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The map label for this gene is cycB [H]

Identifier: 187932396

GI number: 187932396

Start: 2116538

End: 2117812

Strand: Reverse

Name: cycB [H]

Synonym: CLL_A2023

Alternate gene names: 187932396

Gene position: 2117812-2116538 (Counterclockwise)

Preceding gene: 187933028

Following gene: 187933368

Centisome position: 55.73

GC content: 29.65

Gene sequence:

>1275_bases
ATGATAAAACAAATTAAAATGATATTTTTGCCAGTTTTGATAGTTACATTAATGTTCTCTTTAATATCATGTAGAAATAG
AGAAATACCATTTGATGATGATTCAGAATTAGAAGAAACATCAGAATTAAAGCCAGAAAAAGAAGCAGAATTAGTATATT
GGACAGATGATTTAGATTTTGGACAAGCAATAGCTAAATCCTTTGAAGAAAAATATAAAGTAAAAGTTAATGTTCAAAAG
ATAGGACTAGATTCAATGGATAAGATGATGCTAGATGCACCAACTGGTAATGGGGCTGATGTATTTATGGCAGCTAATGA
TAATTTTTCAAAAGGAAAGGATTCAGGAATTTTTGCTAAAATTAGACCATCAATAAAAAAAGAAATAGAAGAAGTAACCA
ATGAAACAGCTTTAAAAACTGTTACTAGTGATGATGAATTATATGGAATACCAGTTTCAATTGAAAGTTATGCAATGTTA
TATAACAAAGATTTAGTGAAAGAAAAACCCTCTGAAACATTTGAACAGATTGAAGAAGAGGCTAATAAGTATAATGATAA
AAATATTAATAAGTTTTGGTTTTTAACTATTGCTACTGATGGTTATTCAGCGTATCCGTTTTTAAGTGTAGATGGATTTA
GATTGTTTGGGGAAAATGGTAACGATGGAGATAATCCAGGATTTAATACTAAAGAATTTGAAAACGGCTTAGAAGCTATA
TCGAAACTAAGAAAAATTATTCCAATTGAATCAGAGGATTTAAAATTACAAGCAAGTACTTTTTTAGAACAGAATTTTAG
GAATGGAAAGACAGCATATTATCCAATAGGACCAAATGCTATAAAGGAATTGAAAAAATCGGGTGTTAATTTTGGAATTA
CTAAGTTACCAACTTATGGTGGAAAACCAATGAGAGCCTTTAGTGTTGTACAGAACGCGCATGTTTCAGCATATACAAAA
TATCCAAATGCATCACAACTTTTTGCACAATATTTAGTCTCTAAAGATGCAGCCTCTCTTTTATATAGAAAAGCATATAA
AATAACTTCAAGAAAAGATACATCTAATATAGAAGGTCTTAAAGATGATGAATATATGAAAGTGTATGCAGAGCAATTTA
ACAATACAGATCCAATGCCATCAACAAAAAGAATATCATATTTTTGGACAATAATGGAGAGTACATTAGGCTCTGTATTC
GATGGTAACATAACTCCTAATGAGGCGGCAGAAAAAATTCAAAAAGATTTTGATGCACTTGTACAAAGTGAATAA

Upstream 100 bases:

>100_bases
CCTATTACTATTGTATTTATTATTTTTCAAAGATACTTGGTACAAGGTGTTACATCAGGAGCAGATAAAGGTTGATTTTA
ATAAGGTATGGAGGAAAATT

Downstream 100 bases:

>100_bases
TAATTTTAATTTTTTATAGCAACTTAATTTAGGTTTAAAACTTTTTAATAAGAAATAATATAATAAAAGTAGTTTTAGGA
GGAATAACATGAACTTATCT

Product: extracellular solute-binding protein

Products: ADP; phosphate; maltose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 424; Mature: 424

Protein sequence:

>424_residues
MIKQIKMIFLPVLIVTLMFSLISCRNREIPFDDDSELEETSELKPEKEAELVYWTDDLDFGQAIAKSFEEKYKVKVNVQK
IGLDSMDKMMLDAPTGNGADVFMAANDNFSKGKDSGIFAKIRPSIKKEIEEVTNETALKTVTSDDELYGIPVSIESYAML
YNKDLVKEKPSETFEQIEEEANKYNDKNINKFWFLTIATDGYSAYPFLSVDGFRLFGENGNDGDNPGFNTKEFENGLEAI
SKLRKIIPIESEDLKLQASTFLEQNFRNGKTAYYPIGPNAIKELKKSGVNFGITKLPTYGGKPMRAFSVVQNAHVSAYTK
YPNASQLFAQYLVSKDAASLLYRKAYKITSRKDTSNIEGLKDDEYMKVYAEQFNNTDPMPSTKRISYFWTIMESTLGSVF
DGNITPNEAAEKIQKDFDALVQSE

Sequences:

>Translated_424_residues
MIKQIKMIFLPVLIVTLMFSLISCRNREIPFDDDSELEETSELKPEKEAELVYWTDDLDFGQAIAKSFEEKYKVKVNVQK
IGLDSMDKMMLDAPTGNGADVFMAANDNFSKGKDSGIFAKIRPSIKKEIEEVTNETALKTVTSDDELYGIPVSIESYAML
YNKDLVKEKPSETFEQIEEEANKYNDKNINKFWFLTIATDGYSAYPFLSVDGFRLFGENGNDGDNPGFNTKEFENGLEAI
SKLRKIIPIESEDLKLQASTFLEQNFRNGKTAYYPIGPNAIKELKKSGVNFGITKLPTYGGKPMRAFSVVQNAHVSAYTK
YPNASQLFAQYLVSKDAASLLYRKAYKITSRKDTSNIEGLKDDEYMKVYAEQFNNTDPMPSTKRISYFWTIMESTLGSVF
DGNITPNEAAEKIQKDFDALVQSE
>Mature_424_residues
MIKQIKMIFLPVLIVTLMFSLISCRNREIPFDDDSELEETSELKPEKEAELVYWTDDLDFGQAIAKSFEEKYKVKVNVQK
IGLDSMDKMMLDAPTGNGADVFMAANDNFSKGKDSGIFAKIRPSIKKEIEEVTNETALKTVTSDDELYGIPVSIESYAML
YNKDLVKEKPSETFEQIEEEANKYNDKNINKFWFLTIATDGYSAYPFLSVDGFRLFGENGNDGDNPGFNTKEFENGLEAI
SKLRKIIPIESEDLKLQASTFLEQNFRNGKTAYYPIGPNAIKELKKSGVNFGITKLPTYGGKPMRAFSVVQNAHVSAYTK
YPNASQLFAQYLVSKDAASLLYRKAYKITSRKDTSNIEGLKDDEYMKVYAEQFNNTDPMPSTKRISYFWTIMESTLGSVF
DGNITPNEAAEKIQKDFDALVQSE

Specific function: Binding protein for cyclodextrin; involved in its cellular uptake. Interacts with all natural cyclodextrins:alpha, beta and gamma [H]

COG id: COG2182

COG function: function code G; Maltose-binding periplasmic proteins/domains

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790466, Length=388, Percent_Identity=26.8041237113402, Blast_Score=96, Evalue=4e-21,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 720 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006060
- InterPro:   IPR006059 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 47983; Mature: 47983

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01037 SBP_BACTERIAL_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKQIKMIFLPVLIVTLMFSLISCRNREIPFDDDSELEETSELKPEKEAELVYWTDDLDF
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCCCEEEEECCCCH
GQAIAKSFEEKYKVKVNVQKIGLDSMDKMMLDAPTGNGADVFMAANDNFSKGKDSGIFAK
HHHHHHHHHHHEEEEEEEEECCCCHHHHHEEECCCCCCCEEEEEECCCCCCCCCCCEEEE
IRPSIKKEIEEVTNETALKTVTSDDELYGIPVSIESYAMLYNKDLVKEKPSETFEQIEEE
ECHHHHHHHHHHHHHHHHEEECCCCCEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHH
ANKYNDKNINKFWFLTIATDGYSAYPFLSVDGFRLFGENGNDGDNPGFNTKEFENGLEAI
HHHCCCCCCCEEEEEEEEECCCCCCCEEEECCEEEECCCCCCCCCCCCCHHHHHHHHHHH
SKLRKIIPIESEDLKLQASTFLEQNFRNGKTAYYPIGPNAIKELKKSGVNFGITKLPTYG
HHHHHHCCCCCCCCEEHHHHHHHHHCCCCCEEEEECCHHHHHHHHHCCCCCCEEECCCCC
GKPMRAFSVVQNAHVSAYTKYPNASQLFAQYLVSKDAASLLYRKAYKITSRKDTSNIEGL
CCCHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
KDDEYMKVYAEQFNNTDPMPSTKRISYFWTIMESTLGSVFDGNITPNEAAEKIQKDFDAL
CCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
VQSE
HCCC
>Mature Secondary Structure
MIKQIKMIFLPVLIVTLMFSLISCRNREIPFDDDSELEETSELKPEKEAELVYWTDDLDF
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCCCEEEEECCCCH
GQAIAKSFEEKYKVKVNVQKIGLDSMDKMMLDAPTGNGADVFMAANDNFSKGKDSGIFAK
HHHHHHHHHHHEEEEEEEEECCCCHHHHHEEECCCCCCCEEEEEECCCCCCCCCCCEEEE
IRPSIKKEIEEVTNETALKTVTSDDELYGIPVSIESYAMLYNKDLVKEKPSETFEQIEEE
ECHHHHHHHHHHHHHHHHEEECCCCCEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHH
ANKYNDKNINKFWFLTIATDGYSAYPFLSVDGFRLFGENGNDGDNPGFNTKEFENGLEAI
HHHCCCCCCCEEEEEEEEECCCCCCCEEEECCEEEECCCCCCCCCCCCCHHHHHHHHHHH
SKLRKIIPIESEDLKLQASTFLEQNFRNGKTAYYPIGPNAIKELKKSGVNFGITKLPTYG
HHHHHHCCCCCCCCEEHHHHHHHHHCCCCCEEEEECCHHHHHHHHHCCCCCCEEECCCCC
GKPMRAFSVVQNAHVSAYTKYPNASQLFAQYLVSKDAASLLYRKAYKITSRKDTSNIEGL
CCCHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
KDDEYMKVYAEQFNNTDPMPSTKRISYFWTIMESTLGSVFDGNITPNEAAEKIQKDFDAL
CCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
VQSE
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; maltose [Periplasm]; H2O [C]

Specific reaction: ATP + maltose [Periplasm] + H2O = ADP + phosphate + maltose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]