| Definition | Clostridium botulinum B str. Eklund 17B, complete genome. |
|---|---|
| Accession | NC_010674 |
| Length | 3,800,327 |
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The map label for this gene is hypF [H]
Identifier: 187932358
GI number: 187932358
Start: 2083894
End: 2086245
Strand: Reverse
Name: hypF [H]
Synonym: CLL_A2004
Alternate gene names: 187932358
Gene position: 2086245-2083894 (Counterclockwise)
Preceding gene: 187932743
Following gene: 187935094
Centisome position: 54.9
GC content: 25.51
Gene sequence:
>2352_bases ATGATTAAGAGAAAATATATTTTGGTAAATGGAAGAGTTCAAGGCGTTGGATTTAGGCCTTTTGTATATAGAATTGCATG TAAAAATAATATAAAAGGATATGTAAAAAATACTTGTAATGGTGTGCAAATTGATGTAGAGGGATTAGAAGAAAATATAA AAAGTTTTATCATGGATTTAGATAGTAAATGTCCAAAAGTTTGTAAGATTAATGAAATAGATATAAATGATATTAAGTAT ATGAATTATTATAATGATTTTCAAATACTAGAAAGTGATAATAATTATAAAGGTGAAACTTTAATTTCCCCAGATATAGC TATTTGTGATGAATGTTATAAAGAACTCTTTAATATTAGTGAAGAGAGGAGATATCTATATTCATTTATCAATTGTACAA ATTGTGGTCCTAGATTTACTATAATAAAAAAACTACCATATGATAGATGCAACACAACTATGAAAAAATTTGTTATGTAT AATAAGTGTAGAAGTGAATATTATGATAAATTAAATAGAAGATTTCATGCAGAGCCAACATGTTGTGTAAATTGTGGCCC TAAATTAAAGTTAGTTGATAGATTTGGAAGGAAAATAAAATGTGATGATGTTTTAAGAATTACTCGCCAATATTTAAAGG AAGGAAGAATAATAGCACTGAAAGGAATTGGAGGTTTTAATCTAATTTGTGATGGAAAAAATAAAAGTAGCATAGATGTT TTAAGAAAAAGGAAAAATAGACCCCAAAAACCTTTGGCGCTAATGATGCGAGACATTGATGTAGTAAATAAATATTGTTT TGTTAATGAGAAAGAAAATGAGATATTACAAGGTAGTAAAAAGCCAATAATTTTATTAAATAAAAGAGAGAATAATCTTC CCAAAAATATAGTTTTTAAAAATAATAAATTAGGAATAGTGCTTCCATATAGCCCGTTACATGTTTTGTTATTTGATAAT GAACTAGAGGTTTTAGTATTTACAAGTGGAAATATAAATCAAATGCCAATAGAGTATAAGAATGATGAAGCATTTAATAA TTTAAATGATATTGCAGATTATTTTCTTATTCATGATAGGGAAATAAATGCATCAATTGATGATTCTGTCGTGAAGGTGT TCATGAATAAAGAATTAGTTATAAGAAGTGGACGAGGATATGCACCTATTTCAATTAGAAGTAATAGTGAAGAAGGAGTT TTAGCATTAGGTTCTCAACTCAAAAATACTTTATCTATAAGTAGTGGTAATAACATTTTTATAAGTCCTTATGTTGGTGA TATGGAAAACTTAGAGACGATAGATAGATTTAAAAGAAATCTTAAGTTAATAAGAAGCTTATATGATATAAAAATTAATA CAATCTGTTATGATATGCATCCAAATTATTTTAATAATTACTTTTTAGAAGATAATTTAAATGATATGGATATTAAAGAA AGCAATATGAAGTTTAAAAAAATAGGAATATATCATCATCATGCTCATATTGCAAGTTGTATGTTTGATAATGATATACA AGAACCAGTAATAGGAATTGCTTTTGATGGGACTGGATATGGAGAAGATAAGAAAATATGGGGTGGAGAGTTTTTAATAT GTGATTATAAAAAGTTTAAAAGAGTTGGATGTCTTAAGTACATGAACTTACCTGGTAATGATGGTGGAATAAAGATGCCT TGGAAAATGGGAATGAGTCTTTTGAAAGGAGCTTTAAAAAATAATATTGTAAGTAAAAACCTAAATATATTTAAAAATTA TAATTTACCAGATTGCTTTAATTTAGAAAATTATGAATTAATAAGTAAAATTATAGATAGAAATATTAATACTTCTGAGA CAAGCAGTATGGGACGCTTATTTGATGGTGTAGCAAGTCTTTTAGGTTTTACGAATAATGTTACTTATGAGGGTGAAGCT GCGATATATTTAGAAAATATAGCTAGTACTTATATTATTAATAGTGTAGAAAGCGTTTATAAATTAAGCTATTCTTATAA TTTAAAGTTTAATGATGAAAAGTATATCATTGATTTAGATGAAATGATATTAGAAATTTTAGAGGATTTAAAAAATGAGG AGAGTTTAGGTTTAATATCTATAAAATTTCATAATACAATAACTGAATTTTCAATAGATTTATGCAAAAAGTTAAGGAAA CAATATAACATAAATAAAGTTGCTTTAAGTGGCGGGGTTTTTCAAAATGATATATTATTAAAAAATTTATATTTGAAATT AGAAGAAAATGATTTTAATGTATTAATTCACGGAGAGATACCTTGTAATGATAGTGGAATTTCATTGGGTCAGCTTATTA TAGCAAATGAGTTAATAAAAAGTCACAATTAA
Upstream 100 bases:
>100_bases AAAAAAATTGTCCTACATGTAAAACTTATAGTTCTAATATAGTAAGCGGTTATGAAACTATATTAGAGCAAATAGAAGGG GAATAATTTAGTGAAAATAT
Downstream 100 bases:
>100_bases AATTGAGGTGAAGAGATATGTGCGTGGCTATTCCGGGACAAATTATAGAAATTTTAGGGGAAGAATCTTTGGTTAAATTT GGAGGGATTAAAAAGCTTAT
Product: [NiFe] hydrogenase maturation protein HypF
Products: NA
Alternate protein names: Carbamoyl phosphate-converting enzyme hypF; [NiFe]-hydrogenase maturation factor hypF; Hydrogenase maturation protein hypF [H]
Number of amino acids: Translated: 783; Mature: 783
Protein sequence:
>783_residues MIKRKYILVNGRVQGVGFRPFVYRIACKNNIKGYVKNTCNGVQIDVEGLEENIKSFIMDLDSKCPKVCKINEIDINDIKY MNYYNDFQILESDNNYKGETLISPDIAICDECYKELFNISEERRYLYSFINCTNCGPRFTIIKKLPYDRCNTTMKKFVMY NKCRSEYYDKLNRRFHAEPTCCVNCGPKLKLVDRFGRKIKCDDVLRITRQYLKEGRIIALKGIGGFNLICDGKNKSSIDV LRKRKNRPQKPLALMMRDIDVVNKYCFVNEKENEILQGSKKPIILLNKRENNLPKNIVFKNNKLGIVLPYSPLHVLLFDN ELEVLVFTSGNINQMPIEYKNDEAFNNLNDIADYFLIHDREINASIDDSVVKVFMNKELVIRSGRGYAPISIRSNSEEGV LALGSQLKNTLSISSGNNIFISPYVGDMENLETIDRFKRNLKLIRSLYDIKINTICYDMHPNYFNNYFLEDNLNDMDIKE SNMKFKKIGIYHHHAHIASCMFDNDIQEPVIGIAFDGTGYGEDKKIWGGEFLICDYKKFKRVGCLKYMNLPGNDGGIKMP WKMGMSLLKGALKNNIVSKNLNIFKNYNLPDCFNLENYELISKIIDRNINTSETSSMGRLFDGVASLLGFTNNVTYEGEA AIYLENIASTYIINSVESVYKLSYSYNLKFNDEKYIIDLDEMILEILEDLKNEESLGLISIKFHNTITEFSIDLCKKLRK QYNINKVALSGGVFQNDILLKNLYLKLEENDFNVLIHGEIPCNDSGISLGQLIIANELIKSHN
Sequences:
>Translated_783_residues MIKRKYILVNGRVQGVGFRPFVYRIACKNNIKGYVKNTCNGVQIDVEGLEENIKSFIMDLDSKCPKVCKINEIDINDIKY MNYYNDFQILESDNNYKGETLISPDIAICDECYKELFNISEERRYLYSFINCTNCGPRFTIIKKLPYDRCNTTMKKFVMY NKCRSEYYDKLNRRFHAEPTCCVNCGPKLKLVDRFGRKIKCDDVLRITRQYLKEGRIIALKGIGGFNLICDGKNKSSIDV LRKRKNRPQKPLALMMRDIDVVNKYCFVNEKENEILQGSKKPIILLNKRENNLPKNIVFKNNKLGIVLPYSPLHVLLFDN ELEVLVFTSGNINQMPIEYKNDEAFNNLNDIADYFLIHDREINASIDDSVVKVFMNKELVIRSGRGYAPISIRSNSEEGV LALGSQLKNTLSISSGNNIFISPYVGDMENLETIDRFKRNLKLIRSLYDIKINTICYDMHPNYFNNYFLEDNLNDMDIKE SNMKFKKIGIYHHHAHIASCMFDNDIQEPVIGIAFDGTGYGEDKKIWGGEFLICDYKKFKRVGCLKYMNLPGNDGGIKMP WKMGMSLLKGALKNNIVSKNLNIFKNYNLPDCFNLENYELISKIIDRNINTSETSSMGRLFDGVASLLGFTNNVTYEGEA AIYLENIASTYIINSVESVYKLSYSYNLKFNDEKYIIDLDEMILEILEDLKNEESLGLISIKFHNTITEFSIDLCKKLRK QYNINKVALSGGVFQNDILLKNLYLKLEENDFNVLIHGEIPCNDSGISLGQLIIANELIKSHN >Mature_783_residues MIKRKYILVNGRVQGVGFRPFVYRIACKNNIKGYVKNTCNGVQIDVEGLEENIKSFIMDLDSKCPKVCKINEIDINDIKY MNYYNDFQILESDNNYKGETLISPDIAICDECYKELFNISEERRYLYSFINCTNCGPRFTIIKKLPYDRCNTTMKKFVMY NKCRSEYYDKLNRRFHAEPTCCVNCGPKLKLVDRFGRKIKCDDVLRITRQYLKEGRIIALKGIGGFNLICDGKNKSSIDV LRKRKNRPQKPLALMMRDIDVVNKYCFVNEKENEILQGSKKPIILLNKRENNLPKNIVFKNNKLGIVLPYSPLHVLLFDN ELEVLVFTSGNINQMPIEYKNDEAFNNLNDIADYFLIHDREINASIDDSVVKVFMNKELVIRSGRGYAPISIRSNSEEGV LALGSQLKNTLSISSGNNIFISPYVGDMENLETIDRFKRNLKLIRSLYDIKINTICYDMHPNYFNNYFLEDNLNDMDIKE SNMKFKKIGIYHHHAHIASCMFDNDIQEPVIGIAFDGTGYGEDKKIWGGEFLICDYKKFKRVGCLKYMNLPGNDGGIKMP WKMGMSLLKGALKNNIVSKNLNIFKNYNLPDCFNLENYELISKIIDRNINTSETSSMGRLFDGVASLLGFTNNVTYEGEA AIYLENIASTYIINSVESVYKLSYSYNLKFNDEKYIIDLDEMILEILEDLKNEESLGLISIKFHNTITEFSIDLCKKLRK QYNINKVALSGGVFQNDILLKNLYLKLEENDFNVLIHGEIPCNDSGISLGQLIIANELIKSHN
Specific function: Along with hypE, it catalyzes the synthesis of the CN ligands of the active site iron of [NiFe]-hydrogenases using carbamoylphosphate as a substrate. It functions as a carbamoyl transferase using carbamoylphosphate as a substrate and transferring the carb
COG id: COG0068
COG function: function code O; Hydrogenase maturation factor
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 YrdC-like domain [H]
Homologues:
Organism=Escherichia coli, GI2367152, Length=780, Percent_Identity=33.0769230769231, Blast_Score=408, Evalue=1e-115,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR001792 - InterPro: IPR017968 - InterPro: IPR004421 - InterPro: IPR017945 - InterPro: IPR006070 - InterPro: IPR011125 [H]
Pfam domain/function: PF00708 Acylphosphatase; PF01300 Sua5_yciO_yrdC; PF07503 zf-HYPF [H]
EC number: NA
Molecular weight: Translated: 90246; Mature: 90246
Theoretical pI: Translated: 8.02; Mature: 8.02
Prosite motif: PS00150 ACYLPHOSPHATASE_1 ; PS51160 ACYLPHOSPHATASE_3 ; PS51163 YRDC
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
2.9 %Cys (Translated Protein) 2.6 %Met (Translated Protein) 5.5 %Cys+Met (Translated Protein) 2.9 %Cys (Mature Protein) 2.6 %Met (Mature Protein) 5.5 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MIKRKYILVNGRVQGVGFRPFVYRIACKNNIKGYVKNTCNGVQIDVEGLEENIKSFIMDL CCCEEEEEEECEEECCCCCCEEEEEEECCCCCCHHHCCCCCEEEEHHHHHHHHHHHHHHH DSKCPKVCKINEIDINDIKYMNYYNDFQILESDNNYKGETLISPDIAICDECYKELFNIS HCCCCCCEEECCCCCCCEEEEEECCCEEEEECCCCCCCCEEECCCHHHHHHHHHHHHCCC EERRYLYSFINCTNCGPRFTIIKKLPYDRCNTTMKKFVMYNKCRSEYYDKLNRRFHAEPT HHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCEECCCCC CCVNCGPKLKLVDRFGRKIKCDDVLRITRQYLKEGRIIALKGIGGFNLICDGKNKSSIDV EEECCCCCCHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCEEEEECCCCCCHHHH LRKRKNRPQKPLALMMRDIDVVNKYCFVNEKENEILQGSKKPIILLNKRENNLPKNIVFK HHHHCCCCCCHHHHHHHHHHHHHHEEEECCCCCHHHCCCCCCEEEEECCCCCCCCCEEEE NNKLGIVLPYSPLHVLLFDNELEVLVFTSGNINQMPIEYKNDEAFNNLNDIADYFLIHDR CCCEEEEECCCCEEEEEECCCEEEEEEECCCCCCCCEEECCCHHHCCHHHHHHHHEEECC EINASIDDSVVKVFMNKELVIRSGRGYAPISIRSNSEEGVLALGSQLKNTLSISSGNNIF CCCCCCHHHHHHHHHCCEEEEECCCCEEEEEEECCCCCCHHHHHHHHHHEEEECCCCEEE ISPYVGDMENLETIDRFKRNLKLIRSLYDIKINTICYDMHPNYFNNYFLEDNLNDMDIKE EECCCCCHHHHHHHHHHHHHHHHHHHHHCEEEEEEEEECCCCHHCCEEECCCCCCCCCCC SNMKFKKIGIYHHHAHIASCMFDNDIQEPVIGIAFDGTGYGEDKKIWGGEFLICDYKKFK CCCEEEEEEEEEHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCEEECCEEEEECHHHHH RVGCLKYMNLPGNDGGIKMPWKMGMSLLKGALKNNIVSKNLNIFKNYNLPDCFNLENYEL HHCCEEEECCCCCCCCEECCHHHHHHHHHHHHHCCHHHCCCCEEECCCCCCCCCCCCHHH ISKIIDRNINTSETSSMGRLFDGVASLLGFTNNVTYEGEAAIYLENIASTYIINSVESVY HHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCEEEEEEHHHHHHHHHHHHHHH KLSYSYNLKFNDEKYIIDLDEMILEILEDLKNEESLGLISIKFHNTITEFSIDLCKKLRK EEEEEEEEEECCCEEEEEHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHH QYNINKVALSGGVFQNDILLKNLYLKLEENDFNVLIHGEIPCNDSGISLGQLIIANELIK HCCCCEEEEECCCEECCEEEEEEEEEEECCCEEEEEEECCCCCCCCCCHHHHHHHHHHHH SHN CCC >Mature Secondary Structure MIKRKYILVNGRVQGVGFRPFVYRIACKNNIKGYVKNTCNGVQIDVEGLEENIKSFIMDL CCCEEEEEEECEEECCCCCCEEEEEEECCCCCCHHHCCCCCEEEEHHHHHHHHHHHHHHH DSKCPKVCKINEIDINDIKYMNYYNDFQILESDNNYKGETLISPDIAICDECYKELFNIS HCCCCCCEEECCCCCCCEEEEEECCCEEEEECCCCCCCCEEECCCHHHHHHHHHHHHCCC EERRYLYSFINCTNCGPRFTIIKKLPYDRCNTTMKKFVMYNKCRSEYYDKLNRRFHAEPT HHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCEECCCCC CCVNCGPKLKLVDRFGRKIKCDDVLRITRQYLKEGRIIALKGIGGFNLICDGKNKSSIDV EEECCCCCCHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCEEEEECCCCCCHHHH LRKRKNRPQKPLALMMRDIDVVNKYCFVNEKENEILQGSKKPIILLNKRENNLPKNIVFK HHHHCCCCCCHHHHHHHHHHHHHHEEEECCCCCHHHCCCCCCEEEEECCCCCCCCCEEEE NNKLGIVLPYSPLHVLLFDNELEVLVFTSGNINQMPIEYKNDEAFNNLNDIADYFLIHDR CCCEEEEECCCCEEEEEECCCEEEEEEECCCCCCCCEEECCCHHHCCHHHHHHHHEEECC EINASIDDSVVKVFMNKELVIRSGRGYAPISIRSNSEEGVLALGSQLKNTLSISSGNNIF CCCCCCHHHHHHHHHCCEEEEECCCCEEEEEEECCCCCCHHHHHHHHHHEEEECCCCEEE ISPYVGDMENLETIDRFKRNLKLIRSLYDIKINTICYDMHPNYFNNYFLEDNLNDMDIKE EECCCCCHHHHHHHHHHHHHHHHHHHHHCEEEEEEEEECCCCHHCCEEECCCCCCCCCCC SNMKFKKIGIYHHHAHIASCMFDNDIQEPVIGIAFDGTGYGEDKKIWGGEFLICDYKKFK CCCEEEEEEEEEHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCEEECCEEEEECHHHHH RVGCLKYMNLPGNDGGIKMPWKMGMSLLKGALKNNIVSKNLNIFKNYNLPDCFNLENYEL HHCCEEEECCCCCCCCEECCHHHHHHHHHHHHHCCHHHCCCCEEECCCCCCCCCCCCHHH ISKIIDRNINTSETSSMGRLFDGVASLLGFTNNVTYEGEAAIYLENIASTYIINSVESVY HHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCEEEEEEHHHHHHHHHHHHHHH KLSYSYNLKFNDEKYIIDLDEMILEILEDLKNEESLGLISIKFHNTITEFSIDLCKKLRK EEEEEEEEEECCCEEEEEHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHH QYNINKVALSGGVFQNDILLKNLYLKLEENDFNVLIHGEIPCNDSGISLGQLIIANELIK HCCCCEEEEECCCEECCEEEEEEEEEEECCCEEEEEEECCCCCCCCCCHHHHHHHHHHHH SHN CCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 8590279; 8905231 [H]