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The map label for this gene is nplT [H]

Identifier: 187933368

GI number: 187933368

Start: 2114602

End: 2116449

Strand: Reverse

Name: nplT [H]

Synonym: CLL_A2022

Alternate gene names: 187933368

Gene position: 2116449-2114602 (Counterclockwise)

Preceding gene: 187932396

Following gene: 187933427

Centisome position: 55.69

GC content: 25.38

Gene sequence:

>1848_bases
ATGAACTTATCTTCAATAATACATGAGCCTAAAAGCTCATATTGTTATGCATATAATAATGATACTATTCATATACGTTT
AAAAACCGCAAAAAATGAAATAAAGAAGGTATTACTTTTAGCTGTAGATCCATTTAATTGGGTTCCCAGGAAAGATAATC
CTCAAATATATGAGTTTGAAGTAGATTCTATTTTAAAAAATCAAATGGAAAAAGAACAAGTTACAGAACTATATGATTGC
TGGTTTGCGGAAATAAAAGGAATTGAGTGGGGAAGGATTAAATATTGTTTTATTGTAGAAAATAATAATGAAAAATTTAT
TATTGGAAGCCATGATAGAATTAAATATTCAGGAAATACAAAAGATCTTTATGATTTAAGTAATTATTTTAATTATCCAT
ATATAAATGAAGAAGATTTATATACATGTCCAGAATGGGTTTCAGAAACAGTATGGTATCAAATTTTCCCAGAAAGATTT
GCTAAAGGAAAAACTGATAGCAAAAAGGATTTTATTCCATGGGGAAATGATGACATAGATGGTGCTCATAAGAAATTTGG
CGGAAATCTTAATGGAGTAATAGATAAATTAGATTATATTAAAGAGACTGGATTTAGTGGAATTTATTTTAATCCTATTT
TTGAATCACGAAGTTCTCATAAATATGATACAACTAATTATTATAAAATAGATCCTGAATTTGGAGATAATGAAACATTT
AAAATTTTAGTAGAAGAAGCTCATAAACGAGGAATAAAAGTAATGCTAGATGGTGTATTTAATCATTGTGGATTTCTTCA
TCCTTTTTGGCAAGATGTGTGTAAGAATGGAATAAAATCTAAATATTATGATTGCTTTTATATCTTAGATCCATCAAAGC
CAATAGTTCAAGGAGATTTAAAGAATGGATTACCAGAGGAGTGCTCTAGAGAAAAATTAAATTATCGTACATTTGCTTTT
ACCCAAAGTATGCCAAAATGGAACACTAAAAATAAGATAGTGCGAGATTATTTGATTGATGTAGGATCTTATTGGGTTAA
AAATTATAATATAGATGGATGGAGATTAGATGTATCAAATGAAGTTTCACATGATTTTTGGAGAGAATTTAGAAAAAAAA
TAAAAAGTATAAATAAAGATGTATATATATTAGGGGAAAACTGGGATAATTCTTATCCTTGGCTTCAAGGTGATCAATTT
GATTCAGTTATGAATTATGAATTTACAAAGCCAGTTTGGAATTTTTTTAAAGAAAATAGTGAAGAAACTAAATATGATTC
AGAAAAATTTAAATTTTCTATTAGTAAGCTTTTAACTGATTATCCAAAGAATATAACAAAAAATATGTTTAATCTATTAG
ATAGTCATGACACAACAAGAATTTTCAATATTGTTGGAAAAAATACTGAAATTGCAAAATTAGCTTATGTTTTTATGATG
ACATTTCCAGGATGTCCTTGTATTTATTATGGAAGTGAAATTGGAATGGATGGTGATGAGCATTCTAATCGTAAATGTAT
GATTTGGGATGAAAGTAAACAAAATAGAGAATTGTTTTCTACTATAAAGAAATTAATTAATCTTAGAAAAAAATACAAGT
CTTTTAATACAGAAAATATTAAATGGATTAATAATGAGGAAAATTTGAATATTGTTTTGTATAAAAAATCAACAGATAAA
GAAACTTTATATGTGTTACTTAATAATTCTCATAATGAAGTAAATTTATTATTACCTGATGAAATGAAACAAAGTGAATA
TATAAATTGTTATTCAGATAAAATAATAGAATTACAAGAAAAATTATTAATAAAGCCATATGAATTTAAATTACTATTAA
AAAGTTAA

Upstream 100 bases:

>100_bases
CAAAGTGAATAATAATTTTAATTTTTTATAGCAACTTAATTTAGGTTTAAAACTTTTTAATAAGAAATAATATAATAAAA
GTAGTTTTAGGAGGAATAAC

Downstream 100 bases:

>100_bases
TAACTTAAGTTAGATGAGAATTAGAATATCAAGAGTAATAGAAGTTTTATCTTTAAACTTTTTAAATTTATATAACATCT
TCGTCATCTGTAATTTTATG

Product: neopullulanase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 615; Mature: 615

Protein sequence:

>615_residues
MNLSSIIHEPKSSYCYAYNNDTIHIRLKTAKNEIKKVLLLAVDPFNWVPRKDNPQIYEFEVDSILKNQMEKEQVTELYDC
WFAEIKGIEWGRIKYCFIVENNNEKFIIGSHDRIKYSGNTKDLYDLSNYFNYPYINEEDLYTCPEWVSETVWYQIFPERF
AKGKTDSKKDFIPWGNDDIDGAHKKFGGNLNGVIDKLDYIKETGFSGIYFNPIFESRSSHKYDTTNYYKIDPEFGDNETF
KILVEEAHKRGIKVMLDGVFNHCGFLHPFWQDVCKNGIKSKYYDCFYILDPSKPIVQGDLKNGLPEECSREKLNYRTFAF
TQSMPKWNTKNKIVRDYLIDVGSYWVKNYNIDGWRLDVSNEVSHDFWREFRKKIKSINKDVYILGENWDNSYPWLQGDQF
DSVMNYEFTKPVWNFFKENSEETKYDSEKFKFSISKLLTDYPKNITKNMFNLLDSHDTTRIFNIVGKNTEIAKLAYVFMM
TFPGCPCIYYGSEIGMDGDEHSNRKCMIWDESKQNRELFSTIKKLINLRKKYKSFNTENIKWINNEENLNIVLYKKSTDK
ETLYVLLNNSHNEVNLLLPDEMKQSEYINCYSDKIIELQEKLLIKPYEFKLLLKS

Sequences:

>Translated_615_residues
MNLSSIIHEPKSSYCYAYNNDTIHIRLKTAKNEIKKVLLLAVDPFNWVPRKDNPQIYEFEVDSILKNQMEKEQVTELYDC
WFAEIKGIEWGRIKYCFIVENNNEKFIIGSHDRIKYSGNTKDLYDLSNYFNYPYINEEDLYTCPEWVSETVWYQIFPERF
AKGKTDSKKDFIPWGNDDIDGAHKKFGGNLNGVIDKLDYIKETGFSGIYFNPIFESRSSHKYDTTNYYKIDPEFGDNETF
KILVEEAHKRGIKVMLDGVFNHCGFLHPFWQDVCKNGIKSKYYDCFYILDPSKPIVQGDLKNGLPEECSREKLNYRTFAF
TQSMPKWNTKNKIVRDYLIDVGSYWVKNYNIDGWRLDVSNEVSHDFWREFRKKIKSINKDVYILGENWDNSYPWLQGDQF
DSVMNYEFTKPVWNFFKENSEETKYDSEKFKFSISKLLTDYPKNITKNMFNLLDSHDTTRIFNIVGKNTEIAKLAYVFMM
TFPGCPCIYYGSEIGMDGDEHSNRKCMIWDESKQNRELFSTIKKLINLRKKYKSFNTENIKWINNEENLNIVLYKKSTDK
ETLYVLLNNSHNEVNLLLPDEMKQSEYINCYSDKIIELQEKLLIKPYEFKLLLKS
>Mature_615_residues
MNLSSIIHEPKSSYCYAYNNDTIHIRLKTAKNEIKKVLLLAVDPFNWVPRKDNPQIYEFEVDSILKNQMEKEQVTELYDC
WFAEIKGIEWGRIKYCFIVENNNEKFIIGSHDRIKYSGNTKDLYDLSNYFNYPYINEEDLYTCPEWVSETVWYQIFPERF
AKGKTDSKKDFIPWGNDDIDGAHKKFGGNLNGVIDKLDYIKETGFSGIYFNPIFESRSSHKYDTTNYYKIDPEFGDNETF
KILVEEAHKRGIKVMLDGVFNHCGFLHPFWQDVCKNGIKSKYYDCFYILDPSKPIVQGDLKNGLPEECSREKLNYRTFAF
TQSMPKWNTKNKIVRDYLIDVGSYWVKNYNIDGWRLDVSNEVSHDFWREFRKKIKSINKDVYILGENWDNSYPWLQGDQF
DSVMNYEFTKPVWNFFKENSEETKYDSEKFKFSISKLLTDYPKNITKNMFNLLDSHDTTRIFNIVGKNTEIAKLAYVFMM
TFPGCPCIYYGSEIGMDGDEHSNRKCMIWDESKQNRELFSTIKKLINLRKKYKSFNTENIKWINNEENLNIVLYKKSTDK
ETLYVLLNNSHNEVNLLLPDEMKQSEYINCYSDKIIELQEKLLIKPYEFKLLLKS

Specific function: Hydrolyzes pullulan efficiently but only a small amount of starch. Endohydrolysis of 1,4-alpha-glucosidic linkages in pullulan to form panose. Cleaves also (1-6)-alpha-glucosidic linkages to form maltotriose [H]

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI187423904, Length=408, Percent_Identity=22.7941176470588, Blast_Score=71, Evalue=3e-12,
Organism=Escherichia coli, GI1786604, Length=477, Percent_Identity=29.979035639413, Blast_Score=216, Evalue=3e-57,
Organism=Escherichia coli, GI1790687, Length=393, Percent_Identity=27.2264631043257, Blast_Score=103, Evalue=4e-23,
Organism=Escherichia coli, GI1789995, Length=152, Percent_Identity=26.9736842105263, Blast_Score=63, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6322245, Length=231, Percent_Identity=31.1688311688312, Blast_Score=95, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6321726, Length=230, Percent_Identity=28.2608695652174, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6324416, Length=230, Percent_Identity=27.8260869565217, Blast_Score=84, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6322241, Length=230, Percent_Identity=27.8260869565217, Blast_Score=84, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6322021, Length=230, Percent_Identity=27.8260869565217, Blast_Score=84, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6319776, Length=231, Percent_Identity=27.2727272727273, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6321731, Length=231, Percent_Identity=26.8398268398268, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24583745, Length=410, Percent_Identity=26.3414634146341, Blast_Score=98, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24586593, Length=412, Percent_Identity=24.0291262135922, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24586587, Length=225, Percent_Identity=29.7777777777778, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24583747, Length=233, Percent_Identity=29.6137339055794, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24583749, Length=233, Percent_Identity=29.6137339055794, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI221330053, Length=419, Percent_Identity=23.1503579952267, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24586591, Length=409, Percent_Identity=22.9828850855746, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24586599, Length=416, Percent_Identity=25, Blast_Score=83, Evalue=7e-16,
Organism=Drosophila melanogaster, GI45549022, Length=411, Percent_Identity=24.0875912408759, Blast_Score=80, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24586597, Length=409, Percent_Identity=22.4938875305623, Blast_Score=76, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24586589, Length=414, Percent_Identity=23.4299516908213, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004185
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02903 Alpha-amylase_N [H]

EC number: =3.2.1.135 [H]

Molecular weight: Translated: 73084; Mature: 73084

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLSSIIHEPKSSYCYAYNNDTIHIRLKTAKNEIKKVLLLAVDPFNWVPRKDNPQIYEFE
CCHHHHHCCCCCCEEEEECCCEEEEEEECCHHHHHEEEEEEECCCCCCCCCCCCCEEEEE
VDSILKNQMEKEQVTELYDCWFAEIKGIEWGRIKYCFIVENNNEKFIIGSHDRIKYSGNT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCEEEEECCCEEEECCCC
KDLYDLSNYFNYPYINEEDLYTCPEWVSETVWYQIFPERFAKGKTDSKKDFIPWGNDDID
CHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCC
GAHKKFGGNLNGVIDKLDYIKETGFSGIYFNPIFESRSSHKYDTTNYYKIDPEFGDNETF
CHHHHHCCCHHHHHHHHHHHHHCCCCCEEECHHHCCCCCCCCCCCCEEEECCCCCCCCEE
KILVEEAHKRGIKVMLDGVFNHCGFLHPFWQDVCKNGIKSKYYDCFYILDPSKPIVQGDL
EHEEHHHHHCCCEEEHHHHHHHCCCCCHHHHHHHHCCCCCCCEEEEEEECCCCCCEECHH
KNGLPEECSREKLNYRTFAFTQSMPKWNTKNKIVRDYLIDVGSYWVKNYNIDGWRLDVSN
HCCCCHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCC
EVSHDFWREFRKKIKSINKDVYILGENWDNSYPWLQGDQFDSVMNYEFTKPVWNFFKENS
CCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCHHHCCCCCCHHHHHHHHCCC
EETKYDSEKFKFSISKLLTDYPKNITKNMFNLLDSHDTTRIFNIVGKNTEIAKLAYVFMM
CCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHH
TFPGCPCIYYGSEIGMDGDEHSNRKCMIWDESKQNRELFSTIKKLINLRKKYKSFNTENI
HCCCCCEEEECCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCE
KWINNEENLNIVLYKKSTDKETLYVLLNNSHNEVNLLLPDEMKQSEYINCYSDKIIELQE
EEECCCCCEEEEEEECCCCCCEEEEEEECCCCEEEEEECCCCCCCCCEEHHHHHHHHHHH
KLLIKPYEFKLLLKS
HHCCCCEEEEEEECC
>Mature Secondary Structure
MNLSSIIHEPKSSYCYAYNNDTIHIRLKTAKNEIKKVLLLAVDPFNWVPRKDNPQIYEFE
CCHHHHHCCCCCCEEEEECCCEEEEEEECCHHHHHEEEEEEECCCCCCCCCCCCCEEEEE
VDSILKNQMEKEQVTELYDCWFAEIKGIEWGRIKYCFIVENNNEKFIIGSHDRIKYSGNT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCEEEEECCCEEEECCCC
KDLYDLSNYFNYPYINEEDLYTCPEWVSETVWYQIFPERFAKGKTDSKKDFIPWGNDDID
CHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCC
GAHKKFGGNLNGVIDKLDYIKETGFSGIYFNPIFESRSSHKYDTTNYYKIDPEFGDNETF
CHHHHHCCCHHHHHHHHHHHHHCCCCCEEECHHHCCCCCCCCCCCCEEEECCCCCCCCEE
KILVEEAHKRGIKVMLDGVFNHCGFLHPFWQDVCKNGIKSKYYDCFYILDPSKPIVQGDL
EHEEHHHHHCCCEEEHHHHHHHCCCCCHHHHHHHHCCCCCCCEEEEEEECCCCCCEECHH
KNGLPEECSREKLNYRTFAFTQSMPKWNTKNKIVRDYLIDVGSYWVKNYNIDGWRLDVSN
HCCCCHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCC
EVSHDFWREFRKKIKSINKDVYILGENWDNSYPWLQGDQFDSVMNYEFTKPVWNFFKENS
CCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCHHHCCCCCCHHHHHHHHCCC
EETKYDSEKFKFSISKLLTDYPKNITKNMFNLLDSHDTTRIFNIVGKNTEIAKLAYVFMM
CCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHH
TFPGCPCIYYGSEIGMDGDEHSNRKCMIWDESKQNRELFSTIKKLINLRKKYKSFNTENI
HCCCCCEEEECCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCE
KWINNEENLNIVLYKKSTDKETLYVLLNNSHNEVNLLLPDEMKQSEYINCYSDKIIELQE
EEECCCCCEEEEEEECCCCCCEEEEEEECCCCEEEEEECCCCCCCCCEEHHHHHHHHHHH
KLLIKPYEFKLLLKS
HHCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2482332; 12547200 [H]