:

>789_bases
ATGATAAAATTAATCGCAACAGATATGGATGGAACTTTATTAGATGAAAAAGGACATTTACCAGAAAGCTTTTTAGAAGT
TTTAGATTTAATAACTGAAAAAAATGTTAAGTTAGTTATAGCTAGTGGTAGACCATATGCTACATTACAAACAAACTTTG
GACCAGTAGCTAAAAAATTATCATATATTACTGATAACGGAGCATTAGTATATGATAATAATAAATTAATATTTAAGGAT
GTTATAGAAAAAAACTTAGTACAAGATATAATAAAAACAGCTAGAAATATAGAAAATACTACGATAGTATTATGTGGGGT
AGAATGTGCCTATTTAGAAAATTGTTCTGAAGAATACTTAAAAGAAATACAAAAATATTATGTAAAATATGAAGTAGTTG
ATGATATATCTAAAGTTGAAGATGATATAATCAAGGTCACTTTATGTGATTTAGATCATGCAATAAAAAATTCAAACCCA
GTTATAAGCCCATTATTCGGAAATGATTTAAATGTAGTTGTAGCTGGTGAAATCTGGCTTGATATAATGAATAAAACAGT
AAACAAAGGTACTGCTTTAAGAAAAATAATGGAAGCTGATAATATCTCAAAAGAGGAAACAATGGCATTTGGAGATTACT
ATAATGATATAGAAATGCTAAATGAAGCTGACTTTAGCTTTGTTATGAAAAATGCTCCAGAAGATATGAAACAACATGCA
AAATACATAGCAGAAAGTAATGAAGATTATGGTGTATTAGGAGCTATAATGGAACATGTAGTTCTATAA

Upstream 100 bases:

>100_bases
TATCCTAAATTATGCTATATGCTGAATTCTTTAAAGATATTAACATTTATGATATGATGTAAATAATTAAATTAAGTACA
AGCACTTTGAGGAGGACTTT

Downstream 100 bases:

>100_bases
TAGCCAAGTGTGTTAATATATGTAATAGGCAAAATGGCATTGTAATTGCACTTTGAGAATCCTTAATGAGTATCGTCCCA
TTTACTGCTTGTCTTAATTT

Product: HAD hydrolase, IIB family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MIKLIATDMDGTLLDEKGHLPESFLEVLDLITEKNVKLVIASGRPYATLQTNFGPVAKKLSYITDNGALVYDNNKLIFKD
VIEKNLVQDIIKTARNIENTTIVLCGVECAYLENCSEEYLKEIQKYYVKYEVVDDISKVEDDIIKVTLCDLDHAIKNSNP
VISPLFGNDLNVVVAGEIWLDIMNKTVNKGTALRKIMEADNISKEETMAFGDYYNDIEMLNEADFSFVMKNAPEDMKQHA
KYIAESNEDYGVLGAIMEHVVL

Sequences:

>Translated_262_residues
MIKLIATDMDGTLLDEKGHLPESFLEVLDLITEKNVKLVIASGRPYATLQTNFGPVAKKLSYITDNGALVYDNNKLIFKD
VIEKNLVQDIIKTARNIENTTIVLCGVECAYLENCSEEYLKEIQKYYVKYEVVDDISKVEDDIIKVTLCDLDHAIKNSNP
VISPLFGNDLNVVVAGEIWLDIMNKTVNKGTALRKIMEADNISKEETMAFGDYYNDIEMLNEADFSFVMKNAPEDMKQHA
KYIAESNEDYGVLGAIMEHVVL
>Mature_262_residues
MIKLIATDMDGTLLDEKGHLPESFLEVLDLITEKNVKLVIASGRPYATLQTNFGPVAKKLSYITDNGALVYDNNKLIFKD
VIEKNLVQDIIKTARNIENTTIVLCGVECAYLENCSEEYLKEIQKYYVKYEVVDDISKVEDDIIKVTLCDLDHAIKNSNP
VISPLFGNDLNVVVAGEIWLDIMNKTVNKGTALRKIMEADNISKEETMAFGDYYNDIEMLNEADFSFVMKNAPEDMKQHA
KYIAESNEDYGVLGAIMEHVVL

Specific function: Catalyzes the dephosphorylation of the artificial chromogenic substrate p-nitrophenyl phosphate (pNPP) and of the natural substrates FMN and beta-glucose 1-phosphate [H]

COG id: COG0561

COG function: function code R; Predicted hydrolases of the HAD superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. Cof family [H]

Homologues:

Organism=Escherichia coli, GI87081790, Length=257, Percent_Identity=31.9066147859922, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI1787043, Length=265, Percent_Identity=29.811320754717, Blast_Score=124, Evalue=5e-30,
Organism=Escherichia coli, GI2367265, Length=282, Percent_Identity=27.6595744680851, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI87081741, Length=244, Percent_Identity=25, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI48994981, Length=239, Percent_Identity=25.1046025104602, Blast_Score=78, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR000150 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: NA

Molecular weight: Translated: 29533; Mature: 29533

Theoretical pI: Translated: 4.31; Mature: 4.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKLIATDMDGTLLDEKGHLPESFLEVLDLITEKNVKLVIASGRPYATLQTNFGPVAKKL
CEEEEEECCCCCEECCCCCCCHHHHHHHHHHHCCCEEEEEECCCCEEEEECCCCHHHHHH
SYITDNGALVYDNNKLIFKDVIEKNLVQDIIKTARNIENTTIVLCGVECAYLENCSEEYL
HHEECCCEEEEECCEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHCCHHHHH
KEIQKYYVKYEVVDDISKVEDDIIKVTLCDLDHAIKNSNPVISPLFGNDLNVVVAGEIWL
HHHHHHHHHHHHHHHHHHHHHHHEEEEEECHHHHHCCCCCEEEEECCCCCCEEEEHHHHH
DIMNKTVNKGTALRKIMEADNISKEETMAFGDYYNDIEMLNEADFSFVMKNAPEDMKQHA
HHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHH
KYIAESNEDYGVLGAIMEHVVL
HHHHCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MIKLIATDMDGTLLDEKGHLPESFLEVLDLITEKNVKLVIASGRPYATLQTNFGPVAKKL
CEEEEEECCCCCEECCCCCCCHHHHHHHHHHHCCCEEEEEECCCCEEEEECCCCHHHHHH
SYITDNGALVYDNNKLIFKDVIEKNLVQDIIKTARNIENTTIVLCGVECAYLENCSEEYL
HHEECCCEEEEECCEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHCCHHHHH
KEIQKYYVKYEVVDDISKVEDDIIKVTLCDLDHAIKNSNPVISPLFGNDLNVVVAGEIWL
HHHHHHHHHHHHHHHHHHHHHHHEEEEEECHHHHHCCCCCEEEEECCCCCCEEEEHHHHH
DIMNKTVNKGTALRKIMEADNISKEETMAFGDYYNDIEMLNEADFSFVMKNAPEDMKQHA
HHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHH
KYIAESNEDYGVLGAIMEHVVL
HHHHCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10493123 [H]