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The map label for this gene is purA [H]

Identifier: 187932377

GI number: 187932377

Start: 3773452

End: 3774738

Strand: Reverse

Name: purA [H]

Synonym: CLL_A3576

Alternate gene names: 187932377

Gene position: 3774738-3773452 (Counterclockwise)

Preceding gene: 187934225

Following gene: 187934966

Centisome position: 99.33

GC content: 31.55

Gene sequence:

>1287_bases
ATGTCAGCATTTATTGTTTTAGGAGCCCAATGGGGAGATGAAGGAAAAGGAAAGATGACAGATTATTTAGCGGAAGAGGC
TGAAGTTGTTGTTAGATTTCAAGGAGGTAATAATGCTGGTCATACTGTAGAAGTTGGAGATAAGCAATATAAATTGCACT
TAATTCCATCAGGAATACTATATGATGATAAATTAAATGTTATTGGAAATGGAGTAGTAGTAGATCCTAAAGCTCTATTT
GAAGAAATAAATTATTTAGAAGGTGTTGGAGTTAATGTTACACCAGAAAAATTAATCATAAGTGATAGAGCACAACTTAT
AATGCCATATCATAAAACACTAGATGTTTTAAAAGAAAAAGCAAGAGGTAAAAATGATATAGGAACTACAGGAAAGGGCA
TAGGTCCATGTTACACAGACAAATTTGAAAGATGTGGAATTAGAGTTTGTGACCTTATGCATGAAGATGTTTTTAAAGAA
AAATTAGAAGAAAATATAAGAATGAAAAATGAGTATATTACTAAGGTATTAGGTGGAGAGCCTTTAAGTTTTAGTGAAAT
ATTAAATGAATATTTAGAATTTGCAAAGAAATTAAGACCTTTTGTTCAAGATACTTCAGTTAAGGTTTATAACAATATTA
AAGAAAATAAAACTGTACTATTTGAAGGTGCACAAGGTATGCTACTAGATATAGATTACGGAACATATCCTTATGTTACA
TCATCTAATACAACAGCTGGTGGTGTATGTAGTGGTATAGGTATTGGACCTAACATGGTAACTAATGCAGTAGGAATAAC
AAAAGCATATACTACAAGAGTTGGTAAAGGACCATTCCCAACAGAATTAGTTGATGAAACTGGAGATTGGATAAGAGAAA
AAGGTCATGAATATGGAGTAACAACAGGAAGATCAAGAAGATGTGGTTGGTTAGACTTAGTTATAGTTAAAACCGCTGCA
AGAGTAAGTGGATTGACTTCATTAGCTGTTACTAAGATAGATACATTAGCAGGATTAGAAAAATTAAAAGTATGTGTAGG
ATATAAATTTGATGGTAAAGTTATAGATTATTTCCCAGCAAGTTTAGAAGATCTAGCTAAATGCGAACCAGTATATGAAG
AATTTGACGGTTGGGATGATAGTGTAGCAGAAGCTAGAACATATGAAGAACTACCTGAAAATGCTAAGAAATACTTAAAT
AGAATAGCTGAATTTACAGATACTAAGATTTCTATAATAGGTGTTGGACCAAAGAGAGAACAAACTATAAGAATAGATAG
TATATAA

Upstream 100 bases:

>100_bases
CTTTGCTAATATTTAAAAGGAGAAGTTATTTAAATATTAAATAAACATCCAAATATTTTTATATAAAGCTATTAAAGCTT
GTATTTAAGGAGGCAAACAA

Downstream 100 bases:

>100_bases
TATTAAGTTTTCATGTTATTGACTTTACTTTTAGTATAAAATATAATTCATAGTAGATAATATTTGTATGAATTGATTTT
TGTAAATAAGAAGGTGAAAA

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MSAFIVLGAQWGDEGKGKMTDYLAEEAEVVVRFQGGNNAGHTVEVGDKQYKLHLIPSGILYDDKLNVIGNGVVVDPKALF
EEINYLEGVGVNVTPEKLIISDRAQLIMPYHKTLDVLKEKARGKNDIGTTGKGIGPCYTDKFERCGIRVCDLMHEDVFKE
KLEENIRMKNEYITKVLGGEPLSFSEILNEYLEFAKKLRPFVQDTSVKVYNNIKENKTVLFEGAQGMLLDIDYGTYPYVT
SSNTTAGGVCSGIGIGPNMVTNAVGITKAYTTRVGKGPFPTELVDETGDWIREKGHEYGVTTGRSRRCGWLDLVIVKTAA
RVSGLTSLAVTKIDTLAGLEKLKVCVGYKFDGKVIDYFPASLEDLAKCEPVYEEFDGWDDSVAEARTYEELPENAKKYLN
RIAEFTDTKISIIGVGPKREQTIRIDSI

Sequences:

>Translated_428_residues
MSAFIVLGAQWGDEGKGKMTDYLAEEAEVVVRFQGGNNAGHTVEVGDKQYKLHLIPSGILYDDKLNVIGNGVVVDPKALF
EEINYLEGVGVNVTPEKLIISDRAQLIMPYHKTLDVLKEKARGKNDIGTTGKGIGPCYTDKFERCGIRVCDLMHEDVFKE
KLEENIRMKNEYITKVLGGEPLSFSEILNEYLEFAKKLRPFVQDTSVKVYNNIKENKTVLFEGAQGMLLDIDYGTYPYVT
SSNTTAGGVCSGIGIGPNMVTNAVGITKAYTTRVGKGPFPTELVDETGDWIREKGHEYGVTTGRSRRCGWLDLVIVKTAA
RVSGLTSLAVTKIDTLAGLEKLKVCVGYKFDGKVIDYFPASLEDLAKCEPVYEEFDGWDDSVAEARTYEELPENAKKYLN
RIAEFTDTKISIIGVGPKREQTIRIDSI
>Mature_427_residues
SAFIVLGAQWGDEGKGKMTDYLAEEAEVVVRFQGGNNAGHTVEVGDKQYKLHLIPSGILYDDKLNVIGNGVVVDPKALFE
EINYLEGVGVNVTPEKLIISDRAQLIMPYHKTLDVLKEKARGKNDIGTTGKGIGPCYTDKFERCGIRVCDLMHEDVFKEK
LEENIRMKNEYITKVLGGEPLSFSEILNEYLEFAKKLRPFVQDTSVKVYNNIKENKTVLFEGAQGMLLDIDYGTYPYVTS
SNTTAGGVCSGIGIGPNMVTNAVGITKAYTTRVGKGPFPTELVDETGDWIREKGHEYGVTTGRSRRCGWLDLVIVKTAAR
VSGLTSLAVTKIDTLAGLEKLKVCVGYKFDGKVIDYFPASLEDLAKCEPVYEEFDGWDDSVAEARTYEELPENAKKYLNR
IAEFTDTKISIIGVGPKREQTIRIDSI

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI22748717, Length=427, Percent_Identity=46.8384074941452, Blast_Score=378, Evalue=1e-105,
Organism=Homo sapiens, GI34577063, Length=429, Percent_Identity=45.4545454545455, Blast_Score=364, Evalue=1e-101,
Organism=Homo sapiens, GI40316944, Length=398, Percent_Identity=45.4773869346734, Blast_Score=337, Evalue=1e-92,
Organism=Escherichia coli, GI1790620, Length=420, Percent_Identity=47.8571428571429, Blast_Score=397, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI25146553, Length=427, Percent_Identity=44.9648711943794, Blast_Score=352, Evalue=1e-97,
Organism=Caenorhabditis elegans, GI25146555, Length=427, Percent_Identity=44.9648711943794, Blast_Score=352, Evalue=2e-97,
Organism=Saccharomyces cerevisiae, GI6324109, Length=427, Percent_Identity=43.559718969555, Blast_Score=368, Evalue=1e-102,
Organism=Drosophila melanogaster, GI21356033, Length=423, Percent_Identity=42.789598108747, Blast_Score=364, Evalue=1e-101,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 47393; Mature: 47261

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1 ; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAFIVLGAQWGDEGKGKMTDYLAEEAEVVVRFQGGNNAGHTVEVGDKQYKLHLIPSGIL
CCEEEEEEECCCCCCCCHHHHHHHCCEEEEEEEECCCCCCCEEEECCCEEEEEEECCCEE
YDDKLNVIGNGVVVDPKALFEEINYLEGVGVNVTPEKLIISDRAQLIMPYHKTLDVLKEK
ECCCCCEEECCEEECHHHHHHHHHHHCCCCCCCCHHHHEECCCHHEEECHHHHHHHHHHH
ARGKNDIGTTGKGIGPCYTDKFERCGIRVCDLMHEDVFKEKLEENIRMKNEYITKVLGGE
HCCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PLSFSEILNEYLEFAKKLRPFVQDTSVKVYNNIKENKTVLFEGAQGMLLDIDYGTYPYVT
CCCHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCEEEEECCCCEEEEECCCCCCEEE
SSNTTAGGVCSGIGIGPNMVTNAVGITKAYTTRVGKGPFPTELVDETGDWIREKGHEYGV
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCC
TTGRSRRCGWLDLVIVKTAARVSGLTSLAVTKIDTLAGLEKLKVCVGYKFDGKVIDYFPA
CCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEECCCC
SLEDLAKCEPVYEEFDGWDDSVAEARTYEELPENAKKYLNRIAEFTDTKISIIGVGPKRE
CHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCC
QTIRIDSI
CEEEECCC
>Mature Secondary Structure 
SAFIVLGAQWGDEGKGKMTDYLAEEAEVVVRFQGGNNAGHTVEVGDKQYKLHLIPSGIL
CEEEEEEECCCCCCCCHHHHHHHCCEEEEEEEECCCCCCCEEEECCCEEEEEEECCCEE
YDDKLNVIGNGVVVDPKALFEEINYLEGVGVNVTPEKLIISDRAQLIMPYHKTLDVLKEK
ECCCCCEEECCEEECHHHHHHHHHHHCCCCCCCCHHHHEECCCHHEEECHHHHHHHHHHH
ARGKNDIGTTGKGIGPCYTDKFERCGIRVCDLMHEDVFKEKLEENIRMKNEYITKVLGGE
HCCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PLSFSEILNEYLEFAKKLRPFVQDTSVKVYNNIKENKTVLFEGAQGMLLDIDYGTYPYVT
CCCHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCEEEEECCCCEEEEECCCCCCEEE
SSNTTAGGVCSGIGIGPNMVTNAVGITKAYTTRVGKGPFPTELVDETGDWIREKGHEYGV
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCC
TTGRSRRCGWLDLVIVKTAARVSGLTSLAVTKIDTLAGLEKLKVCVGYKFDGKVIDYFPA
CCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEECCCC
SLEDLAKCEPVYEEFDGWDDSVAEARTYEELPENAKKYLNRIAEFTDTKISIIGVGPKRE
CHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCC
QTIRIDSI
CEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA