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The map label for this gene is dapF [H]

Identifier: 187932919

GI number: 187932919

Start: 2512190

End: 2513011

Strand: Reverse

Name: dapF [H]

Synonym: CLL_A2428

Alternate gene names: 187932919

Gene position: 2513011-2512190 (Counterclockwise)

Preceding gene: 187934404

Following gene: 187935164

Centisome position: 66.13

GC content: 29.2

Gene sequence:

>822_bases
ATGGAATTTTATAAGATGCATGGAGCAGGAAATGATTTTATTTTTATTGAAGACTTTAATTGTGAAATTAAAGATGAATG
TAATTTAGCAAAAAAGGTTTGTGATAGACATTTTGGAATTGGAGCTGATGGCTTAGTTATTGTTAGAAAATCAGAAAATT
CATCAGCAAAAATGGTTATTATAAATTCTGATGGCTCTAGAGCAAATATGTGTGGAAATGCAATAAGGTGCTTTGGAAAA
TATGTTTATGAAAATAAGATCATAAATAAACTTAAGTTTCCAGTTGAAACAGGAGATGGTATTAAGGAAATAGAATTAAT
ACTAAGAGACGATAAAGTAGAATATATAAAAGTGTATATGGGAAAACCATCTTTTAATGGAAGAGATATATCATTAAATA
ATTTAGAAGAATTGATTGATAAAGAAGTTGTTATAGATAAAGAAAAATATAATATGACAAGTTTACTAATGGGAGTACCA
CATACTATTATTTTTGAAAGAGATAAGGAATATGAAGTTCAAGAAGGCGAGAAGATAGAAAAATTTTCTTTATTTAAAGA
AGGAACAAATGTTGATTTTGTAAAGGTTAATGATGAAAATCATATACGTGTAAATACATGGGAAAGAGGAGCAGGAGCAA
CACTTGCTTGCGGGACTGGATGCTGTGCTTCAGTTGTAGTTGCAAAGAAACTAGGATATGTAAATGGAGAAAATATATTT
GTAAAGGCTCCAGGTGGAAATCTTATAATAGAAGTTCTAGAAGATGGGGTATACATGAGTGGACCAGCAGAATTTATTTG
TAAAGGAAATATTATTTTATAG

Upstream 100 bases:

>100_bases
ATAATTTAATATAAGATAAGTTCAAATGTCATTGGGGCATTAAATTAAATTTATAATAATTATTTAAGTAAAAATAAGAT
AAATTGGGGGAGAGACTAGA

Downstream 100 bases:

>100_bases
CTATAAATTAATTGAGGGGATAAAAAATGAGCGTGTTAAAAAAGAAAAAAATGAGTATTCTAGTATTTATTGTTCTACCT
CTGCTGATGCTTGGATGTTC

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase [H]

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MEFYKMHGAGNDFIFIEDFNCEIKDECNLAKKVCDRHFGIGADGLVIVRKSENSSAKMVIINSDGSRANMCGNAIRCFGK
YVYENKIINKLKFPVETGDGIKEIELILRDDKVEYIKVYMGKPSFNGRDISLNNLEELIDKEVVIDKEKYNMTSLLMGVP
HTIIFERDKEYEVQEGEKIEKFSLFKEGTNVDFVKVNDENHIRVNTWERGAGATLACGTGCCASVVVAKKLGYVNGENIF
VKAPGGNLIIEVLEDGVYMSGPAEFICKGNIIL

Sequences:

>Translated_273_residues
MEFYKMHGAGNDFIFIEDFNCEIKDECNLAKKVCDRHFGIGADGLVIVRKSENSSAKMVIINSDGSRANMCGNAIRCFGK
YVYENKIINKLKFPVETGDGIKEIELILRDDKVEYIKVYMGKPSFNGRDISLNNLEELIDKEVVIDKEKYNMTSLLMGVP
HTIIFERDKEYEVQEGEKIEKFSLFKEGTNVDFVKVNDENHIRVNTWERGAGATLACGTGCCASVVVAKKLGYVNGENIF
VKAPGGNLIIEVLEDGVYMSGPAEFICKGNIIL
>Mature_273_residues
MEFYKMHGAGNDFIFIEDFNCEIKDECNLAKKVCDRHFGIGADGLVIVRKSENSSAKMVIINSDGSRANMCGNAIRCFGK
YVYENKIINKLKFPVETGDGIKEIELILRDDKVEYIKVYMGKPSFNGRDISLNNLEELIDKEVVIDKEKYNMTSLLMGVP
HTIIFERDKEYEVQEGEKIEKFSLFKEGTNVDFVKVNDENHIRVNTWERGAGATLACGTGCCASVVVAKKLGYVNGENIF
VKAPGGNLIIEVLEDGVYMSGPAEFICKGNIIL

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family [H]

Homologues:

Organism=Escherichia coli, GI87082334, Length=281, Percent_Identity=33.0960854092527, Blast_Score=144, Evalue=5e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001653
- InterPro:   IPR018510 [H]

Pfam domain/function: PF01678 DAP_epimerase [H]

EC number: =5.1.1.7 [H]

Molecular weight: Translated: 30555; Mature: 30555

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFYKMHGAGNDFIFIEDFNCEIKDECNLAKKVCDRHFGIGADGLVIVRKSENSSAKMVI
CCEEEECCCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEE
INSDGSRANMCGNAIRCFGKYVYENKIINKLKFPVETGDGIKEIELILRDDKVEYIKVYM
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCEEEEEEEECCCEEEEEEEE
GKPSFNGRDISLNNLEELIDKEVVIDKEKYNMTSLLMGVPHTIIFERDKEYEVQEGEKIE
CCCCCCCCEEEHHHHHHHHCHHEEEECCCCCHHHHHHCCCEEEEEECCCCCCCCCCCCCH
KFSLFKEGTNVDFVKVNDENHIRVNTWERGAGATLACGTGCCASVVVAKKLGYVNGENIF
HHHHEECCCCEEEEEECCCCEEEEEEECCCCCCEEEECCCHHHHHHHHHHCCCCCCCEEE
VKAPGGNLIIEVLEDGVYMSGPAEFICKGNIIL
EECCCCCEEEEEECCCEEECCCCCEEEECCEEC
>Mature Secondary Structure
MEFYKMHGAGNDFIFIEDFNCEIKDECNLAKKVCDRHFGIGADGLVIVRKSENSSAKMVI
CCEEEECCCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEE
INSDGSRANMCGNAIRCFGKYVYENKIINKLKFPVETGDGIKEIELILRDDKVEYIKVYM
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCEEEEEEEECCCEEEEEEEE
GKPSFNGRDISLNNLEELIDKEVVIDKEKYNMTSLLMGVPHTIIFERDKEYEVQEGEKIE
CCCCCCCCEEEHHHHHHHHCHHEEEECCCCCHHHHHHCCCEEEEEECCCCCCCCCCCCCH
KFSLFKEGTNVDFVKVNDENHIRVNTWERGAGATLACGTGCCASVVVAKKLGYVNGENIF
HHHHEECCCCEEEEEECCCCEEEEEEECCCCCCEEEECCCHHHHHHHHHHCCCCCCCEEE
VKAPGGNLIIEVLEDGVYMSGPAEFICKGNIIL
EECCCCCEEEEEECCCEEECCCCCEEEECCEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11792842; 7559358 [H]