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The map label for this gene is thiE

Identifier: 187933174

GI number: 187933174

Start: 3212001

End: 3212642

Strand: Reverse

Name: thiE

Synonym: CLL_A3104

Alternate gene names: 187933174

Gene position: 3212642-3212001 (Counterclockwise)

Preceding gene: 187935001

Following gene: 187933156

Centisome position: 84.54

GC content: 28.66

Gene sequence:

>642_bases
ATGAAAAATAAAATAGATTATAGTATTTATCTTGTTACAGATAGAGATTTAATGAGTACTAATACTTTAGAAGAAGCTGT
AGAAAAATCAATACTTGGAGGAACTACATTAGTTCAATTAAGAGAAAAAGAATGTTCATCACATGATTTTTATGAAACAG
CATTAAATGTAAAAAAAATAACACAAAAATACAATATACCATTAATAATAAACGATAGAGTTGATATAGCTTTAGCTGTT
GATGCAGATGGAATTCATATAGGTCAAAGTGATTTGCCAGCAACTGTTGTGAGAAATATAATTGGGGAAGATAAAATTCT
TGGAGTTTCAGCTGGTAATTTAGATGAAGCCTTAAAGGCACAAAAGGATGGAGCAGATTATATAGGGGTTGGAGCAATGT
ATTCCACTGGAACGAAGAAAGATGCAACATCAACAACTATGAATGAATTAAGGGAAATAATGAAAAAAGTATCAATACCT
GTAGTTGTGATAGGGGGAATAAATAAGGAACGAATTAAAGATTTTAATGGAATAAATATAGATGGATTAGCTATAGTATC
AGCTATTATAGCACAAGAAAACATTGAAAAATCTACAAGAGAATTAAAAGAAGAATTTGATAAATTAAATACTCTAATAT
AG

Upstream 100 bases:

>100_bases
TTATATTAAAAATTTAATTAAGAAATATAAAATAATTATTGTTAATTATAATGGATATTAAATCTTACAAAGTATATTAT
TATTGAAATGAGGCAGATTT

Downstream 100 bases:

>100_bases
TTAAATTAAGAATTATATAAGACAAACTATTGCCGTATAGAATAAAGTTCTATGCGGTTTTTTCTATTAAATAAGGTCAT
TTATAGTTGTGTGATTGGAA

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MKNKIDYSIYLVTDRDLMSTNTLEEAVEKSILGGTTLVQLREKECSSHDFYETALNVKKITQKYNIPLIINDRVDIALAV
DADGIHIGQSDLPATVVRNIIGEDKILGVSAGNLDEALKAQKDGADYIGVGAMYSTGTKKDATSTTMNELREIMKKVSIP
VVVIGGINKERIKDFNGINIDGLAIVSAIIAQENIEKSTRELKEEFDKLNTLI

Sequences:

>Translated_213_residues
MKNKIDYSIYLVTDRDLMSTNTLEEAVEKSILGGTTLVQLREKECSSHDFYETALNVKKITQKYNIPLIINDRVDIALAV
DADGIHIGQSDLPATVVRNIIGEDKILGVSAGNLDEALKAQKDGADYIGVGAMYSTGTKKDATSTTMNELREIMKKVSIP
VVVIGGINKERIKDFNGINIDGLAIVSAIIAQENIEKSTRELKEEFDKLNTLI
>Mature_213_residues
MKNKIDYSIYLVTDRDLMSTNTLEEAVEKSILGGTTLVQLREKECSSHDFYETALNVKKITQKYNIPLIINDRVDIALAV
DADGIHIGQSDLPATVVRNIIGEDKILGVSAGNLDEALKAQKDGADYIGVGAMYSTGTKKDATSTTMNELREIMKKVSIP
VVVIGGINKERIKDFNGINIDGLAIVSAIIAQENIEKSTRELKEEFDKLNTLI

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP)

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family

Homologues:

Organism=Escherichia coli, GI1790426, Length=185, Percent_Identity=29.1891891891892, Blast_Score=70, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6325042, Length=226, Percent_Identity=39.8230088495575, Blast_Score=158, Evalue=6e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIE_CLOBB (B2TQ13)

Other databases:

- EMBL:   CP001056
- RefSeq:   YP_001887290.1
- ProteinModelPortal:   B2TQ13
- GeneID:   6293448
- GenomeReviews:   CP001056_GR
- KEGG:   cbk:CLL_A3104
- HOGENOM:   HBG754477
- OMA:   SAICHAE
- ProtClustDB:   CLSK912214
- HAMAP:   MF_00097
- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00693

Pfam domain/function: PF02581 TMP-TENI; SSF51391 TMP_synthase

EC number: =2.5.1.3

Molecular weight: Translated: 23388; Mature: 23388

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNKIDYSIYLVTDRDLMSTNTLEEAVEKSILGGTTLVQLREKECSSHDFYETALNVKKI
CCCCEEEEEEEEECCCHHHHHHHHHHHHHHHCCCHHHEEEHHHCCCCCHHHHHHHHHHHH
TQKYNIPLIINDRVDIALAVDADGIHIGQSDLPATVVRNIIGEDKILGVSAGNLDEALKA
HHHCCCCEEECCCCEEEEEECCCCEEECCCCCHHHHHHHHHCCCCEEEECCCCHHHHHHH
QKDGADYIGVGAMYSTGTKKDATSTTMNELREIMKKVSIPVVVIGGINKERIKDFNGINI
HCCCCCEEEECCEECCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHCCCCCC
DGLAIVSAIIAQENIEKSTRELKEEFDKLNTLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKNKIDYSIYLVTDRDLMSTNTLEEAVEKSILGGTTLVQLREKECSSHDFYETALNVKKI
CCCCEEEEEEEEECCCHHHHHHHHHHHHHHHCCCHHHEEEHHHCCCCCHHHHHHHHHHHH
TQKYNIPLIINDRVDIALAVDADGIHIGQSDLPATVVRNIIGEDKILGVSAGNLDEALKA
HHHCCCCEEECCCCEEEEEECCCCEEECCCCCHHHHHHHHHCCCCEEEECCCCHHHHHHH
QKDGADYIGVGAMYSTGTKKDATSTTMNELREIMKKVSIPVVVIGGINKERIKDFNGINI
HCCCCCEEEECCEECCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHCCCCCC
DGLAIVSAIIAQENIEKSTRELKEEFDKLNTLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA